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The map label for this gene is thiC [H]

Identifier: 161869152

GI number: 161869152

Start: 147223

End: 149124

Strand: Direct

Name: thiC [H]

Synonym: NMCC_0147

Alternate gene names: 161869152

Gene position: 147223-149124 (Clockwise)

Preceding gene: 161869148

Following gene: 161869172

Centisome position: 6.84

GC content: 57.57

Gene sequence:

>1902_bases
ATGACTACGCCAAAAAAAACTGCCAAAACTTCCGGCAACGAAGCACGCGAGCTTGCCGACTTGAGCGAAGACATCGGCAT
CCGCTTCAAATATCCGAACTCGGAACGCGTGTATCTGCAAGGCAGCCGCGACGACATCCGCGTGCCTTTGCGCGAAATCC
GCCAGGACGACACCTACACGGCGCAAGGCACGGAAGCCAATCCGCCGATTCCCGTCTATGACACCAGCGGCGTGTACGGC
GATCCGGCGGCACACATCGATTTAAAACAAGGGCTGCCCCACATCCGCACGGCGTGGCTGGACGAACGCGGCGATACCGA
AATCCTGCCCAAACTCTCCAGCGAATACGGCATCGAACGCGCGCACGACCCGAAAACCGCCCATCTGCGTTTCAACCAAA
TCACCCGCCCGCGCCGCGCCAAAGCCGGACGCAACGTAACCCAATTACATTATGCCCGGCAAGGCATTATCACGCCCGAA
ATGGAGTTTGTCGCCATACGCGAACGTTTAAAATTAGACGAATTGTCCCAAAAACCGGAATACGCCAAGCTCTTGAAACA
ACACGCAGGGCAAAGTTTCGGCGCGAATATCCCGACCCATCCCGACCAAATCACGCCTGAATTCGTGCGCCGAGAAATCG
CCGCCGGACGCGCGATTATTCCCGCCAACATCAACCACCCCGAACTCGAACCGATGATTATCGGCCGCAACTTCCGCGTC
AAAATCAACGGCAATTTGGGCAACTCCGCCGTCACCTCCAGCCTGACCGAAGAAGTCGAAAAAATGGTGTGGTCGCTGCG
TTGGGGCGCGGACACGATTATGGATTTGTCCACCGGCGCGCACATCCATGAAACGCGCGAATGGATTATCCGCAACGCGC
CCGTCCCCATCGGCACCGTGCCGATTTACCAAGCGTTGGAAAAAACCGGCGGCATCGCCGAAGATTTGACTTGGGATTTG
TTCCGCGACACCCTCATCGAACAGGCGGAACAAGGCGTGGACTATTTCACCATACACGCGGGCGTGTTGCTGCGTTATGT
ACCGATGACCGCCAACCGCCTCACCGGCATCGTCTCGCGCGGCGGTTCGATTATGGCGAAATGGTGTTTGGCACATCATC
GGGAAAACTTCCTCTACACGCATTTCGACGAAATCTGCGAAATTATGAAAGCGTATGACGTATCGTTCAGCCTCGGCGAC
GGCCTGCGCCCCGGCTGCATTGCCGATGCCAACGACGAATCCCAATTCGCCGAGCTGCACACCTTGGGCGAATTGACCTC
CAAAGCGTGGAAACACGACGTACAGGTGATGATCGAAGGCCCCGGCCATGTACCGCTGCAACGCGTCAAAGAAAATATGA
CCGAAGAGTTGCAACACTGTTTTGAAGCACCGTTTTACACCCTCGGCCCGCTCGTTACCGACATCGCCCCCGGCTACGAC
CACATCACCTCGGGCATAGGCGCTGCCAATATCGGCTGGTACGGCACGGCGATGCTCTGTTACGTTACCCCCAAAGAACA
TTTGGGACTGCCCGACAAAGAAGACGTGCGTACCGGCATCATCACCTACAAACTCGCCGCCCACGCCGCCGACCTCGCCA
AAGGCTGGCCGGGCGCACAGTTGCGCGACAACGCCCTGAGCAAGGCGCGTTTCGAGTTCCGCTGGCGCGACCAGTTCCGC
CTAAGCCTCGACCCCGAACGCGCCGAAAGCTTCCACGACGAAACCCTGCCCGCCGAAGGCGCGAAAATCGCCCACTTCTG
TTCGATGTGCGGCCCCAAATTCTGCTCGATGAAAATCACGCAGGAAGTGCGCGACTACGCCGACAAGCAAAAAGCCCAAC
GGCAGGGCATGGAGGAAAAAGCGGTCGAGTTCGTCAAAAAAGGCGCGAAGATTTACAGTTGA

Upstream 100 bases:

>100_bases
GATTCTGCCTTTCAGACGGCATTTTTTGATACTCGGGCCGTTTGAAAAAGCAAAGTGCTCCTGTTTCTTTATTCTAAACG
AGAAAACAGGAGCATTTTTT

Downstream 100 bases:

>100_bases
AACGTCAAGCAAAAAATGCCGTCTGAAACCCGGAAAAAAGGCTTCAGACGGCATTCTTTCGCTTGTGAAAATATAGTGGA
TTAACAAAAATCAGGACAAG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 633; Mature: 632

Protein sequence:

