BacMap

Definition	Burkholderia multivorans ATCC 17616 chromosome chromosome 1, complete sequence.
Accession	NC_010084
Length	3,448,466

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The map label for this gene is fadB1 [H]

Identifier: 161526019

GI number: 161526019

Start: 3114012

End: 3114788

Strand: Reverse

Name: fadB1 [H]

Synonym: Bmul_2850

Alternate gene names: 161526019

Gene position: 3114788-3114012 (Counterclockwise)

Preceding gene: 161526025

Following gene: 161526018

Centisome position: 90.32

GC content: 64.86

Gene sequence:

>777_bases
ATGTCTTACGAGAACATCCTGGTGGAGACGCGGGGGCGTGTCGGACTGATCACGCTGAACCGGCCGAAGGCGCTGAACGC
GTTGAACGACGCGTTGATGGATGAGCTGGGCGCGGCGCTGAAGGCGTTCGACGCGGACGACGCCGTGGGCGCGATCGTCG
TAACGGGAAGCGAGAAGGCCTTCGCGGCCGGAGCGGACATCGGCATGATGGCCACCTATTCCTATATGGACGTGTACCGC
GGCGACTACATCACGCGCAACTGGGAGACGGTGCGCCAGATCCGCAAGCCGATCATTGCAGCGGTCGCCGGTTTTGCGCT
CGGTGGCGGCTGCGAGCTGGCGATGATGTGCGACATCATCTTCGCGGCCGATACGGCGAAATTCGGCCAGCCGGAAATCA
AGCTGGGCGTGATGCCGGGCGCCGGCGGCACGCAGCGCCTGCCGCGCGCGATATCGAAGGCGAAGGCCATGGACATGTGC
CTGACTGCGCGCTTCATGGATGCGGACGAGGCGGAGCGCGCCGGGCTCGTATCGCGCGTGCTGCCGGCGGACAAGCTGCT
CGACGAGGCGTTGTCGGCTGCGGCGACGATCGCCGAGTTTTCGCTGCCCGCGGTCATGATGATCAAGGAGTCGGTGAATC
GGGCGTACGAGACGACGCTGGCGGAAGGTGTTCATTTCGAGCGCCGGCTGTTCCACTCGCTGTTCGCGACCGAGGATCAG
AAGGAAGGGATGGCGGCGTTCGTCGAGAAGCGCAAGCCGGTGTTCAAGAACCGGTGA

Upstream 100 bases:

>100_bases
TTGGATCGATTAATTAACCGACCGCTTGGTCGGAGAATGGTAGCATCAAACTATTCGCGTGTGCGAGGCGTTTCTCAATT
CATTGATCGAGGAGAAATAG

Downstream 100 bases:

>100_bases
TGGCATTGCGCGGCGGTTGCGGCGTTTGATGCCGACGGCGCGATGGGTAACACGCAGTCGAACCCGCGCGTCGCCCCCGA
AGCCCCCGTGCGCGGCAGCG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSYENILVETRGRVGLITLNRPKALNALNDALMDELGAALKAFDADDAVGAIVVTGSEKAFAAGADIGMMATYSYMDVYR
GDYITRNWETVRQIRKPIIAAVAGFALGGGCELAMMCDIIFAADTAKFGQPEIKLGVMPGAGGTQRLPRAISKAKAMDMC
LTARFMDADEAERAGLVSRVLPADKLLDEALSAAATIAEFSLPAVMMIKESVNRAYETTLAEGVHFERRLFHSLFATEDQ
KEGMAAFVEKRKPVFKNR

Sequences:

>Translated_258_residues
MSYENILVETRGRVGLITLNRPKALNALNDALMDELGAALKAFDADDAVGAIVVTGSEKAFAAGADIGMMATYSYMDVYR
GDYITRNWETVRQIRKPIIAAVAGFALGGGCELAMMCDIIFAADTAKFGQPEIKLGVMPGAGGTQRLPRAISKAKAMDMC
LTARFMDADEAERAGLVSRVLPADKLLDEALSAAATIAEFSLPAVMMIKESVNRAYETTLAEGVHFERRLFHSLFATEDQ
KEGMAAFVEKRKPVFKNR
>Mature_257_residues
SYENILVETRGRVGLITLNRPKALNALNDALMDELGAALKAFDADDAVGAIVVTGSEKAFAAGADIGMMATYSYMDVYRG
DYITRNWETVRQIRKPIIAAVAGFALGGGCELAMMCDIIFAADTAKFGQPEIKLGVMPGAGGTQRLPRAISKAKAMDMCL
TARFMDADEAERAGLVSRVLPADKLLDEALSAAATIAEFSLPAVMMIKESVNRAYETTLAEGVHFERRLFHSLFATEDQK
EGMAAFVEKRKPVFKNR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=260, Percent_Identity=56.5384615384615, Blast_Score=296, Evalue=1e-80,
Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=37.5, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI70995211, Length=267, Percent_Identity=34.4569288389513, Blast_Score=118, Evalue=5e-27,
Organism=Homo sapiens, GI31542718, Length=245, Percent_Identity=29.3877551020408, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI68989263, Length=192, Percent_Identity=36.4583333333333, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI20127408, Length=187, Percent_Identity=34.2245989304813, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI150378541, Length=244, Percent_Identity=32.3770491803279, Blast_Score=98, Evalue=9e-21,
Organism=Homo sapiens, GI37594471, Length=212, Percent_Identity=30.188679245283, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI37594469, Length=212, Percent_Identity=30.188679245283, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI45643119, Length=246, Percent_Identity=27.6422764227642, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI260275230, Length=246, Percent_Identity=27.6422764227642, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI260274832, Length=246, Percent_Identity=27.6422764227642, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI4757968, Length=257, Percent_Identity=24.9027237354086, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI49355787, Length=257, Percent_Identity=24.9027237354086, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI25453481, Length=256, Percent_Identity=24.609375, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI51538573, Length=256, Percent_Identity=24.609375, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI157694516, Length=228, Percent_Identity=25.8771929824561, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI213417737, Length=234, Percent_Identity=25.6410256410256, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI4757966, Length=211, Percent_Identity=27.0142180094787, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI51538581, Length=211, Percent_Identity=27.0142180094787, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI221307494, Length=185, Percent_Identity=29.7297297297297, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI221136756, Length=185, Percent_Identity=29.7297297297297, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI221136753, Length=185, Percent_Identity=29.7297297297297, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI261878539, Length=97, Percent_Identity=39.1752577319588, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI157694520, Length=206, Percent_Identity=25.7281553398058, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI116235487, Length=167, Percent_Identity=30.5389221556886, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=45.0592885375494, Blast_Score=217, Evalue=6e-58,
Organism=Escherichia coli, GI221142681, Length=260, Percent_Identity=36.1538461538462, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1788597, Length=260, Percent_Identity=33.4615384615385, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1787660, Length=269, Percent_Identity=31.9702602230483, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI87082183, Length=263, Percent_Identity=32.6996197718631, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1788682, Length=189, Percent_Identity=32.8042328042328, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1790281, Length=178, Percent_Identity=30.3370786516854, Blast_Score=92, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=251, Percent_Identity=56.1752988047809, Blast_Score=291, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI25145438, Length=243, Percent_Identity=53.9094650205761, Blast_Score=262, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI17560910, Length=254, Percent_Identity=34.6456692913386, Blast_Score=121, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17540306, Length=237, Percent_Identity=32.4894514767932, Blast_Score=117, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17540714, Length=220, Percent_Identity=30.9090909090909, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17558304, Length=182, Percent_Identity=36.2637362637363, Blast_Score=110, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI25144276, Length=182, Percent_Identity=35.1648351648352, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17508953, Length=183, Percent_Identity=36.0655737704918, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17508951, Length=182, Percent_Identity=35.1648351648352, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17536985, Length=273, Percent_Identity=28.9377289377289, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17534483, Length=256, Percent_Identity=26.171875, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI25144160, Length=217, Percent_Identity=31.3364055299539, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25144157, Length=217, Percent_Identity=31.3364055299539, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=179, Percent_Identity=33.5195530726257, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25141363, Length=199, Percent_Identity=28.1407035175879, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=259, Percent_Identity=24.7104247104247, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=193, Percent_Identity=27.4611398963731, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI20129971, Length=259, Percent_Identity=57.9150579150579, Blast_Score=301, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24653477, Length=259, Percent_Identity=57.9150579150579, Blast_Score=301, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24653139, Length=266, Percent_Identity=37.218045112782, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI21357171, Length=256, Percent_Identity=33.203125, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24650670, Length=213, Percent_Identity=33.3333333333333, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI19920382, Length=255, Percent_Identity=32.156862745098, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI19922422, Length=253, Percent_Identity=26.8774703557312, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24654903, Length=191, Percent_Identity=27.7486910994764, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI19921000, Length=170, Percent_Identity=36.4705882352941, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24583077, Length=170, Percent_Identity=36.4705882352941, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24583079, Length=170, Percent_Identity=36.4705882352941, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI28571729, Length=244, Percent_Identity=27.0491803278689, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28571730, Length=244, Percent_Identity=27.0491803278689, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27958; Mature: 27827

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYENILVETRGRVGLITLNRPKALNALNDALMDELGAALKAFDADDAVGAIVVTGSEKA
CCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
FAAGADIGMMATYSYMDVYRGDYITRNWETVRQIRKPIIAAVAGFALGGGCELAMMCDII
EECCCCCHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
FAADTAKFGQPEIKLGVMPGAGGTQRLPRAISKAKAMDMCLTARFMDADEAERAGLVSRV
HHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LPADKLLDEALSAAATIAEFSLPAVMMIKESVNRAYETTLAEGVHFERRLFHSLFATEDQ
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCH
KEGMAAFVEKRKPVFKNR
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SYENILVETRGRVGLITLNRPKALNALNDALMDELGAALKAFDADDAVGAIVVTGSEKA
CCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
FAAGADIGMMATYSYMDVYRGDYITRNWETVRQIRKPIIAAVAGFALGGGCELAMMCDII
EECCCCCHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
FAADTAKFGQPEIKLGVMPGAGGTQRLPRAISKAKAMDMCLTARFMDADEAERAGLVSRV
HHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LPADKLLDEALSAAATIAEFSLPAVMMIKESVNRAYETTLAEGVHFERRLFHSLFATEDQ
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCH
KEGMAAFVEKRKPVFKNR
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]