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The map label for this gene is nqrF [H]

Identifier: 161869497

GI number: 161869497

Start: 530918

End: 532135

Strand: Reverse

Name: nqrF [H]

Synonym: NMCC_0510

Alternate gene names: 161869497

Gene position: 532135-530918 (Counterclockwise)

Preceding gene: 161869498

Following gene: 161869496

Centisome position: 24.71

GC content: 52.71

Gene sequence:

>1218_bases
ATGGAGATTATTTTAGGTATCGTGATGTTTACCGTCATCGTTTTGGTTTTGGCACTGATGATTCTGTTTGCCAAATCCAA
ACTGGTCAGCGAAGGCGACATCACCATCAAAGTCAACGGCGAAAAAGAGCTGACGATGCCCGCCGGCGGCAAACTCTTGG
GCGCGCTTGCCAACGAAGGCATCTTTATCCCCTCCGCCTGCGGCGGGGGCGGTTCGTGCGGACAATGCCGCGTCGTCGTA
AAAAGCGGCGGCGGCGACATCCTGCCGACCGAGCTGTCCCACATCAGCAAACGCGAAGCACGCGAAGGCTGCCGTCTGTC
GTGCCAAGTCAACGTCAAAACCGACATGGACATCGAAGTCCCCGAAGAAGTGTTCGGCGTGAAAAAATGGGAATGCACCG
TCATCTCCAACGATAACAAAGCCACGTTCATTAAAGAACTCAAGCTTGCCATTCCCGAAGGCGAAGAAGTCCCCTTCCGC
GCCGGCGGCTACATTCAAATTGAAGCCCCGCCGCACACCGTTGCCTACAAAGACTTCGACATTCCCGAGGAATACCACGA
AGACTGGGACAAATACAATTTGTGGCAATACGTTTCCAAAGTGGACGAGCCGATTTTGCGTGCCTACTCTATGGCTTCGT
ATCCTGAAGAAAAAGGCATCATTATGCTGAACGTGCGTATCGCCACGCCGCCCCCGCGCGTACCTGATGCGCCTCCGGGA
CAAATGTCGTCCTACATCTGGTCGCTCAAACCCGGCGACAAAGTTACGATTTCCGGCCCGTTCGGCGAATTTTTCGCCAA
AGACACCGATGCCGAAATGGTATTTATCGGCGGCGGTGCGGGTATGGCTCCGATGCGTTCCCACATTTTCGACCAGTTGA
AACGTTTGAACTCCAAACGTAAGATTACCTTCTGGTACGGCGCACGTTCCAAACGCGAAATGTTCTATGTCGAAGACTTC
GACCAACTCGCAGCAGAATTCCCGAACTTCACATGGCACGTCGCCCTGTCCGACCCATTGCCTGAAGACAACTGGGACGG
CTACACAGGCTTCATTCACAACGTGGTTTACGAAAACCATCTGAAAAACCACGAAGCGCCGGAAGACTGCGAATTTTATA
TGTGTGGTCCTCCGATCATGAACCAGTCCGTCATCAAAATGCTTAAAGACTTAGGCGTGGAAGACGAAAACATCCTCTTG
GACGATTTCGGCGGTTAA

Upstream 100 bases:

>100_bases
AAAGGCCTCAAAGGCTTGGGCATTACCTTTATCGCCGCCGGCCTGATGGCGATGGCGTTTATGTCGTTCTCTGGCATCCA
GTTATAAGAAAGGGATCGGT

Downstream 100 bases:

>100_bases
ATATTCGGCTTCAAACACAAAAGGAAGAAACCTCGGTTTCTTCCTTTTTCTATCCGATGCCGCCCGAACCCCCTTCAGAC
GGCATCGGCATAGAAACATA

Product: Na(+)-translocating NADH-quinone reductase subunit F

Products: @TETR01txt*tetrahydropteridine!; NO3-; NAD(P); Proton [C]

Alternate protein names: Na(+)-NQR subunit F; Na(+)-translocating NQR subunit F; NQR complex subunit F; NQR-1 subunit F [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKELTMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVV
KSGGGDILPTELSHISKREAREGCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
AGGYIQIEAPPHTVAYKDFDIPEEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEKGIIMLNVRIATPPPRVPDAPPG
QMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFIGGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDF
DQLAAEFPNFTWHVALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLGVEDENILL
DDFGG

Sequences:

>Translated_405_residues
MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKELTMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVV
KSGGGDILPTELSHISKREAREGCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
AGGYIQIEAPPHTVAYKDFDIPEEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEKGIIMLNVRIATPPPRVPDAPPG
QMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFIGGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDF
DQLAAEFPNFTWHVALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLGVEDENILL
DDFGG
>Mature_405_residues
MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKELTMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVV
KSGGGDILPTELSHISKREAREGCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
AGGYIQIEAPPHTVAYKDFDIPEEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEKGIIMLNVRIATPPPRVPDAPPG
QMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFIGGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDF
DQLAAEFPNFTWHVALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLGVEDENILL
DDFGG

Specific function: NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na(+) ions from the cytoplasm to the periplasm. The first step is catalyzed by nqrF, which accepts electrons from NADH and reduces

COG id: COG2871

COG function: function code C; Na+-transporting NADH:ubiquinone oxidoreductase, subunit NqrF

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=201, Percent_Identity=27.8606965174129, Blast_Score=69, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323552, Length=202, Percent_Identity=26.2376237623762, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323510, Length=154, Percent_Identity=25.3246753246753, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322146, Length=160, Percent_Identity=27.5, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR010205
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.34 [C]

Molecular weight: Translated: 45165; Mature: 45165

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKELTMPAGGKLLGALANEG
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCCHHHHHHHCCC
IFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREAREGCRLSCQVNVKTDMDIEV
EEEECCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCC
PEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFRAGGYIQIEAPPHTVAYKDFD
CHHHHCCCEEEEEEEECCCCCHHEEHHHHCCCCCCCCCEECCCEEEEECCCCEEEEECCC
IPEEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEKGIIMLNVRIATPPPRVPDAPPG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCC
QMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFIGGGAGMAPMRSHIFDQLKRLNSKR
CHHHEEEEECCCCEEEEECCCHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCC
KITFWYGARSKREMFYVEDFDQLAAEFPNFTWHVALSDPLPEDNWDGYTGFIHNVVYENH
EEEEEECCCCCCEEEEECCHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
LKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLGVEDENILLDDFGG
HCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCEEEECCCC
>Mature Secondary Structure
MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKELTMPAGGKLLGALANEG
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCCHHHHHHHCCC
IFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREAREGCRLSCQVNVKTDMDIEV
EEEECCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCC
PEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFRAGGYIQIEAPPHTVAYKDFD
CHHHHCCCEEEEEEEECCCCCHHEEHHHHCCCCCCCCCEECCCEEEEECCCCEEEEECCC
IPEEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEKGIIMLNVRIATPPPRVPDAPPG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCC
QMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFIGGGAGMAPMRSHIFDQLKRLNSKR
CHHHEEEEECCCCEEEEECCCHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCC
KITFWYGARSKREMFYVEDFDQLAAEFPNFTWHVALSDPLPEDNWDGYTGFIHNVVYENH
EEEEEECCCCCCEEEEECCHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
LKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLGVEDENILLDDFGG
HCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dihydropteridine; 2 nitric oxide; O2; NAD(P)H [C]

Specific reaction: NAD(P)H + dihydropteridine = NAD(P)+ @TETR01.txt*tetrahydropteridine! 2 nitric oxide + 2 O2 + NAD(P)H = 2 NO3- + NAD(P) + Proton [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA