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The map label for this gene is pglD [H]

Identifier: 161869396

GI number: 161869396

Start: 413321

End: 415231

Strand: Reverse

Name: pglD [H]

Synonym: NMCC_0403

Alternate gene names: 161869396

Gene position: 415231-413321 (Counterclockwise)

Preceding gene: 161869397

Following gene: 161869395

Centisome position: 19.28

GC content: 56.57

Gene sequence:

>1911_bases
ATGACTCTGGAAACCCTGATCGCCCTGCCGCGCAACATCAAGAAAATCTGTTTCCTCATACACGATTTTCTGATGATTTT
CATTGCATTCTGGTTCACCCAAAGCCTAAAGGCCGACTACTCGAACGAATGGTTCGACCCTGTCAACTGGCAGTCTTTCC
TATTGACCGCGCTGTTGACCATCGGCCTGTTCGTCAGAATGGGGCTCTACCGTGCCGTTACACGCTTTGTCAGTTTCCAC
GTCCTGACCACCGCTTTTGCCGGCAGCATTGTTTCCGCCGTCCTGTTCTTCCTCAACACATTGATTTTTGAAGAAAGGCT
GCGCCTCGCCCTGCCGATTGTCTATTTCTTACTGCTGTTTGTTTCCGTGACCGGCTCGCGTATGGTTTTGCGCGGACTGT
TGTCCGAACACCCCAAAAAACAGATGATCCCTGTCATCATTTACGGCGCAGGGCAGGCGGGCAGACAACTGCTTGAGGCC
GTCAAACAGATACGCGAATATTCCGCGGTCGCCTTTGTGGACGACAACCCCAAACTGTGGCACACCGTCATCTACGACCT
TGCCGTTTACCAGCCCGATGCCATCGCCTTCCTCATCGAACGCTACGGCGTGGAAAAAATCCTACTCGCCATCCCCGGCG
CGACCCAGGAACAACGCCGCCGAATCATCAACAAACTGGAAGCCTATCCGTGCGAAGTGTTGACCATTCCCGGAATGAAA
GACCTGATGGACGGAAAAATCAGCATCGGCACGCTCAAAAAAATCTCTGTGTCCGACCTGCTCGGGCGTGATTCCGTCGC
GCCCGACGACCGCCTGATGAGTGCCGACATCGAAGGCAAAACCGTCATGGTAACCGGCGCGAGCGGCTCCATCGGTTCGG
AACTCTGCCGCCAGATTATCCGCCGCCGCCCCGAAAAGCTGCTGCTGTTCGAGTTATCCGAATTCGCCCTGTACGCCATC
GAAAAAGAATTGCGCGAAACCTGCATCCAAAAACGCCTCGACACCGAAATCCTGCCCTTTCTCGGTTCGGTGCAAAACCG
CACGCTGCTCGAACACGTCATGACCGCCTTTTCCGTTGCGACCGTCTATCACGCCGCTGCCTACAAACACGTCCCCATGG
TCGAGTTCAACACCGTCGAAGGCATACGCAACAACATCTTCGGCACACTCGAGTGCGCGCTTGCCGCCACGACATCGGGC
GTGGAAACTTTCGTCCTCATCTCCACCGACAAAGCCGTCCGCCCCACCAACACCATGGGTGCCAGCAAACGCATGGCGGA
ACTCTGCCTTCAGGCACTCGCCGCCGAACCCGGACAAAAAACCCGCTTCAGCATGGTACGTTTCGGCAATGTTTTAGGTT
CGTCCGGCTCCGTTGTCCCGCTGTTTGAAAAACAGATTGCAGAAGGCGGCCCGCTTACCCTGACCCACCCCGAAATCACA
CGTTATTTCATGACCATACCCGAAGCGGCGCAACTGGTTATCCAAGCCGGCGCGATGGGCAAAGGCGGCGACGTGTTCGT
CCTCGATATGGGCGAATCCGTCAAAATCATCGACCTTGCCCGCCAAATGATTACCTTAAGCGGACTCAAACCCAAAACGC
CCGAACAACCCGACGGCGACATCGAAATCCTCATTACCGGGCTGCGTCCCGGCGAAAAACTCTACGAAGAGCTGCTCATC
GGCGACAACGTCCGCAAAACCGGCCATCCGCGCATCATGACCGCCAACGAGACCATGCTGCCGTGGCACGAGCTCTCCGC
CCTGCTCGACCGCATCCGTGCGGCCTGCGACCGTTACGACCAGCAGGCAATCCGCACCCTGCTCATCAACGCCCCGACCG
GCTTTGCCCCGAGCGACGGCATCTGCGACCTGCTTTGGGTACGAGAAACACACAGAAAAAATGCCGTCTGA

