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The map label for this gene is nth [H]

Identifier: 161869460

GI number: 161869460

Start: 491132

End: 491803

Strand: Reverse

Name: nth [H]

Synonym: NMCC_0472

Alternate gene names: 161869460

Gene position: 491803-491132 (Counterclockwise)

Preceding gene: 161869461

Following gene: 161869459

Centisome position: 22.84

GC content: 53.42

Gene sequence:

>672_bases
GTGCGTTATAATGCCGACCGCACAAACCGACTGCACAAAAAAATGAACAGACACATCCGCCAAGAAATTTTCGAACGCTT
CCGCGCCGCCAACCCCCATCCGACCACCGAGCTGAATTTCAACTCCCCTTTCGAGCTTTTAATTGCCGTTCTGCTTTCGG
CGCAGGCGACCGATGTCGGCGTAAACAAGGCGACGGCGAAGCTGTTTCCGGTTGCCGATACGCCGCAGGCGATGCTGGAT
TTGGGTTTGGACGGCGTGATGGAATATACGAAAACCATCGGGTTGTATAAAACCAAGTCCAAGCACATCATGCAGACCTG
CCGCATCCTGCTGGAAAAATACAATGGCGAAGTGCCGGAAGACCGCGAGGCTTTGGAATCGTTGCCGGGCGTGGGGCGCA
AAACGGCAAACGTGGTATTGAACACAGCGTTCGGACACCCCGTTATGGCGGTCGATACGCATATTTTCCGCGTGTCCAAC
CGAACCAAAATCGCACCCGGGAAAGATGTGCGCGAAGTCGAAGACAAACTGATGCGCTTCATTCCTAAAGAATTTCTGAT
GGACGCGCACCACTGGCTGATTTTGCACGGACGCTACACCTGCAAGGCACTCAAACCGCAATGCCAAACCTGCATCATCA
ACGATTTGTGCGAATATCCCGCCAAAGCCTGA

Upstream 100 bases:

>100_bases
GCGGTTGAATCCGTGGACAAGCGGGCTGATGCTGGTTATCGGCAAAACGGCGCGTTACGCCTTCATTCTGTGGGGAATGC
AATATTACGCCGCCTGAACC

Downstream 100 bases:

>100_bases
CTGCCGCCGCCCTTCAAAATGAAACGCTTGAAAATTCGGACGGACGTTGTAGAATTTCAACCTATGCCGTCTGAAGCCGA
CAAAATGCGCTTCAGACGGC

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MRYNADRTNRLHKKMNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATDVGVNKATAKLFPVADTPQAMLD
LGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNGEVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSN
RTKIAPGKDVREVEDKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA

Sequences:

>Translated_223_residues
MRYNADRTNRLHKKMNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATDVGVNKATAKLFPVADTPQAMLD
LGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNGEVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSN
RTKIAPGKDVREVEDKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA
>Mature_223_residues
MRYNADRTNRLHKKMNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATDVGVNKATAKLFPVADTPQAMLD
LGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNGEVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSN
RTKIAPGKDVREVEDKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=178, Percent_Identity=30.8988764044944, Blast_Score=92, Evalue=3e-19,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=72.5961538461538, Blast_Score=325, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI17554540, Length=175, Percent_Identity=30.2857142857143, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6324530, Length=190, Percent_Identity=24.7368421052632, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=28.8135593220339, Blast_Score=95, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 25416; Mature: 25416

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYNADRTNRLHKKMNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATDVG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
VNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNGEVPE
CCCHHHEEECCCCCHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
DREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVEDKLMRF
HHHHHHHCCCCCCHHHHHEEECCCCCCCHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHH
IPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA
CCHHHHHCCCCEEEEECCHHHHHCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRYNADRTNRLHKKMNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATDVG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
VNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNGEVPE
CCCHHHEEECCCCCHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
DREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVEDKLMRF
HHHHHHHCCCCCCHHHHHEEECCCCCCCHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHH
IPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA
CCHHHHHCCCCEEEEECCHHHHHCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]