Click here to switch to the map view.

The map label for this gene is cca [H]

Identifier: 161526195

GI number: 161526195

Start: 3307885

End: 3309126

Strand: Reverse

Name: cca [H]

Synonym: Bmul_3026

Alternate gene names: 161526195

Gene position: 3309126-3307885 (Counterclockwise)

Preceding gene: 161526196

Following gene: 161526193

Centisome position: 95.96

GC content: 70.29

Gene sequence:

>1242_bases
ATGAACATCTATGCCGTGGGCGGTGCGATCCGCGACGAACTGCTCGGCGTGCCGGTGCAGGACCGCGACTACGTGGTGGT
CGGCGCGACGCCCGAGCAGATGATCGCGCAGGGCTTCCGGCCGGTCGGCAAGGACTTCCCGGTGTTCCTGCATCCGGACA
CGCACGAGGAATACGCGCTTGCGCGCACCGAGCGCAAGACGGCGGCCGGCTATCACGGCTTCCAGTTCCACTACGCACCC
GACGTGACGCTCGACGAGGATCTCGCGCGCCGCGATCTGACGATCAATGCGATGGCGCGCGAGGTGAGCCCGGACGGCAC
GCTGATCGGCCCGGTCATCGATCCGTTCGACGGGCAGCGCGATTTGCGCGCGCGCGTGTTCCGGCACGTGAGCGACGCGT
TCGTCGAGGACCCGGTGCGGATTCTGCGGCTCGCGCGCTTTGCCGCGCGTTTTGCCGACTTCACGGTGGCCGACGATACG
CTCGCGCTGATGCGCCGGATGGTCGACGCGGGCGAAGTCGACGCGCTCGTGCCCGAGCGCGTATGGCAGGAGCTCGCGCG
CGGGCTCATGGAAGCGAAGCCGTCGCGGATGTTCGCGGTGCTGCGCGACTGCGGCGCGCTCGCGCGGATCCTGCCGGAGG
TCGACGGGCTGTGGGGCGTGCCGCAGCGCGCCGACTATCACCCGGAAGTCGACACGGGCGTGCACGTAATGATGGTCGTC
GACTACGCGGCGAAGCAGCGCTATTCGCTGCCGGTGCGCTTCGCGGCGCTCACGCACGATCTCGGCAAGGCCACGACGCC
CGACGACGTGCTGCCGCGCCACACCGGTCACGAAGGCCGCAGCGTCGATCTGCTGAAACCGCTGTGCGAGCGGCTGCGCG
TGCCGAACGAATGCCGCGACCTCGCGCTCGTCGTCGCGCGCGAGCACGGCAACCTGCATCGCGTGATGGAGATGGGCGCG
GCCGCGCTCGTGCGGTTCTTCGAACGCACCGACGCGCTGCGCAAGCCGGCGCGCTTCGCGGAGATGCTGCAGGCATGCGA
GGCGGATGCGCGCGGCCGGCTCGGGCTCGAAGCGCAGCCGTATCCGCAGGCCGAGCGCTTGCGTGTCGCACTCGCGGCCG
CGCGCAGCGTCGACGCGGGGGCGATTGCGCGCGCGACCGGCAGCGATTCGGCGAAGATCAAGGACGCCGTGCATCGCGCA
CGGATCGAGGCGGTTGCGCACGCGCTCGAGATCGGCGAGTAA

Upstream 100 bases:

>100_bases
CCGGCTATGCGGCACGCATCACCGCGCTGCCGGCGGTCCGGCAGTGGATCGACGCCGCGCGGCGCGAGACGAACGTGATT
GCCGAATACGAGCCGACGCG

Downstream 100 bases:

>100_bases
CGGCGGCGGACGGCGGCGGACGACGGGACGGCGTATGCGGTGCCGGCGACGGTGTGCGCCCGAACGCAGGCAGGGTCGGC
GGTAGGCGTAGGCGGCGGAC

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MNIYAVGGAIRDELLGVPVQDRDYVVVGATPEQMIAQGFRPVGKDFPVFLHPDTHEEYALARTERKTAAGYHGFQFHYAP
DVTLDEDLARRDLTINAMAREVSPDGTLIGPVIDPFDGQRDLRARVFRHVSDAFVEDPVRILRLARFAARFADFTVADDT
LALMRRMVDAGEVDALVPERVWQELARGLMEAKPSRMFAVLRDCGALARILPEVDGLWGVPQRADYHPEVDTGVHVMMVV
DYAAKQRYSLPVRFAALTHDLGKATTPDDVLPRHTGHEGRSVDLLKPLCERLRVPNECRDLALVVAREHGNLHRVMEMGA
AALVRFFERTDALRKPARFAEMLQACEADARGRLGLEAQPYPQAERLRVALAAARSVDAGAIARATGSDSAKIKDAVHRA
RIEAVAHALEIGE

Sequences:

>Translated_413_residues
MNIYAVGGAIRDELLGVPVQDRDYVVVGATPEQMIAQGFRPVGKDFPVFLHPDTHEEYALARTERKTAAGYHGFQFHYAP
DVTLDEDLARRDLTINAMAREVSPDGTLIGPVIDPFDGQRDLRARVFRHVSDAFVEDPVRILRLARFAARFADFTVADDT
LALMRRMVDAGEVDALVPERVWQELARGLMEAKPSRMFAVLRDCGALARILPEVDGLWGVPQRADYHPEVDTGVHVMMVV
DYAAKQRYSLPVRFAALTHDLGKATTPDDVLPRHTGHEGRSVDLLKPLCERLRVPNECRDLALVVAREHGNLHRVMEMGA
AALVRFFERTDALRKPARFAEMLQACEADARGRLGLEAQPYPQAERLRVALAAARSVDAGAIARATGSDSAKIKDAVHRA
RIEAVAHALEIGE
>Mature_413_residues
MNIYAVGGAIRDELLGVPVQDRDYVVVGATPEQMIAQGFRPVGKDFPVFLHPDTHEEYALARTERKTAAGYHGFQFHYAP
DVTLDEDLARRDLTINAMAREVSPDGTLIGPVIDPFDGQRDLRARVFRHVSDAFVEDPVRILRLARFAARFADFTVADDT
LALMRRMVDAGEVDALVPERVWQELARGLMEAKPSRMFAVLRDCGALARILPEVDGLWGVPQRADYHPEVDTGVHVMMVV
DYAAKQRYSLPVRFAALTHDLGKATTPDDVLPRHTGHEGRSVDLLKPLCERLRVPNECRDLALVVAREHGNLHRVMEMGA
AALVRFFERTDALRKPARFAEMLQACEADARGRLGLEAQPYPQAERLRVALAAARSVDAGAIARATGSDSAKIKDAVHRA
RIEAVAHALEIGE

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=412, Percent_Identity=52.6699029126214, Blast_Score=424, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 45773; Mature: 45773

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIYAVGGAIRDELLGVPVQDRDYVVVGATPEQMIAQGFRPVGKDFPVFLHPDTHEEYAL
CEEEEECCHHHHHHHCCCCCCCCEEEECCCHHHHHHHCCCCCCCCCCEEECCCCCHHHHH
ARTERKTAAGYHGFQFHYAPDVTLDEDLARRDLTINAMAREVSPDGTLIGPVIDPFDGQR
HHHHHHHHCCCCCEEEEECCCCCCCHHHHHHCCCHHHHHHHCCCCCCEECCCCCCCCCHH
DLRARVFRHVSDAFVEDPVRILRLARFAARFADFTVADDTLALMRRMVDAGEVDALVPER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHH
VWQELARGLMEAKPSRMFAVLRDCGALARILPEVDGLWGVPQRADYHPEVDTGVHVMMVV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEE
DYAAKQRYSLPVRFAALTHDLGKATTPDDVLPRHTGHEGRSVDLLKPLCERLRVPNECRD
EHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHH
LALVVAREHGNLHRVMEMGAAALVRFFERTDALRKPARFAEMLQACEADARGRLGLEAQP
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCC
YPQAERLRVALAAARSVDAGAIARATGSDSAKIKDAVHRARIEAVAHALEIGE
CCHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNIYAVGGAIRDELLGVPVQDRDYVVVGATPEQMIAQGFRPVGKDFPVFLHPDTHEEYAL
CEEEEECCHHHHHHHCCCCCCCCEEEECCCHHHHHHHCCCCCCCCCCEEECCCCCHHHHH
ARTERKTAAGYHGFQFHYAPDVTLDEDLARRDLTINAMAREVSPDGTLIGPVIDPFDGQR
HHHHHHHHCCCCCEEEEECCCCCCCHHHHHHCCCHHHHHHHCCCCCCEECCCCCCCCCHH
DLRARVFRHVSDAFVEDPVRILRLARFAARFADFTVADDTLALMRRMVDAGEVDALVPER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHH
VWQELARGLMEAKPSRMFAVLRDCGALARILPEVDGLWGVPQRADYHPEVDTGVHVMMVV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEE
DYAAKQRYSLPVRFAALTHDLGKATTPDDVLPRHTGHEGRSVDLLKPLCERLRVPNECRD
EHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHH
LALVVAREHGNLHRVMEMGAAALVRFFERTDALRKPARFAEMLQACEADARGRLGLEAQP
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCC
YPQAERLRVALAAARSVDAGAIARATGSDSAKIKDAVHRARIEAVAHALEIGE
CCHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA