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The map label for this gene is ytwF [H]

Identifier: 161525649

GI number: 161525649

Start: 2694467

End: 2694790

Strand: Direct

Name: ytwF [H]

Synonym: Bmul_2480

Alternate gene names: 161525649

Gene position: 2694467-2694790 (Clockwise)

Preceding gene: 161525648

Following gene: 161525651

Centisome position: 78.14

GC content: 66.98

Gene sequence:

>324_bases
ATGCAGATCCTGACGCCCGCGATGCTCGCGGAATGGCTTCGCGATCCGGCGCGCCCCGCGCCGGTCGTCCTCGACGTGCG
CGAACCGTGGGAAGTCGCGACCGCGCAGATCGACGGCAGCGTGTCGATTCCGATGCAGCAGATTCCCGCCCGCAGCGAAG
AGCTCGACGACGAAGCGGAAATCGTCTGCGTCTGCCATCACGGGATGCGCAGCGCGCAAGTCGCGATGTTCCTCGAATCG
CGCGGCTTCACGAAGCTGTACAACCTGCAGGGCGGCATCGACGCGTGGTCGCGCGACGTCGATCCGTCGGTGCCGCGCTA
CTGA

Upstream 100 bases:

>100_bases
AGAAGCAGTATCGCGTCGCGGACGTGTTCGAAACCTACGTCGACCACCTCGTCAACGCGATGGAACCGTCGCGCTTCAAG
TTCTGAGCGGCGGAGCCACC

Downstream 100 bases:

>100_bases
TCGCACGCTACCGATCGCACGCCGGGCACGCGCATCGCGTGCCCGCGCTTCCCTCCTTCGCTAGTCCGTTTCCTTCAGCG
CTTCCGCGACGATCGCAAAC

Product: rhodanese domain-containing protein

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: NA

Number of amino acids: Translated: 107; Mature: 107

Protein sequence:

>107_residues
MQILTPAMLAEWLRDPARPAPVVLDVREPWEVATAQIDGSVSIPMQQIPARSEELDDEAEIVCVCHHGMRSAQVAMFLES
RGFTKLYNLQGGIDAWSRDVDPSVPRY

Sequences:

>Translated_107_residues
MQILTPAMLAEWLRDPARPAPVVLDVREPWEVATAQIDGSVSIPMQQIPARSEELDDEAEIVCVCHHGMRSAQVAMFLES
RGFTKLYNLQGGIDAWSRDVDPSVPRY
>Mature_107_residues
MQILTPAMLAEWLRDPARPAPVVLDVREPWEVATAQIDGSVSIPMQQIPARSEELDDEAEIVCVCHHGMRSAQVAMFLES
RGFTKLYNLQGGIDAWSRDVDPSVPRY

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 11978; Mature: 11978

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQILTPAMLAEWLRDPARPAPVVLDVREPWEVATAQIDGSVSIPMQQIPARSEELDDEAE
CCCCCHHHHHHHHHCCCCCCCEEEECCCCCCEEEEECCCCCCCCHHHCCCCCCCCCCCCC
IVCVCHHGMRSAQVAMFLESRGFTKLYNLQGGIDAWSRDVDPSVPRY
EEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQILTPAMLAEWLRDPARPAPVVLDVREPWEVATAQIDGSVSIPMQQIPARSEELDDEAE
CCCCCHHHHHHHHHCCCCCCCEEEECCCCCCEEEEECCCCCCCCHHHCCCCCCCCCCCCC
IVCVCHHGMRSAQVAMFLESRGFTKLYNLQGGIDAWSRDVDPSVPRY
EEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]