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The map label for this gene is cynR [H]

Identifier: 161525911

GI number: 161525911

Start: 2993785

End: 2994762

Strand: Reverse

Name: cynR [H]

Synonym: Bmul_2742

Alternate gene names: 161525911

Gene position: 2994762-2993785 (Counterclockwise)

Preceding gene: 161525913

Following gene: 161525909

Centisome position: 86.84

GC content: 69.63

Gene sequence:

>978_bases
ATGATGCGGAAATCGAGTCATAGCGTCCAAGACTCGATCCTTATACGATGCATTGGTAAAACCAATACCAGAGCCGCCAT
GCTGCTTCGTCACATCCGCTATTTCCTGGCCGTCGCCGAGCAGGGCAGTTTCACGCGCGCGGCCGATGCGCTGCATGTGT
CGCAGCCGACGCTGTCGCAGCAGATTCGCCAGCTCGAGGAAACGCTCGGCGTGCCGTTGTTCGATCGCTCGGGCCGCACG
GTGCGGCTCACCGAATTCGGCGACGTCTACGCGAAGCACGCGCGCGCGGCGCTGCACGCACTCGACGCGGGCGCGCGTGC
GCTGCACGATGCGGCCGATCTCGCGACCGGCTCGCTGCGGCTCGCGATGATGCCGACCTTCGCCGCCTATCTCAGCGGCT
CGCTCGTCGACGCATTTCATGCCGCGTATCCGAACGTCGTGCTGACGATCGACGCGATGCCGCAGGCGCGCATGGAGTCG
CTGCTCGTCGAAGACCGCCTCGATGCGGGCTTCGCGTTCGTGCCGGCACGCGCGCCGGAGATCGACGCGCTGCCGCTCTG
GGACGAATCGCTCGCGTTCGTGACCGCGCGCACGCATCGGCTCGCGCGCCGTCGCCGCGCGCTCACGCCGGACGAGCTGA
GCGGCGAGCCGCTGGTGCTGCTGAGCCGCGCGTTTGCGACGCGCGAGCGCATCGACCGCTATTTCGTCGAGCATCGCGCG
CAGCCGCGGATCGCGATCGAGACGAACACGGTCAGCGCGGTGCTGGAGCTCGTGCGACGCGGGCGGCTCGCGACCGTGCT
GCCCGATGCGGTCGCGCGCGGCAGCGACGAGCTGTGCGCGCTGCCGGTCGACCCGCCGCTGCCGACGCGCACGGCCGCGC
TGCTCACGCGCAAGGGCGCCTATCGCAGCGTCGCGGCGCGCGCATTCATCGAGTGCGCGCTCGCGTATCGCGTGCCGTCG
GCGGCGTCGAGGACGTAG

Upstream 100 bases:

>100_bases
GACCTCGCGCAGTGCGCACACGAAGCGGCCGAGCGCTACACAGCTGCGCCGGGCCGTCCCGGGCGCGACGGCGAGTGCGT
TGCGCGGAGCGGATGTGCGT

Downstream 100 bases:

>100_bases
CAGCGCTTACGCGCCCGGTGTCGGGAAAAAGATGCCCGCGCGCTGCGCGGCATTCGAGATGTGCGTTTCGATCGCGGTGC
CGGCTGCGGCCGCGTCGCGT

Product: DNA-binding transcriptional regulator CynR

Products: NA

Alternate protein names: Cyn operon transcriptional activator [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MMRKSSHSVQDSILIRCIGKTNTRAAMLLRHIRYFLAVAEQGSFTRAADALHVSQPTLSQQIRQLEETLGVPLFDRSGRT
VRLTEFGDVYAKHARAALHALDAGARALHDAADLATGSLRLAMMPTFAAYLSGSLVDAFHAAYPNVVLTIDAMPQARMES
LLVEDRLDAGFAFVPARAPEIDALPLWDESLAFVTARTHRLARRRRALTPDELSGEPLVLLSRAFATRERIDRYFVEHRA
QPRIAIETNTVSAVLELVRRGRLATVLPDAVARGSDELCALPVDPPLPTRTAALLTRKGAYRSVAARAFIECALAYRVPS
AASRT

Sequences:

>Translated_325_residues
MMRKSSHSVQDSILIRCIGKTNTRAAMLLRHIRYFLAVAEQGSFTRAADALHVSQPTLSQQIRQLEETLGVPLFDRSGRT
VRLTEFGDVYAKHARAALHALDAGARALHDAADLATGSLRLAMMPTFAAYLSGSLVDAFHAAYPNVVLTIDAMPQARMES
LLVEDRLDAGFAFVPARAPEIDALPLWDESLAFVTARTHRLARRRRALTPDELSGEPLVLLSRAFATRERIDRYFVEHRA
QPRIAIETNTVSAVLELVRRGRLATVLPDAVARGSDELCALPVDPPLPTRTAALLTRKGAYRSVAARAFIECALAYRVPS
AASRT
>Mature_325_residues
MMRKSSHSVQDSILIRCIGKTNTRAAMLLRHIRYFLAVAEQGSFTRAADALHVSQPTLSQQIRQLEETLGVPLFDRSGRT
VRLTEFGDVYAKHARAALHALDAGARALHDAADLATGSLRLAMMPTFAAYLSGSLVDAFHAAYPNVVLTIDAMPQARMES
LLVEDRLDAGFAFVPARAPEIDALPLWDESLAFVTARTHRLARRRRALTPDELSGEPLVLLSRAFATRERIDRYFVEHRA
QPRIAIETNTVSAVLELVRRGRLATVLPDAVARGSDELCALPVDPPLPTRTAALLTRKGAYRSVAARAFIECALAYRVPS
AASRT

Specific function: Positively regulates the cynTSX operon, and negatively regulates its own transcription. Binds specifically to the cynR- cynTSX intergenic region [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=288, Percent_Identity=55.2083333333333, Blast_Score=306, Evalue=9e-85,
Organism=Escherichia coli, GI1788887, Length=288, Percent_Identity=30.5555555555556, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790399, Length=278, Percent_Identity=29.136690647482, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1789204, Length=240, Percent_Identity=33.3333333333333, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI145693105, Length=284, Percent_Identity=27.8169014084507, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1788297, Length=255, Percent_Identity=26.6666666666667, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI87081904, Length=289, Percent_Identity=27.3356401384083, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787879, Length=289, Percent_Identity=31.8339100346021, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788481, Length=296, Percent_Identity=26.3513513513513, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787806, Length=288, Percent_Identity=26.3888888888889, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1787530, Length=238, Percent_Identity=28.1512605042017, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788748, Length=285, Percent_Identity=26.3157894736842, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1789173, Length=163, Percent_Identity=33.7423312883436, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790208, Length=260, Percent_Identity=28.0769230769231, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787601, Length=282, Percent_Identity=25.886524822695, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI2367136, Length=248, Percent_Identity=29.4354838709677, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI87082132, Length=298, Percent_Identity=26.1744966442953, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789639, Length=122, Percent_Identity=31.9672131147541, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786401, Length=117, Percent_Identity=32.4786324786325, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790262, Length=222, Percent_Identity=27.027027027027, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1787589, Length=138, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35689; Mature: 35689

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRKSSHSVQDSILIRCIGKTNTRAAMLLRHIRYFLAVAEQGSFTRAADALHVSQPTLSQ
CCCCCCCCHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHH
QIRQLEETLGVPLFDRSGRTVRLTEFGDVYAKHARAALHALDAGARALHDAADLATGSLR
HHHHHHHHHCCCEECCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LAMMPTFAAYLSGSLVDAFHAAYPNVVLTIDAMPQARMESLLVEDRLDAGFAFVPARAPE
EHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCEEEECCCCCC
IDALPLWDESLAFVTARTHRLARRRRALTPDELSGEPLVLLSRAFATRERIDRYFVEHRA
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
QPRIAIETNTVSAVLELVRRGRLATVLPDAVARGSDELCALPVDPPLPTRTAALLTRKGA
CCCEEEECCHHHHHHHHHHCCCEEEECCHHHHCCCCCEEEECCCCCCCCHHHHHHHHCCH
YRSVAARAFIECALAYRVPSAASRT
HHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MMRKSSHSVQDSILIRCIGKTNTRAAMLLRHIRYFLAVAEQGSFTRAADALHVSQPTLSQ
CCCCCCCCHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHH
QIRQLEETLGVPLFDRSGRTVRLTEFGDVYAKHARAALHALDAGARALHDAADLATGSLR
HHHHHHHHHCCCEECCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LAMMPTFAAYLSGSLVDAFHAAYPNVVLTIDAMPQARMESLLVEDRLDAGFAFVPARAPE
EHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCEEEECCCCCC
IDALPLWDESLAFVTARTHRLARRRRALTPDELSGEPLVLLSRAFATRERIDRYFVEHRA
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
QPRIAIETNTVSAVLELVRRGRLATVLPDAVARGSDELCALPVDPPLPTRTAALLTRKGA
CCCEEEECCHHHHHHHHHHCCCEEEECCHHHHCCCCCEEEECCCCCCCCHHHHHHHHCCH
YRSVAARAFIECALAYRVPSAASRT
HHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]