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The map label for this gene is pilU [H]

Identifier: 161869068

GI number: 161869068

Start: 51018

End: 52244

Strand: Reverse

Name: pilU [H]

Synonym: NMCC_0051

Alternate gene names: 161869068

Gene position: 52244-51018 (Counterclockwise)

Preceding gene: 161869069

Following gene: 161869067

Centisome position: 2.43

GC content: 55.91

Gene sequence:

>1227_bases
ATGAATACCGATAACCTGCACGACATCTTGGACGAAATGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCGCACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCGCTGACGGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAGTCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACCACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCAGGAATTGGGCCTGCCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCAGCCATATCGTTACCATCGAAGACCCGATTGAGTTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAGTCCCCCGACGTGGTCTGCATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATCGACATCGCCC
TCAACCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAACAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAAGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGATCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
ATTAACGACCTCAACCTCGTTTCCTAA

Upstream 100 bases:

>100_bases
CAGCATCCCGCCCGGCTACCTTTCCGAACAAGAAGCGTCCGCCTTCCTGTTGAAACCTGCCGCCGCAAACTGCAAGGCTT
AAACCGAAAAGAAGCTAACG

Downstream 100 bases:

>100_bases
TTGAAGAAACACCCGATGCCGTCTGAAAACCCCTGCCGTTTTCAGACGGCATCGCTTCATCATCCGCATCCGTGTGCTAC
ACTTCATTTACCTCATAACA

Product: twitching motility protein PilU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
INDLNLVS

Sequences:

>Translated_408_residues
MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
INDLNLVS
>Mature_408_residues
MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
INDLNLVS

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=316, Percent_Identity=31.6455696202532, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1786296, Length=386, Percent_Identity=27.2020725388601, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1789723, Length=278, Percent_Identity=30.2158273381295, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 45733; Mature: 45733

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCETAEAQNASDILISKGF
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
PPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRAN
CCCEEECCCCCCCCHHHCCCCHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCCCCEEECC
AYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRGLLIIVGPTGSGKSTTMATML
CCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHH
EHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
HHHCCCCCCCEEEECCHHHHHHCCHHHEEEHHHCCCCCCCHHHHHHHHHHCCCCEEEECC
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGII
HHCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHH
CQRLALKQDKTGRTAVVDLLINTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFEL
HHHHHHCCCCCCCCEEEHHHHCCCHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
YRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDRINDLNLVS
HHCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCETAEAQNASDILISKGF
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
PPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRAN
CCCEEECCCCCCCCHHHCCCCHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCCCCEEECC
AYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRGLLIIVGPTGSGKSTTMATML
CCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHH
EHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
HHHCCCCCCCEEEECCHHHHHHCCHHHEEEHHHCCCCCCCHHHHHHHHHHCCCCEEEECC
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGII
HHCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHH
CQRLALKQDKTGRTAVVDLLINTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFEL
HHHHHHCCCCCCCCEEEHHHHCCCHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
YRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDRINDLNLVS
HHCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]