Upstream 100 bases:

>100_bases
TCGCGTTCTGCAAAAAACACATCGAAGCCGTATTGGCAGAAGCCACACAATAACCCTTCAGACGGCATATGCCGCCTGAA
AGCACATACCGCCCCACGAT

Downstream 100 bases:

>100_bases
ACCTTCAGACGGCATAACGTACAAACCAACCTACCTTACACACACGGAGTTTGACATGCAGTTTTCAGCATTCGGCGAAA
AATTCACGCAACACAGCGGC

Product: pilin glycosylation protein

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 636; Mature: 635

Protein sequence:

>636_residues
MTLETLIALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSNEWFDPVNWQSFLLTALLTIGLFVRMGLYRAVTRFVSFH
VLTTAFAGSIVSAVLFFLNTLIFEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGQAGRQLLEA
VKQIREYSAVAFVDDNPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQRRRIINKLEAYPCEVLTIPGMK
DLMDGKISIGTLKKISVSDLLGRDSVAPDDRLMSADIEGKTVMVTGASGSIGSELCRQIIRRRPEKLLLFELSEFALYAI
EKELRETCIQKRLDTEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLECALAATTSG
VETFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGNVLGSSGSVVPLFEKQIAEGGPLTLTHPEIT
RYFMTIPEAAQLVIQAGAMGKGGDVFVLDMGESVKIIDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLI
GDNVRKTGHPRIMTANETMLPWHELSALLDRIRAACDRYDQQAIRTLLINAPTGFAPSDGICDLLWVRETHRKNAV

Sequences:

>Translated_636_residues
MTLETLIALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSNEWFDPVNWQSFLLTALLTIGLFVRMGLYRAVTRFVSFH
VLTTAFAGSIVSAVLFFLNTLIFEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGQAGRQLLEA
VKQIREYSAVAFVDDNPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQRRRIINKLEAYPCEVLTIPGMK
DLMDGKISIGTLKKISVSDLLGRDSVAPDDRLMSADIEGKTVMVTGASGSIGSELCRQIIRRRPEKLLLFELSEFALYAI
EKELRETCIQKRLDTEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLECALAATTSG
VETFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGNVLGSSGSVVPLFEKQIAEGGPLTLTHPEIT
RYFMTIPEAAQLVIQAGAMGKGGDVFVLDMGESVKIIDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLI
GDNVRKTGHPRIMTANETMLPWHELSALLDRIRAACDRYDQQAIRTLLINAPTGFAPSDGICDLLWVRETHRKNAV
>Mature_635_residues
TLETLIALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSNEWFDPVNWQSFLLTALLTIGLFVRMGLYRAVTRFVSFHV
LTTAFAGSIVSAVLFFLNTLIFEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGQAGRQLLEAV
KQIREYSAVAFVDDNPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQRRRIINKLEAYPCEVLTIPGMKD
LMDGKISIGTLKKISVSDLLGRDSVAPDDRLMSADIEGKTVMVTGASGSIGSELCRQIIRRRPEKLLLFELSEFALYAIE
KELRETCIQKRLDTEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLECALAATTSGV
ETFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGNVLGSSGSVVPLFEKQIAEGGPLTLTHPEITR
YFMTIPEAAQLVIQAGAMGKGGDVFVLDMGESVKIIDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIG
DNVRKTGHPRIMTANETMLPWHELSALLDRIRAACDRYDQQAIRTLLINAPTGFAPSDGICDLLWVRETHRKNAV

