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The map label for this gene is ptsI [H]

Identifier: 161869148

GI number: 161869148

Start: 141752

End: 143527

Strand: Direct

Name: ptsI [H]

Synonym: NMCC_0142

Alternate gene names: 161869148

Gene position: 141752-143527 (Clockwise)

Preceding gene: 161869147

Following gene: 161869152

Centisome position: 6.58

GC content: 57.38

Gene sequence:

>1776_bases
ATGAGTATCGTGCTGCACGGCGTGGCGGCGGGCAAAGGTATTGCCGTCGGTTGCGCCCACCTGATTGCGCGCGGTACGGA
GGAAGTGCCGCAGTATGATGTTGCGGAGGCGGACACCGATGCCGAAGCCGAACGTTTCGATGCCGCCGTCAAAGCCACGC
GCAAAGAGTTGGAACAGCTCCGCAGCGCGATTCCCGAAAACGCCCCGACCGAGTTGGGCGCGTTCATCTCGCTGCACCTG
ATGCTCTTGACCGATGTTACCTTGTCGCGCGAACCCGTCGATATTTTAAGGGAACAAAAAATCAACGCCGAGTGGGCATT
GAAGCAGCAGAGCGACAAACTCGCTGCCCAATTCGACAATATGGACGATGCCTATTTGCGCGAACGCAAGCAGGATATGC
TGCAAGTCGTCCGCCGCATCCACAACAACCTGATCGGGCAGGGCAACGAGTTGGAAGTTGCCGACAACCTGTTTGACGAA
ACCGTTCTGATTGCAAACGACCTTTCGCCCGCCGACACGGTTTTGTTTAAAGAGCAGCGCATTGCCGCCTTCGTTACCGA
TGCCGGCGGCCCCACCGGGCATACGGCGATTTTGGGCAGGAGCTTGGACATCCCGTCCGTCGTCGGGCTGCACAACGCGC
GCAAACTGATTACCGAGGGCGAAACGGTCATTGTGGACGGTATCAACGGCGTGTTGATTATCGCGCCGGATGAGTCGGTG
TTGAACGAATACCGCCGCCGTGCCCGCGAATACCGCAGCCACAAACGCGATTTGAACAAGCTCAAAAAAACCGCCGCCGC
CACCGCTGACGGGGTCTGCATCGAGCTTGTGGGCAATATAGAATCCGCCGAAGACGTGAAACCGCTGCACAACCTCGGCG
CAGACGGCATCGGGCTGTTCCGCAGCGAGTTTCTTTACCTGAACCGCGATACGATGCCGTCTGAAGACGAGCAGTACGAA
GTGTACAGCGCGATTGTCAAAAAAATGAAGGGCAAAAGCGTAACGATACGGACAGTCGATTTGGGCGTGGATAAAAACCC
CCGCTGGTTCGGGAAAAACAGCACGCCCAACGGCAGCCTCAACCCCGCGCTGGGCATGACCGGCATCCGCCTGTGCCTTG
CCGAACCGGTCATGTTCCGCACCCAGATGCGCGCCATCCTCCGTGCGGCGGTACACGGCCCTGTGCGGATGATGTGGCCG
ATGATTACCTCCGTATCCGAAGTGCGCCAGTGCCTGATTCATTTGGACACCGCCCGCCGCCAGCTTGCCGAACGCGGCGA
TGCCTTCGGTAAAGTCGGCATCGGCTGTATGATTGAAATTCCGTCTGCCGCGCTGACCGTCGGCAGTATTTTGAAACTGG
TCGATTTCATCTCCGTCGGTACCAACGACCTGATTCAATACATCTTGTCCGTCGATCGCGGCGACGACAGCGTCAGCCAT
CTCTACCAGCCCGGACATCCTTCCGTGTTGAAAATGCTGCAACACGTCATCCGTACCGCCAACCGCATGGACAAAGACGT
ATCCGTATGCGGCGAGATGGCGGGCGATACGGCGTTTACCCGCATCCTGCTTGGTATGGGGCTGCGCCGTTTTTCCATGA
ATCCGAACAACATCCTGCCCGTCAAAAACATCATCCTGCACAGCAATATCGCACAGCTCGAAAACGAGATTGCCAAAATC
GTCCGCTGCGAGGACGAGGAAAAGGCGGAAAAGCTGATCAAACAGATGAACAGCGTGTCTGTCGAGGAAGAAGCCGACTT
CAAGGGGCGGAAATAA

Upstream 100 bases:

>100_bases
AGGGTACGGTCATCGAGCTGGAGACGGACGGCGCGGACGAGGCGGAAGCGATGCGCGCCCTGACCGACTTAATCAACGAC
TACTTCGGCGAGGGCGAATA

Downstream 100 bases:

>100_bases
ATACGGCAGGTAAAAAATAGAAATACTTAACAATGCCCGCAATCTGAAATTTTGCCATTCTTGCAAAATAGAAAACCGAA
ACAGAAACCCAAAATCGGTC

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I; Protein I [H]