>633_residues
MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRDDIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGVYG
DPAAHIDLKQGLPHIRTAWLDERGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKAGRNVTQLHYARQGIITPE
MEFVAIRERLKLDELSQKPEYAKLLKQHAGQSFGANIPTHPDQITPEFVRREIAAGRAIIPANINHPELEPMIIGRNFRV
KINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDLSTGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDL
FRDTLIEQAEQGVDYFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAYDVSFSLGD
GLRPGCIADANDESQFAELHTLGELTSKAWKHDVQVMIEGPGHVPLQRVKENMTEELQHCFEAPFYTLGPLVTDIAPGYD
HITSGIGAANIGWYGTAMLCYVTPKEHLGLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFR
LSLDPERAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEFVKKGAKIYS

Sequences:

>Translated_633_residues
MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRDDIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGVYG
DPAAHIDLKQGLPHIRTAWLDERGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKAGRNVTQLHYARQGIITPE
MEFVAIRERLKLDELSQKPEYAKLLKQHAGQSFGANIPTHPDQITPEFVRREIAAGRAIIPANINHPELEPMIIGRNFRV
KINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDLSTGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDL
FRDTLIEQAEQGVDYFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAYDVSFSLGD
GLRPGCIADANDESQFAELHTLGELTSKAWKHDVQVMIEGPGHVPLQRVKENMTEELQHCFEAPFYTLGPLVTDIAPGYD
HITSGIGAANIGWYGTAMLCYVTPKEHLGLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFR
LSLDPERAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEFVKKGAKIYS
>Mature_632_residues
TTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRDDIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGVYGD
PAAHIDLKQGLPHIRTAWLDERGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKAGRNVTQLHYARQGIITPEM
EFVAIRERLKLDELSQKPEYAKLLKQHAGQSFGANIPTHPDQITPEFVRREIAAGRAIIPANINHPELEPMIIGRNFRVK
INGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDLSTGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLF
RDTLIEQAEQGVDYFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAYDVSFSLGDG
LRPGCIADANDESQFAELHTLGELTSKAWKHDVQVMIEGPGHVPLQRVKENMTEELQHCFEAPFYTLGPLVTDIAPGYDH
ITSGIGAANIGWYGTAMLCYVTPKEHLGLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRL
SLDPERAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEFVKKGAKIYS

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=637, Percent_Identity=67.9748822605965, Blast_Score=892, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 71152; Mature: 71020

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRDDIRVPLREIRQDDTYT
CCCCCCHHCCCCHHHHHHHHHHHCCCEEEECCCCCEEEEECCCCCCCCCHHHCCCCCCEE
AQGTEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLDERGDTEILPKLSSEYGIER
ECCCCCCCCCCEEECCCCCCCCCCEEEHHHCCCHHHHHHHCCCCCCHHHHHHHHHCCCCC
AHDPKTAHLRFNQITRPRRAKAGRNVTQLHYARQGIITPEMEFVAIRERLKLDELSQKPE
CCCCCEEEEEEHHCCCCHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCH
YAKLLKQHAGQSFGANIPTHPDQITPEFVRREIAAGRAIIPANINHPELEPMIIGRNFRV
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCEEEECCEEEE
KINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDLSTGAHIHETREWIIRNAPVPIGTV
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHCCCCCCCCH
PIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVDYFTIHAGVLLRYVPMTANRLTGIVSR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCHHHHHHHHHHC
GGSIMAKWCLAHHRENFLYTHFDEICEIMKAYDVSFSLGDGLRPGCIADANDESQFAELH
CCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHHH
TLGELTSKAWKHDVQVMIEGPGHVPLQRVKENMTEELQHCFEAPFYTLGPLVTDIAPGYD
HHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHH
HITSGIGAANIGWYGTAMLCYVTPKEHLGLPDKEDVRTGIITYKLAAHAADLAKGWPGAQ
HHHCCCCCCCCCCEEEEEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LRDNALSKARFEFRWRDQFRLSLDPERAESFHDETLPAEGAKIAHFCSMCGPKFCSMKIT
HHHHHHHHHHEEEEECCCEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHH
QEVRDYADKQKAQRQGMEEKAVEFVKKGAKIYS
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRDDIRVPLREIRQDDTYT
CCCCCHHCCCCHHHHHHHHHHHCCCEEEECCCCCEEEEECCCCCCCCCHHHCCCCCCEE
AQGTEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLDERGDTEILPKLSSEYGIER
ECCCCCCCCCCEEECCCCCCCCCCEEEHHHCCCHHHHHHHCCCCCCHHHHHHHHHCCCCC
AHDPKTAHLRFNQITRPRRAKAGRNVTQLHYARQGIITPEMEFVAIRERLKLDELSQKPE
CCCCCEEEEEEHHCCCCHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCH
YAKLLKQHAGQSFGANIPTHPDQITPEFVRREIAAGRAIIPANINHPELEPMIIGRNFRV
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCEEEECCEEEE
KINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDLSTGAHIHETREWIIRNAPVPIGTV
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHCCCCCCCCH
PIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVDYFTIHAGVLLRYVPMTANRLTGIVSR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCHHHHHHHHHHC
GGSIMAKWCLAHHRENFLYTHFDEICEIMKAYDVSFSLGDGLRPGCIADANDESQFAELH
CCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHHH
TLGELTSKAWKHDVQVMIEGPGHVPLQRVKENMTEELQHCFEAPFYTLGPLVTDIAPGYD
HHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHH
HITSGIGAANIGWYGTAMLCYVTPKEHLGLPDKEDVRTGIITYKLAAHAADLAKGWPGAQ
HHHCCCCCCCCCCEEEEEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LRDNALSKARFEFRWRDQFRLSLDPERAESFHDETLPAEGAKIAHFCSMCGPKFCSMKIT
HHHHHHHHHHEEEEECCCEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHH
QEVRDYADKQKAQRQGMEEKAVEFVKKGAKIYS
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA