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The map label for this gene is yafJ [C]

Identifier: 161869405

GI number: 161869405

Start: 424668

End: 425522

Strand: Reverse

Name: yafJ [C]

Synonym: NMCC_0413

Alternate gene names: 161869405

Gene position: 425522-424668 (Counterclockwise)

Preceding gene: 161869406

Following gene: 161869404

Centisome position: 19.76

GC content: 58.71

Gene sequence:

>855_bases
TTGGGACAAACCATGTGTCAACTGCTGGGCATGAACTGCAATACGCCGACCGATATTATGTTTTCCTTTGAAGGCTTCCG
CCGCAGGGGCGGCATTACCGACCACCATGCCGACGGTTTCGGCATCGGCTTTTTCGAAGGCAAAGGCGTGCGCCTGTTCC
ATGACGACAAACCCAGTGCCAATTCGCCCGTCGCCGACCTCGTGCGCGCCTACCAGATTAAATCGGAAAACGTCGTCGCC
CATATCCGCAAAGCATCACAAGGACAAACCTCGCTGGCGAACACCCATCCCTTTATGCGCGAAATGTGGGGCGGCTACTG
GCTGTTTGCCCACAACGGACATTTGATTGATTTTTTCCCCGAACAGGGCGAATTTTTCCACCCCGTCGGCACAACCGATT
CCGAACGCGCGTTCTGCCACATCCTCAACCGCCTGCGCACCCGCTTTGCCGCCCGTCCCGACGACGACACGCTGTTTGAC
GCGATTGCGGGGCTGACGCACGAAATCCGCAAGTTCGGGCTGTTTAACTTTATGCTTTCAGACGGCATTGCCCTGTTTGC
CCACGCCAGCACACTGCTGCACTACATCGTCCGCCAAGCCCCGTTCGGCAAGGCGCGCCTGCTCGACGACGACGTGATGG
TCGATTTTGCCGAAGTAACCACGCCCTCCGACCGCGTCGCCGTTATCGCCACCCTGCCGCTGACCCGCGATGAAACGTGG
GCGCAACTTGCCGTGAACGAACTGGTCATGTTCCGCGAAGGCAACATCGTCCGACACGACCGTCCCGAAAACCCCGTCTA
TATGAGTGCCGAAGAAGGTCTGGAAATCGCCCGCGCCGCCGGCGTATCGGTTTGA

Upstream 100 bases:

>100_bases
TCTGTTTTCTGTTTTCGAGGGAATAACGGGGAACAAGCCGTATTTCAGACGGCATTTTCAGTTCGGGGTATAATCCGAAT
ACTTGCGACCATCTGAATCA

Downstream 100 bases:

>100_bases
CGTTCAGACGGCATCAGGAAGAAACGCTATGAAATGCCCGTTTTGCGCCCACCCCGACACCCGCGTCGCCGATTCGCGTC
TGATGGAAGAACGCAACGCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MGQTMCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGKGVRLFHDDKPSANSPVADLVRAYQIKSENVVA
HIRKASQGQTSLANTHPFMREMWGGYWLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFD
AIAGLTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTPSDRVAVIATLPLTRDETW
AQLAVNELVMFREGNIVRHDRPENPVYMSAEEGLEIARAAGVSV

Sequences:

>Translated_284_residues
MGQTMCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGKGVRLFHDDKPSANSPVADLVRAYQIKSENVVA
HIRKASQGQTSLANTHPFMREMWGGYWLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFD
AIAGLTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTPSDRVAVIATLPLTRDETW
AQLAVNELVMFREGNIVRHDRPENPVYMSAEEGLEIARAAGVSV
>Mature_283_residues
GQTMCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGKGVRLFHDDKPSANSPVADLVRAYQIKSENVVAH
IRKASQGQTSLANTHPFMREMWGGYWLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDA
IAGLTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTPSDRVAVIATLPLTRDETWA
QLAVNELVMFREGNIVRHDRPENPVYMSAEEGLEIARAAGVSV

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=254, Percent_Identity=52.755905511811, Blast_Score=273, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 31848; Mature: 31716

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQTMCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGKGVRLFHDDKPSA
CCHHHHHHHCCCCCCCCCEEEEEHHHHHCCCCCCCCCCCCEEEEECCCCEEEEECCCCCC
NSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGYWLFAHNGHLIDFFP
CCCHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHCCHHHHHHCCCEEEEEECCEEEEEEC
EQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAGLTHEIRKFGLFNFMLS
CCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTPSDRVAVIATLPLTRDETW
CCHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHEEHHHHCCCCCCEEEEEEECCCCCHHH
AQLAVNELVMFREGNIVRHDRPENPVYMSAEEGLEIARAAGVSV
HHHHHHHHHEEECCCEEECCCCCCCEEEEHHHCHHHHHHCCCCC
>Mature Secondary Structure 
GQTMCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGKGVRLFHDDKPSA
CHHHHHHHCCCCCCCCCEEEEEHHHHHCCCCCCCCCCCCEEEEECCCCEEEEECCCCCC
NSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGYWLFAHNGHLIDFFP
CCCHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHCCHHHHHHCCCEEEEEECCEEEEEEC
EQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAGLTHEIRKFGLFNFMLS
CCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTPSDRVAVIATLPLTRDETW
CCHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHEEHHHHCCCCCCEEEEEEECCCCCHHH
AQLAVNELVMFREGNIVRHDRPENPVYMSAEEGLEIARAAGVSV
HHHHHHHHHEEECCCEEECCCCCCCEEEEHHHCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]