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The map label for this gene is dusB [H]

Identifier: 161525860

GI number: 161525860

Start: 2939437

End: 2940501

Strand: Direct

Name: dusB [H]

Synonym: Bmul_2691

Alternate gene names: 161525860

Gene position: 2939437-2940501 (Clockwise)

Preceding gene: 161525859

Following gene: 161525861

Centisome position: 85.24

GC content: 67.14

Gene sequence:

>1065_bases
ATGCCCGTTATCGGCTCTCACGTATTGCGTAACAACCTGTTCGTCGCGCCGATGGCCGGCGTGACGGACCGTCCGTTCCG
CCAGCTCTGCAAGAAGCTCGGCGCCGGCTACGCGGTGTCCGAGATGGTCGCGTCCAACGCGCAGCTCTGGAAGAGCGCGA
AGACGATGCGTCGCGCGAACCACGCGGGCGAGGTCGAGCCGATCGCGGTGCAGATCGCGGGCGCCGATCCGGCGATGATG
GCCGAAGCGGCGCGCTACAACGTCGACAACGGCGCGCAGATCATCGACATCAACATGGGTTGCCCCGCCAAGAAGGTCTG
CAACGTGGCGGCCGGCTCCGCGCTGCTGCAGAACGAGCCGCTCGTGCAGCGGATCGTCGAGGCCGTCGTCGCGGCCGTCG
GCACGGGGCCCGACGCGGTGCCCGTCACGCTGAAGATCCGCACCGGCTGGGACCGCGAGCACAAGAACGCGATCACGATC
GCGCGGCTCGCCGAGGCCGCCGGCATCTCGATGCTCACCGTGCACGGCCGCACGCGCGCCGATCTGTACCGCGGCGAGGC
CGAATACGAAACGATCGCGGCCGTGAAGGCGGCCGTGCGCATTCCGGTCGTCGCGAACGGCGACATCACGTCGCCCGCGA
AGGCGAAGGCCGTGCTCGAGGCAACGGGCGCCGACGCGCTGATGATCGGTCGTGCCGCGCAAGGCCGGCCGTGGCTGTTC
CGTGAAATCGATCATTTCCTGCAAACGGGCGAGCTGCTGCCGCCGCCGCGCATCGACGAGATCCAGCAGGTGATGAACGA
GCACCTGGAAGACCACTACGCGTTCTACGGCGAATTCACCGGCGTCCGTACTGCGCGCAAGCACATCGGCTGGTACACTC
GCGGCCTTTCCGGTGCCAACGCGTTCCGGCACCGGATGAACACGCTCGATTCCACCCGCGAGCAGCTCGCCGCCGTCAAT
GCGTTCTTCGACGCGCAAAAGGCGCTGTCGGACCACCTCGTCTACGTCGACGACGACGAAAACGGTCAGGGCGAGCCGGA
CGACCATAACCAGTTAGCAGCATGA

Upstream 100 bases:

>100_bases
ACAAACCGGGTCAAATCGGCGGGGGTTGGGAATACGGTAAAATGCGCGTTTTCCCTCCGCCCTTTCGATGCCCGCGCGTG
CCGCGGGCGGTGCCATTGCG

Downstream 100 bases:

>100_bases
GCAAGCACAACATCGAACAATGTGTCCGCGAGAGCCTGGACGTGTATTTCCGGGATCTAGACGGCTCCAATCCGCACGAC
GTCTATGAAATGGTGATGTC

Product: NifR3 family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MPVIGSHVLRNNLFVAPMAGVTDRPFRQLCKKLGAGYAVSEMVASNAQLWKSAKTMRRANHAGEVEPIAVQIAGADPAMM
AEAARYNVDNGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVQRIVEAVVAAVGTGPDAVPVTLKIRTGWDREHKNAITI
ARLAEAAGISMLTVHGRTRADLYRGEAEYETIAAVKAAVRIPVVANGDITSPAKAKAVLEATGADALMIGRAAQGRPWLF
REIDHFLQTGELLPPPRIDEIQQVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGANAFRHRMNTLDSTREQLAAVN
AFFDAQKALSDHLVYVDDDENGQGEPDDHNQLAA

Sequences:

>Translated_354_residues
MPVIGSHVLRNNLFVAPMAGVTDRPFRQLCKKLGAGYAVSEMVASNAQLWKSAKTMRRANHAGEVEPIAVQIAGADPAMM
AEAARYNVDNGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVQRIVEAVVAAVGTGPDAVPVTLKIRTGWDREHKNAITI
ARLAEAAGISMLTVHGRTRADLYRGEAEYETIAAVKAAVRIPVVANGDITSPAKAKAVLEATGADALMIGRAAQGRPWLF
REIDHFLQTGELLPPPRIDEIQQVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGANAFRHRMNTLDSTREQLAAVN
AFFDAQKALSDHLVYVDDDENGQGEPDDHNQLAA
>Mature_353_residues
PVIGSHVLRNNLFVAPMAGVTDRPFRQLCKKLGAGYAVSEMVASNAQLWKSAKTMRRANHAGEVEPIAVQIAGADPAMMA
EAARYNVDNGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVQRIVEAVVAAVGTGPDAVPVTLKIRTGWDREHKNAITIA
RLAEAAGISMLTVHGRTRADLYRGEAEYETIAAVKAAVRIPVVANGDITSPAKAKAVLEATGADALMIGRAAQGRPWLFR
EIDHFLQTGELLPPPRIDEIQQVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGANAFRHRMNTLDSTREQLAAVNA
FFDAQKALSDHLVYVDDDENGQGEPDDHNQLAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=284, Percent_Identity=30.2816901408451, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI239788462, Length=288, Percent_Identity=30.5555555555556, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI31742496, Length=228, Percent_Identity=33.7719298245614, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI40807366, Length=233, Percent_Identity=33.0472103004292, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI8923374, Length=254, Percent_Identity=27.9527559055118, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=57.6323987538941, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1788462, Length=241, Percent_Identity=32.3651452282158, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI145693211, Length=243, Percent_Identity=27.5720164609054, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17543114, Length=286, Percent_Identity=27.972027972028, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI25144369, Length=224, Percent_Identity=33.9285714285714, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17507177, Length=245, Percent_Identity=30.6122448979592, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17510279, Length=255, Percent_Identity=28.2352941176471, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323433, Length=267, Percent_Identity=27.3408239700375, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323560, Length=240, Percent_Identity=30, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323437, Length=231, Percent_Identity=27.2727272727273, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24585320, Length=288, Percent_Identity=29.8611111111111, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24580595, Length=254, Percent_Identity=31.496062992126, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19920448, Length=254, Percent_Identity=31.496062992126, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19921524, Length=230, Percent_Identity=29.5652173913043, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI45549423, Length=255, Percent_Identity=29.0196078431373, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38334; Mature: 38203

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVIGSHVLRNNLFVAPMAGVTDRPFRQLCKKLGAGYAVSEMVASNAQLWKSAKTMRRAN
CCCCCCHHHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
HAGEVEPIAVQIAGADPAMMAEAARYNVDNGAQIIDINMGCPAKKVCNVAAGSALLQNEP
CCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHCCCH
LVQRIVEAVVAAVGTGPDAVPVTLKIRTGWDREHKNAITIARLAEAAGISMLTVHGRTRA
HHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHCCHHHHHHHHHHCCCEEEEECCCCHH
DLYRGEAEYETIAAVKAAVRIPVVANGDITSPAKAKAVLEATGADALMIGRAAQGRPWLF
HHHCCCCHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCHHH
REIDHFLQTGELLPPPRIDEIQQVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGAN
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AFRHRMNTLDSTREQLAAVNAFFDAQKALSDHLVYVDDDENGQGEPDDHNQLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PVIGSHVLRNNLFVAPMAGVTDRPFRQLCKKLGAGYAVSEMVASNAQLWKSAKTMRRAN
CCCCCHHHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
HAGEVEPIAVQIAGADPAMMAEAARYNVDNGAQIIDINMGCPAKKVCNVAAGSALLQNEP
CCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHCCCH
LVQRIVEAVVAAVGTGPDAVPVTLKIRTGWDREHKNAITIARLAEAAGISMLTVHGRTRA
HHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHCCHHHHHHHHHHCCCEEEEECCCCHH
DLYRGEAEYETIAAVKAAVRIPVVANGDITSPAKAKAVLEATGADALMIGRAAQGRPWLF
HHHCCCCHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCHHH
REIDHFLQTGELLPPPRIDEIQQVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGAN
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AFRHRMNTLDSTREQLAAVNAFFDAQKALSDHLVYVDDDENGQGEPDDHNQLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]