Click here to switch to the map view.

The map label for this gene is proB [H]

Identifier: 161525968

GI number: 161525968

Start: 3052734

End: 3053852

Strand: Reverse

Name: proB [H]

Synonym: Bmul_2799

Alternate gene names: 161525968

Gene position: 3053852-3052734 (Counterclockwise)

Preceding gene: 161525969

Following gene: 161525965

Centisome position: 88.56

GC content: 68.63

Gene sequence:

>1119_bases
ATGCGTTCGATCATCGCGGATTCGAAGCGATTGGTAGTGAAAGTAGGCTCCAGCCTCGTGACGAACGACGGCAAGGGGCT
CGATCATGCGGCAATCGGCCGCTGGGCTGCGCAGATCGCGGCGCTGCGCGCGCAGGGCAAGGAAGTCGTGCTCGTCAGTT
CGGGCGCGATCGCCGAAGGCATGCAGCGGCTCGGCTGGAGCAAGCGGCCGCGCGAGATCGACGAGCTGCAGGCCGCGGCC
GCGGTCGGCCAGATGGGGCTTGCGCAGGTCTACGAGAGCCGCTTCACCGAGCACGGCATTCGCACCGCGCAGATCCTGCT
CACGCATGCGGATCTCGCCGATCGCGAGCGCTACCTGAACGCGCGCTCGACGCTGCTGACGCTGCTGCGTCTCGGCGTGG
TGCCGATCATCAACGAGAACGACACAGTCGTCACCGATGAAATCAAGTTCGGCGACAACGATACGCTCGGCGCGCTCGTC
GCGAACCTGATCGAAGGCGACGCGCTGATCATCCTGACCGACCAGTCGGGGCTGTTCACTGCGGATCCGCGCAAGGATCC
GGCGGCGACGCTCGTCGCCGAGGCGAATGCGGGCGCGCCGGAACTCGAGGCGATGGCGGGCGGCGCCGGCTCGAGCCTCG
GCCGCGGCGGGATGCTGACGAAGATTCTCGCGGCGAAGCGCGCCGCGCACAGCGGCGCCAACACGGTGATCGCGAGCGGC
CGCGAGCCGGACGTGCTCGTGCGTCTGGCCGGTGGCGAGGCGATCGGCACGCAACTGATCGCACGCACGGCGCGGATGGC
CGCGCGCAAGCAATGGATGGCCGATCATCTGCAGGTGCGCGGGCACGTCGTGATCGACGCCGGCGCGGTCGAGAAGCTGA
CGGCCGGCGGCAAGAGCCTGCTGCCGATCGGCGTGACGGACGTGCAGGGCGCGTTCGCGCGCGGCGAGGTGATCGCATGC
GTCGGCCCGGACGGGCGCGAAGTCGCGCGCGGCCTGACGAACTACAGCAGCGCCGAGACCAAGCTGATCCACCGCAAGCC
GAGCGGCGAAATCGAAAGCGTGCTCGGCTACATGCTCGAGCCCGAGCTGATCCATCGCGACAACCTGGTGCTCGTCTGA

Upstream 100 bases:

>100_bases
ACGCCTGAGCGTACCCGTCACGCGCCGGTCTGCGTCGGTCCGGCGCCGGAATTGAATCGCGCCCGGCCGGGCAGGCATCA
GATACAGGAGACAGCGCACG

Downstream 100 bases:

>100_bases
ACGCAGCGCGCGCAGTCGCGCCATGCGTTCATCGCCGCCGAAATGAAAAAAGCCCGCTCGATGCGGGCTTTTTGCCGAAC
GCCGCGGACGCTGCCGGCGC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MRSIIADSKRLVVKVGSSLVTNDGKGLDHAAIGRWAAQIAALRAQGKEVVLVSSGAIAEGMQRLGWSKRPREIDELQAAA
AVGQMGLAQVYESRFTEHGIRTAQILLTHADLADRERYLNARSTLLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALV
ANLIEGDALIILTDQSGLFTADPRKDPAATLVAEANAGAPELEAMAGGAGSSLGRGGMLTKILAAKRAAHSGANTVIASG
REPDVLVRLAGGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDAGAVEKLTAGGKSLLPIGVTDVQGAFARGEVIAC
VGPDGREVARGLTNYSSAETKLIHRKPSGEIESVLGYMLEPELIHRDNLVLV

Sequences:

>Translated_372_residues
MRSIIADSKRLVVKVGSSLVTNDGKGLDHAAIGRWAAQIAALRAQGKEVVLVSSGAIAEGMQRLGWSKRPREIDELQAAA
AVGQMGLAQVYESRFTEHGIRTAQILLTHADLADRERYLNARSTLLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALV
ANLIEGDALIILTDQSGLFTADPRKDPAATLVAEANAGAPELEAMAGGAGSSLGRGGMLTKILAAKRAAHSGANTVIASG
REPDVLVRLAGGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDAGAVEKLTAGGKSLLPIGVTDVQGAFARGEVIAC
VGPDGREVARGLTNYSSAETKLIHRKPSGEIESVLGYMLEPELIHRDNLVLV
>Mature_372_residues
MRSIIADSKRLVVKVGSSLVTNDGKGLDHAAIGRWAAQIAALRAQGKEVVLVSSGAIAEGMQRLGWSKRPREIDELQAAA
AVGQMGLAQVYESRFTEHGIRTAQILLTHADLADRERYLNARSTLLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALV
ANLIEGDALIILTDQSGLFTADPRKDPAATLVAEANAGAPELEAMAGGAGSSLGRGGMLTKILAAKRAAHSGANTVIASG
REPDVLVRLAGGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDAGAVEKLTAGGKSLLPIGVTDVQGAFARGEVIAC
VGPDGREVARGLTNYSSAETKLIHRKPSGEIESVLGYMLEPELIHRDNLVLV

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=294, Percent_Identity=37.0748299319728, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI62912457, Length=292, Percent_Identity=37.3287671232877, Blast_Score=145, Evalue=7e-35,
Organism=Escherichia coli, GI1786437, Length=366, Percent_Identity=43.7158469945355, Blast_Score=284, Evalue=7e-78,
Organism=Caenorhabditis elegans, GI17569969, Length=281, Percent_Identity=35.2313167259786, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17569967, Length=281, Percent_Identity=35.2313167259786, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6320506, Length=420, Percent_Identity=36.1904761904762, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6321822, Length=414, Percent_Identity=32.1256038647343, Blast_Score=195, Evalue=1e-50,
Organism=Drosophila melanogaster, GI21357643, Length=350, Percent_Identity=31.7142857142857, Blast_Score=146, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39169; Mature: 39169

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSIIADSKRLVVKVGSSLVTNDGKGLDHAAIGRWAAQIAALRAQGKEVVLVSSGAIAEG
CCCCCCCCCEEEEEECCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHH
MQRLGWSKRPREIDELQAAAAVGQMGLAQVYESRFTEHGIRTAQILLTHADLADRERYLN
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHH
ARSTLLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALVANLIEGDALIILTDQSGLFT
HHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCEE
ADPRKDPAATLVAEANAGAPELEAMAGGAGSSLGRGGMLTKILAAKRAAHSGANTVIASG
CCCCCCCCEEEEEECCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECC
REPDVLVRLAGGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDAGAVEKLTAGGKSL
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCEE
LPIGVTDVQGAFARGEVIACVGPDGREVARGLTNYSSAETKLIHRKPSGEIESVLGYMLE
EEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHEEEECCCCCHHHHHHHHHCC
PELIHRDNLVLV
CHHCCCCCEEEC
>Mature Secondary Structure
MRSIIADSKRLVVKVGSSLVTNDGKGLDHAAIGRWAAQIAALRAQGKEVVLVSSGAIAEG
CCCCCCCCCEEEEEECCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHH
MQRLGWSKRPREIDELQAAAAVGQMGLAQVYESRFTEHGIRTAQILLTHADLADRERYLN
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHH
ARSTLLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALVANLIEGDALIILTDQSGLFT
HHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCEE
ADPRKDPAATLVAEANAGAPELEAMAGGAGSSLGRGGMLTKILAAKRAAHSGANTVIASG
CCCCCCCCEEEEEECCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECC
REPDVLVRLAGGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDAGAVEKLTAGGKSL
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCEE
LPIGVTDVQGAFARGEVIACVGPDGREVARGLTNYSSAETKLIHRKPSGEIESVLGYMLE
EEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHEEEECCCCCHHHHHHHHHCC
PELIHRDNLVLV
CHHCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA