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The map label for this gene is dctA2 [H]

Identifier: 161525909

GI number: 161525909

Start: 2991527

End: 2992885

Strand: Reverse

Name: dctA2 [H]

Synonym: Bmul_2740

Alternate gene names: 161525909

Gene position: 2992885-2991527 (Counterclockwise)

Preceding gene: 161525911

Following gene: 161525908

Centisome position: 86.79

GC content: 64.68

Gene sequence:

>1359_bases
ATGAACAGACAAAACTGCAACCCATCTCGGGAGAGCGCCGTGTCAAAGTTCCTCAATTCGCTGTTTGGTCGAGTCGTGAT
CGCACTGATCCTCGGGGTCGCGCTCGGCGCGTTCTTCCCGCATTTCGCCGAGTCGCTGAAACCGCTCGGCGACGGTTTTC
TGAAGCTCATCAAGATGGTCATCGGCCCGATCGTGTTCTGCGTCGTGGTCAGCGGGATGGCCAATGCGGGCGATCTGAAG
AAGGTCGGCCGCGTCGGCCTGAAGGCCGTCGTCTACTTCGAGGTGATGACGACGCTCGCGCTCGGCATCGGGCTCGTCCT
CGCCTGGCTCACGCGGCCCGGTGAGGGGATGAACATCAACCTGCACTCGCTCGACGCATCGTCGCTCGCGTCGTACGCGA
AGAACGCGGAGAGTCTGAAGGACACGGCCGGCTTCCTGATGAAGATCATCCCCGACACCGCGATCGACGCATTCGCGAAG
GGCGACATCCTGCAGATCCTCGTGTTCGCGGTGCTGTTCGGCTCGGCGCTGTCGCTGCTCGGCGACAAGGCCAAGCGCGT
GAACGGGCTGATCGAGGAGCTGTCGCACGTGTTCTTCCGCGTGATCGGCTTCATCATCAAGCTCGCGCCGCTCGGCGTGC
TCGGCGCGATCGCGTTCACGACCGGCAAGTACGGCGTCGCCTCGCTGAAGCAGCTCGGCTATCTCGTCGCGGTGTTCTAC
GTGAGCTGCGCGGTGTTCGTCACGGTCGTGCTCGGCATCGTGATGCGGCTCGCCGGCTTCTCGGTGTTCAAGCTGATCCG
CTACCTGCGCGAGGAACTGTCGATCGTGCTCGGCACGGCCTCGTCGGACGCGGTGCTGCCGCAGGTGATGCGCAAGCTCG
AATACATGGGCGTCAAGGATTCGACCGTCGGCCTCGTGATCCCGACCGGCTATTCGTTCAACCTCGACGGCTTCTCGATC
TATCTGACGCTCGCGGTGCTGTTCATCGCGCAGGCGACCAACACGCCGCTGTCGGCGCACGATCTGATCGTGGTCCTGAT
CGTGTCGATGATCACGTCGAAGGGCGCGCACGGGATTCCGGGTTCGGCGATCGTGATTCTCGCCGCGACGCTGTCCGCGA
TTCCCGCGATTCCGGTGCTGGGCCTCGTGCTGATCCTGCCGGTCGACTGGTTCGTCGGGATCGCCCGCGCGCTGACGAAC
CTGATCGGCAACTGCGTCGCGACCGTCGTGGTCGCGGTGTGGGAGAACGACATCGACCGCGCCCGTGCGAAGCGCGTGCT
GAACCGCGAGCTGCGCTACGTGCCGGCCGAGGAGGACGGCGCGACGCCGGTCGCCGGCGACCAGGCTCACGCGCTCTGA

Upstream 100 bases:

>100_bases
ACCAACCCGGCCGCGGGGCGGGAAATCCCTATTTTGACGAGCCTTGACGCGACTATATCGGCTTCCTACTATCGACCATA
ACATCACTGGTCTTACCAGT

Downstream 100 bases:

>100_bases
GCGCCGCGCGGCGGTCTGCCCCGGACCGTCCGCGCACGCTGGAACCGCCCGGCGCAGCGATGCGCCGGCATTTCATCAGG
ACGGCGGGCAACGGAGACGG

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MNRQNCNPSRESAVSKFLNSLFGRVVIALILGVALGAFFPHFAESLKPLGDGFLKLIKMVIGPIVFCVVVSGMANAGDLK
KVGRVGLKAVVYFEVMTTLALGIGLVLAWLTRPGEGMNINLHSLDASSLASYAKNAESLKDTAGFLMKIIPDTAIDAFAK
GDILQILVFAVLFGSALSLLGDKAKRVNGLIEELSHVFFRVIGFIIKLAPLGVLGAIAFTTGKYGVASLKQLGYLVAVFY
VSCAVFVTVVLGIVMRLAGFSVFKLIRYLREELSIVLGTASSDAVLPQVMRKLEYMGVKDSTVGLVIPTGYSFNLDGFSI
YLTLAVLFIAQATNTPLSAHDLIVVLIVSMITSKGAHGIPGSAIVILAATLSAIPAIPVLGLVLILPVDWFVGIARALTN
LIGNCVATVVVAVWENDIDRARAKRVLNRELRYVPAEEDGATPVAGDQAHAL

Sequences:

>Translated_452_residues
MNRQNCNPSRESAVSKFLNSLFGRVVIALILGVALGAFFPHFAESLKPLGDGFLKLIKMVIGPIVFCVVVSGMANAGDLK
KVGRVGLKAVVYFEVMTTLALGIGLVLAWLTRPGEGMNINLHSLDASSLASYAKNAESLKDTAGFLMKIIPDTAIDAFAK
GDILQILVFAVLFGSALSLLGDKAKRVNGLIEELSHVFFRVIGFIIKLAPLGVLGAIAFTTGKYGVASLKQLGYLVAVFY
VSCAVFVTVVLGIVMRLAGFSVFKLIRYLREELSIVLGTASSDAVLPQVMRKLEYMGVKDSTVGLVIPTGYSFNLDGFSI
YLTLAVLFIAQATNTPLSAHDLIVVLIVSMITSKGAHGIPGSAIVILAATLSAIPAIPVLGLVLILPVDWFVGIARALTN
LIGNCVATVVVAVWENDIDRARAKRVLNRELRYVPAEEDGATPVAGDQAHAL
>Mature_452_residues
MNRQNCNPSRESAVSKFLNSLFGRVVIALILGVALGAFFPHFAESLKPLGDGFLKLIKMVIGPIVFCVVVSGMANAGDLK
KVGRVGLKAVVYFEVMTTLALGIGLVLAWLTRPGEGMNINLHSLDASSLASYAKNAESLKDTAGFLMKIIPDTAIDAFAK
GDILQILVFAVLFGSALSLLGDKAKRVNGLIEELSHVFFRVIGFIIKLAPLGVLGAIAFTTGKYGVASLKQLGYLVAVFY
VSCAVFVTVVLGIVMRLAGFSVFKLIRYLREELSIVLGTASSDAVLPQVMRKLEYMGVKDSTVGLVIPTGYSFNLDGFSI
YLTLAVLFIAQATNTPLSAHDLIVVLIVSMITSKGAHGIPGSAIVILAATLSAIPAIPVLGLVLILPVDWFVGIARALTN
LIGNCVATVVVAVWENDIDRARAKRVLNRELRYVPAEEDGATPVAGDQAHAL

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=467, Percent_Identity=25.6959314775161, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI4827012, Length=302, Percent_Identity=29.4701986754967, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI21314632, Length=448, Percent_Identity=25, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI40254478, Length=485, Percent_Identity=24.1237113402062, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI5032093, Length=410, Percent_Identity=26.8292682926829, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI223468564, Length=285, Percent_Identity=28.4210526315789, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI223468566, Length=285, Percent_Identity=28.4210526315789, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI66773030, Length=396, Percent_Identity=25.7575757575758, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI262359914, Length=401, Percent_Identity=24.6882793017456, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI194239697, Length=295, Percent_Identity=27.4576271186441, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI301601644, Length=213, Percent_Identity=26.7605633802817, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1789947, Length=416, Percent_Identity=51.6826923076923, Blast_Score=432, Evalue=1e-122,
Organism=Escherichia coli, GI1790514, Length=417, Percent_Identity=38.1294964028777, Blast_Score=281, Evalue=8e-77,
Organism=Escherichia coli, GI1789473, Length=331, Percent_Identity=23.8670694864048, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788024, Length=404, Percent_Identity=21.7821782178218, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17537407, Length=389, Percent_Identity=26.4781491002571, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI71996953, Length=461, Percent_Identity=24.9457700650759, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI193206654, Length=407, Percent_Identity=24.8157248157248, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI193206505, Length=471, Percent_Identity=25.6900212314225, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17541374, Length=406, Percent_Identity=25.3694581280788, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71983106, Length=419, Percent_Identity=24.582338902148, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71983099, Length=419, Percent_Identity=24.582338902148, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24583025, Length=419, Percent_Identity=24.8210023866348, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17137668, Length=419, Percent_Identity=24.8210023866348, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24583023, Length=419, Percent_Identity=24.8210023866348, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI281360481, Length=449, Percent_Identity=26.5033407572383, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI281360483, Length=449, Percent_Identity=26.5033407572383, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17137666, Length=449, Percent_Identity=26.5033407572383, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 48030; Mature: 48030

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRQNCNPSRESAVSKFLNSLFGRVVIALILGVALGAFFPHFAESLKPLGDGFLKLIKMV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGPIVFCVVVSGMANAGDLKKVGRVGLKAVVYFEVMTTLALGIGLVLAWLTRPGEGMNIN
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
LHSLDASSLASYAKNAESLKDTAGFLMKIIPDTAIDAFAKGDILQILVFAVLFGSALSLL
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
GDKAKRVNGLIEELSHVFFRVIGFIIKLAPLGVLGAIAFTTGKYGVASLKQLGYLVAVFY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
VSCAVFVTVVLGIVMRLAGFSVFKLIRYLREELSIVLGTASSDAVLPQVMRKLEYMGVKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
STVGLVIPTGYSFNLDGFSIYLTLAVLFIAQATNTPLSAHDLIVVLIVSMITSKGAHGIP
CCCEEEEECCCEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCC
GSAIVILAATLSAIPAIPVLGLVLILPVDWFVGIARALTNLIGNCVATVVVAVWENDIDR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
ARAKRVLNRELRYVPAEEDGATPVAGDQAHAL
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]