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The map label for this gene is ptrB [C]

Identifier: 161869338

GI number: 161869338

Start: 344273

End: 346288

Strand: Reverse

Name: ptrB [C]

Synonym: NMCC_0344

Alternate gene names: 161869338

Gene position: 346288-344273 (Counterclockwise)

Preceding gene: 161869339

Following gene: 161869336

Centisome position: 16.08

GC content: 58.23

Gene sequence:

>2016_bases
ATGAAATCCTACCCCGACCCCTACCGCCATTTTGAAAACCTCGATTCCGCCGAAACGCAAAACTTCGCTGCTGAAGCGAA
TGCCGAAACGCGCGCGCGTTTTTTAAACAACGACAAGGCGCGCGCGCTTTCAGACGGCATTTTGAATCAAATGCAGGACA
CGCGGCAGATTCCGTTTTGTCAGGAACACCGCGCGCGGATGTACCATTTCCATCAGGACGCGGAATATCCGAAGGGCGTG
TACCGCGTGTGTACGGCGGCGACGTACCGTTCCGGCTATCCCGAGTGGAAAATCCTGTTTTCAGTGGCGGATTTCGACGA
GCTGCTCGGCGACGATGTATATCTAGGCGGCGTGTCGCACTTGGTGGAAAAGCCCAACCGCGCACTGTTAACACTAAGCA
AATCAGGCGGCGATACGGCGTACACGCTGGAAGTGGATTTGGAAGCAGGGGAGTTGGTCGAAGGCGGTTTTCACTTTCCG
GCAGGCAAAAACCATGTGTCGTGGCGCGATGAAAACAGCGTGTGGGTGTGTCCGGCTTGGGACGAACGCCAGTTGACCGA
ATCGGGCTATCCGCGCGAGGTGTGGCTGGTGGAACGCGGCAAGAGTTTCGAGGAAAGCCTGCCTGTGTATCAAATCGATA
AAGGTGCAATGATGGTGAACGCGTGGCGTTATCTCGATCCGCAAGGTTCGCCGATTGATTTGATTGAAGCGTCGGACGGT
TTTTACACCAAAACCTATTTGCAGGTGTCGTCGGAAGGCGAGGCGAAACCGTTAAACCTGCCCAACGATTGCGACGTGGC
CGGCTATCTAGCCGGACATCTTTTGCTGACATTGCGTAAAGACTGGCACCGCGCGAACCAAAGCTATCCGAGCGGCGCGC
TGGTAGCGGTGAAACTGAATCGCGGCGAAATCGGGGCGGCGCAGCTTTTGTTTGCGCCCGATGAAACGCAGGCATTGGAA
AGCGTGGAAACGACCAAGCGTTTTGTGGTGGCGAGCCTGCTGGAGAACGTACAAGGCCGTCTGAAAGCGTGGCGTTTTGC
CGACGGCAAATGGCAGGAAGTCGAACTGCCGCGCCTGCCTTCGGGCGCGTTGGAAATGACCGACCAGCCTTGGGGCGGCG
ACGTGGTTTACCTTGCCGCCGGCGATTTCACCACACCGCTGACGCTGTTTGCGCTGGATTTGAACGTGATGGAACTGACC
GTCATGCGCCGCCAGCCGCAGCAGTTCGATTCAGACGGCATCGAAGTGCGGCAGTTTTGGACGACTTCTGCCGACGGCGA
GCGCATTCCTTATTTCCACGTCGGCAAAAACGCCGCGCCCGATACGCCGACTTTGGTCTATGCCTACGGCGGTTTCGGTA
TTCCCGAATTGCCGCATTATCTGGGCAGCATCGGCAAATACTGGCTGGAAGAGGGCAACGCCTTCGTGTTGGCGAACATA
CGCGGCGGCGGCGAGTTCGGCCCGCGCTGGCATCAGGCGGCGCAGGGCATCAGCAAACACAAAAGCGTCGACGATTTGCT
TGCCGTTGTGCGCGATTTGTCCGAACGCGGCATGAGTTTGCCCGAACACATCGGTTTGCAGGGCGGCAGCAACGGCGGCC
TGATTACCGCCGCCGCCTTCGTGCGCGAACCGCAAAGCATCGGCGCGCTGGTGTGCGAAGTACCGCTGACCGACATGATC
CGCTATCCGCTGCTCTCCGCCGGCGCAAGCTGGACGGACGAATACGGCAATCCGCAGAAATACGAAGTCTGCAAACGCCA
GTTGGGCGAATTGTCGCCGTATCACAATCTTTCAGACGGCATCGATTATCCGCCCGCACTCATCACCACCAGCCTCAGCG
ACGACCGCGTCCATCCCGCCCACGCGCTCAAGTTTTACGCCAAACTGCGCGAAACCTCGCCGCAATCTTGGCTCTACTCG
CCTGACGGCGGCGGCCATACCGGCAACGGCACGCAACGCGAATCCGCCGACGAACTCGCCTGCGTGTTGCTGTTTTTGAA
AGAGTTTTTGGGCTAA

Upstream 100 bases:

>100_bases
GCTGACCGCAACGGAAAGCCGCCGCAGAAATGCCGTCTGAACCCCGTTTCAGACGGCATTTCCCCGATTATAAAGACCTG
CCCAACCCTCAAGGACCCCG

Downstream 100 bases:

>100_bases
GGGCGGGGAGCGGCACTGCCGCCGCGAATGAAAAAAGGTCGTCTGAAACTGCTTTTTCAGACGACCTTTTTTAATGGTTG
TTTCAAATCAAAATATCTAT

Product: prolyl endopeptidase

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 671; Mature: 671

Protein sequence:

>671_residues
MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLNNDKARALSDGILNQMQDTRQIPFCQEHRARMYHFHQDAEYPKGV
YRVCTAATYRSGYPEWKILFSVADFDELLGDDVYLGGVSHLVEKPNRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIDKGAMMVNAWRYLDPQGSPIDLIEASDG
FYTKTYLQVSSEGEAKPLNLPNDCDVAGYLAGHLLLTLRKDWHRANQSYPSGALVAVKLNRGEIGAAQLLFAPDETQALE
SVETTKRFVVASLLENVQGRLKAWRFADGKWQEVELPRLPSGALEMTDQPWGGDVVYLAAGDFTTPLTLFALDLNVMELT
VMRRQPQQFDSDGIEVRQFWTTSADGERIPYFHVGKNAAPDTPTLVYAYGGFGIPELPHYLGSIGKYWLEEGNAFVLANI
RGGGEFGPRWHQAAQGISKHKSVDDLLAVVRDLSERGMSLPEHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMI
RYPLLSAGASWTDEYGNPQKYEVCKRQLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALKFYAKLRETSPQSWLYS
PDGGGHTGNGTQRESADELACVLLFLKEFLG

Sequences:

>Translated_671_residues
MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLNNDKARALSDGILNQMQDTRQIPFCQEHRARMYHFHQDAEYPKGV
YRVCTAATYRSGYPEWKILFSVADFDELLGDDVYLGGVSHLVEKPNRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIDKGAMMVNAWRYLDPQGSPIDLIEASDG
FYTKTYLQVSSEGEAKPLNLPNDCDVAGYLAGHLLLTLRKDWHRANQSYPSGALVAVKLNRGEIGAAQLLFAPDETQALE
SVETTKRFVVASLLENVQGRLKAWRFADGKWQEVELPRLPSGALEMTDQPWGGDVVYLAAGDFTTPLTLFALDLNVMELT
VMRRQPQQFDSDGIEVRQFWTTSADGERIPYFHVGKNAAPDTPTLVYAYGGFGIPELPHYLGSIGKYWLEEGNAFVLANI
RGGGEFGPRWHQAAQGISKHKSVDDLLAVVRDLSERGMSLPEHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMI
RYPLLSAGASWTDEYGNPQKYEVCKRQLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALKFYAKLRETSPQSWLYS
PDGGGHTGNGTQRESADELACVLLFLKEFLG
>Mature_671_residues
MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLNNDKARALSDGILNQMQDTRQIPFCQEHRARMYHFHQDAEYPKGV
YRVCTAATYRSGYPEWKILFSVADFDELLGDDVYLGGVSHLVEKPNRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIDKGAMMVNAWRYLDPQGSPIDLIEASDG
FYTKTYLQVSSEGEAKPLNLPNDCDVAGYLAGHLLLTLRKDWHRANQSYPSGALVAVKLNRGEIGAAQLLFAPDETQALE
SVETTKRFVVASLLENVQGRLKAWRFADGKWQEVELPRLPSGALEMTDQPWGGDVVYLAAGDFTTPLTLFALDLNVMELT
VMRRQPQQFDSDGIEVRQFWTTSADGERIPYFHVGKNAAPDTPTLVYAYGGFGIPELPHYLGSIGKYWLEEGNAFVLANI
RGGGEFGPRWHQAAQGISKHKSVDDLLAVVRDLSERGMSLPEHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMI
RYPLLSAGASWTDEYGNPQKYEVCKRQLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALKFYAKLRETSPQSWLYS
PDGGGHTGNGTQRESADELACVLLFLKEFLG

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=408, Percent_Identity=27.4509803921569, Blast_Score=150, Evalue=5e-36,
Organism=Homo sapiens, GI284172420, Length=275, Percent_Identity=25.8181818181818, Blast_Score=81, Evalue=4e-15,
Organism=Homo sapiens, GI284172413, Length=275, Percent_Identity=25.8181818181818, Blast_Score=81, Evalue=4e-15,
Organism=Homo sapiens, GI70778815, Length=275, Percent_Identity=25.8181818181818, Blast_Score=81, Evalue=4e-15,
Organism=Homo sapiens, GI284172438, Length=275, Percent_Identity=25.8181818181818, Blast_Score=81, Evalue=4e-15,
Organism=Homo sapiens, GI284172431, Length=275, Percent_Identity=25.8181818181818, Blast_Score=81, Evalue=4e-15,
Organism=Homo sapiens, GI108860686, Length=275, Percent_Identity=25.8181818181818, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI108860692, Length=266, Percent_Identity=25.9398496240602, Blast_Score=80, Evalue=9e-15,
Organism=Escherichia coli, GI1788150, Length=299, Percent_Identity=29.4314381270903, Blast_Score=107, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24583414, Length=414, Percent_Identity=27.536231884058, Blast_Score=155, Evalue=1e-37,
Organism=Drosophila melanogaster, GI221510989, Length=268, Percent_Identity=32.089552238806, Blast_Score=140, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 74660; Mature: 74660

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLNNDKARALSDGILNQMQDTRQIPFC
CCCCCCHHHHHHCCCCHHHHCHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCH
QEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSVADFDELLGDDVYLGGVSH
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHCCCEEECCHHH
LVEKPNRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFPAGKNHVSWRDENSVWVCPAW
HHCCCCCEEEEEECCCCCEEEEEEEECCCCCEECCCCCCCCCCCCCCCCCCCCEEEECCC
DERQLTESGYPREVWLVERGKSFEESLPVYQIDKGAMMVNAWRYLDPQGSPIDLIEASDG
CCHHHHHCCCCCEEEEEECCCCHHHCCCEEEECCCCEEEEEEEEECCCCCCEEEEECCCC
FYTKTYLQVSSEGEAKPLNLPNDCDVAGYLAGHLLLTLRKDWHRANQSYPSGALVAVKLN
EEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEC
RGEIGAAQLLFAPDETQALESVETTKRFVVASLLENVQGRLKAWRFADGKWQEVELPRLP
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEECCCCC
SGALEMTDQPWGGDVVYLAAGDFTTPLTLFALDLNVMELTVMRRQPQQFDSDGIEVRQFW
CCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCHHHHHHHHHCCCHHCCCCCCEEEEEE
TTSADGERIPYFHVGKNAAPDTPTLVYAYGGFGIPELPHYLGSIGKYWLEEGNAFVLANI
ECCCCCCCCCEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEE
RGGGEFGPRWHQAAQGISKHKSVDDLLAVVRDLSERGMSLPEHIGLQGGSNGGLITAAAF
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCEEEHHHH
VREPQSIGALVCEVPLTDMIRYPLLSAGASWTDEYGNPQKYEVCKRQLGELSPYHNLSDG
HHCCHHHCEEEEECCHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHCCCCCCCCCCC
IDYPPALITTSLSDDRVHPAHALKFYAKLRETSPQSWLYSPDGGGHTGNGTQRESADELA
CCCCCHHEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCHHHHH
CVLLFLKEFLG
HHHHHHHHHHC
>Mature Secondary Structure
MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLNNDKARALSDGILNQMQDTRQIPFC
CCCCCCHHHHHHCCCCHHHHCHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCH
QEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSVADFDELLGDDVYLGGVSH
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHCCCEEECCHHH
LVEKPNRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFPAGKNHVSWRDENSVWVCPAW
HHCCCCCEEEEEECCCCCEEEEEEEECCCCCEECCCCCCCCCCCCCCCCCCCCEEEECCC
DERQLTESGYPREVWLVERGKSFEESLPVYQIDKGAMMVNAWRYLDPQGSPIDLIEASDG
CCHHHHHCCCCCEEEEEECCCCHHHCCCEEEECCCCEEEEEEEEECCCCCCEEEEECCCC
FYTKTYLQVSSEGEAKPLNLPNDCDVAGYLAGHLLLTLRKDWHRANQSYPSGALVAVKLN
EEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEC
RGEIGAAQLLFAPDETQALESVETTKRFVVASLLENVQGRLKAWRFADGKWQEVELPRLP
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEECCCCC
SGALEMTDQPWGGDVVYLAAGDFTTPLTLFALDLNVMELTVMRRQPQQFDSDGIEVRQFW
CCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCHHHHHHHHHCCCHHCCCCCCEEEEEE
TTSADGERIPYFHVGKNAAPDTPTLVYAYGGFGIPELPHYLGSIGKYWLEEGNAFVLANI
ECCCCCCCCCEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEE
RGGGEFGPRWHQAAQGISKHKSVDDLLAVVRDLSERGMSLPEHIGLQGGSNGGLITAAAF
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCEEEHHHH
VREPQSIGALVCEVPLTDMIRYPLLSAGASWTDEYGNPQKYEVCKRQLGELSPYHNLSDG
HHCCHHHCEEEEECCHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHCCCCCCCCCCC
IDYPPALITTSLSDDRVHPAHALKFYAKLRETSPQSWLYSPDGGGHTGNGTQRESADELA
CCCCCHHEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCHHHHH
CVLLFLKEFLG
HHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.23 {tosyl-Arg} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 80 {acetyl-tyrosine} 0.47 {tosyl-Lys-methyl} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]