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The map label for this gene is apbE [H]

Identifier: 161869496

GI number: 161869496

Start: 529711

End: 530646

Strand: Reverse

Name: apbE [H]

Synonym: NMCC_0509

Alternate gene names: 161869496

Gene position: 530646-529711 (Counterclockwise)

Preceding gene: 161869497

Following gene: 161869495

Centisome position: 24.64

GC content: 53.1

Gene sequence:

>936_bases
ATGGGCACGACCTATACCGTCAAATACCTTTCAAATAATCGGGACAAACTCCCCTCACCTGCCGAAATACAAAAGCGCAT
CGATGACGCGCTTAAAGAAGTCAACCGGCAGATGTCCACCTATCAGCCCGACTCCGAAATCAGCCGGTTCAACCAACACA
CAGCCGGCAAGCCCCTCCGCATTTCAAGCGACTTCGCACACGTTACTGCCGAAGCCGTCCGCCTGAACCGCCTGACACAC
GGCGCGCTGGACGTAACCGTCGGCCCCTTGGTCAACCTTTGGGGATTCGGCCCCGACAAATCCGTTACCCGTGAACCGTC
GCCGGAACAAATCAAACAAGCAGCATCTTATACGGGCATAGACAAAATCATTTTGAAACAAGGCAAAGATTACGCTTCCT
TGAGCAAAACCCACCCCAAGGCCTATTTGGATTTATCTTCGATTGCCAAAGGCTTCGGCGTTGATAAAGTTGCGGGCGAA
CTGGAAAAATACGGCATTCAAAATTATCTGGTCGAAATCGGCGGCGAGTTGCACGGCAAAGGCAAAAACGCGCGCGGCGA
ACCTTGGCGCATCGGCATCGAACAGCCCAACATCGTCCAAGGCGGCAATACGCAGATTATCGTCCCGCTGAACAACCGTT
CGCTTGCCACTTCCGGCGATTACCGTATTTTCCACGTCGATAAAAGCGGCAAACGCCTCTCCCATATCATTAATCCGAAC
AACAAACGACCCATCAGCCACAACCTCGCCTCCATCAGCGTGGTCGCAGACAGTGCGATGACGGCGGACGGCTTGTCCAC
AGGATTATTCGTATTGGGCGAAACCGAAGCCTTAAAGCTGGCAGAGCGCGAAAAACTCGCTGTTTTCCTGATTGTCAGGG
ATACAGGCGGCTACCGCACCGCCATGTCTTCCGAATTTGAAAAACTGCTCCGCTAA

Upstream 100 bases:

>100_bases
GCCGAACTTTATCCGCGTCTTGATATTTGCCCTGGGTTTCATCTTCCTGAACGCCTGTTCGGAACAAACCGCGCAAACCG
TTACCCTGCAAGGCGAAACG

Downstream 100 bases:

>100_bases
ACATACAAACCGATTAGGAAACACCATGAAAACCCTGCTCCTCACCTTCGGCATCTTCCTGACCGTCATCATCGGTATGG
CGGTCGGCTATATCTTCTCC

Product: thiamine biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MGTTYTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRISSDFAHVTAEAVRLNRLTH
GALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGIDKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGE
LEKYGIQNYLVEIGGELHGKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKSGKRLSHIINPN
NKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRDTGGYRTAMSSEFEKLLR

Sequences:

>Translated_311_residues
MGTTYTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRISSDFAHVTAEAVRLNRLTH
GALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGIDKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGE
LEKYGIQNYLVEIGGELHGKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKSGKRLSHIINPN
NKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRDTGGYRTAMSSEFEKLLR
>Mature_310_residues
GTTYTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRISSDFAHVTAEAVRLNRLTHG
ALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGIDKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGEL
EKYGIQNYLVEIGGELHGKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKSGKRLSHIINPNN
KRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRDTGGYRTAMSSEFEKLLR

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=290, Percent_Identity=38.9655172413793, Blast_Score=231, Evalue=4e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 34168; Mature: 34037

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTTYTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLR
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEE
ISSDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
ECCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCH
DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELHGK
HHHHHHCCCCHHHHHHCCCHHEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
GKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKSGKRLSHIINPN
CCCCCCCCEEEECCCCCEEECCCCEEEEEECCCEEECCCCEEEEEECCCCCHHHHHCCCC
NKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRDTGGYRT
CCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEECHHHHHHHCCCCEEEEEEEECCCCCHH
AMSSEFEKLLR
HHHHHHHHHHC
>Mature Secondary Structure 
GTTYTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLR
CCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEE
ISSDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
ECCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCH
DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELHGK
HHHHHHCCCCHHHHHHCCCHHEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
GKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKSGKRLSHIINPN
CCCCCCCCEEEECCCCCEEECCCCEEEEEECCCEEECCCCEEEEEECCCCCHHHHHCCCC
NKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRDTGGYRT
CCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEECHHHHHHHCCCCEEEEEEEECCCCCHH
AMSSEFEKLLR
HHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]