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The map label for this gene is proS [H]

Identifier: 161525965

GI number: 161525965

Start: 3049400

End: 3051136

Strand: Reverse

Name: proS [H]

Synonym: Bmul_2796

Alternate gene names: 161525965

Gene position: 3051136-3049400 (Counterclockwise)

Preceding gene: 161525968

Following gene: 161525964

Centisome position: 88.48

GC content: 65.8

Gene sequence:

>1737_bases
ATGAAAGCTTCCCGTTTCTTTATCGGCACCCTCAAAGAAGCACCGGCCGACGCAGAGATCGTCAGTCACAAGCTGATGGT
GCGCGCCGGCATGATCCGTCGCGTCGCCGGCGGCATCTACAACTATCTGCCGATCGGTCTGCGCTCGATTCGCAAGGTCG
AGGCGATCGTGCGCGAGGAAATGAACCGGGCGGGCGCCGTCGAACTGCTGATGCCGGCCGTGCAGCCGGCCGAACTGTGG
CAGGAGTCAGGTCGCTGGGAGCAGTACGGCCCCGAGCTGCTGCGCTTCAAGGACCGCAAGGACAACGACTTCGTGATCGG
GCCGACGCACGAGGAAGTCGTCACCGACATCGCGCGCAACCAGATCAAGAGCTACCGGCAGATGCCGGTGAACTTCTATC
AGATCCAGACGAAGTTCCGCGACGAGATCCGTCCGCGTTTCGGCGTGATGCGCGGCCGCGAGTTCATCATGAAAGACGCG
TACTCGTTCGACAAGGACGCGGAAGGCCTGCGCGAGTCGTATCGCAAGATGTATGACGCGTACGTGCGCATCTTCACGCG
TCTCGGTCTCGAATTCCGCGCGGTTGCGGCCGACAGCGGCTCGATCGGCGGCAACTTCTCGCACGAGTTCCACGTGATCG
CCGACACCGGCGAGGACGCGATCGCCTACTGCCCGAGCTCCGAGTTCGCGGCGAACGTCGAGGCGGCCGAAGCGCTGCCG
CTGATCGCCGAGCGCGCGGCGCCCGCCGAAGCGCTGACGAAGGTCGCGACGCCGGGCAAGGCGAAGTGCGAGGCCGTCGC
CGAGCTGCTGAACATCCCGCTCGAGCGCACGATCAAGTCGATCGTGCTCGCCACCGACAACGAAGGCGCCGAGCCGACGA
TCTGGCTCGTGATGCTGCGCGGCGATCACGATCTGAACGAGATCAAGGTATCGAAGCTGCCGGGGCTGAAGAACCACCGC
TTCGCGACCGAGCAGGAAATCGTCGAGTGGTTCGGCACGCCGCCCGGCTATCTCGGCCCGATCGGCACGAAGAAGCCGGT
GAAGGTGATCGCCGATCGCACGGTCGCGAACATGAGCGATTTCGTGGTCGGCGCGAACGAGGTCGACTATCACATCGCCG
GCGTGAACTGGGGCCGCGATCTGCCGGAGCCCGAGGTCGCCGATGTGCGCAACGTGAAGAAGGGCGATCCGTCGCCGGAC
GGCAAGGGCGCGATCGACATCTGCCGCGGCATCGAGGTCGGCCACGTGTTCCAGCTCGGCACCAAGTACTCGGAAGCGAT
GGGCGCGACGTTCCTCGACGAATCGGGCAAGCCGCAGCCGATGCTGATGGGCTGCTACGGGATCGGCGTCACGCGTATTC
TCGGCGCGGCGATCGAACAGAACTTCGACGACAAGGGCATCATCTGGCCGGAGTCGATCGCGCCGTTCGAAGTCGTGCTG
TGCCCGATGGGCTACGACCGCAGCGACGCGGTGCGCGAGGCGGCCGACAAGCTGTATGCGGAACTGACGGCGGCCGGCAT
CGACGTGATCCTCGACGATCGCGGCGAGCGTCCGGGCGTGATGTTCGCCGACTGGGAACTGATCGGTGTGCCGCACCGCC
TCGTGATCGGCGAGCGCGGGCTGAAGGACGGCAAGATCGAGTACCAGGGCCGCCGCGACACCGAAGCAACGCTGCTGCCG
GCCGGCGAGGCCGCGGCGACGGTCGCGGAGAAGGTCCGCGCGGCGCTCGCGCACTAA

Upstream 100 bases:

>100_bases
CTACGTCATCTGCGCCATCGTCGTTCGTCGCGCGCAGCTTTTTCACCTTGAATCTTTTTCGGGCGCCCTGCCTGGGGGCG
CCGTTTTTGGAACCGTCCGC

Downstream 100 bases:

>100_bases
GCGGATCGGACGGGGAACGCGGTGGAGTACACCTTCCTGTCGGCTACCGTGCTTCTCGTGCTGATCACCGATCCGCTCGG
CAACATCCCGCTGTTCATTT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRVAGGIYNYLPIGLRSIRKVEAIVREEMNRAGAVELLMPAVQPAELW
QESGRWEQYGPELLRFKDRKDNDFVIGPTHEEVVTDIARNQIKSYRQMPVNFYQIQTKFRDEIRPRFGVMRGREFIMKDA
YSFDKDAEGLRESYRKMYDAYVRIFTRLGLEFRAVAADSGSIGGNFSHEFHVIADTGEDAIAYCPSSEFAANVEAAEALP
LIAERAAPAEALTKVATPGKAKCEAVAELLNIPLERTIKSIVLATDNEGAEPTIWLVMLRGDHDLNEIKVSKLPGLKNHR
FATEQEIVEWFGTPPGYLGPIGTKKPVKVIADRTVANMSDFVVGANEVDYHIAGVNWGRDLPEPEVADVRNVKKGDPSPD
GKGAIDICRGIEVGHVFQLGTKYSEAMGATFLDESGKPQPMLMGCYGIGVTRILGAAIEQNFDDKGIIWPESIAPFEVVL
CPMGYDRSDAVREAADKLYAELTAAGIDVILDDRGERPGVMFADWELIGVPHRLVIGERGLKDGKIEYQGRRDTEATLLP
AGEAAATVAEKVRAALAH

Sequences:

>Translated_578_residues
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRVAGGIYNYLPIGLRSIRKVEAIVREEMNRAGAVELLMPAVQPAELW
QESGRWEQYGPELLRFKDRKDNDFVIGPTHEEVVTDIARNQIKSYRQMPVNFYQIQTKFRDEIRPRFGVMRGREFIMKDA
YSFDKDAEGLRESYRKMYDAYVRIFTRLGLEFRAVAADSGSIGGNFSHEFHVIADTGEDAIAYCPSSEFAANVEAAEALP
LIAERAAPAEALTKVATPGKAKCEAVAELLNIPLERTIKSIVLATDNEGAEPTIWLVMLRGDHDLNEIKVSKLPGLKNHR
FATEQEIVEWFGTPPGYLGPIGTKKPVKVIADRTVANMSDFVVGANEVDYHIAGVNWGRDLPEPEVADVRNVKKGDPSPD
GKGAIDICRGIEVGHVFQLGTKYSEAMGATFLDESGKPQPMLMGCYGIGVTRILGAAIEQNFDDKGIIWPESIAPFEVVL
CPMGYDRSDAVREAADKLYAELTAAGIDVILDDRGERPGVMFADWELIGVPHRLVIGERGLKDGKIEYQGRRDTEATLLP
AGEAAATVAEKVRAALAH
>Mature_578_residues
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRVAGGIYNYLPIGLRSIRKVEAIVREEMNRAGAVELLMPAVQPAELW
QESGRWEQYGPELLRFKDRKDNDFVIGPTHEEVVTDIARNQIKSYRQMPVNFYQIQTKFRDEIRPRFGVMRGREFIMKDA
YSFDKDAEGLRESYRKMYDAYVRIFTRLGLEFRAVAADSGSIGGNFSHEFHVIADTGEDAIAYCPSSEFAANVEAAEALP
LIAERAAPAEALTKVATPGKAKCEAVAELLNIPLERTIKSIVLATDNEGAEPTIWLVMLRGDHDLNEIKVSKLPGLKNHR
FATEQEIVEWFGTPPGYLGPIGTKKPVKVIADRTVANMSDFVVGANEVDYHIAGVNWGRDLPEPEVADVRNVKKGDPSPD
GKGAIDICRGIEVGHVFQLGTKYSEAMGATFLDESGKPQPMLMGCYGIGVTRILGAAIEQNFDDKGIIWPESIAPFEVVL
CPMGYDRSDAVREAADKLYAELTAAGIDVILDDRGERPGVMFADWELIGVPHRLVIGERGLKDGKIEYQGRRDTEATLLP
AGEAAATVAEKVRAALAH

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=241, Percent_Identity=42.7385892116183, Blast_Score=225, Evalue=9e-59,
Organism=Escherichia coli, GI1786392, Length=574, Percent_Identity=56.2717770034843, Blast_Score=691, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=252, Percent_Identity=38.8888888888889, Blast_Score=184, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI193203271, Length=82, Percent_Identity=41.4634146341463, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320931, Length=221, Percent_Identity=40.7239819004525, Blast_Score=194, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24656200, Length=245, Percent_Identity=38.3673469387755, Blast_Score=183, Evalue=3e-46,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63823; Mature: 63823

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRVAGGIYNYLPIGLRSIRKVEAIVREE
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNRAGAVELLMPAVQPAELWQESGRWEQYGPELLRFKDRKDNDFVIGPTHEEVVTDIARN
HHHCCCHHHHHCCCCHHHHHHHCCCHHHCCCHHHHCCCCCCCCEEECCCHHHHHHHHHHH
QIKSYRQMPVNFYQIQTKFRDEIRPRFGVMRGREFIMKDAYSFDKDAEGLRESYRKMYDA
HHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
YVRIFTRLGLEFRAVAADSGSIGGNFSHEFHVIADTGEDAIAYCPSSEFAANVEAAEALP
HHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCEEECCCCHHHCCCHHHHHHH
LIAERAAPAEALTKVATPGKAKCEAVAELLNIPLERTIKSIVLATDNEGAEPTIWLVMLR
HHHHHCCCHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHEEEEECCCCCCCEEEEEEEE
GDHDLNEIKVSKLPGLKNHRFATEQEIVEWFGTPPGYLGPIGTKKPVKVIADRTVANMSD
CCCCCCCEEHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCHH
FVVGANEVDYHIAGVNWGRDLPEPEVADVRNVKKGDPSPDGKGAIDICRGIEVGHVFQLG
HEECCCCCEEEEEEECCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHCCCCCCHHHHHC
TKYSEAMGATFLDESGKPQPMLMGCYGIGVTRILGAAIEQNFDDKGIIWPESIAPFEVVL
CHHHHHHCCHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
CPMGYDRSDAVREAADKLYAELTAAGIDVILDDRGERPGVMFADWELIGVPHRLVIGERG
ECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECEEEECCCCEEEECCCC
LKDGKIEYQGRRDTEATLLPAGEAAATVAEKVRAALAH
CCCCCEEECCCCCCCCEEECCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRVAGGIYNYLPIGLRSIRKVEAIVREE
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNRAGAVELLMPAVQPAELWQESGRWEQYGPELLRFKDRKDNDFVIGPTHEEVVTDIARN
HHHCCCHHHHHCCCCHHHHHHHCCCHHHCCCHHHHCCCCCCCCEEECCCHHHHHHHHHHH
QIKSYRQMPVNFYQIQTKFRDEIRPRFGVMRGREFIMKDAYSFDKDAEGLRESYRKMYDA
HHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
YVRIFTRLGLEFRAVAADSGSIGGNFSHEFHVIADTGEDAIAYCPSSEFAANVEAAEALP
HHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCEEECCCCHHHCCCHHHHHHH
LIAERAAPAEALTKVATPGKAKCEAVAELLNIPLERTIKSIVLATDNEGAEPTIWLVMLR
HHHHHCCCHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHEEEEECCCCCCCEEEEEEEE
GDHDLNEIKVSKLPGLKNHRFATEQEIVEWFGTPPGYLGPIGTKKPVKVIADRTVANMSD
CCCCCCCEEHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCHH
FVVGANEVDYHIAGVNWGRDLPEPEVADVRNVKKGDPSPDGKGAIDICRGIEVGHVFQLG
HEECCCCCEEEEEEECCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHCCCCCCHHHHHC
TKYSEAMGATFLDESGKPQPMLMGCYGIGVTRILGAAIEQNFDDKGIIWPESIAPFEVVL
CHHHHHHCCHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
CPMGYDRSDAVREAADKLYAELTAAGIDVILDDRGERPGVMFADWELIGVPHRLVIGERG
ECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECEEEECCCCEEEECCCC
LKDGKIEYQGRRDTEATLLPAGEAAATVAEKVRAALAH
CCCCCEEECCCCCCCCEEECCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA