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The map label for this gene is nitA [H]

Identifier: 161525849

GI number: 161525849

Start: 2927159

End: 2928082

Strand: Reverse

Name: nitA [H]

Synonym: Bmul_2680

Alternate gene names: 161525849

Gene position: 2928082-2927159 (Counterclockwise)

Preceding gene: 161525851

Following gene: 161525840

Centisome position: 84.91

GC content: 71.1

Gene sequence:

>924_bases
ATGTCCACATCCGTCATCGCCGCATTGCAGCTCGGCGCCTCGCCCGCCGGCACGCGCGCCACGCTCGACACGATCCTCGG
CTACGAAACCGCGATACGCGACAGCGGCGCCTCGCTCGTCGTGCTGCCGGAAGCCGTGCTCGGCGGCTATCCGAAAGGCG
AGATTTTCGGCACGCGGCTCGGCTATCGGCTGCCCGAAGGGCGCGACGCGTATGCGCGCTACGCGGCGCAGGCGATCGAC
GTGCCGGGCCCCGAAACCGACGAGCTCGCCGCGCTGTCGCAGCGCACGGGCGCGAGCCTCGTGGTGGGCGTGATCGAGCG
CGGCGGCAGCACGCTGTACTGCACCGCGCTGTTCTTCGATCCGCGCGACGGGCTCGTCGCGAAGCATCGCAAGCTGATGC
CGACCGGCACCGAGCGGCTGATCTGGGGGCAGGGCGACGGCTCGACGCTGCCGGTCGTCGAGACCGCGGCCGGCCGCGCG
GGCGCCGCGATCTGCTGGGAGAACCACATGCCGCTGCTGCGCTGTGCGATGTATGCGAAGGGCGTGCAGATCTGGTGCGC
GCCGACCGTCGACGAGCGCGATGTCTGGCAAAGCTCGATGCGTCACATCGCGCACGAGGGACGCTGCTTCGTCGTCAGCG
CATGCCAGGTGCAGCCGTCGCCGCGCGCGCTCGGCATCGAGGTGCCGGGCTGGGATCCGGAGCGGCCGCTGATTCGCGGC
GGCAGCGTGATCGTCGGTCCGCTCGGCGATCTGCTGACCGAGCCGCTGGTCGGCGAGGCCGGGCTCGTGAGCGCGCGTAT
CGACACCGATGAACTCGTGCGCGCGCGCTACGACTTCGACGTCGTCGGCCACTATGCGCGCGCCGACGTGTTCTCGCTGC
AGGTGGACGAGCGGCCGAAGCGGCCGGTGGTGTTCGCGGGATGA

Upstream 100 bases:

>100_bases
TATCATCGGCATTCATGTCAGTTATCGTCGTATCGATTTGATCGATGAAATCATATCGATAAAGATGGCGTCATCCCATC
CCGTTACCGGAGACGCTTTC

Downstream 100 bases:

>100_bases
TGCGGGGGCGCCCCCGCACGGGGGCGCCGGGCCGCGAGAATCGACGCGGCCGCGTCAGCGCATCGGCGTTTCCGCGATCC
GCATGTAGTCGGCGATGCGC

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSTSVIAALQLGASPAGTRATLDTILGYETAIRDSGASLVVLPEAVLGGYPKGEIFGTRLGYRLPEGRDAYARYAAQAID
VPGPETDELAALSQRTGASLVVGVIERGGSTLYCTALFFDPRDGLVAKHRKLMPTGTERLIWGQGDGSTLPVVETAAGRA
GAAICWENHMPLLRCAMYAKGVQIWCAPTVDERDVWQSSMRHIAHEGRCFVVSACQVQPSPRALGIEVPGWDPERPLIRG
GSVIVGPLGDLLTEPLVGEAGLVSARIDTDELVRARYDFDVVGHYARADVFSLQVDERPKRPVVFAG

Sequences:

>Translated_307_residues
MSTSVIAALQLGASPAGTRATLDTILGYETAIRDSGASLVVLPEAVLGGYPKGEIFGTRLGYRLPEGRDAYARYAAQAID
VPGPETDELAALSQRTGASLVVGVIERGGSTLYCTALFFDPRDGLVAKHRKLMPTGTERLIWGQGDGSTLPVVETAAGRA
GAAICWENHMPLLRCAMYAKGVQIWCAPTVDERDVWQSSMRHIAHEGRCFVVSACQVQPSPRALGIEVPGWDPERPLIRG
GSVIVGPLGDLLTEPLVGEAGLVSARIDTDELVRARYDFDVVGHYARADVFSLQVDERPKRPVVFAG
>Mature_306_residues
STSVIAALQLGASPAGTRATLDTILGYETAIRDSGASLVVLPEAVLGGYPKGEIFGTRLGYRLPEGRDAYARYAAQAIDV
PGPETDELAALSQRTGASLVVGVIERGGSTLYCTALFFDPRDGLVAKHRKLMPTGTERLIWGQGDGSTLPVVETAAGRAG
AAICWENHMPLLRCAMYAKGVQIWCAPTVDERDVWQSSMRHIAHEGRCFVVSACQVQPSPRALGIEVPGWDPERPLIRGG
SVIVGPLGDLLTEPLVGEAGLVSARIDTDELVRARYDFDVVGHYARADVFSLQVDERPKRPVVFAG

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17557111, Length=306, Percent_Identity=46.7320261437908, Blast_Score=278, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6322027, Length=199, Percent_Identity=64.321608040201, Blast_Score=266, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6322026, Length=111, Percent_Identity=45.045045045045, Blast_Score=101, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000132
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.5.7 [H]

Molecular weight: Translated: 32840; Mature: 32709

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00921 NITRIL_CHT_2 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSVIAALQLGASPAGTRATLDTILGYETAIRDSGASLVVLPEAVLGGYPKGEIFGTRL
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECHHHHCCCCCCCEEEEEC
GYRLPEGRDAYARYAAQAIDVPGPETDELAALSQRTGASLVVGVIERGGSTLYCTALFFD
CCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEHHCCCCEEEEEEEEEC
PRDGLVAKHRKLMPTGTERLIWGQGDGSTLPVVETAAGRAGAAICWENHMPLLRCAMYAK
CCCCHHHHHHCCCCCCCCEEEEECCCCCCCCEEEECCCCCCEEEEECCCCHHHHHHHHHC
GVQIWCAPTVDERDVWQSSMRHIAHEGRCFVVSACQVQPSPRALGIEVPGWDPERPLIRG
CEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEECCCCCCCCCEEEC
GSVIVGPLGDLLTEPLVGEAGLVSARIDTDELVRARYDFDVVGHYARADVFSLQVDERPK
CCEEEECCHHHHCCCCCCCCCEEEEECCHHHHHHHHCCHHHHHHHHHCEEEEEEECCCCC
RPVVFAG
CCEEECC
>Mature Secondary Structure 
STSVIAALQLGASPAGTRATLDTILGYETAIRDSGASLVVLPEAVLGGYPKGEIFGTRL
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECHHHHCCCCCCCEEEEEC
GYRLPEGRDAYARYAAQAIDVPGPETDELAALSQRTGASLVVGVIERGGSTLYCTALFFD
CCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEHHCCCCEEEEEEEEEC
PRDGLVAKHRKLMPTGTERLIWGQGDGSTLPVVETAAGRAGAAICWENHMPLLRCAMYAK
CCCCHHHHHHCCCCCCCCEEEEECCCCCCCCEEEECCCCCCEEEEECCCCHHHHHHHHHC
GVQIWCAPTVDERDVWQSSMRHIAHEGRCFVVSACQVQPSPRALGIEVPGWDPERPLIRG
CEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEECCCCCCCCCEEEC
GSVIVGPLGDLLTEPLVGEAGLVSARIDTDELVRARYDFDVVGHYARADVFSLQVDERPK
CCEEEECCHHHHCCCCCCCCCEEEEECCHHHHHHHHCCHHHHHHHHHCEEEEEEECCCCC
RPVVFAG
CCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400390 [H]