Click here to switch to the map view.

The map label for this gene is norM [H]

Identifier: 161525382

GI number: 161525382

Start: 2426731

End: 2428119

Strand: Direct

Name: norM [H]

Synonym: Bmul_2212

Alternate gene names: 161525382

Gene position: 2426731-2428119 (Clockwise)

Preceding gene: 161525381

Following gene: 161525383

Centisome position: 70.37

GC content: 70.55

Gene sequence:

>1389_bases
ATGTCGCATTCCGGTCTTACGCGGACGGCCGCCGCGCCGCCGTCCCTGTCCCGTCACGCCGCCGATACCGCGCGCCTCGC
CGCACCGCTCGCGATCGCACAGCTCTCGCAGATGGCGATGAGCGTCACCGACACGGTGCTGCTCGGCTCGCTCGGCCCCG
ATTCGCTCGCGGCCGGCGGCCTCGGCGCGAACTTCTTCTTCGTGATCGTCACCGTGCTGCAGGGTGTGCTGTCGTCGGTC
AGCGTCAGCGTCGCGCATGCGCGCGGCGCGCAGGCCGACGACCGCGTGCCGCACATCTACTGGACCGGCTTCGTGCTGTC
GGTACTGCTCGCGATTCCGGCGATCGTCGCGCTGTCGCTCGCCGAACCGATCCTGCTGATGTTCCACGAACCGCCGGCGC
TCGCGCATCACGTCGGCCAGTACACGGGCATCCTGCGCTTCGCGGCGCTCGGCAGCCTGATCGGCGTCGGGCTCATGCGC
GCGTTCCTGCCTGCGATCGGCGCGGCACGCCGCTTGCTGTGGGTGTCGATCGGCGGCGTCGGCGTGAACGGCGTGCTGAA
CTACGGACTCATTCATGGCGCATTCGGGCTGCCGCGGCTCGGCTTTCTCGGTTCGGCCGTCGCGACGACGATCACGATCT
GGCTGACGGCGCTCGCGCTGATCTGGCTGCTGCACGGGCGCCAGCGCTTTCGCCATTTCGTGACGGCCGCGCGCCCGAAG
CTGCCGATGATGGGCGAGCTGATCGGGATCGGCTGGCCCGTCGCGATCACCTATGGGGTCGAATCGACGCTGTTCCTCGC
GACCGGGCTGACGGTCGGCGTACTCGGTGCGACGTCGCTCGCCGCGCACCAGATCGCGCTGAACGTCGCATCGGTGTCGT
TCATGGTGCCGCTCGCGATCGGCCAGGCGGCGAACGTGCGCGTCGGCTACTGGGTCGGCGCCGGCGAACCGGTCGCCGCA
CGCCATGCGGGCTTCGTCGCGCTCGGGCTCGGCGTCGCGTTCATGTCGCTGTCCGGCCTCGTGATGATCGTCGCGCCGCA
CACGATCGTCGGGCTGTACCTGCATCTCGACGATCCGGCGAACGCGGGCACGGTCGCGCTCGCCGCGTCGCTGCTCGGCA
TCGCCGCGGTGTTCCAGATCGTCGACGGGATGCAGACCGTCGCGTCGGGCGCGTTGCGCGGGCTCAAGGACACGCGCATC
CCGATGCTCGCGGCAACCTTCGGCTACTGGGGAATCGGCTTTCCGACCGGCTACTGGCTCGCGTTCCATGCCGGACTCGG
CGCGCGCGGCCTGTGGTGGGGGCTCGCGGCCGGCCTCGCGAGCGTCGCCGCGCTGATGGCATGGCGCTTCCATCTGAAGA
GCGCGTCGCTGGCGAGCGCCGCGCGCTGA

Upstream 100 bases:

>100_bases
GCCGCGCACGTTCGACACCGTGCGAATAAAGCCGTAAGCTAGCCGTCTCTCAAAATAAATACAGGCAGCGCGGGCCCGCA
CTCGCGCGAGCGCCCCCTCC

Downstream 100 bases:

>100_bases
GCGCGACACGACGCGAAATCGCGGCCGGCGCTATGCTTGTCGCCTGAACACCCACACGCCTCACGATACGCCCTCGCCGC
GCGCCGCGCGGCCTTCCCCT

Product: MATE efflux family protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSHSGLTRTAAAPPSLSRHAADTARLAAPLAIAQLSQMAMSVTDTVLLGSLGPDSLAAGGLGANFFFVIVTVLQGVLSSV
SVSVAHARGAQADDRVPHIYWTGFVLSVLLAIPAIVALSLAEPILLMFHEPPALAHHVGQYTGILRFAALGSLIGVGLMR
AFLPAIGAARRLLWVSIGGVGVNGVLNYGLIHGAFGLPRLGFLGSAVATTITIWLTALALIWLLHGRQRFRHFVTAARPK
LPMMGELIGIGWPVAITYGVESTLFLATGLTVGVLGATSLAAHQIALNVASVSFMVPLAIGQAANVRVGYWVGAGEPVAA
RHAGFVALGLGVAFMSLSGLVMIVAPHTIVGLYLHLDDPANAGTVALAASLLGIAAVFQIVDGMQTVASGALRGLKDTRI
PMLAATFGYWGIGFPTGYWLAFHAGLGARGLWWGLAAGLASVAALMAWRFHLKSASLASAAR

Sequences:

>Translated_462_residues
MSHSGLTRTAAAPPSLSRHAADTARLAAPLAIAQLSQMAMSVTDTVLLGSLGPDSLAAGGLGANFFFVIVTVLQGVLSSV
SVSVAHARGAQADDRVPHIYWTGFVLSVLLAIPAIVALSLAEPILLMFHEPPALAHHVGQYTGILRFAALGSLIGVGLMR
AFLPAIGAARRLLWVSIGGVGVNGVLNYGLIHGAFGLPRLGFLGSAVATTITIWLTALALIWLLHGRQRFRHFVTAARPK
LPMMGELIGIGWPVAITYGVESTLFLATGLTVGVLGATSLAAHQIALNVASVSFMVPLAIGQAANVRVGYWVGAGEPVAA
RHAGFVALGLGVAFMSLSGLVMIVAPHTIVGLYLHLDDPANAGTVALAASLLGIAAVFQIVDGMQTVASGALRGLKDTRI
PMLAATFGYWGIGFPTGYWLAFHAGLGARGLWWGLAAGLASVAALMAWRFHLKSASLASAAR
>Mature_461_residues
SHSGLTRTAAAPPSLSRHAADTARLAAPLAIAQLSQMAMSVTDTVLLGSLGPDSLAAGGLGANFFFVIVTVLQGVLSSVS
VSVAHARGAQADDRVPHIYWTGFVLSVLLAIPAIVALSLAEPILLMFHEPPALAHHVGQYTGILRFAALGSLIGVGLMRA
FLPAIGAARRLLWVSIGGVGVNGVLNYGLIHGAFGLPRLGFLGSAVATTITIWLTALALIWLLHGRQRFRHFVTAARPKL
PMMGELIGIGWPVAITYGVESTLFLATGLTVGVLGATSLAAHQIALNVASVSFMVPLAIGQAANVRVGYWVGAGEPVAAR
HAGFVALGLGVAFMSLSGLVMIVAPHTIVGLYLHLDDPANAGTVALAASLLGIAAVFQIVDGMQTVASGALRGLKDTRIP
MLAATFGYWGIGFPTGYWLAFHAGLGARGLWWGLAAGLASVAALMAWRFHLKSASLASAAR

Specific function: Multidrug efflux pump. Confers probably resistance to the cationic peptide polymyxin B (PMB) [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Homo sapiens, GI22907060, Length=427, Percent_Identity=27.4004683840749, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI153792564, Length=451, Percent_Identity=24.6119733924612, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI38261960, Length=476, Percent_Identity=23.109243697479, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI48994890, Length=435, Percent_Identity=32.6436781609195, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI226510954, Length=451, Percent_Identity=26.1640798226164, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 47678; Mature: 47547

Theoretical pI: Translated: 10.88; Mature: 10.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSGLTRTAAAPPSLSRHAADTARLAAPLAIAQLSQMAMSVTDTVLLGSLGPDSLAAGG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC
LGANFFFVIVTVLQGVLSSVSVSVAHARGAQADDRVPHIYWTGFVLSVLLAIPAIVALSL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
AEPILLMFHEPPALAHHVGQYTGILRFAALGSLIGVGLMRAFLPAIGAARRLLWVSIGGV
HCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCC
GVNGVLNYGLIHGAFGLPRLGFLGSAVATTITIWLTALALIWLLHGRQRFRHFVTAARPK
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LPMMGELIGIGWPVAITYGVESTLFLATGLTVGVLGATSLAAHQIALNVASVSFMVPLAI
CCCHHHHHCCCCCEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GQAANVRVGYWVGAGEPVAARHAGFVALGLGVAFMSLSGLVMIVAPHTIVGLYLHLDDPA
CCCCCEEEEEEECCCCCHHHHHCCHHHHHHHHHHHHHCCEEEEEECCEEEEEEEEECCCC
NAGTVALAASLLGIAAVFQIVDGMQTVASGALRGLKDTRIPMLAATFGYWGIGFPTGYWL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHH
AFHAGLGARGLWWGLAAGLASVAALMAWRFHLKSASLASAAR
HEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCC
>Mature Secondary Structure 
SHSGLTRTAAAPPSLSRHAADTARLAAPLAIAQLSQMAMSVTDTVLLGSLGPDSLAAGG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC
LGANFFFVIVTVLQGVLSSVSVSVAHARGAQADDRVPHIYWTGFVLSVLLAIPAIVALSL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
AEPILLMFHEPPALAHHVGQYTGILRFAALGSLIGVGLMRAFLPAIGAARRLLWVSIGGV
HCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCC
GVNGVLNYGLIHGAFGLPRLGFLGSAVATTITIWLTALALIWLLHGRQRFRHFVTAARPK
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LPMMGELIGIGWPVAITYGVESTLFLATGLTVGVLGATSLAAHQIALNVASVSFMVPLAI
CCCHHHHHCCCCCEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GQAANVRVGYWVGAGEPVAARHAGFVALGLGVAFMSLSGLVMIVAPHTIVGLYLHLDDPA
CCCCCEEEEEEECCCCCHHHHHCCHHHHHHHHHHHHHCCEEEEEECCEEEEEEEEECCCC
NAGTVALAASLLGIAAVFQIVDGMQTVASGALRGLKDTRIPMLAATFGYWGIGFPTGYWL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHH
AFHAGLGARGLWWGLAAGLASVAALMAWRFHLKSASLASAAR
HEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12399047 [H]