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The map label for this gene is thiG [H]

Identifier: 161869120

GI number: 161869120

Start: 114789

End: 115577

Strand: Reverse

Name: thiG [H]

Synonym: NMCC_0114

Alternate gene names: 161869120

Gene position: 115577-114789 (Counterclockwise)

Preceding gene: 161869121

Following gene: 161869116

Centisome position: 5.37

GC content: 61.85

Gene sequence:

>789_bases
ATGCTCACCCTGTACAGCGAAACTTTCCCTTCGCGGCTGCTGCTCGGCACAGCCGCCTACCCGACCCCTGAAATCCTCAA
ACAATCCGTCCGAACCGCCCGGCCCGCGATGATTACCGTCTCGCTGCGCCGCGCGGGATGCGGCGGCGAGGCGCACGGTC
AGGGGTTTTGGTCGCTGCTTCAAGAAACCGGCGTTCCCGTCCTGCCGAACACGGCAGGCTGCCAAAGCGTGCAGGAAGCG
GTAACGACGGCGCAAATGGCGCGCGAAGTGTTTGAAACCGATTGGATTAAACTCGAACTCATCGGCGACGACGACACCTT
GCAGCCGGATGTGTTCCAACTTGTCGAAGCGGCGGAAATCCTGATTAAAGACGGCTTCAAAGTGCTGCCTTATTGCACCG
AAGACCTGATTGCCTGCCGCCGCCTGCTCGATGTGGGCTGTCAGGCGTTGATGCCGTGGGCGGCCCCGATCGGCACGGGT
TTGGGCGCGGTTCACGCCTACGCGTTGAACGTCCTGCGCGAACGCCTGCCCTACACGCCGCTGATTATCGACGCGGGCTT
GGGTTTGCCCTCACAGGCGGCACAAGTGATGGAATGGGGCTTTGACGGCGTGCTTTTGAACACCGCCGTTTCCCGCAGCG
GCGATCCAGTCAATATGGCACGCGCCTTCGCACTCGCCGTCGAATCCGGACGGCTGGCATTTGAAGCCGGACCGGTCGAA
GCACGCGACAAAGCGCAAGCCAGCACGCCGACAGTCGGACAGCCGTTTTGGCATTCGGCGGAATATTGA

Upstream 100 bases:

>100_bases
TCACTTAGTGCTTCAGCACCTTAGAGAATCGTTTTCTTTGAGCTAAGACGAGGCAACGCCGTACTGGTTTTTGTTAATCC
ACTATACAAAGGAACCCATT

Downstream 100 bases:

>100_bases
AAAAGGCGGCAAAAATGCCGTCTGAAGGCTTCAGACGGCATCGCGGCTCAAAACGGCGGCGGTCCGAAACGGACAAACCG
CCATTCCCCGGCATCACGGC

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MLTLYSETFPSRLLLGTAAYPTPEILKQSVRTARPAMITVSLRRAGCGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEA
VTTAQMAREVFETDWIKLELIGDDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDVGCQALMPWAAPIGTG
LGAVHAYALNVLRERLPYTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVNMARAFALAVESGRLAFEAGPVE
ARDKAQASTPTVGQPFWHSAEY

Sequences:

>Translated_262_residues
MLTLYSETFPSRLLLGTAAYPTPEILKQSVRTARPAMITVSLRRAGCGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEA
VTTAQMAREVFETDWIKLELIGDDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDVGCQALMPWAAPIGTG
LGAVHAYALNVLRERLPYTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVNMARAFALAVESGRLAFEAGPVE
ARDKAQASTPTVGQPFWHSAEY
>Mature_262_residues
MLTLYSETFPSRLLLGTAAYPTPEILKQSVRTARPAMITVSLRRAGCGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEA
VTTAQMAREVFETDWIKLELIGDDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDVGCQALMPWAAPIGTG
LGAVHAYALNVLRERLPYTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVNMARAFALAVESGRLAFEAGPVE
ARDKAQASTPTVGQPFWHSAEY

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=253, Percent_Identity=39.9209486166008, Blast_Score=198, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 28232; Mature: 28232

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLYSETFPSRLLLGTAAYPTPEILKQSVRTARPAMITVSLRRAGCGGEAHGQGFWSLL
CEEECCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHH
QETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIGDDDTLQPDVFQLVEAAEI
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHH
LIKDGFKVLPYCTEDLIACRRLLDVGCQALMPWAAPIGTGLGAVHAYALNVLRERLPYTP
HHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
LIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVNMARAFALAVESGRLAFEAGPVE
EEEECCCCCCHHHHHHHHHCCCCEEEHHHHHCCCCCHHHHHHHHHHHHCCCEEEECCCCC
ARDKAQASTPTVGQPFWHSAEY
CHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLTLYSETFPSRLLLGTAAYPTPEILKQSVRTARPAMITVSLRRAGCGGEAHGQGFWSLL
CEEECCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHH
QETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIGDDDTLQPDVFQLVEAAEI
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHH
LIKDGFKVLPYCTEDLIACRRLLDVGCQALMPWAAPIGTGLGAVHAYALNVLRERLPYTP
HHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
LIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVNMARAFALAVESGRLAFEAGPVE
EEEECCCCCCHHHHHHHHHCCCCEEEHHHHHCCCCCHHHHHHHHHHHHCCCEEEECCCCC
ARDKAQASTPTVGQPFWHSAEY
CHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA