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The map label for this gene is pcm1 [H]

Identifier: 161525648

GI number: 161525648

Start: 2693799

End: 2694452

Strand: Direct

Name: pcm1 [H]

Synonym: Bmul_2479

Alternate gene names: 161525648

Gene position: 2693799-2694452 (Clockwise)

Preceding gene: 161525647

Following gene: 161525649

Centisome position: 78.12

GC content: 64.68

Gene sequence:

>654_bases
ATGAATATCGAAAAAGCGCGTTTCAACATGATCGAACAGCAGATCCGGCCGTGGGACGTGCTGGATCTGGACGTCCTGGG
CCTGCTGTCGATCGTCAAGCGTGAGAATTTCGTTCCGGCCGAATACCGCGATCTCGCGTTCGCCGATCTTGAACTGCCGC
TGCCCGGCGGCCGCAAGATGCTGTTCCCGCGCGTCGAAGCGCGCGTGCTGCAGGAACTGGCCGTCAAGAAGCACGAGAAC
GTGCTCGTGATCGGCGCGGGCTCCGGCTACCTGACCGCGCTGATCGCACACCGCGCGCTGCACGTCACGGCGGTCGAGAT
CGACCCGACGATCGCGAAGTTCGCGGCCGACAACCTGCGCGAGAACGCCGTGACGAACGCGGAAGTCGTGCTCGGCGACG
GTTCGCTCGGCTGGCCCGACAAGGCGCCGTACGACGTGATCTGCGTCGCGGGCGGCCTGCCGGTCGTGCCGCAGCAGATG
CTCGAACAGCTGAAGATCGGCGGCCGCCTGTCGGCGTTCGTCGGCGGCCGGCCGGTAATGAAGGCGCAGGTGATCACGCG
CATCGACGAGAAGCAGTATCGCGTCGCGGACGTGTTCGAAACCTACGTCGACCACCTCGTCAACGCGATGGAACCGTCGC
GCTTCAAGTTCTGA

Upstream 100 bases:

>100_bases
TTCGTTGCAGGCCGCCTGAAGGCTTGCCGGGCGCCCCGTTTGGTACAATCACGAAGTTTTTCTATCCTCGCTGATAGCCG
CCAGGCTGCATGACCACACC

Downstream 100 bases:

>100_bases
GCGGCGGAGCCACCATGCAGATCCTGACGCCCGCGATGCTCGCGGAATGGCTTCGCGATCCGGCGCGCCCCGCGCCGGTC
GTCCTCGACGTGCGCGAACC

Product: protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase 1; Protein L-isoaspartyl methyltransferase 1; Protein-beta-aspartate methyltransferase 1; PIMT 1 [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MNIEKARFNMIEQQIRPWDVLDLDVLGLLSIVKRENFVPAEYRDLAFADLELPLPGGRKMLFPRVEARVLQELAVKKHEN
VLVIGAGSGYLTALIAHRALHVTAVEIDPTIAKFAADNLRENAVTNAEVVLGDGSLGWPDKAPYDVICVAGGLPVVPQQM
LEQLKIGGRLSAFVGGRPVMKAQVITRIDEKQYRVADVFETYVDHLVNAMEPSRFKF

Sequences:

>Translated_217_residues
MNIEKARFNMIEQQIRPWDVLDLDVLGLLSIVKRENFVPAEYRDLAFADLELPLPGGRKMLFPRVEARVLQELAVKKHEN
VLVIGAGSGYLTALIAHRALHVTAVEIDPTIAKFAADNLRENAVTNAEVVLGDGSLGWPDKAPYDVICVAGGLPVVPQQM
LEQLKIGGRLSAFVGGRPVMKAQVITRIDEKQYRVADVFETYVDHLVNAMEPSRFKF
>Mature_217_residues
MNIEKARFNMIEQQIRPWDVLDLDVLGLLSIVKRENFVPAEYRDLAFADLELPLPGGRKMLFPRVEARVLQELAVKKHEN
VLVIGAGSGYLTALIAHRALHVTAVEIDPTIAKFAADNLRENAVTNAEVVLGDGSLGWPDKAPYDVICVAGGLPVVPQQM
LEQLKIGGRLSAFVGGRPVMKAQVITRIDEKQYRVADVFETYVDHLVNAMEPSRFKF

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI226530908, Length=141, Percent_Identity=36.1702127659575, Blast_Score=67, Evalue=9e-12,
Organism=Escherichia coli, GI1789100, Length=169, Percent_Identity=34.3195266272189, Blast_Score=93, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 24116; Mature: 24116

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS01279 PCMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIEKARFNMIEQQIRPWDVLDLDVLGLLSIVKRENFVPAEYRDLAFADLELPLPGGRKM
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHCCEEEEEECCCCCCCEE
LFPRVEARVLQELAVKKHENVLVIGAGSGYLTALIAHRALHVTAVEIDPTIAKFAADNLR
ECHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHEEEEEEECHHHHHHHHHHHH
ENAVTNAEVVLGDGSLGWPDKAPYDVICVAGGLPVVPQQMLEQLKIGGRLSAFVGGRPVM
HHCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEHHCCCCHH
KAQVITRIDEKQYRVADVFETYVDHLVNAMEPSRFKF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNIEKARFNMIEQQIRPWDVLDLDVLGLLSIVKRENFVPAEYRDLAFADLELPLPGGRKM
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHCCEEEEEECCCCCCCEE
LFPRVEARVLQELAVKKHENVLVIGAGSGYLTALIAHRALHVTAVEIDPTIAKFAADNLR
ECHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHEEEEEEECHHHHHHHHHHHH
ENAVTNAEVVLGDGSLGWPDKAPYDVICVAGGLPVVPQQMLEQLKIGGRLSAFVGGRPVM
HHCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEHHCCCCHH
KAQVITRIDEKQYRVADVFETYVDHLVNAMEPSRFKF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA