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The map label for this gene is def1 [H]

Identifier: 161526293

GI number: 161526293

Start: 3411657

End: 3412202

Strand: Reverse

Name: def1 [H]

Synonym: Bmul_3124

Alternate gene names: 161526293

Gene position: 3412202-3411657 (Counterclockwise)

Preceding gene: 161526297

Following gene: 161526292

Centisome position: 98.95

GC content: 61.54

Gene sequence:

>546_bases
ATGGCTAATGCGGCACACCGCTTCACCGAATACCGAAAGACCATGGCTTTGCTGAACATTCTTCATTACCCCGACAAGCG
GCTGCACAAGGTCGCGAAGCCCGTCGACAAGGTCGACGACCGCATCCGCAAGCTCGTTGCCGACATGGCCGAGACGATGT
ACGCGGCGCCCGGCATCGGCCTCGCCGCGACGCAGGTCGACGTGCACGAACGCGTGATCGTGATCGACGTGTCCGAAGAC
AAGAACGAGCTGCGCGCCTTCATCAACCCCGAGATCATCTGGTCGAGCGACGGCAAGCAGGTCTACGAAGAGGGCTGTCT
GTCGGTGCCCGGCATCTACGACGAAGTCGAGCGCCCGGACCGCGTGCGCGTGCGCGCGCTCAACGAGCAGGGCGAGACGT
TCGAGCTCGACTGCGAAGGCCTGCTCGCCGTATGCATCCAGCACGAAATGGATCACCTGATGGGCCGCGTGTTCGTCGAA
TACCTGTCGCCGCTCAAACAGAGCCGGATCAAGACGAAGATGAAGAAACTCGAACGCGCGATGTGA

Upstream 100 bases:

>100_bases
CAGGCGCGCGGCCGAATGCAGGAGGCGCGATCCGCGGCATTGATTCGCCGCGCATCCGCCTCATCTTCATTGCATCGCGG
CGGCAGCGCCAGCCGGCCGG

Downstream 100 bases:

>100_bases
CGCGCGTTCGCCGAGACCGACCCCGAACGCTGTCATGACCCATACGTTGCGCGTGATTTTTGCCGGCACGCCGGAATTCG
CCGCGGCCGCGCTGGCCGCG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MANAAHRFTEYRKTMALLNILHYPDKRLHKVAKPVDKVDDRIRKLVADMAETMYAAPGIGLAATQVDVHERVIVIDVSED
KNELRAFINPEIIWSSDGKQVYEEGCLSVPGIYDEVERPDRVRVRALNEQGETFELDCEGLLAVCIQHEMDHLMGRVFVE
YLSPLKQSRIKTKMKKLERAM

Sequences:

>Translated_181_residues
MANAAHRFTEYRKTMALLNILHYPDKRLHKVAKPVDKVDDRIRKLVADMAETMYAAPGIGLAATQVDVHERVIVIDVSED
KNELRAFINPEIIWSSDGKQVYEEGCLSVPGIYDEVERPDRVRVRALNEQGETFELDCEGLLAVCIQHEMDHLMGRVFVE
YLSPLKQSRIKTKMKKLERAM
>Mature_180_residues
ANAAHRFTEYRKTMALLNILHYPDKRLHKVAKPVDKVDDRIRKLVADMAETMYAAPGIGLAATQVDVHERVIVIDVSEDK
NELRAFINPEIIWSSDGKQVYEEGCLSVPGIYDEVERPDRVRVRALNEQGETFELDCEGLLAVCIQHEMDHLMGRVFVEY
LSPLKQSRIKTKMKKLERAM

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=155, Percent_Identity=28.3870967741935, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI1789682, Length=165, Percent_Identity=57.5757575757576, Blast_Score=200, Evalue=5e-53,
Organism=Drosophila melanogaster, GI24645728, Length=146, Percent_Identity=33.5616438356164, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24645726, Length=137, Percent_Identity=29.9270072992701, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20781; Mature: 20650

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANAAHRFTEYRKTMALLNILHYPDKRLHKVAKPVDKVDDRIRKLVADMAETMYAAPGIG
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LAATQVDVHERVIVIDVSEDKNELRAFINPEIIWSSDGKQVYEEGCLSVPGIYDEVERPD
HHHHEEHHHCEEEEEECCCCHHHHHHHCCCCEEECCCCHHHHHHCCCCCCCCHHHHCCCC
RVRVRALNEQGETFELDCEGLLAVCIQHEMDHLMGRVFVEYLSPLKQSRIKTKMKKLERA
HHHHHEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
M
C
>Mature Secondary Structure 
ANAAHRFTEYRKTMALLNILHYPDKRLHKVAKPVDKVDDRIRKLVADMAETMYAAPGIG
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LAATQVDVHERVIVIDVSEDKNELRAFINPEIIWSSDGKQVYEEGCLSVPGIYDEVERPD
HHHHEEHHHCEEEEEECCCCHHHHHHHCCCCEEECCCCHHHHHHCCCCCCCCHHHHCCCC
RVRVRALNEQGETFELDCEGLLAVCIQHEMDHLMGRVFVEYLSPLKQSRIKTKMKKLERA
HHHHHEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
M
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]