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The map label for this gene is potF [H]

Identifier: 161526159

GI number: 161526159

Start: 3270322

End: 3271437

Strand: Direct

Name: potF [H]

Synonym: Bmul_2990

Alternate gene names: 161526159

Gene position: 3270322-3271437 (Clockwise)

Preceding gene: 161526158

Following gene: 161526160

Centisome position: 94.83

GC content: 62.81

Gene sequence:

>1116_bases
ATGCGTGCTCGCTTTCTTCGTCAAGCCTGCTCCGCCGCTGCCCTTGCCGCAGCAGCCGCGTTCACGTCCGTCGCCAGCCC
ATCGGCGCATGCCGACGAGCTGAACGTCTACAACTGGTCCGACTACATCGCGCCGGACACGATTCCGAACTTCCAGAAGC
AGACCGGCATCCACGTGAAGTACGACAACTACGACAGCGACGATACGCTGCAGGCCAAGCTGCTCGCCGGCAGCTCGGGC
TACGACATCGTCGTGCCGACGTCGAACTACATGGCCAAGCAGATTCAGGCCGGCGTGTACCAGAAGCTCGACAAATCGAA
GCTGCCGAATCTCGCGAACCTCGATCCGGTGCTGATGAAGATGATCTCGGACGCCGATCCGGGCAACCAGTACGGCGTGC
CGTGGGCATACGGCACCGACGGGATCGGCTACAACGTGCAGGCGGTGAAGAAGGCGCTCGGCGACAAGGCACCCGTCGAC
AGCTGGGCGCTCGTGTTCGATCCGGCCAACATGGAGAAGCTGAAGAGCTGCGGCGTGTCGATCCTCGATCAAGCCGTCGA
CGTGTTCGCGGCCACGCTGCAATACATGGGCAGGAATCCGAACAGCACGAATCCGGCCGACTATCAGGCCGCCTTCGACG
TGCTGAAGAAAGTCCGTCCGTACATCACGCAGTTCAACTCGTCGGGCTACATCAACGACCTCGCGAACAACGACGTCTGC
GTCGCGCTCGGCTGGTCGGGCGACGTCGGCATCGCGCATCGTCGCGCGGCCGAAGCGAAGCGCTCGTACGACATCCGCTT
TGCCAATCCGAAGGAAGGCGGGCTGCTGTGGTTCGACGTGATGGTGATCCCGAAGGACGCGCCGCATCCCGAGAACGCGC
TGAAGTGGATCAACTACGTGTCCGATCCGAAGGTCAACGCGGCCATCACGAACACCGTGTTCTATCCGACCGCGAACAAG
GCCGCGCGCCAGTTCGTGACGCCGGCCGTCGCGCAGGACCCGACCGTGTATCCGTCCGAGGAGGTGCTGAAGAAGATGGT
GCTGATGAAGCCGATGCCGGCCGACATCCTGCGGCTGGAAAACCGTCTGTGGGCGCAGCTGAAGACCGGCCACTGA

Upstream 100 bases:

>100_bases
GCCGTCGATGCGACCGCCCAGCAGCTGGGCCTTTCGTAACGGCTTTGCCTACAGGTGTGCGGCGGGCGCCGCACGCCGCC
ACAACCAGGGGAACTCCACC

Downstream 100 bases:

>100_bases
CCGCGATGCCGCACGGCGTCGCGCAGGCCGTCCGCCTGCCGCGCGCCTGACATTCGCGGACAGCGTCCGCCCGCGCGCGC
CGCGCGCACCTGTTCCATCC

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MRARFLRQACSAAALAAAAAFTSVASPSAHADELNVYNWSDYIAPDTIPNFQKQTGIHVKYDNYDSDDTLQAKLLAGSSG
YDIVVPTSNYMAKQIQAGVYQKLDKSKLPNLANLDPVLMKMISDADPGNQYGVPWAYGTDGIGYNVQAVKKALGDKAPVD
SWALVFDPANMEKLKSCGVSILDQAVDVFAATLQYMGRNPNSTNPADYQAAFDVLKKVRPYITQFNSSGYINDLANNDVC
VALGWSGDVGIAHRRAAEAKRSYDIRFANPKEGGLLWFDVMVIPKDAPHPENALKWINYVSDPKVNAAITNTVFYPTANK
AARQFVTPAVAQDPTVYPSEEVLKKMVLMKPMPADILRLENRLWAQLKTGH

Sequences:

>Translated_371_residues
MRARFLRQACSAAALAAAAAFTSVASPSAHADELNVYNWSDYIAPDTIPNFQKQTGIHVKYDNYDSDDTLQAKLLAGSSG
YDIVVPTSNYMAKQIQAGVYQKLDKSKLPNLANLDPVLMKMISDADPGNQYGVPWAYGTDGIGYNVQAVKKALGDKAPVD
SWALVFDPANMEKLKSCGVSILDQAVDVFAATLQYMGRNPNSTNPADYQAAFDVLKKVRPYITQFNSSGYINDLANNDVC
VALGWSGDVGIAHRRAAEAKRSYDIRFANPKEGGLLWFDVMVIPKDAPHPENALKWINYVSDPKVNAAITNTVFYPTANK
AARQFVTPAVAQDPTVYPSEEVLKKMVLMKPMPADILRLENRLWAQLKTGH
>Mature_371_residues
MRARFLRQACSAAALAAAAAFTSVASPSAHADELNVYNWSDYIAPDTIPNFQKQTGIHVKYDNYDSDDTLQAKLLAGSSG
YDIVVPTSNYMAKQIQAGVYQKLDKSKLPNLANLDPVLMKMISDADPGNQYGVPWAYGTDGIGYNVQAVKKALGDKAPVD
SWALVFDPANMEKLKSCGVSILDQAVDVFAATLQYMGRNPNSTNPADYQAAFDVLKKVRPYITQFNSSGYINDLANNDVC
VALGWSGDVGIAHRRAAEAKRSYDIRFANPKEGGLLWFDVMVIPKDAPHPENALKWINYVSDPKVNAAITNTVFYPTANK
AARQFVTPAVAQDPTVYPSEEVLKKMVLMKPMPADILRLENRLWAQLKTGH

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=366, Percent_Identity=48.3606557377049, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1787367, Length=321, Percent_Identity=37.6947040498442, Blast_Score=207, Evalue=7e-55,
Organism=Escherichia coli, GI1787711, Length=331, Percent_Identity=24.773413897281, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40690; Mature: 40690

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRARFLRQACSAAALAAAAAFTSVASPSAHADELNVYNWSDYIAPDTIPNFQKQTGIHVK
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCHHHHCCCEEE
YDNYDSDDTLQAKLLAGSSGYDIVVPTSNYMAKQIQAGVYQKLDKSKLPNLANLDPVLMK
ECCCCCCCCEEEEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
MISDADPGNQYGVPWAYGTDGIGYNVQAVKKALGDKAPVDSWALVFDPANMEKLKSCGVS
HHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHCCHH
ILDQAVDVFAATLQYMGRNPNSTNPADYQAAFDVLKKVRPYITQFNSSGYINDLANNDVC
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCEE
VALGWSGDVGIAHRRAAEAKRSYDIRFANPKEGGLLWFDVMVIPKDAPHPENALKWINYV
EEECCCCCCCHHHHHHHHHHHCCCEEECCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHC
SDPKVNAAITNTVFYPTANKAARQFVTPAVAQDPTVYPSEEVLKKMVLMKPMPADILRLE
CCCCCCEEEECEEEECCCCHHHHHHHCCHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHH
NRLWAQLKTGH
HHHHHHCCCCC
>Mature Secondary Structure
MRARFLRQACSAAALAAAAAFTSVASPSAHADELNVYNWSDYIAPDTIPNFQKQTGIHVK
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCHHHHCCCEEE
YDNYDSDDTLQAKLLAGSSGYDIVVPTSNYMAKQIQAGVYQKLDKSKLPNLANLDPVLMK
ECCCCCCCCEEEEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
MISDADPGNQYGVPWAYGTDGIGYNVQAVKKALGDKAPVDSWALVFDPANMEKLKSCGVS
HHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHCCHH
ILDQAVDVFAATLQYMGRNPNSTNPADYQAAFDVLKKVRPYITQFNSSGYINDLANNDVC
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCEE
VALGWSGDVGIAHRRAAEAKRSYDIRFANPKEGGLLWFDVMVIPKDAPHPENALKWINYV
EEECCCCCCCHHHHHHHHHHHCCCEEECCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHC
SDPKVNAAITNTVFYPTANKAARQFVTPAVAQDPTVYPSEEVLKKMVLMKPMPADILRLE
CCCCCCEEEECEEEECCCCHHHHHHHCCHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHH
NRLWAQLKTGH
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]