Click here to switch to the map view.

The map label for this gene is folD [H]

Identifier: 161869116

GI number: 161869116

Start: 109313

End: 110071

Strand: Reverse

Name: folD [H]

Synonym: NMCC_0107

Alternate gene names: 161869116

Gene position: 110071-109313 (Counterclockwise)

Preceding gene: 161869120

Following gene: 161869114

Centisome position: 5.11

GC content: 58.37

Gene sequence:

>759_bases
ATGCCTTGCCTGGCCGTGGTTTTGGTCGGAGGCGACCCTGCCAGCGCGGTTTATGTCCGCAACAAGAAAACTGCCTGCCA
AAAATGCGGCATCAAATCACTGTCTTACGAGCTGCCCGAATCAACATCGCAGGAAGAACTGCTGGCACTGGTCGACCGCC
TGAATGCCGATTCCGAAGTGGACGGTATTCTGGTTCAGCTACCGCTGCCGAAGCACCTCGACAGCCAGGCGGTTTTGGAA
CGTATTTCGCCGGATAAGGACGTGGACGGCTTCCATCCTTACAATGTCGGCAGGCTGGCGGTCAAAATGCCGCTGATGCG
CCCGTGTACGCCCAAGGGCGTGATGACGCTTTTGGAAGCTTACGGCATTGATCCGAAGGGGAAAAAAGCGGTCGTGGTCG
GCGCGTCGAATATCGTCGGCCGCCCGCAGGCTTTGGAACTGCTGCTGGCGCGCGCAACGGTAACGGTCTGCCACAGCGCA
ACCGAAAATCTGGCAGACGAGGTTGCCGGAGCCGATATTTTGGTGGTCGGCGTGGGCATTCCGAACTTTGTCAAAGGCGA
ATGGATCAAACCCGGCGCGGTCGTTATCGATGTGGGCATCAACCGTTTGGACGACGGCAGCCTGTGCGGCGACGTGGAAT
TTGAAACGGCAAAAGAACGGGCGGCGATGATTACGCCCGTTCCCGGCGGCGTGGGTCCGATGACGATTGCCACATTGATG
GAAAACACCCTGCACGCGGCTTCACTGCACGATGCTTGA

Upstream 100 bases:

>100_bases
GGATATGTCGGCACAACTGATCAATGGTAAAGAAGTTTCGCAAAAACGCCTGCAGGCGGTTGCCGAAGCGGTGGCGCAAC
GCCAACAGGACAATCTGCAC

Downstream 100 bases:

>100_bases
GCGGTTCTGAAGATAAAAATGCCGTCTGAAAGGCTTTCAGACGGCATTTTGCCGTGTCCGTTTATTTGGGCAGCTTGACG
ACAACCGTATCCGCCAGTAT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MPCLAVVLVGGDPASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLPKHLDSQAVLE
RISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGKKAVVVGASNIVGRPQALELLLARATVTVCHSA
TENLADEVAGADILVVGVGIPNFVKGEWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLM
ENTLHAASLHDA

Sequences:

>Translated_252_residues
MPCLAVVLVGGDPASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLPKHLDSQAVLE
RISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGKKAVVVGASNIVGRPQALELLLARATVTVCHSA
TENLADEVAGADILVVGVGIPNFVKGEWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLM
ENTLHAASLHDA
>Mature_251_residues
PCLAVVLVGGDPASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLPKHLDSQAVLER
ISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGKKAVVVGASNIVGRPQALELLLARATVTVCHSAT
ENLADEVAGADILVVGVGIPNFVKGEWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLME
NTLHAASLHDA

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=256, Percent_Identity=47.265625, Blast_Score=218, Evalue=3e-57,
Organism=Homo sapiens, GI222418558, Length=261, Percent_Identity=46.7432950191571, Blast_Score=210, Evalue=8e-55,
Organism=Homo sapiens, GI94721354, Length=261, Percent_Identity=48.6590038314176, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI36796743, Length=214, Percent_Identity=29.4392523364486, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1786741, Length=251, Percent_Identity=63.7450199203187, Blast_Score=328, Evalue=3e-91,
Organism=Caenorhabditis elegans, GI17568735, Length=256, Percent_Identity=46.09375, Blast_Score=197, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6319558, Length=254, Percent_Identity=49.2125984251969, Blast_Score=235, Evalue=6e-63,
Organism=Saccharomyces cerevisiae, GI6321643, Length=262, Percent_Identity=48.0916030534351, Blast_Score=227, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6322933, Length=290, Percent_Identity=26.8965517241379, Blast_Score=75, Evalue=7e-15,
Organism=Drosophila melanogaster, GI17136816, Length=262, Percent_Identity=50.763358778626, Blast_Score=230, Evalue=8e-61,
Organism=Drosophila melanogaster, GI17136818, Length=262, Percent_Identity=50.763358778626, Blast_Score=229, Evalue=9e-61,
Organism=Drosophila melanogaster, GI62472483, Length=257, Percent_Identity=48.6381322957198, Blast_Score=221, Evalue=3e-58,
Organism=Drosophila melanogaster, GI45551871, Length=257, Percent_Identity=48.6381322957198, Blast_Score=221, Evalue=3e-58,
Organism=Drosophila melanogaster, GI24645718, Length=257, Percent_Identity=48.6381322957198, Blast_Score=221, Evalue=3e-58,
Organism=Drosophila melanogaster, GI17137370, Length=257, Percent_Identity=48.6381322957198, Blast_Score=221, Evalue=3e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 26566; Mature: 26435

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPCLAVVLVGGDPASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEV
CCEEEEEEECCCCCCEEEEECCHHHHHHCCCCHHCCCCCCCCCHHHHHHHHHHCCCCCCC
DGILVQLPLPKHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEA
CCEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHH
YGIDPKGKKAVVVGASNIVGRPQALELLLARATVTVCHSATENLADEVAGADILVVGVGI
HCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
PNFVKGEWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLM
CCCCCCCEECCCCEEEEECCCCCCCCCCCCCCCHHHHHCCCEEEECCCCCCCHHHHHHHH
ENTLHAASLHDA
HHHHHHHHCCCC
>Mature Secondary Structure 
PCLAVVLVGGDPASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEV
CEEEEEEECCCCCCEEEEECCHHHHHHCCCCHHCCCCCCCCCHHHHHHHHHHCCCCCCC
DGILVQLPLPKHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEA
CCEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHH
YGIDPKGKKAVVVGASNIVGRPQALELLLARATVTVCHSATENLADEVAGADILVVGVGI
HCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
PNFVKGEWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLM
CCCCCCCEECCCCEEEEECCCCCCCCCCCCCCCHHHHHCCCEEEECCCCCCCHHHHHHHH
ENTLHAASLHDA
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA