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The map label for this gene is rsmE [H]

Identifier: 161869288

GI number: 161869288

Start: 300855

End: 301580

Strand: Reverse

Name: rsmE [H]

Synonym: NMCC_0293

Alternate gene names: 161869288

Gene position: 301580-300855 (Counterclockwise)

Preceding gene: 161869290

Following gene: 161869270

Centisome position: 14.0

GC content: 59.23

Gene sequence:

>726_bases
ATGCCCCGATTCCACCTGCCCGAAAACCTTTCCGTCGGACAAACCGTCGCCCTGCCCGACAACATCGTCCGCCACCTCAA
CGTCCTGCGCGTCCGCCCCAACGAAAACATCACCCTCTTCGACGGCAAAGGCAAGGCACACGCCGCACGGCTGACCGTTT
TGGAAAAACGCCGCGCCGAAGCCGAAATCCTGCACGAAGACACAACCGACAACGAGTCCCCGCTCAACATCACGCTCATC
CAATCCATATCTTCCGGCGAACGTATGGACTTTACCCTGCAAAAAAGCGTCGAACTCGGCGTAACCGCCATACAGCCCGT
CATCAGCGAACGCTGCATCGTCCGCCTCGATGGGGAACGCGCCGCCAAACGCCTCGCACGCTGGCAGGAAATCGTCATCT
CCGCGTGCGAACAAAGCGGCAGGAACACCGTTCCCCCCGTACTGCCCATCATCGGCTACCGTGAAGCACTCGACAAAATG
CCGTCTGAAAGCACCAAGCTGATTATGAGCATCAACCGCGCCCGCAAACTCGGCGACATACGCCAACCGTCCGGCGCAAT
CGTCTTTATGGTCGGGCCCGAAGGCGGCTGGACAGAACAGGAAGAACAACAGGCATTTGAAGCTGGCTTTCAGGCGGTTA
CACTCGGCAAACGGATTTTACGCACAGAAACCGCCCCACTCGCCGCCCTCGCCGCCATGCAGACGCTTTGGGGCGATTTC
GCATAA

Upstream 100 bases:

>100_bases
TACTGTCCTCATATTTTTGAACTTGCGGCGCGCGAACGTATAATGTCCGCTTCCATCACGCCGCTGCGACGGATTATAAC
CGTCCGAACCGCCAAAAACT

Downstream 100 bases:

>100_bases
ACAGAAATGCCGTCTGAAACCCGTTCAGACGGCATTTTGCAGCCGATTAAGATAGTAGGTTCAAATAAGATTTCCCGTGT
CGTCATTCCCGCGAAAGCGG

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MPRFHLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKGKAHAARLTVLEKRRAEAEILHEDTTDNESPLNITLI
QSISSGERMDFTLQKSVELGVTAIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
PSESTKLIMSINRARKLGDIRQPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKRILRTETAPLAALAAMQTLWGDF
A

Sequences:

>Translated_241_residues
MPRFHLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKGKAHAARLTVLEKRRAEAEILHEDTTDNESPLNITLI
QSISSGERMDFTLQKSVELGVTAIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
PSESTKLIMSINRARKLGDIRQPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKRILRTETAPLAALAAMQTLWGDF
A
>Mature_240_residues
PRFHLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKGKAHAARLTVLEKRRAEAEILHEDTTDNESPLNITLIQ
SISSGERMDFTLQKSVELGVTAIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKMP
SESTKLIMSINRARKLGDIRQPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKRILRTETAPLAALAAMQTLWGDFA

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=242, Percent_Identity=39.6694214876033, Blast_Score=177, Evalue=8e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26699; Mature: 26568

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRFHLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKGKAHAARLTVLEKRRAE
CCCCCCCCCCCCCCEEECHHHHHHHHHEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHH
AEILHEDTTDNESPLNITLIQSISSGERMDFTLQKSVELGVTAIQPVISERCIVRLDGER
HHHHHCCCCCCCCCEEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHCCEEEEECCHH
AAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKMPSESTKLIMSINRARKLGDI
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHCCC
RQPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKRILRTETAPLAALAAMQTLWGDF
CCCCCCEEEEECCCCCCCCHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
A
C
>Mature Secondary Structure 
PRFHLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKGKAHAARLTVLEKRRAE
CCCCCCCCCCCCCEEECHHHHHHHHHEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHH
AEILHEDTTDNESPLNITLIQSISSGERMDFTLQKSVELGVTAIQPVISERCIVRLDGER
HHHHHCCCCCCCCCEEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHCCEEEEECCHH
AAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKMPSESTKLIMSINRARKLGDI
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHCCC
RQPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKRILRTETAPLAALAAMQTLWGDF
CCCCCCEEEEECCCCCCCCHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]