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The map label for this gene is relA [H]

Identifier: 161524953

GI number: 161524953

Start: 1969167

End: 1971401

Strand: Reverse

Name: relA [H]

Synonym: Bmul_1780

Alternate gene names: 161524953

Gene position: 1971401-1969167 (Counterclockwise)

Preceding gene: 161524954

Following gene: 161524952

Centisome position: 57.17

GC content: 68.9

Gene sequence:

>2235_bases
ATGACCGAGTCCGTATCCGCTTCCTCCGTCGCGGCGCCGTCGTTCGACGACGTGCTCGCGTTCGTGCGCGAGCGGGCCGG
CGACGCGTGTCTCGCGTCGGGCGAGCGGCTTGCCGATCACGCGGCCGGCACCGCGGCGATCATGCGCACGCTGAATGTCG
ATCCGCCCGCGATGCAGGCGGCCGCGCTGTTCGCGCTGACGCCGCATCTGAGCGACCCCGAACGCGAACTGACGGAGCGC
TTCGGCGACGAAGTCGCGCGGCTCGTGGCCGACGTGCGCAAGCTGTTGCGGCTCGGCACTGTCAGCCTGCGCGCGGCGCA
GCACGCGGCGCCCGAGGCGGGCCGCGATGCGGCCCAGCAGCGGCGCGCGCAGATCGAGGCGCTGCGCAAGATGCTGCTCG
CGTTCGCGCAGGACATCCGCGTCGTGCTGATCCGGCTCGCGTCGCGGCTGCAGTCGCTGCGCTACTACGCGGCGGCGAAG
CTGGAACCGCCGCCGGAAGTCGCGCGCGAGACGCTCGAGATCTATGCGCCGCTCGCGAACCGCCTCGGCATCTGGCAACT
GAAGTGGGAGCTCGAGGATCTCGCGTTCCGCTTCGAGGATCCGATCACCTACAAGCGCATCGCCAAGCTGCTCGACGAGA
AGCGCGCGGAGCGCGAGGCGTACGTCGCGCAGGCGATCGAGCGGCTGCAGCAGGAACTCGCCGCCGCGAACGTGCAGGCC
GACGTCAGCGGCCGGCCGAAGCACATCTACAGCATCTGGCGCAAGATGCGCGGCAAGGAGCTCGATTTTTCCGAGCTGTA
CGACGTGCGTGCGTTCCGCGTGATCGTGCCGGACATCAAGGATTGCTACGCGGTGCTCGGCATCGTGCACCATCTGTGGC
AGCCGGTGCCGAAGGAATTCGACGACTATATCTCGCGACCGAAGCCGAACGGCTACAAGTCGCTGCATACGGTCGTGATC
GGCGACGACGGCCGCGCCTTCGAGGTACAGATCCGCACGCAGGAAATGCATCGCTTTGCCGAATACGGCGTGGCCGCGCA
CTGGCGCTACAAGGAGGCCGGCACGCGCGGCTACGGCGGCCAGTTCTCGGCGAGCGACAAGTACGACGAGAAGATCGCGT
GGCTGCGTCAGCTGCTCGCGTGGAAGGACGACGTCGAGGATGGCGCCGAGCAGTCGGGCGACAAGGCGTGGGCGCAGCTG
CGCGAGACCTCGCTCGACGATGACCACATCTACGTGCTGACGCCGCAGGCGCGCGTGATCGCGCTGCCGCAGGGCGCGAC
GCCGGTCGACTTCGCGTATCACCTGCACAGCGAACTCGGCCATCGCTGCCGCGGCGCGCGCGTCGACGGCGCGATGGTGC
CGCTCAACACGCCGCTCGCGAACGGCCAGACGGTCGAGATCGTCGCGGTGAAGGAGGGCGGCCCGTCGCGCGACTGGCTC
AACCCGCAGCTCGGCTATCTGAAGAGCCCGCGTGCACGCCAGAAGGTGCGCGCGTGGTTCAACGCGATCGAGCAGGAAGA
GAACATCGCGCACGGTCGTGCGCTCGTCGAAAAGACGCTGCAGCGCGAAGGCAAGACGTCGGTGAGTCTCGACAGCCTTG
CCGCGAAGCTCGGCTTCAAGTCGCCCGAAGAGCTGTTCTCGGTCGTCGGCAAGGAAGAGTTCAGCCTGCGCAACATCGAG
CAGGCATTGTCCGACGCGCCGCCCGCCGAGCCGGCGCCCGAGGCGCCCGCCGATTTCGAGAAGCGCAGCAGCGGCGCGAG
CGTCGCGCACGGCGCCTCGACGGGCGTGCTCGTGGTCGGCGTCGATGCGCTGCTCACGCAGCTCGCGCGCTGTTGCCGGC
CGGCGCCGCCGGATCCGATCAGCGGGTTCGTCACGCGCGGCAAGGGCATGTCGATTCACCGCAGCGACTGCGCGACGTTC
CGCCGGATGGCCGAGCGCGCGCCCGAACGCGTGCTGCACACCACGTGGTCGGCCGACGTGCTCGGCGGCCGCGGCGCGTC
CGTCTATCCGGTCGACCTGATGATCGAGGCGACCGATCGGCAGGGCTTGCTGCGCGACATTTCCGAAGTCTTCGCGCGCG
AGAAGATGAACGTGGTCGGCGTGAAGACGCAGAGCCGCCGCAATACAGCCTTCATGCAGTTCACGGTCGAGGTGTCGAAT
TCGGCGCAGGTGCAGCGCGCCTGCACGCTGCTCGGCGAAGTGCAGGGCGTCGTGCGGGCGGGGCGTAAGGCGTAA

Upstream 100 bases:

>100_bases
GCGAAGCTCGCCGATCCGGCGCTGCTCGTCGAGATCGTGGTCGTCGCCGCGCAGCGGAGCTGATCCGCGTCGAGATGACC
GTATTCCGTCAGGCCGTGCG

Downstream 100 bases:

>100_bases
GGCGGCGATCACGCGAAACGGGGCGCGATCGCTTGCTGTTTCGGTTCACCGCTACGGTTCACCGCTACGGCTTGCCGCTC
CGGCCGCCGCCGCGGTTTTC

Product: (p)ppGpp synthetase I SpoT/RelA

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I [H]

Number of amino acids: Translated: 744; Mature: 743

Protein sequence:

>744_residues
MTESVSASSVAAPSFDDVLAFVRERAGDACLASGERLADHAAGTAAIMRTLNVDPPAMQAAALFALTPHLSDPERELTER
FGDEVARLVADVRKLLRLGTVSLRAAQHAAPEAGRDAAQQRRAQIEALRKMLLAFAQDIRVVLIRLASRLQSLRYYAAAK
LEPPPEVARETLEIYAPLANRLGIWQLKWELEDLAFRFEDPITYKRIAKLLDEKRAEREAYVAQAIERLQQELAAANVQA
DVSGRPKHIYSIWRKMRGKELDFSELYDVRAFRVIVPDIKDCYAVLGIVHHLWQPVPKEFDDYISRPKPNGYKSLHTVVI
GDDGRAFEVQIRTQEMHRFAEYGVAAHWRYKEAGTRGYGGQFSASDKYDEKIAWLRQLLAWKDDVEDGAEQSGDKAWAQL
RETSLDDDHIYVLTPQARVIALPQGATPVDFAYHLHSELGHRCRGARVDGAMVPLNTPLANGQTVEIVAVKEGGPSRDWL
NPQLGYLKSPRARQKVRAWFNAIEQEENIAHGRALVEKTLQREGKTSVSLDSLAAKLGFKSPEELFSVVGKEEFSLRNIE
QALSDAPPAEPAPEAPADFEKRSSGASVAHGASTGVLVVGVDALLTQLARCCRPAPPDPISGFVTRGKGMSIHRSDCATF
RRMAERAPERVLHTTWSADVLGGRGASVYPVDLMIEATDRQGLLRDISEVFAREKMNVVGVKTQSRRNTAFMQFTVEVSN
SAQVQRACTLLGEVQGVVRAGRKA

Sequences:

>Translated_744_residues
MTESVSASSVAAPSFDDVLAFVRERAGDACLASGERLADHAAGTAAIMRTLNVDPPAMQAAALFALTPHLSDPERELTER
FGDEVARLVADVRKLLRLGTVSLRAAQHAAPEAGRDAAQQRRAQIEALRKMLLAFAQDIRVVLIRLASRLQSLRYYAAAK
LEPPPEVARETLEIYAPLANRLGIWQLKWELEDLAFRFEDPITYKRIAKLLDEKRAEREAYVAQAIERLQQELAAANVQA
DVSGRPKHIYSIWRKMRGKELDFSELYDVRAFRVIVPDIKDCYAVLGIVHHLWQPVPKEFDDYISRPKPNGYKSLHTVVI
GDDGRAFEVQIRTQEMHRFAEYGVAAHWRYKEAGTRGYGGQFSASDKYDEKIAWLRQLLAWKDDVEDGAEQSGDKAWAQL
RETSLDDDHIYVLTPQARVIALPQGATPVDFAYHLHSELGHRCRGARVDGAMVPLNTPLANGQTVEIVAVKEGGPSRDWL
NPQLGYLKSPRARQKVRAWFNAIEQEENIAHGRALVEKTLQREGKTSVSLDSLAAKLGFKSPEELFSVVGKEEFSLRNIE
QALSDAPPAEPAPEAPADFEKRSSGASVAHGASTGVLVVGVDALLTQLARCCRPAPPDPISGFVTRGKGMSIHRSDCATF
RRMAERAPERVLHTTWSADVLGGRGASVYPVDLMIEATDRQGLLRDISEVFAREKMNVVGVKTQSRRNTAFMQFTVEVSN
SAQVQRACTLLGEVQGVVRAGRKA
>Mature_743_residues
TESVSASSVAAPSFDDVLAFVRERAGDACLASGERLADHAAGTAAIMRTLNVDPPAMQAAALFALTPHLSDPERELTERF
GDEVARLVADVRKLLRLGTVSLRAAQHAAPEAGRDAAQQRRAQIEALRKMLLAFAQDIRVVLIRLASRLQSLRYYAAAKL
EPPPEVARETLEIYAPLANRLGIWQLKWELEDLAFRFEDPITYKRIAKLLDEKRAEREAYVAQAIERLQQELAAANVQAD
VSGRPKHIYSIWRKMRGKELDFSELYDVRAFRVIVPDIKDCYAVLGIVHHLWQPVPKEFDDYISRPKPNGYKSLHTVVIG
DDGRAFEVQIRTQEMHRFAEYGVAAHWRYKEAGTRGYGGQFSASDKYDEKIAWLRQLLAWKDDVEDGAEQSGDKAWAQLR
ETSLDDDHIYVLTPQARVIALPQGATPVDFAYHLHSELGHRCRGARVDGAMVPLNTPLANGQTVEIVAVKEGGPSRDWLN
PQLGYLKSPRARQKVRAWFNAIEQEENIAHGRALVEKTLQREGKTSVSLDSLAAKLGFKSPEELFSVVGKEEFSLRNIEQ
ALSDAPPAEPAPEAPADFEKRSSGASVAHGASTGVLVVGVDALLTQLARCCRPAPPDPISGFVTRGKGMSIHRSDCATFR
RMAERAPERVLHTTWSADVLGGRGASVYPVDLMIEATDRQGLLRDISEVFAREKMNVVGVKTQSRRNTAFMQFTVEVSNS
AQVQRACTLLGEVQGVVRAGRKA

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Escherichia coli, GI1789147, Length=706, Percent_Identity=40.7932011331445, Blast_Score=536, Evalue=1e-153,
Organism=Escherichia coli, GI1790082, Length=728, Percent_Identity=33.6538461538462, Blast_Score=387, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 82462; Mature: 82330

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESVSASSVAAPSFDDVLAFVRERAGDACLASGERLADHAAGTAAIMRTLNVDPPAMQA
CCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHH
AALFALTPHLSDPERELTERFGDEVARLVADVRKLLRLGTVSLRAAQHAAPEAGRDAAQQ
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
RRAQIEALRKMLLAFAQDIRVVLIRLASRLQSLRYYAAAKLEPPPEVARETLEIYAPLAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RLGIWQLKWELEDLAFRFEDPITYKRIAKLLDEKRAEREAYVAQAIERLQQELAAANVQA
CCCEEEEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DVSGRPKHIYSIWRKMRGKELDFSELYDVRAFRVIVPDIKDCYAVLGIVHHLWQPVPKEF
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHCCCCHHH
DDYISRPKPNGYKSLHTVVIGDDGRAFEVQIRTQEMHRFAEYGVAAHWRYKEAGTRGYGG
HHHHCCCCCCCCCCEEEEEECCCCCEEEEEEEHHHHHHHHHCCCHHEEEEHHCCCCCCCC
QFSASDKYDEKIAWLRQLLAWKDDVEDGAEQSGDKAWAQLRETSLDDDHIYVLTPQARVI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCEEE
ALPQGATPVDFAYHLHSELGHRCRGARVDGAMVPLNTPLANGQTVEIVAVKEGGPSRDWL
EECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEEEECCCCCCCCC
NPQLGYLKSPRARQKVRAWFNAIEQEENIAHGRALVEKTLQREGKTSVSLDSLAAKLGFK
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCC
SPEELFSVVGKEEFSLRNIEQALSDAPPAEPAPEAPADFEKRSSGASVAHGASTGVLVVG
CHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCEECCCCCCEEEEE
VDALLTQLARCCRPAPPDPISGFVTRGKGMSIHRSDCATFRRMAERAPERVLHTTWSADV
HHHHHHHHHHHCCCCCCCCCCHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCC
LGGRGASVYPVDLMIEATDRQGLLRDISEVFAREKMNVVGVKTQSRRNTAFMQFTVEVSN
CCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEEEEECC
SAQVQRACTLLGEVQGVVRAGRKA
CHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TESVSASSVAAPSFDDVLAFVRERAGDACLASGERLADHAAGTAAIMRTLNVDPPAMQA
CCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHH
AALFALTPHLSDPERELTERFGDEVARLVADVRKLLRLGTVSLRAAQHAAPEAGRDAAQQ
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
RRAQIEALRKMLLAFAQDIRVVLIRLASRLQSLRYYAAAKLEPPPEVARETLEIYAPLAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RLGIWQLKWELEDLAFRFEDPITYKRIAKLLDEKRAEREAYVAQAIERLQQELAAANVQA
CCCEEEEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DVSGRPKHIYSIWRKMRGKELDFSELYDVRAFRVIVPDIKDCYAVLGIVHHLWQPVPKEF
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHCCCCHHH
DDYISRPKPNGYKSLHTVVIGDDGRAFEVQIRTQEMHRFAEYGVAAHWRYKEAGTRGYGG
HHHHCCCCCCCCCCEEEEEECCCCCEEEEEEEHHHHHHHHHCCCHHEEEEHHCCCCCCCC
QFSASDKYDEKIAWLRQLLAWKDDVEDGAEQSGDKAWAQLRETSLDDDHIYVLTPQARVI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCEEE
ALPQGATPVDFAYHLHSELGHRCRGARVDGAMVPLNTPLANGQTVEIVAVKEGGPSRDWL
EECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEEEECCCCCCCCC
NPQLGYLKSPRARQKVRAWFNAIEQEENIAHGRALVEKTLQREGKTSVSLDSLAAKLGFK
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCC
SPEELFSVVGKEEFSLRNIEQALSDAPPAEPAPEAPADFEKRSSGASVAHGASTGVLVVG
CHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCEECCCCCCEEEEE
VDALLTQLARCCRPAPPDPISGFVTRGKGMSIHRSDCATFRRMAERAPERVLHTTWSADV
HHHHHHHHHHHCCCCCCCCCCHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCC
LGGRGASVYPVDLMIEATDRQGLLRDISEVFAREKMNVVGVKTQSRRNTAFMQFTVEVSN
CCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEEEEECC
SAQVQRACTLLGEVQGVVRAGRKA
CHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7928955 [H]