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The map label for this gene is hphIBM [H]

Identifier: 161869095

GI number: 161869095

Start: 84253

End: 84597

Strand: Direct

Name: hphIBM [H]

Synonym: NMCC_0085

Alternate gene names: 161869095

Gene position: 84253-84597 (Clockwise)

Preceding gene: 161869094

Following gene: 161869096

Centisome position: 3.91

GC content: 36.81

Gene sequence:

>345_bases
ATGAATAAGCAATATCCGAAAATTAACTATATCGGTAATAAAGAGAAAATAGCTTCCTGGATTTGTGACCAGCTTCCGTC
TGATGTAGATACAGTTGCAGATGTATTTAGTGGAGGCTGTTCCTTTGCCTACGAAGCCAAAAAACGCGGCTATCGTGTGA
TTACTAACGATATTTTGGCAATTAATTACCAAATTGCTTTAGCATTAATAGAAAACAACCATGAAACATTAAATGACGAT
GATGTCGCAATGATTTTTTCAGGCAGCCCGCATGCCGGTTTTATGAGTCAGCGTTATGCCGAAAAATTCTATTTTCACGA
TGAATACCAACAACTTGATTTGTAA

Upstream 100 bases:

>100_bases
TTTTGCGGCAGCAAAATTGCCCAGCAGCAGCAGGTTGGTAACGCCGTACCGCCTTTATTGGCAAAAGCTATTGCTGAAAG
TATTTTAAAAATGAGTGAAA

Downstream 100 bases:

>100_bases
CGTAAAAATATAGGGAAACTGGATAACCAGTATAAACGCGCTTTGGCGTTTACTTTAATGCGTCGCGCCATGATACGTAA
AATGCCCTATACGGAAGATA

Product: site-specific DNA methylase

Products: NA

Alternate protein names: M.HphIB; Adenine-specific methyltransferase HphIB; M.HphI(A) [H]

Number of amino acids: Translated: 114; Mature: 114

Protein sequence:

>114_residues
MNKQYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAYEAKKRGYRVITNDILAINYQIALALIENNHETLNDD
DVAMIFSGSPHAGFMSQRYAEKFYFHDEYQQLDL

Sequences:

>Translated_114_residues
MNKQYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAYEAKKRGYRVITNDILAINYQIALALIENNHETLNDD
DVAMIFSGSPHAGFMSQRYAEKFYFHDEYQQLDL
>Mature_114_residues
MNKQYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAYEAKKRGYRVITNDILAINYQIALALIENNHETLNDD
DVAMIFSGSPHAGFMSQRYAEKFYFHDEYQQLDL

Specific function: This methylase protects the DNA from cleavage by the HphI endonuclease [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 13054; Mature: 13054

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAYEAKKRGYRVITNDILA
CCCCCCCEEECCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEHHHHCCEEEEECCEEE
INYQIALALIENNHETLNDDDVAMIFSGSPHAGFMSQRYAEKFYFHDEYQQLDL
EEEEEEEEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCHHHCCC
>Mature Secondary Structure
MNKQYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAYEAKKRGYRVITNDILA
CCCCCCCEEECCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEHHHHCCEEEEECCEEE
INYQIALALIENNHETLNDDDVAMIFSGSPHAGFMSQRYAEKFYFHDEYQQLDL
EEEEEEEEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8759008 [H]