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 70919; Mature: 70788

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLETLIALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSNEWFDPVNWQSFLLTALLT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
IGLFVRMGLYRAVTRFVSFHVLTTAFAGSIVSAVLFFLNTLIFEERLRLALPIVYFLLLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGQAGRQLLEAVKQIREYSAVAFVDDNPKLW
HHCCCHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCEEEEECCCCCHH
HTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQRRRIINKLEAYPCEVLTIPGMK
HHHHHHHHHCCCHHHHHHHHHHCHHEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCHH
DLMDGKISIGTLKKISVSDLLGRDSVAPDDRLMSADIEGKTVMVTGASGSIGSELCRQII
HHHCCCEEECCCCCCHHHHHHCCCCCCCCCCEEECCCCCCEEEEECCCCCHHHHHHHHHH
RRRPEKLLLFELSEFALYAIEKELRETCIQKRLDTEILPFLGSVQNRTLLEHVMTAFSVA
HCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHHHH
TVYHAAAYKHVPMVEFNTVEGIRNNIFGTLECALAATTSGVETFVLISTDKAVRPTNTMG
HHHHHHHHCCCCCEEEHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCC
ASKRMAELCLQALAAEPGQKTRFSMVRFGNVLGSSGSVVPLFEKQIAEGGPLTLTHPEIT
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHCCCCCEEEECHHHH
RYFMTIPEAAQLVIQAGAMGKGGDVFVLDMGESVKIIDLARQMITLSGLKPKTPEQPDGD
HHHHHCCHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
IEILITGLRPGEKLYEELLIGDNVRKTGHPRIMTANETMLPWHELSALLDRIRAACDRYD
EEEEEECCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
QQAIRTLLINAPTGFAPSDGICDLLWVRETHRKNAV
HHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
TLETLIALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSNEWFDPVNWQSFLLTALLT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
IGLFVRMGLYRAVTRFVSFHVLTTAFAGSIVSAVLFFLNTLIFEERLRLALPIVYFLLLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGQAGRQLLEAVKQIREYSAVAFVDDNPKLW
HHCCCHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCEEEEECCCCCHH
HTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQRRRIINKLEAYPCEVLTIPGMK
HHHHHHHHHCCCHHHHHHHHHHCHHEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCHH
DLMDGKISIGTLKKISVSDLLGRDSVAPDDRLMSADIEGKTVMVTGASGSIGSELCRQII
HHHCCCEEECCCCCCHHHHHHCCCCCCCCCCEEECCCCCCEEEEECCCCCHHHHHHHHHH
RRRPEKLLLFELSEFALYAIEKELRETCIQKRLDTEILPFLGSVQNRTLLEHVMTAFSVA
HCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHHHH
TVYHAAAYKHVPMVEFNTVEGIRNNIFGTLECALAATTSGVETFVLISTDKAVRPTNTMG
HHHHHHHHCCCCCEEEHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCC
ASKRMAELCLQALAAEPGQKTRFSMVRFGNVLGSSGSVVPLFEKQIAEGGPLTLTHPEIT
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHCCCCCEEEECHHHH
RYFMTIPEAAQLVIQAGAMGKGGDVFVLDMGESVKIIDLARQMITLSGLKPKTPEQPDGD
HHHHHCCHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
IEILITGLRPGEKLYEELLIGDNVRKTGHPRIMTANETMLPWHELSALLDRIRAACDRYD
EEEEEECCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
QQAIRTLLINAPTGFAPSDGICDLLWVRETHRKNAV
HHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]