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The map label for this gene is puuA [H]

Identifier: 161526157

GI number: 161526157

Start: 3267410

End: 3268804

Strand: Direct

Name: puuA [H]

Synonym: Bmul_2988

Alternate gene names: 161526157

Gene position: 3267410-3268804 (Clockwise)

Preceding gene: 161526156

Following gene: 161526158

Centisome position: 94.75

GC content: 64.23

Gene sequence:

>1395_bases
ATGCCCGGCCCGCCGCTGCGCTCGCGTGAGCGCGCCGACCCACTTACGAGAACGAACATGCAAGACATCGAAGACTTTCT
GAAGCAGCACCGGATTACCGAGATCGAAGCGATCATTCCCGACATGGCCGGCATCGCGCGCGGCAAGATCACGCCGCGCA
ACAAGTTCACGTCCGGCGAATCGATGCGGCTGCCGCAGGCCGTGATGGTGCAGACCGTCACCGGCGACTATCCGGAAGAC
GGGTCGCTCTCCGGCGTCACCGATCCCGACATGGTCTGCGTACCCGACACGTCGACGATCCGGCTGATCCCGTGGGCCGT
CGATCCGACCGCGCAGGTGATCCACGACTGCGTGCACTTCGACGGCTCGCCGGTCGAGATCTCGCCGCGCTACGTGCTGC
GCCGCGTGCTCGACCTGTACAAGAAGAAAGGCTGGAAGCCGGTCGTCGCGCCCGAACTCGAGTTCTATCTGGTCGACATG
AACAAGGATCCCGACCTGCCGCTGCGTCCGCCGGTCGGCCGCACGGGTCGGCCGGAAACGGGCCGCCAGTCGTATTCGAT
CGAGGCCGTCAACGAGTTCGATCCGCTGTTCGAAGACATCTACGAATACTGCGAGACGCAGGACCTCGACATCGACACGC
TGATCCACGAAGTCGGCGCCGCGCAGATGGAGATCAACTTCATGCACGGCGATGCGCTGTCGCTCGCCGATCAGGTGTTC
CTGTTCAAGCGCACGGTGCGCGAAGCGGCGTTGCGTCACAACATGTACGCGACCTTCATGGCGAAGCCGATGGAAGGCGA
GCCGGGCTCGGCGATGCATGTGCACCAGAGCATCGTCGACGAGGAAAGCGGCCAGAACCTGTTCACGTCGCCGGAAACGG
GCGGCGCGACCTCGATGTTCTACAACTTCCTCGCCGGGCTGCAGAAGTACACGCCCGCGCTGATGCCGATCTTCGCGCCG
TACATCAACTCGTATCGCCGGCTGTCGCGCTTCATGGCCGCGCCGATCAACGTGCAGTGGGGCTACGACAACCGCACGGT
CGGCTTTCGCATTCCGCATTCGAGCCCGGCCGCGCGGCGCATCGAGAACCGGATTCCGGGCGTCGACTGCAATCCGTATC
TCGCGCTCGCCGCGACGCTCGCGGCCGGCTATCTCGGCATCACGCAGCGGCTCGAGCCGACCGAGCCGCTCGTCAGCGAC
GGCTACTCGCTGCCGTATCAGCTGCCGCGCAATCTCGAGGACGGGCTCACGCTGATGTCGGCATGCGAGCCGCTCGGCGA
GATCCTCGGCCACAAGTTCCTGAAAGCGTATTTCGCGCTGAAGGAGACCGAATACGAAGCGTTCTTCCGCGTGATCAGCT
CGTGGGAACGCCGTCATCTGCTGCTGCACGTGTAA

Upstream 100 bases:

>100_bases
CAATGGCATCCGGAATGGCGGCACGCGCACGACGCGCTGTCGAGCGCGATCTTCCGCGCGTTCGGCGACGCGTGCCGCGC
GCGCCGCGACGCGCGTTCGC

Downstream 100 bases:

>100_bases
GCGCGCACAACCGCAATCCGGAGGAAACATGACCTATCGCAACGAATCCGCGTGGATCCAGCCCGCCGCACCGGCGGCCC
GCTCGACGCAGGCGCGCTCG

Product: glutamate--putrescine ligase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MPGPPLRSRERADPLTRTNMQDIEDFLKQHRITEIEAIIPDMAGIARGKITPRNKFTSGESMRLPQAVMVQTVTGDYPED
GSLSGVTDPDMVCVPDTSTIRLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDLYKKKGWKPVVAPELEFYLVDM
NKDPDLPLRPPVGRTGRPETGRQSYSIEAVNEFDPLFEDIYEYCETQDLDIDTLIHEVGAAQMEINFMHGDALSLADQVF
LFKRTVREAALRHNMYATFMAKPMEGEPGSAMHVHQSIVDEESGQNLFTSPETGGATSMFYNFLAGLQKYTPALMPIFAP
YINSYRRLSRFMAAPINVQWGYDNRTVGFRIPHSSPAARRIENRIPGVDCNPYLALAATLAAGYLGITQRLEPTEPLVSD
GYSLPYQLPRNLEDGLTLMSACEPLGEILGHKFLKAYFALKETEYEAFFRVISSWERRHLLLHV

Sequences:

>Translated_464_residues
MPGPPLRSRERADPLTRTNMQDIEDFLKQHRITEIEAIIPDMAGIARGKITPRNKFTSGESMRLPQAVMVQTVTGDYPED
GSLSGVTDPDMVCVPDTSTIRLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDLYKKKGWKPVVAPELEFYLVDM
NKDPDLPLRPPVGRTGRPETGRQSYSIEAVNEFDPLFEDIYEYCETQDLDIDTLIHEVGAAQMEINFMHGDALSLADQVF
LFKRTVREAALRHNMYATFMAKPMEGEPGSAMHVHQSIVDEESGQNLFTSPETGGATSMFYNFLAGLQKYTPALMPIFAP
YINSYRRLSRFMAAPINVQWGYDNRTVGFRIPHSSPAARRIENRIPGVDCNPYLALAATLAAGYLGITQRLEPTEPLVSD
GYSLPYQLPRNLEDGLTLMSACEPLGEILGHKFLKAYFALKETEYEAFFRVISSWERRHLLLHV
>Mature_463_residues
PGPPLRSRERADPLTRTNMQDIEDFLKQHRITEIEAIIPDMAGIARGKITPRNKFTSGESMRLPQAVMVQTVTGDYPEDG
SLSGVTDPDMVCVPDTSTIRLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDLYKKKGWKPVVAPELEFYLVDMN
KDPDLPLRPPVGRTGRPETGRQSYSIEAVNEFDPLFEDIYEYCETQDLDIDTLIHEVGAAQMEINFMHGDALSLADQVFL
FKRTVREAALRHNMYATFMAKPMEGEPGSAMHVHQSIVDEESGQNLFTSPETGGATSMFYNFLAGLQKYTPALMPIFAPY
INSYRRLSRFMAAPINVQWGYDNRTVGFRIPHSSPAARRIENRIPGVDCNPYLALAATLAAGYLGITQRLEPTEPLVSDG
YSLPYQLPRNLEDGLTLMSACEPLGEILGHKFLKAYFALKETEYEAFFRVISSWERRHLLLHV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=453, Percent_Identity=26.7108167770419, Blast_Score=149, Evalue=5e-36,
Organism=Escherichia coli, GI87081870, Length=451, Percent_Identity=34.1463414634146, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1790301, Length=399, Percent_Identity=29.0726817042606, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 52317; Mature: 52186

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGPPLRSRERADPLTRTNMQDIEDFLKQHRITEIEAIIPDMAGIARGKITPRNKFTSGE
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
SMRLPQAVMVQTVTGDYPEDGSLSGVTDPDMVCVPDTSTIRLIPWAVDPTAQVIHDCVHF
CCCCCHHHHEEECCCCCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCHHHHHHHHHHHC
DGSPVEISPRYVLRRVLDLYKKKGWKPVVAPELEFYLVDMNKDPDLPLRPPVGRTGRPET
CCCCEEECHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCC
GRQSYSIEAVNEFDPLFEDIYEYCETQDLDIDTLIHEVGAAQMEINFMHGDALSLADQVF
CCCCEEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHH
LFKRTVREAALRHNMYATFMAKPMEGEPGSAMHVHQSIVDEESGQNLFTSPETGGATSMF
HHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
YNFLAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQWGYDNRTVGFRIPHSSPAARR
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEECCCCCHHHHH
IENRIPGVDCNPYLALAATLAAGYLGITQRLEPTEPLVSDGYSLPYQLPRNLEDGLTLMS
HHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHH
ACEPLGEILGHKFLKAYFALKETEYEAFFRVISSWERRHLLLHV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEC
>Mature Secondary Structure 
PGPPLRSRERADPLTRTNMQDIEDFLKQHRITEIEAIIPDMAGIARGKITPRNKFTSGE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
SMRLPQAVMVQTVTGDYPEDGSLSGVTDPDMVCVPDTSTIRLIPWAVDPTAQVIHDCVHF
CCCCCHHHHEEECCCCCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCHHHHHHHHHHHC
DGSPVEISPRYVLRRVLDLYKKKGWKPVVAPELEFYLVDMNKDPDLPLRPPVGRTGRPET
CCCCEEECHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCC
GRQSYSIEAVNEFDPLFEDIYEYCETQDLDIDTLIHEVGAAQMEINFMHGDALSLADQVF
CCCCEEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHH
LFKRTVREAALRHNMYATFMAKPMEGEPGSAMHVHQSIVDEESGQNLFTSPETGGATSMF
HHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
YNFLAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQWGYDNRTVGFRIPHSSPAARR
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEECCCCCHHHHH
IENRIPGVDCNPYLALAATLAAGYLGITQRLEPTEPLVSDGYSLPYQLPRNLEDGLTLMS
HHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHH
ACEPLGEILGHKFLKAYFALKETEYEAFFRVISSWERRHLLLHV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]