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The map label for this gene is pbp3 [H]

Identifier: 161869423

GI number: 161869423

Start: 447082

End: 448629

Strand: Reverse

Name: pbp3 [H]

Synonym: NMCC_0431

Alternate gene names: 161869423

Gene position: 448629-447082 (Counterclockwise)

Preceding gene: 161869427

Following gene: 161869419

Centisome position: 20.83

GC content: 55.17

Gene sequence:

>1548_bases
GTGCGTCTCAATCATTTCATAATGATAGCGATTATTATATATGTGATTTCCCCTGCAAACAAGCCGGCCCGCCGCCACAG
CGTTCCCACTTATCCGGCTTTGCCTTATAATTGCTTTTTTTATGTAACAGATTTACCTATGAATTTCCCCAAAACAGCGG
CCTCCCTGCTGCTGCTTCTCGCCTCCCTCGCCGCACACGCGCTCGATACGGGTCGCATTCCGCAAAACGAAATCGCCGTA
TATGTCCAAGAGCTTGACAGCGGAAAAGTCATCATTGACCACCGCTCGGATGTCCCCGTCAACCCCGCCTCCACAATGAA
ACTCGTTACCGCGTTTGCCGCCTTCAAAACCTTCGGCAGCAATTACCGCTGGGCGACCGAGTTTAAAAGCAACGGTACGG
TAAACGACGGCACGCTTGACGGAAACCTGTATTGGGCGGGCAGCGGCGACCCCGTTTTCAATCAGGAAAACCTGCTTGCC
GTCCAACGCCAGTTGCGCGAACAAGGCATACGCAATATCACGGGACACCTGATGCTCGACCACAGCCTGTGGGGCGAAGT
CGGCAGCCCCGACGATTTCGAAGCCGACAGCGGTTCGCCGTTTATGACGCCCCCCAATCCAACTATGCTGTCTGCCGGTA
TGGTTATGGTGCGCGCCGAACGCAATGCCGCCGACAGTACCGACATCCTCACCGATCCGCCTTTGCCGCATATTTTCGCC
CAAAACAACTTGAAAATTACCGCCTCCCAAGCTGCCTGCCCTTCGATCAAAAAACTGATGCGTGCATCTTTTTCGGACAA
TACGCTGAAATTGCGCGGCAATATTCCCGAGAGCTGTTTGGGCAAGCCTGTCGGTGTCCGGATGTTCGCGCTTGACGAAC
TGATCCGGCAAAGTTTTACCAACCACTGGCTGCTCGGCGGCGGACGGATTTCAGACGGCATCGGCATATCCGACACGCCG
GAAGGCGCGCAGACGCTTGCCGTTGCACACTCAAAGCCGATGAAGGAAATTTTGACGGACATGAACAAGCGTTCGGACAA
TCTAATTGCGCGTTCCGTCTTCCTCAAACTCGGCGGCAACGGCAAACTGCCCGCCGTTTCCGAACAGGCAGCGTCTGCCG
TCCGGCGTGAACTTGCCGTGTCGGGCATCGATGTTGCGGATTTGGTTTTGGAAAACGGTTCAGGTCTGTCTAGAAAAGAA
AGGGTAACGGCGAGAATGATGGCGCAAATGTTGGAAACGGCTTATTTCAGCCCGTTTGCACAAGATTTCATCGATACGCT
GCCCATCGCCGGCACAGACGGGACTTTACGCAACCGCTTCAAACAAAGCGGCGGGCTGTTGCGCTTAAAAACCGGCACGC
TCAACAATGTCCGCGCCCTTGCAGGTTATTGGCTGGGCGACAAACCGATGGCGGTGGTCGTCATCATCAACAGCGGCCGC
GCCGTTTCCCTGCTGCCCGACTTGGACAACTTCGTTGCCAACAACATCATCTCCGGCGGCGATGGCTGGCTGGATGCGAA
ACTGATGTGCAAAGAACGCCGAGCCTGA

Upstream 100 bases:

>100_bases
GCATTCAATGCCACTTTGCGCGCAAAAGAAGCACGGCGCAGGCTGCCCACCAAAATACTGATTTTCTTAGCCATAATCAT
TCCTCCTGAATATTAAGTTT

Downstream 100 bases:

>100_bases
AACAGGAAAATATAGTGGATTAAATTTAAGGGGCTGTCCTAGATAACTAGGATAAACTCGATTTTACTAATTGTTTTAAA
ATGGAACAAGAACTTTTATC

Product: penicillin-binding protein 3

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 4a; PBP-4a [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MRLNHFIMIAIIIYVISPANKPARRHSVPTYPALPYNCFFYVTDLPMNFPKTAASLLLLLASLAAHALDTGRIPQNEIAV
YVQELDSGKVIIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGSGDPVFNQENLLA
VQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPNPTMLSAGMVMVRAERNAADSTDILTDPPLPHIFA
QNNLKITASQAACPSIKKLMRASFSDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGISDTP
EGAQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGNGKLPAVSEQAASAVRRELAVSGIDVADLVLENGSGLSRKE
RVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRNRFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGR
AVSLLPDLDNFVANNIISGGDGWLDAKLMCKERRA

Sequences:

>Translated_515_residues
MRLNHFIMIAIIIYVISPANKPARRHSVPTYPALPYNCFFYVTDLPMNFPKTAASLLLLLASLAAHALDTGRIPQNEIAV
YVQELDSGKVIIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGSGDPVFNQENLLA
VQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPNPTMLSAGMVMVRAERNAADSTDILTDPPLPHIFA
QNNLKITASQAACPSIKKLMRASFSDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGISDTP
EGAQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGNGKLPAVSEQAASAVRRELAVSGIDVADLVLENGSGLSRKE
RVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRNRFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGR
AVSLLPDLDNFVANNIISGGDGWLDAKLMCKERRA
>Mature_515_residues
MRLNHFIMIAIIIYVISPANKPARRHSVPTYPALPYNCFFYVTDLPMNFPKTAASLLLLLASLAAHALDTGRIPQNEIAV
YVQELDSGKVIIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGSGDPVFNQENLLA
VQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPNPTMLSAGMVMVRAERNAADSTDILTDPPLPHIFA
QNNLKITASQAACPSIKKLMRASFSDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGISDTP
EGAQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGNGKLPAVSEQAASAVRRELAVSGIDVADLVLENGSGLSRKE
RVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRNRFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGR
AVSLLPDLDNFVANNIISGGDGWLDAKLMCKERRA

Specific function: Catalyzes DD-carboxypeptidase and transpeptidation reactions [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Secreted (Probable). Note=May be anchored in the membrane via a C-terminal amphiphatic alpha helix [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=412, Percent_Identity=25.7281553398058, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 56013; Mature: 56013

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLNHFIMIAIIIYVISPANKPARRHSVPTYPALPYNCFFYVTDLPMNFPKTAASLLLLL
CCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHH
ASLAAHALDTGRIPQNEIAVYVQELDSGKVIIDHRSDVPVNPASTMKLVTAFAAFKTFGS
HHHHHHHHCCCCCCHHHHEEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCC
NYRWATEFKSNGTVNDGTLDGNLYWAGSGDPVFNQENLLAVQRQLREQGIRNITGHLMLD
CCEEEEEECCCCCCCCCEECCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
HSLWGEVGSPDDFEADSGSPFMTPPNPTMLSAGMVMVRAERNAADSTDILTDPPLPHIFA
HHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEEEECCCCCCCCCCCCCCCCCCEEE
QNNLKITASQAACPSIKKLMRASFSDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFT
CCCEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCHHHCCCCCCCHHHHHHHHHHHHCC
NHWLLGGGRISDGIGISDTPEGAQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGN
CCEEEECCEECCCCCCCCCCCCHHEEEECCCCCHHHHHHHHCCCCCHHHEEEEEEEECCC
GKLPAVSEQAASAVRRELAVSGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFA
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHCCHHH
QDFIDTLPIAGTDGTLRNRFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGR
HHHHHHCCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHEEECCCCEEEEEEEECCC
AVSLLPDLDNFVANNIISGGDGWLDAKLMCKERRA
EEEECCCHHHHHHHCCCCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MRLNHFIMIAIIIYVISPANKPARRHSVPTYPALPYNCFFYVTDLPMNFPKTAASLLLLL
CCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHH
ASLAAHALDTGRIPQNEIAVYVQELDSGKVIIDHRSDVPVNPASTMKLVTAFAAFKTFGS
HHHHHHHHCCCCCCHHHHEEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCC
NYRWATEFKSNGTVNDGTLDGNLYWAGSGDPVFNQENLLAVQRQLREQGIRNITGHLMLD
CCEEEEEECCCCCCCCCEECCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
HSLWGEVGSPDDFEADSGSPFMTPPNPTMLSAGMVMVRAERNAADSTDILTDPPLPHIFA
HHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEEEECCCCCCCCCCCCCCCCCCEEE
QNNLKITASQAACPSIKKLMRASFSDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFT
CCCEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCHHHCCCCCCCHHHHHHHHHHHHCC
NHWLLGGGRISDGIGISDTPEGAQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGN
CCEEEECCEECCCCCCCCCCCCHHEEEECCCCCHHHHHHHHCCCCCHHHEEEEEEEECCC
GKLPAVSEQAASAVRRELAVSGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFA
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHCCHHH
QDFIDTLPIAGTDGTLRNRFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGR
HHHHHHCCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHEEECCCCEEEEEEEECCC
AVSLLPDLDNFVANNIISGGDGWLDAKLMCKERRA
EEEECCCHHHHHHHCCCCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7711903; 9384377 [H]