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The map label for this gene is pilT [H]

Identifier: 161869069

GI number: 161869069

Start: 52407

End: 53450

Strand: Reverse

Name: pilT [H]

Synonym: NMCC_0052

Alternate gene names: 161869069

Gene position: 53450-52407 (Counterclockwise)

Preceding gene: 161869074

Following gene: 161869068

Centisome position: 2.48

GC content: 57.09

Gene sequence:

>1044_bases
ATGCAGATTACCGACTTACTCGCCTTCGGCGCTAAAAACAAAGCATCCGACCTTCACCTGAGTTCGGGCATATCCCCTAT
GATTCGGGTTCACGGCGATATGCGGCGCATCAACCTTCCCGAAATGAGCGCGGAAGAGGTCGGTAATATGGTAACTTCGG
TAATGAACGACCACCAGCGGAAAATCTACCAGCAAAACTTGGAAGTCGACTTCTCGTTCGAACTGCCCAACGTCGCCCGA
TTCCGCGTCAACGCCTTCAACACCGGCCGCGGTCCCGCCGCCGTATTCCGCACCATTCCCAGCACCGTCTTATCGCTGGA
AGAATTGAAAGCCCCGAGCATTTTCCAAAAAATCGCAGAATCGCCGCGCGGCATGGTATTGGTTACCGGCCCTACCGGTT
CGGGCAAATCGACCACGCTTGCCGCGATGATCAACTACATCAACGAAACCCAGCCGGCACACATCCTGACCATCGAAGAC
CCGATCGAATTCGTCCACCAAAGCAAAAAATCCCTGATTAACCAGCGCGAGCTGCACCAGCACACCCTCAGCTTCGCCAA
CGCGCTGCGTTCCGCATTGCGCGAAGACCCCGACGTTATCCTTGTCGGCGAGATGCGCGACCCCGAAACCATCGGCTTGG
CACTGACCGCCGCCGAAACCGGACACTTGGTTTTCGGCACGCTGCACACGACCGGCGCAGCAAAAACCGTCGACCGTATC
GTGGACGTATTCCCGGCGGGAGAAAAAGAAATGGTGCGCTCTATGCTGTCCGAATCGCTGACCGCCGTCATCTCCCAAAA
CCTGCTGAAAACGCACGACGGCAACGGCCGTGTCGCCTCGCACGAAATCCTGATTGCCAACCCCGCCGTCCGCAACCTCA
TCCGCGAAAACAAAATCACGCAGATTAACTCCGTCCTGCAAACCGGGCAGGCGAGCGGTATGCAGACAATGGACCAATCG
CTGCAATCGCTGGTGCGCCAAGGGCTGATCGCACCGGAAGTCGCACGCAGACGCGCGCAAAACAGCGAAAGTATGAGTTT
CTGA

Upstream 100 bases:

>100_bases
AATGATTAAACAGTTGAAACCCCTCCAGTCAGGGCGGTACAATCAAGGTTGTTAGAACCATTCCAACCAATCGAAACATT
ATACTAAACAGAGCCGCATT

Downstream 100 bases:

>100_bases
CACACAACCGCCTTCCGGCCATACCGGCGGGAAAACAAGGCGCAAACACGCGGGGCGGGACGCAGCATCCCGCCCGGCTA
CCTTTCCGAACAAGAAGCGT

Product: twitching motility protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSAEEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVAR
FRVNAFNTGRGPAAVFRTIPSTVLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAAETGHLVFGTLHTTGAAKTVDRI
VDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNGRVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQS
LQSLVRQGLIAPEVARRRAQNSESMSF

Sequences:

>Translated_347_residues
MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSAEEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVAR
FRVNAFNTGRGPAAVFRTIPSTVLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAAETGHLVFGTLHTTGAAKTVDRI
VDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNGRVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQS
LQSLVRQGLIAPEVARRRAQNSESMSF
>Mature_347_residues
MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSAEEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVAR
FRVNAFNTGRGPAAVFRTIPSTVLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAAETGHLVFGTLHTTGAAKTVDRI
VDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNGRVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQS
LQSLVRQGLIAPEVARRRAQNSESMSF

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=328, Percent_Identity=48.4756097560976, Blast_Score=301, Evalue=3e-83,
Organism=Escherichia coli, GI1786296, Length=375, Percent_Identity=30.1333333333333, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1789723, Length=347, Percent_Identity=31.4121037463977, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38038; Mature: 38038

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSAEEVGNMVTSVMNDHQR
CCHHHHHHHCCCCCCCCCCHHCCCCHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHH
KIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPSTVLSLEELKAPSIFQKIAE
HHHHHCCCEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
SPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIEDPIEFVHQSKKSLINQRELHQ
CCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHH
HTLSFANALRSALREDPDVILVGEMRDPETIGLALTAAETGHLVFGTLHTTGAAKTVDRI
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCEEEEEEECCCHHHHHHHH
VDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNGRVASHEILIANPAVRNLIRENKIT
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECEEEEECHHHHHHHHHCCHH
QINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEVARRRAQNSESMSF
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSAEEVGNMVTSVMNDHQR
CCHHHHHHHCCCCCCCCCCHHCCCCHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHH
KIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPSTVLSLEELKAPSIFQKIAE
HHHHHCCCEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
SPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIEDPIEFVHQSKKSLINQRELHQ
CCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHH
HTLSFANALRSALREDPDVILVGEMRDPETIGLALTAAETGHLVFGTLHTTGAAKTVDRI
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCEEEEEEECCCHHHHHHHH
VDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNGRVASHEILIANPAVRNLIRENKIT
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECEEEEECHHHHHHHHHCCHH
QINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEVARRRAQNSESMSF
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8100347 [H]