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The map label for this gene is purR [H]

Identifier: 161526136

GI number: 161526136

Start: 3249589

End: 3250608

Strand: Direct

Name: purR [H]

Synonym: Bmul_2967

Alternate gene names: 161526136

Gene position: 3249589-3250608 (Clockwise)

Preceding gene: 161526135

Following gene: 161526137

Centisome position: 94.23

GC content: 71.47

Gene sequence:

>1020_bases
ATGGGCACCACCATTCGCGACGTGGCGCGGGCGGCAGAGGTCTCGATCGGCACCGTGTCGCGCGCGCTGAAAAATCAGCC
GGGCCTGTCCGAAACGACGCGCGCGCGGATCGTCGAGATTGCGCAGCGGCTCGGCTACGACCACGCGCAGCTGCGGCCGC
GCATCCGCCGGCTCACGTTCCTGCTGCATCGGCAGCACAACCGCTTTCCGGCGAGCCCGTTCTTCTCGCACGTGCTGCAC
GGCGTCGAGGACGCCTGCCGCGAACGCGGCATCGTGCCGACGCTGCTGACGGTCGGCCCGAACGACGACGTGCTGCGCCA
GATGCGTCCGCACGCGCCCGACGCGATCGCGGTGGCCGGCTTCATCGAACCGGAAACGATCGAGGCGCTCGCCGCGACCG
GCCGCCCGCTCGTGCTGATCGACCTGTGGGCGCCTGGCCTGCGCTCGGTCAACATCGACAACGCGACCGGCGCCGCACTC
GCGATGCGCCATCTGCTGGCAACGGGCCGTTCGCGGATCGCCTTCATCGGCGGCTCGCCCGCGCACTACAGCATCGCGCA
GCGCGCGATCGGCTATCGCCGCGCATTCTTCGAGGCCGGCCGGCTGTTCGATCCCGCGTACGAAGTGACGATCGACGCAG
GGCTCGATCCCGACACCGGCGCCACGCGCGCGATGCAGCAGCTGCTCGACGCGCCGGGCCCGCGGCCCGACGCGGTGTTC
GCGTACAACGACGCGGCCGCGCTGGCCGCGCAGCGCGTATGCCTCGCTCGCGGCCTGCGGATTCCGGACGACATCGCGAT
CGTCGGCTTCGACGACATTCCCGCCGCCGCGCACGCGAACCCGCCGCTGACGACGCTCGCGGTCGACAAGGAAGCGCTCG
GCCGCCGCGGCGTCGAACTGCTGCTCGCCGAGTCGCCCGAACACACCGAGATCTCGCTGCCCGTCGAGCTGATCGTGCGG
GCCAGCAGCCAGTCCGCCGGCTCGCCGGCCCTCGATACCGCCACGGTCACCGAATCATGA

Upstream 100 bases:

>100_bases
AAGCGGCTATTTGCGGGTATTCCCCGGTTATCCGACCCGTTTTCCCGAACAAGTTTTACTATACAATCGCCGTCAACCTA
AACATTCCGGAACCCGAACG

Downstream 100 bases:

>100_bases
ACATGCCTCCGGTCCAGTCCTGCCCCGCCACGCCGGCCGCCCATGCGCAGCCGGCGCCGTTCGTCGCGAACTTCCGCGAT
CCGTCGTTCCTGCTGTCGCA

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MGTTIRDVARAAEVSIGTVSRALKNQPGLSETTRARIVEIAQRLGYDHAQLRPRIRRLTFLLHRQHNRFPASPFFSHVLH
GVEDACRERGIVPTLLTVGPNDDVLRQMRPHAPDAIAVAGFIEPETIEALAATGRPLVLIDLWAPGLRSVNIDNATGAAL
AMRHLLATGRSRIAFIGGSPAHYSIAQRAIGYRRAFFEAGRLFDPAYEVTIDAGLDPDTGATRAMQQLLDAPGPRPDAVF
AYNDAAALAAQRVCLARGLRIPDDIAIVGFDDIPAAAHANPPLTTLAVDKEALGRRGVELLLAESPEHTEISLPVELIVR
ASSQSAGSPALDTATVTES

Sequences:

>Translated_339_residues
MGTTIRDVARAAEVSIGTVSRALKNQPGLSETTRARIVEIAQRLGYDHAQLRPRIRRLTFLLHRQHNRFPASPFFSHVLH
GVEDACRERGIVPTLLTVGPNDDVLRQMRPHAPDAIAVAGFIEPETIEALAATGRPLVLIDLWAPGLRSVNIDNATGAAL
AMRHLLATGRSRIAFIGGSPAHYSIAQRAIGYRRAFFEAGRLFDPAYEVTIDAGLDPDTGATRAMQQLLDAPGPRPDAVF
AYNDAAALAAQRVCLARGLRIPDDIAIVGFDDIPAAAHANPPLTTLAVDKEALGRRGVELLLAESPEHTEISLPVELIVR
ASSQSAGSPALDTATVTES
>Mature_338_residues
GTTIRDVARAAEVSIGTVSRALKNQPGLSETTRARIVEIAQRLGYDHAQLRPRIRRLTFLLHRQHNRFPASPFFSHVLHG
VEDACRERGIVPTLLTVGPNDDVLRQMRPHAPDAIAVAGFIEPETIEALAATGRPLVLIDLWAPGLRSVNIDNATGAALA
MRHLLATGRSRIAFIGGSPAHYSIAQRAIGYRRAFFEAGRLFDPAYEVTIDAGLDPDTGATRAMQQLLDAPGPRPDAVFA
YNDAAALAAQRVCLARGLRIPDDIAIVGFDDIPAAAHANPPLTTLAVDKEALGRRGVELLLAESPEHTEISLPVELIVRA
SSQSAGSPALDTATVTES

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=352, Percent_Identity=31.5340909090909, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1790194, Length=313, Percent_Identity=32.2683706070287, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1789456, Length=320, Percent_Identity=30, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1787948, Length=328, Percent_Identity=28.6585365853659, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1789202, Length=294, Percent_Identity=29.9319727891156, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788474, Length=328, Percent_Identity=29.5731707317073, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI48994940, Length=314, Percent_Identity=28.343949044586, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787580, Length=315, Percent_Identity=26.031746031746, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1789068, Length=303, Percent_Identity=25.7425742574257, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786540, Length=352, Percent_Identity=26.9886363636364, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1787906, Length=354, Percent_Identity=25.7062146892655, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36360; Mature: 36229

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTTIRDVARAAEVSIGTVSRALKNQPGLSETTRARIVEIAQRLGYDHAQLRPRIRRLTF
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LLHRQHNRFPASPFFSHVLHGVEDACRERGIVPTLLTVGPNDDVLRQMRPHAPDAIAVAG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCCCCEEEEE
FIEPETIEALAATGRPLVLIDLWAPGLRSVNIDNATGAALAMRHLLATGRSRIAFIGGSP
CCCHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCHHHHHHHHHHHCCCCEEEEECCCC
AHYSIAQRAIGYRRAFFEAGRLFDPAYEVTIDAGLDPDTGATRAMQQLLDAPGPRPDAVF
CHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEE
AYNDAAALAAQRVCLARGLRIPDDIAIVGFDDIPAAAHANPPLTTLAVDKEALGRRGVEL
EECCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECHHHHCCCCEEE
LLAESPEHTEISLPVELIVRASSQSAGSPALDTATVTES
EEECCCCCCEEECCHHHEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GTTIRDVARAAEVSIGTVSRALKNQPGLSETTRARIVEIAQRLGYDHAQLRPRIRRLTF
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LLHRQHNRFPASPFFSHVLHGVEDACRERGIVPTLLTVGPNDDVLRQMRPHAPDAIAVAG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCCCCEEEEE
FIEPETIEALAATGRPLVLIDLWAPGLRSVNIDNATGAALAMRHLLATGRSRIAFIGGSP
CCCHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCHHHHHHHHHHHCCCCEEEEECCCC
AHYSIAQRAIGYRRAFFEAGRLFDPAYEVTIDAGLDPDTGATRAMQQLLDAPGPRPDAVF
CHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEE
AYNDAAALAAQRVCLARGLRIPDDIAIVGFDDIPAAAHANPPLTTLAVDKEALGRRGVEL
EECCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECHHHHCCCCEEE
LLAESPEHTEISLPVELIVRASSQSAGSPALDTATVTES
EEECCCCCCEEECCHHHEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA