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The map label for this gene is yoaE [H]

Identifier: 161526018

GI number: 161526018

Start: 3105889

End: 3107964

Strand: Reverse

Name: yoaE [H]

Synonym: Bmul_2849

Alternate gene names: 161526018

Gene position: 3107964-3105889 (Counterclockwise)

Preceding gene: 161526019

Following gene: 161526017

Centisome position: 90.13

GC content: 66.04

Gene sequence:

>2076_bases
ATGAACGCCGCCACCCACGTTGCTCGGGCCGTCTGCCCGCATGATTGTCCGGATACGTGCGCCATGCGCGTGACTGTCGA
GAACGGAAGGGCGATCAAGGTCGCGGGCGACCCCGACCATCCGCCGACGCAAGGAGTCCTCTGCACGAAGGTCAGCCGAT
ATGCGGAGCGTGTTCATCATCCGGACCGTCTTACCGTGCCGCTGAAGCGCATCGGCGCGAAAGGCGAAGGGCGCTTCGCG
CCAATCAGCTGGGACGAGGCGTTCGACGAAATCGGCCGCCGTCTGGGGGACATTGCGACGCGCGCGCCCGAAGCGATCGT
GCCGTACAGCTATGCGGGAACGATGGGTCTCGTGCAGGGCGAAGGCATCGCGCAGCGCTTCTTCCACAAGATCGGCGCGT
CACGGCTCGAGCGCACGATCTGCGCGGCGGCCGGCGCCGCCGGCCTGCGCTTCACGTATGGCGCAAGCGTCGGGATGCAT
CTCGAGCATTTCGAGGAAAGCGAGCTGATCCTGATCTGGGGCGCCAATCCGATCGCATCGAGCCTGCATTTCTGGACCCG
CGCGCAGGAAGCCAAGCGGCGTGGCGCGCGGCTCGTCGCGATCGATCCGTATCGGTCGCTCACCGCCGAAAAGTGCCACC
AGCACATCGCGCTGAAGCCCGGCACCGACGGGGCATTCGCGCTCGGCATGATGCACGTGCTGATCACCGAGAATCTGCTC
GATCACGATTACATCGCCGACCATACGCTCGGATTCGACGCACTCAAGGCGCGCGCGCTGTCGTATCCGCCCGAGCGCGT
CGCGCAGATTTGCGGTATCGATGCGTCGGAACTGATCGATCTCGCGCGCCGTTACGGCGCGACGCGCAAGGCAGCGATCC
GCCTCAATTACGGCATGCAGCGCGTGCGCGGCGGCGGCAACGCGGTGCGCGCGATCGCCAGCCTGCCCGCGCTGACCGGG
GCCTGGCGCGACCGCGCGGGCGGCCTGCTGCTGTCCTCATCGGAATTCGCGCCGATCAATCAGCCCGCGTTGCTGCGCCC
GGATCTGATTCCCGGCTGGCCGCACAAGCTGCCGCGCATCATCAACATGAATGCGATCGGCGATGCGCTGCTGCACCCGG
GCGATGCGGCCTTCGGTCCGAAAATCGAAGCGGTGATCGTCTACAACTCGAATCCCGTTGCCGTTGCACCCGACTCGTCG
AAGGTCGCGGCGGGGTTCGCGCGTGAGGACCTGTTCACGGTGGTTCTCGAGCATTTCAAAACAGATACCGTCGATTTCGC
CGATATTGTGCTGCCGGCGACGACGCAGCTCGAGCACCTGGACGTCCACAAATCGTACGGGCACACCTACGTGATGGCGA
ATCTGCCGGCGATTCCGCCGGTCGGCGAGGCGCGGCCGAACACGGAAATCTTCCGCGGCATCGCGCGCAGCATGGGGCTC
GACGAGCCGGCGCTCTATCACTCCGACGAGGAAGTCGCGCGCGCGGCGCTTCGATGGGACGACCCTTCGCTCGACAGCGA
TTGGGACACGCTGAAGCGCGAAGGCTGGCTGAAGCTGAAATTGCCCGACGCGCCGTTTGCGAGCGGCGGGTTCCGCACGC
CATCCGGCAAGTGCGAGTTCTACAGTGGGCGGCTCGAGCAAATGGGTCTGGATCCGGTGCCCGACCATCTGCCGCCTTTC
GAGTCGGCCGAAGCGGCGCCGGAACTCGCGGCGCGTTACCCGCTCGCGATGATTTCGCCGCCGGCGCGCCACTTCCTGAA
CAGCACGTTCGTCAACGTCGAAAGCCTGCGCAGCACGGAAGGGCAGCCGCACCTCGACATGCACCCGGCGGACGCCGCAT
CGCGCGGCATTGCGGACGGCGATCTCGTGCGCATCTTCAACGACCGCGGATCGATGCAGGCCGTAGCGAGAGTGACGGAC
CGTGCGCGCGCCGGGCTCGTCGTCGGCTTGTCGATCTGGTGGAAGAAGCTGTCGCCCGACGGCTGCAACGCGAACGAAGT
CACGAGCCAGGCGCTGACCGATCTCGGCAATTCGGCGACGTTCTACGACTGTCTGGTCGAAGTCGAACCCGCCTGA

Upstream 100 bases:

>100_bases
CGCCCCATCTTCAAATAAAGCCCGCGACCTGTACGGAATGAGCGCTTTATCCGGCAGGTCCACGTAAAATTTGCGAATCC
CCATCTTCGCACCCACAACA

Downstream 100 bases:

>100_bases
CCGAACGGACGCACGCGCGTCGGCCGAGCATCGTCCCCCGTTCGAAGGGGACGTCTAACCCTGTTGTCTCGCGGCGGGCG
ACCCGCTACGATGCGTTTTA

Product: molybdopterin oxidoreductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 691; Mature: 691

Protein sequence:

>691_residues
MNAATHVARAVCPHDCPDTCAMRVTVENGRAIKVAGDPDHPPTQGVLCTKVSRYAERVHHPDRLTVPLKRIGAKGEGRFA
PISWDEAFDEIGRRLGDIATRAPEAIVPYSYAGTMGLVQGEGIAQRFFHKIGASRLERTICAAAGAAGLRFTYGASVGMH
LEHFEESELILIWGANPIASSLHFWTRAQEAKRRGARLVAIDPYRSLTAEKCHQHIALKPGTDGAFALGMMHVLITENLL
DHDYIADHTLGFDALKARALSYPPERVAQICGIDASELIDLARRYGATRKAAIRLNYGMQRVRGGGNAVRAIASLPALTG
AWRDRAGGLLLSSSEFAPINQPALLRPDLIPGWPHKLPRIINMNAIGDALLHPGDAAFGPKIEAVIVYNSNPVAVAPDSS
KVAAGFAREDLFTVVLEHFKTDTVDFADIVLPATTQLEHLDVHKSYGHTYVMANLPAIPPVGEARPNTEIFRGIARSMGL
DEPALYHSDEEVARAALRWDDPSLDSDWDTLKREGWLKLKLPDAPFASGGFRTPSGKCEFYSGRLEQMGLDPVPDHLPPF
ESAEAAPELAARYPLAMISPPARHFLNSTFVNVESLRSTEGQPHLDMHPADAASRGIADGDLVRIFNDRGSMQAVARVTD
RARAGLVVGLSIWWKKLSPDGCNANEVTSQALTDLGNSATFYDCLVEVEPA

Sequences:

>Translated_691_residues
MNAATHVARAVCPHDCPDTCAMRVTVENGRAIKVAGDPDHPPTQGVLCTKVSRYAERVHHPDRLTVPLKRIGAKGEGRFA
PISWDEAFDEIGRRLGDIATRAPEAIVPYSYAGTMGLVQGEGIAQRFFHKIGASRLERTICAAAGAAGLRFTYGASVGMH
LEHFEESELILIWGANPIASSLHFWTRAQEAKRRGARLVAIDPYRSLTAEKCHQHIALKPGTDGAFALGMMHVLITENLL
DHDYIADHTLGFDALKARALSYPPERVAQICGIDASELIDLARRYGATRKAAIRLNYGMQRVRGGGNAVRAIASLPALTG
AWRDRAGGLLLSSSEFAPINQPALLRPDLIPGWPHKLPRIINMNAIGDALLHPGDAAFGPKIEAVIVYNSNPVAVAPDSS
KVAAGFAREDLFTVVLEHFKTDTVDFADIVLPATTQLEHLDVHKSYGHTYVMANLPAIPPVGEARPNTEIFRGIARSMGL
DEPALYHSDEEVARAALRWDDPSLDSDWDTLKREGWLKLKLPDAPFASGGFRTPSGKCEFYSGRLEQMGLDPVPDHLPPF
ESAEAAPELAARYPLAMISPPARHFLNSTFVNVESLRSTEGQPHLDMHPADAASRGIADGDLVRIFNDRGSMQAVARVTD
RARAGLVVGLSIWWKKLSPDGCNANEVTSQALTDLGNSATFYDCLVEVEPA
>Mature_691_residues
MNAATHVARAVCPHDCPDTCAMRVTVENGRAIKVAGDPDHPPTQGVLCTKVSRYAERVHHPDRLTVPLKRIGAKGEGRFA
PISWDEAFDEIGRRLGDIATRAPEAIVPYSYAGTMGLVQGEGIAQRFFHKIGASRLERTICAAAGAAGLRFTYGASVGMH
LEHFEESELILIWGANPIASSLHFWTRAQEAKRRGARLVAIDPYRSLTAEKCHQHIALKPGTDGAFALGMMHVLITENLL
DHDYIADHTLGFDALKARALSYPPERVAQICGIDASELIDLARRYGATRKAAIRLNYGMQRVRGGGNAVRAIASLPALTG
AWRDRAGGLLLSSSEFAPINQPALLRPDLIPGWPHKLPRIINMNAIGDALLHPGDAAFGPKIEAVIVYNSNPVAVAPDSS
KVAAGFAREDLFTVVLEHFKTDTVDFADIVLPATTQLEHLDVHKSYGHTYVMANLPAIPPVGEARPNTEIFRGIARSMGL
DEPALYHSDEEVARAALRWDDPSLDSDWDTLKREGWLKLKLPDAPFASGGFRTPSGKCEFYSGRLEQMGLDPVPDHLPPF
ESAEAAPELAARYPLAMISPPARHFLNSTFVNVESLRSTEGQPHLDMHPADAASRGIADGDLVRIFNDRGSMQAVARVTD
RARAGLVVGLSIWWKKLSPDGCNANEVTSQALTDLGNSATFYDCLVEVEPA

Specific function: Terminal Reductase During Anaerobic Growth On Various Sulfoxide And N-Oxide Compounds. Allows E.Coli To Grow Anaerobically On Me(2)So As Respiratory Oxidant. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=762, Percent_Identity=28.4776902887139, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI1787870, Length=775, Percent_Identity=28.258064516129, Blast_Score=226, Evalue=4e-60,
Organism=Escherichia coli, GI171474008, Length=767, Percent_Identity=27.2490221642764, Blast_Score=218, Evalue=7e-58,
Organism=Escherichia coli, GI3868721, Length=728, Percent_Identity=26.3736263736264, Blast_Score=188, Evalue=9e-49,
Organism=Escherichia coli, GI1787231, Length=734, Percent_Identity=25.6130790190736, Blast_Score=166, Evalue=7e-42,
Organism=Escherichia coli, GI87081994, Length=752, Percent_Identity=25.1329787234043, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI145693196, Length=725, Percent_Identity=26.2068965517241, Blast_Score=146, Evalue=5e-36,
Organism=Escherichia coli, GI1788534, Length=348, Percent_Identity=22.4137931034483, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI1787778, Length=289, Percent_Identity=25.9515570934256, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI3868720, Length=267, Percent_Identity=25.8426966292135, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI3868719, Length=537, Percent_Identity=21.9739292364991, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 75073; Mature: 75073

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00932 MOLYBDOPTERIN_PROK_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAATHVARAVCPHDCPDTCAMRVTVENGRAIKVAGDPDHPPTQGVLCTKVSRYAERVHH
CCHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCC
PDRLTVPLKRIGAKGEGRFAPISWDEAFDEIGRRLGDIATRAPEAIVPYSYAGTMGLVQG
CCCEEECHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEC
EGIAQRFFHKIGASRLERTICAAAGAAGLRFTYGASVGMHLEHFEESELILIWGANPIAS
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHCCCCCEEEEECCCHHHH
SLHFWTRAQEAKRRGARLVAIDPYRSLTAEKCHQHIALKPGTDGAFALGMMHVLITENLL
HHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCEEECCCCCCHHHHHHHHHHHHHHHH
DHDYIADHTLGFDALKARALSYPPERVAQICGIDASELIDLARRYGATRKAAIRLNYGMQ
CCCHHHHCCCCHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCHH
RVRGGGNAVRAIASLPALTGAWRDRAGGLLLSSSEFAPINQPALLRPDLIPGWPHKLPRI
HHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHCCCE
INMNAIGDALLHPGDAAFGPKIEAVIVYNSNPVAVAPDSSKVAAGFAREDLFTVVLEHFK
EECCHHHHHHCCCCCCCCCCCEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHC
TDTVDFADIVLPATTQLEHLDVHKSYGHTYVMANLPAIPPVGEARPNTEIFRGIARSMGL
CCCCCHHHEEECCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCC
DEPALYHSDEEVARAALRWDDPSLDSDWDTLKREGWLKLKLPDAPFASGGFRTPSGKCEF
CCCHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCEEEEECCCCCCCCCCCCCCCCCEEE
YSGRLEQMGLDPVPDHLPPFESAEAAPELAARYPLAMISPPARHFLNSTFVNVESLRSTE
ECCHHHHCCCCCCCCCCCCCCCHHCCHHHHHHCCHHHCCCHHHHHHHHHHEEHHHHHCCC
GQPHLDMHPADAASRGIADGDLVRIFNDRGSMQAVARVTDRARAGLVVGLSIWWKKLSPD
CCCCCCCCCCHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEHHHHHHHCCCC
GCNANEVTSQALTDLGNSATFYDCLVEVEPA
CCCHHHHHHHHHHHCCCCCEEEEEEEEECCC
>Mature Secondary Structure
MNAATHVARAVCPHDCPDTCAMRVTVENGRAIKVAGDPDHPPTQGVLCTKVSRYAERVHH
CCHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCC
PDRLTVPLKRIGAKGEGRFAPISWDEAFDEIGRRLGDIATRAPEAIVPYSYAGTMGLVQG
CCCEEECHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEC
EGIAQRFFHKIGASRLERTICAAAGAAGLRFTYGASVGMHLEHFEESELILIWGANPIAS
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHCCCCCEEEEECCCHHHH
SLHFWTRAQEAKRRGARLVAIDPYRSLTAEKCHQHIALKPGTDGAFALGMMHVLITENLL
HHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCEEECCCCCCHHHHHHHHHHHHHHHH
DHDYIADHTLGFDALKARALSYPPERVAQICGIDASELIDLARRYGATRKAAIRLNYGMQ
CCCHHHHCCCCHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCHH
RVRGGGNAVRAIASLPALTGAWRDRAGGLLLSSSEFAPINQPALLRPDLIPGWPHKLPRI
HHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHCCCE
INMNAIGDALLHPGDAAFGPKIEAVIVYNSNPVAVAPDSSKVAAGFAREDLFTVVLEHFK
EECCHHHHHHCCCCCCCCCCCEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHC
TDTVDFADIVLPATTQLEHLDVHKSYGHTYVMANLPAIPPVGEARPNTEIFRGIARSMGL
CCCCCHHHEEECCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCC
DEPALYHSDEEVARAALRWDDPSLDSDWDTLKREGWLKLKLPDAPFASGGFRTPSGKCEF
CCCHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCEEEEECCCCCCCCCCCCCCCCCEEE
YSGRLEQMGLDPVPDHLPPFESAEAAPELAARYPLAMISPPARHFLNSTFVNVESLRSTE
ECCHHHHCCCCCCCCCCCCCCCHHCCHHHHHHCCHHHCCCHHHHHHHHHHEEHHHHHCCC
GQPHLDMHPADAASRGIADGDLVRIFNDRGSMQAVARVTDRARAGLVVGLSIWWKKLSPD
CCCCCCCCCCHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEHHHHHHHCCCC
GCNANEVTSQALTDLGNSATFYDCLVEVEPA
CCCHHHHHHHHHHHCCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]