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The map label for this gene is pntB [H]

Identifier: 161525851

GI number: 161525851

Start: 2929176

End: 2930630

Strand: Reverse

Name: pntB [H]

Synonym: Bmul_2682

Alternate gene names: 161525851

Gene position: 2930630-2929176 (Counterclockwise)

Preceding gene: 161525852

Following gene: 161525849

Centisome position: 84.98

GC content: 65.57

Gene sequence:

>1455_bases
ATGAGCATGAACGTCGTCACGCTGCTGTACCTCGTCGCATCGGTCTGCTTCATCCAGGCGCTGAAGGGGCTGTCGAACCC
GAAGAGCGCGCGGCGCGGCAATCTGTTCGGCATGGTCGGGATGGCCATCGCGATCCTCACGACGATCGCGCTGATCGTCA
AGCAGGCCGCGTGGCTCGGCGCGAACCTGCCGCTCGGGCTCGCGCTCGTGCTCGGCGCGCTGGTCGTCGGCGGTGCGGTC
GGCGCGTTCGTCGCCGCGCGCGTCGAGATGACGAAGATGCCGGAACTCGTCGCGGCGATGCACTCGCTGATCGGTCTCGC
GGCCGTCTGCATCGCGTATGCGGTCGTGTCGGAGCCCGAAGCGTTCGGGCTCGTGCCGCAGGACGCCGTGTCGTCGAACT
TCATTCCGTACGGCAACCGCGTCGAGCTGTTCATCGGCACGTTCGTCGGCGCGATCACGTTCTCGGGTTCGGTGATCGCG
TTCGGCAAGCTGTCCGGCAAGTACAAGTTCCGCCTGTTCCAGGGCGCGCCCGTCGTGTACGCAGGCCAGCACCTGATCAA
CCTGATGCTCGCGATCGCGATGCTCGGCTTCGGCATCCTGTTCTTCATCACGCAGGCGTGGCTGCCGTTCATCATCATGA
CGGCGATCGCGTTCGTGCTCGGCGTGCTGATCATCATCCCGATCGGCGGCGCGGACATGCCGGTGGTCGTGTCGATGCTG
AACTCGTACTCGGGCTGGGCCGCGGCCGGCATCGGCTTCTCGCTGAACAACGCGATGCTGATCATCGCGGGTTCGCTCGT
CGGTTCCTCCGGTGCGATCCTGTCGTACATCATGTGCCACGCGATGAACCGCTCGTTCTTCAACGTGATCCTCGGCGGCT
TCGGCGGCGAGGCCGCGGCCGGCGGCGCGGCGGGTGCGCAGGAGCAGCGCCCCGTGAAGTCGGGTTCGGCCGACGATGCG
GCGTTCATGCTCGGCAACGCGGAATCGGTCGTGATCGTGCCGGGCTACGGGCTGGCCGTCGCGCGTGCGCAGCATGCGCT
GAAGGAGCTGACCGACAAGCTGATCGAGAAGGGTGTCGACGTGCGCTACGCGATTCATCCGGTCGCCGGGCGGATGCCGG
GCCACATGAACGTGCTGCTCGCGGAAGCGGAAGTGCCGTACGACATCGTGTTCGAGATGGAGGACATCAACGGCGAGATG
GGGCAGGCGGACGTCGTGCTCGTGCTCGGCGCGAACGACGTCGTGAACCCGGCCGCGAAGAACGATCCGAAATCGCCGAT
CGCGGGCATGCCGATTATCGAGGCGTACAAGGCGCGCACGGTGATCGTCAACAAGCGCTCGATGGCGGCCGGCTACGCGG
GGCTCGACAACGACCTGTTCTACATGGACAAGACGATGATGGTGTTCGGCGACGCGAAGAAGGTCATCGAAGACATGGTG
AAGGCCGTCGAGTAG

Upstream 100 bases:

>100_bases
CCGTCAACGTGTTCGGCGGCTTTCTCGTGACGAGACGGATGCTCGAGATGTTCCGCAAGAAGGAGCCCAAGCGTGTCGAG
GGCGGCAAGGAGGGCGCGCG

Downstream 100 bases:

>100_bases
CGCAGGCGAGCGCTCCGGCCGCGCGATGCGGCCGGATGCGGTTCGACAATGGGGCCCGTCCGGCAGATGCCGGCCGGGCC
CCGGTCGTTTACGGGCGCAC

Product: NAD(P)(+) transhydrogenase

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Proton-translocating transhydrogenase NADP(H)-binding component; Pyridine nucleotide transhydrogenase subunit beta; dIII [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MSMNVVTLLYLVASVCFIQALKGLSNPKSARRGNLFGMVGMAIAILTTIALIVKQAAWLGANLPLGLALVLGALVVGGAV
GAFVAARVEMTKMPELVAAMHSLIGLAAVCIAYAVVSEPEAFGLVPQDAVSSNFIPYGNRVELFIGTFVGAITFSGSVIA
FGKLSGKYKFRLFQGAPVVYAGQHLINLMLAIAMLGFGILFFITQAWLPFIIMTAIAFVLGVLIIIPIGGADMPVVVSML
NSYSGWAAAGIGFSLNNAMLIIAGSLVGSSGAILSYIMCHAMNRSFFNVILGGFGGEAAAGGAAGAQEQRPVKSGSADDA
AFMLGNAESVVIVPGYGLAVARAQHALKELTDKLIEKGVDVRYAIHPVAGRMPGHMNVLLAEAEVPYDIVFEMEDINGEM
GQADVVLVLGANDVVNPAAKNDPKSPIAGMPIIEAYKARTVIVNKRSMAAGYAGLDNDLFYMDKTMMVFGDAKKVIEDMV
KAVE

Sequences:

>Translated_484_residues
MSMNVVTLLYLVASVCFIQALKGLSNPKSARRGNLFGMVGMAIAILTTIALIVKQAAWLGANLPLGLALVLGALVVGGAV
GAFVAARVEMTKMPELVAAMHSLIGLAAVCIAYAVVSEPEAFGLVPQDAVSSNFIPYGNRVELFIGTFVGAITFSGSVIA
FGKLSGKYKFRLFQGAPVVYAGQHLINLMLAIAMLGFGILFFITQAWLPFIIMTAIAFVLGVLIIIPIGGADMPVVVSML
NSYSGWAAAGIGFSLNNAMLIIAGSLVGSSGAILSYIMCHAMNRSFFNVILGGFGGEAAAGGAAGAQEQRPVKSGSADDA
AFMLGNAESVVIVPGYGLAVARAQHALKELTDKLIEKGVDVRYAIHPVAGRMPGHMNVLLAEAEVPYDIVFEMEDINGEM
GQADVVLVLGANDVVNPAAKNDPKSPIAGMPIIEAYKARTVIVNKRSMAAGYAGLDNDLFYMDKTMMVFGDAKKVIEDMV
KAVE
>Mature_483_residues
SMNVVTLLYLVASVCFIQALKGLSNPKSARRGNLFGMVGMAIAILTTIALIVKQAAWLGANLPLGLALVLGALVVGGAVG
AFVAARVEMTKMPELVAAMHSLIGLAAVCIAYAVVSEPEAFGLVPQDAVSSNFIPYGNRVELFIGTFVGAITFSGSVIAF
GKLSGKYKFRLFQGAPVVYAGQHLINLMLAIAMLGFGILFFITQAWLPFIIMTAIAFVLGVLIIIPIGGADMPVVVSMLN
SYSGWAAAGIGFSLNNAMLIIAGSLVGSSGAILSYIMCHAMNRSFFNVILGGFGGEAAAGGAAGAQEQRPVKSGSADDAA
FMLGNAESVVIVPGYGLAVARAQHALKELTDKLIEKGVDVRYAIHPVAGRMPGHMNVLLAEAEVPYDIVFEMEDINGEMG
QADVVLVLGANDVVNPAAKNDPKSPIAGMPIIEAYKARTVIVNKRSMAAGYAGLDNDLFYMDKTMMVFGDAKKVIEDMVK
AVE

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG1282

COG function: function code C; NAD/NADP transhydrogenase beta subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=493, Percent_Identity=45.841784989858, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI122939153, Length=493, Percent_Identity=45.841784989858, Blast_Score=400, Evalue=1e-111,
Organism=Escherichia coli, GI1787886, Length=488, Percent_Identity=50.6147540983607, Blast_Score=454, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17550456, Length=481, Percent_Identity=42.6195426195426, Blast_Score=357, Evalue=1e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012136
- InterPro:   IPR004003 [H]

Pfam domain/function: PF02233 PNTB [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 50663; Mature: 50531

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMNVVTLLYLVASVCFIQALKGLSNPKSARRGNLFGMVGMAIAILTTIALIVKQAAWLG
CCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
ANLPLGLALVLGALVVGGAVGAFVAARVEMTKMPELVAAMHSLIGLAAVCIAYAVVSEPE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
AFGLVPQDAVSSNFIPYGNRVELFIGTFVGAITFSGSVIAFGKLSGKYKFRLFQGAPVVY
HCCCCCCHHHCCCCCCCCCEEEEHHHHHHHHHHCCCCEEEEEECCCEEEEEEEECCCEEE
AGQHLINLMLAIAMLGFGILFFITQAWLPFIIMTAIAFVLGVLIIIPIGGADMPVVVSML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHH
NSYSGWAAAGIGFSLNNAMLIIAGSLVGSSGAILSYIMCHAMNRSFFNVILGGFGGEAAA
HCCCCCCHHCCCEECCCEEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCC
GGAAGAQEQRPVKSGSADDAAFMLGNAESVVIVPGYGLAVARAQHALKELTDKLIEKGVD
CCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCC
VRYAIHPVAGRMPGHMNVLLAEAEVPYDIVFEMEDINGEMGQADVVLVLGANDVVNPAAK
EEEEEECCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCC
NDPKSPIAGMPIIEAYKARTVIVNKRSMAAGYAGLDNDLFYMDKTMMVFGDAKKVIEDMV
CCCCCCCCCCCHHHHHCCEEEEEECHHHHCCCCCCCCCEEEECCEEEEECCHHHHHHHHH
KAVE
HHCC
>Mature Secondary Structure 
SMNVVTLLYLVASVCFIQALKGLSNPKSARRGNLFGMVGMAIAILTTIALIVKQAAWLG
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
ANLPLGLALVLGALVVGGAVGAFVAARVEMTKMPELVAAMHSLIGLAAVCIAYAVVSEPE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
AFGLVPQDAVSSNFIPYGNRVELFIGTFVGAITFSGSVIAFGKLSGKYKFRLFQGAPVVY
HCCCCCCHHHCCCCCCCCCEEEEHHHHHHHHHHCCCCEEEEEECCCEEEEEEEECCCEEE
AGQHLINLMLAIAMLGFGILFFITQAWLPFIIMTAIAFVLGVLIIIPIGGADMPVVVSML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHH
NSYSGWAAAGIGFSLNNAMLIIAGSLVGSSGAILSYIMCHAMNRSFFNVILGGFGGEAAA
HCCCCCCHHCCCEECCCEEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCC
GGAAGAQEQRPVKSGSADDAAFMLGNAESVVIVPGYGLAVARAQHALKELTDKLIEKGVD
CCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCC
VRYAIHPVAGRMPGHMNVLLAEAEVPYDIVFEMEDINGEMGQADVVLVLGANDVVNPAAK
EEEEEECCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCC
NDPKSPIAGMPIIEAYKARTVIVNKRSMAAGYAGLDNDLFYMDKTMMVFGDAKKVIEDMV
CCCCCCCCCCCHHHHHCCEEEEEECHHHHCCCCCCCCCEEEECCEEEEECCHHHHHHHHH
KAVE
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]