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The map label for this gene is htrB [H]

Identifier: 161869419

GI number: 161869419

Start: 443519

End: 444415

Strand: Reverse

Name: htrB [H]

Synonym: NMCC_0427

Alternate gene names: 161869419

Gene position: 444415-443519 (Counterclockwise)

Preceding gene: 161869423

Following gene: 161869414

Centisome position: 20.64

GC content: 54.29

Gene sequence:

>897_bases
ATGTTTCGTTTACAATTCGGGCTGTTTCCCCCTTTGCGAACCGCCATGCACATCCTGTTGACCGCCCTACTCAAATGCCT
CTCCCTGCTGCCGCTTTCCTGTCTGCACACGCTGGGAAACCGGCTCGGACATCTGGCGTTTTACCTTTTAAAGGAAGACC
GCGCGCGCATCGTCGCCAATATGTGTCAGGCAGGCATGAATCCCGACCCCAAAACGGTCAAAGCCGTTTTTGCGGAAACG
GCAAAAGGCGGTTTGGAACTTGCCCTCGCATTTTTCAAAAAACCGGAAGACATAGAAACAATGTTCAAGTCGGTACACGG
CTGGGAACACGTGCAGCAGGCTTTGGACAAGGGCGAAGGGCTGCTGTTCATCACGCCGCACATCGGCAGCTACGATTTGG
GCGGACGCTACATCAGCCAACGGCTGCCATTCCCCTTAACCGCCATGTATAAACCGCCGAAAATCAAAGCGATAGACAAA
ATCATGCAGGCGGGCAGGGTTCGCGGCAAAGGAAAAACCGCGCCTACCAGCATACAAGGGGTCAAACAAATCATCAAAGC
CCTGCGTTCGGGCGAAGCAACCATCGTCCTGCCCGACCACGTCCCCTCCCCTCAAGAAGGCGGGGAAGGCGTATGGGTGG
ATTTCTTCGGCAAACCTGCCTATACCATGACGCTGGCGGCAAAATTGGCACACGTCAAAGGCGTGAAAACCCTATTTTTC
TGCTGCGAACGCCTGCCTGGCGGACAAGGTTTCGATTTGCACATCCGCCCCGTCCAAGGGGAATTGAACGGCGACAAAGC
CCATGATGCCGCCGTGTTCAACCGCAATGCCGAATATTGGATACGCCGTTTTCCGACGCAGTATCTGTTTATGTACAACC
GCTACAAAATGCCGTAA

Upstream 100 bases:

>100_bases
CTTTTGAAAAGCAGGTTGGCATGGAATTCCCAACTCTTAATGCAGCGCGGATGGTAGCAGAAAAGCCTTTCCGCCGCAAT
ACTTCCCTTTTTCAGACGGC

Downstream 100 bases:

>100_bases
CGAAAATAAAAATGCCGTCTGAACAATTTCAGACGGCATTTTGTCATCTGACGATTTCCGACAGCGGCCAGCGCGGACGG
ACATTGAACGCACCGACCTC

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGHLAFYLLKEDRARIVANMCQAGMNPDPKTVKAVFAET
AKGGLELALAFFKKPEDIETMFKSVHGWEHVQQALDKGEGLLFITPHIGSYDLGGRYISQRLPFPLTAMYKPPKIKAIDK
IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGKPAYTMTLAAKLAHVKGVKTLFF
CCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRNAEYWIRRFPTQYLFMYNRYKMP

Sequences:

>Translated_298_residues
MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGHLAFYLLKEDRARIVANMCQAGMNPDPKTVKAVFAET
AKGGLELALAFFKKPEDIETMFKSVHGWEHVQQALDKGEGLLFITPHIGSYDLGGRYISQRLPFPLTAMYKPPKIKAIDK
IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGKPAYTMTLAAKLAHVKGVKTLFF
CCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRNAEYWIRRFPTQYLFMYNRYKMP
>Mature_298_residues
MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGHLAFYLLKEDRARIVANMCQAGMNPDPKTVKAVFAET
AKGGLELALAFFKKPEDIETMFKSVHGWEHVQQALDKGEGLLFITPHIGSYDLGGRYISQRLPFPLTAMYKPPKIKAIDK
IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGKPAYTMTLAAKLAHVKGVKTLFF
CCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRNAEYWIRRFPTQYLFMYNRYKMP

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=291, Percent_Identity=28.5223367697594, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082094, Length=273, Percent_Identity=26.7399267399267, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 33311; Mature: 33311

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGHLAFYLLKEDRARIVAN
CEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MCQAGMNPDPKTVKAVFAETAKGGLELALAFFKKPEDIETMFKSVHGWEHVQQALDKGEG
HHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHCCCC
LLFITPHIGSYDLGGRYISQRLPFPLTAMYKPPKIKAIDKIMQAGRVRGKGKTAPTSIQG
EEEECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHH
VKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGKPAYTMTLAAKLAHVKGVKTLFF
HHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
CCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRNAEYWIRRFPTQYLFMYNRYKMP
HHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHCCCHHHHHHHCCHHHHHHHHHCCCC
>Mature Secondary Structure
MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGHLAFYLLKEDRARIVAN
CEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MCQAGMNPDPKTVKAVFAETAKGGLELALAFFKKPEDIETMFKSVHGWEHVQQALDKGEG
HHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHCCCC
LLFITPHIGSYDLGGRYISQRLPFPLTAMYKPPKIKAIDKIMQAGRVRGKGKTAPTSIQG
EEEECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHH
VKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGKPAYTMTLAAKLAHVKGVKTLFF
HHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
CCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRNAEYWIRRFPTQYLFMYNRYKMP
HHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHCCCHHHHHHHCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]