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAERFDAAVKATRKELEQLRSAIPENAPTELGAFISLHL
MLLTDVTLSREPVDILREQKINAEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLITEGETVIVDGINGVLIIAPDESV
LNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIELVGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYE
VYSAIVKKMKGKSVTIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVHGPVRMMWP
MITSVSEVRQCLIHLDTARRQLAERGDAFGKVGIGCMIEIPSAALTVGSILKLVDFISVGTNDLIQYILSVDRGDDSVSH
LYQPGHPSVLKMLQHVIRTANRMDKDVSVCGEMAGDTAFTRILLGMGLRRFSMNPNNILPVKNIILHSNIAQLENEIAKI
VRCEDEEKAEKLIKQMNSVSVEEEADFKGRK

Sequences:

>Translated_591_residues
MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAERFDAAVKATRKELEQLRSAIPENAPTELGAFISLHL
MLLTDVTLSREPVDILREQKINAEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLITEGETVIVDGINGVLIIAPDESV
LNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIELVGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYE
VYSAIVKKMKGKSVTIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVHGPVRMMWP
MITSVSEVRQCLIHLDTARRQLAERGDAFGKVGIGCMIEIPSAALTVGSILKLVDFISVGTNDLIQYILSVDRGDDSVSH
LYQPGHPSVLKMLQHVIRTANRMDKDVSVCGEMAGDTAFTRILLGMGLRRFSMNPNNILPVKNIILHSNIAQLENEIAKI
VRCEDEEKAEKLIKQMNSVSVEEEADFKGRK
>Mature_590_residues
SIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAERFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLM
LLTDVTLSREPVDILREQKINAEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDET
VLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLITEGETVIVDGINGVLIIAPDESVL
NEYRRRAREYRSHKRDLNKLKKTAAATADGVCIELVGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEV
YSAIVKKMKGKSVTIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVHGPVRMMWPM
ITSVSEVRQCLIHLDTARRQLAERGDAFGKVGIGCMIEIPSAALTVGSILKLVDFISVGTNDLIQYILSVDRGDDSVSHL
YQPGHPSVLKMLQHVIRTANRMDKDVSVCGEMAGDTAFTRILLGMGLRRFSMNPNNILPVKNIILHSNIAQLENEIAKIV
RCEDEEKAEKLIKQMNSVSVEEEADFKGRK

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=552, Percent_Identity=36.0507246376812, Blast_Score=355, Evalue=5e-99,
Organism=Escherichia coli, GI1789193, Length=562, Percent_Identity=32.9181494661922, Blast_Score=268, Evalue=7e-73,
Organism=Escherichia coli, GI48994992, Length=472, Percent_Identity=28.8135593220339, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1788726, Length=580, Percent_Identity=29.6551724137931, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1787994, Length=441, Percent_Identity=26.530612244898, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 65228; Mature: 65097

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAERFDAAVKATRKELEQL
CCEEEEECCCCCCHHHHHHHHHHCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKINAEWALKQQSDKLAAQFDN
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
MDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDETVLIANDLSPADTVLFKEQR
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCEEEEECCCCCHHHHHHHHHH
IAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLITEGETVIVDGINGVLIIAPDESV
HEEEEECCCCCCCCHHHEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCHHH
LNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIELVGNIESAEDVKPLHNLGADGIGLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCCHHHH
RSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSVTIRTVDLGVDKNPRWFGKNSTPNGSL
HHHEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCCC
NPALGMTGIRLCLAEPVMFRTQMRAILRAAVHGPVRMMWPMITSVSEVRQCLIHLDTARR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QLAERGDAFGKVGIGCMIEIPSAALTVGSILKLVDFISVGTNDLIQYILSVDRGDDSVSH
HHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHH
LYQPGHPSVLKMLQHVIRTANRMDKDVSVCGEMAGDTAFTRILLGMGLRRFSMNPNNILP
CCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHCHHHCCCCCCCCCC
VKNIILHSNIAQLENEIAKIVRCEDEEKAEKLIKQMNSVSVEEEADFKGRK
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAERFDAAVKATRKELEQL
CEEEEECCCCCCHHHHHHHHHHCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKINAEWALKQQSDKLAAQFDN
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
MDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDETVLIANDLSPADTVLFKEQR
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCEEEEECCCCCHHHHHHHHHH
IAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLITEGETVIVDGINGVLIIAPDESV
HEEEEECCCCCCCCHHHEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCHHH
LNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIELVGNIESAEDVKPLHNLGADGIGLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCCHHHH
RSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSVTIRTVDLGVDKNPRWFGKNSTPNGSL
HHHEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCCC
NPALGMTGIRLCLAEPVMFRTQMRAILRAAVHGPVRMMWPMITSVSEVRQCLIHLDTARR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QLAERGDAFGKVGIGCMIEIPSAALTVGSILKLVDFISVGTNDLIQYILSVDRGDDSVSH
HHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHH
LYQPGHPSVLKMLQHVIRTANRMDKDVSVCGEMAGDTAFTRILLGMGLRRFSMNPNNILP
CCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHCHHHCCCCCCCCCC
VKNIILHSNIAQLENEIAKIVRCEDEEKAEKLIKQMNSVSVEEEADFKGRK
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1653223 [H]