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The map label for this gene is truB [H]

Identifier: 161524926

GI number: 161524926

Start: 1940163

End: 1941095

Strand: Reverse

Name: truB [H]

Synonym: Bmul_1753

Alternate gene names: 161524926

Gene position: 1941095-1940163 (Counterclockwise)

Preceding gene: 161524927

Following gene: 161524921

Centisome position: 56.29

GC content: 69.02

Gene sequence:

>933_bases
ATGACGACTGCAGCATCCCCACGTCCGCGCGTACCGCGCCGCGCGCTGGACGGCGTCCTGCTGCTCGACAAGCCGGTCGG
CCTGTCGAGCAACGATGCGCTGATTCGCGCGAAGCGCCTGTACCTCGCGAAGAAGGCCGGGCATACCGGCACGCTCGACC
CGCTCGCATCGGGCCTGCTGCCGCTGTGCTTCGGCGAAGCGACGAAGTTCTCGCAGGATCTGCTCGAGGCGGACAAGACC
TATGAAGCGACGATGCGGCTCGGCGTGCGTACCGCGACCGGCGACGCCGAAGGCGAGGTGCTTGACACGCGGCCCGTCGC
ATGCGATCGCGCGGCCGTCGAAGCGGCGCTCGTGCGTTTCACGGGCGACATCGTGCAGGTGCCGCCGATGTATTCGGCGC
TCAAGCGCGACGGCAAGCCGCTGTACGAATATGCGCGTGCCGGTCAGACGGTCGAGCGCGAAGGTCGCAGCGTGACGATC
CACGCGCTCGACCTGCTCGCATGCGAACTGCCCGACGTGACGTTTCGCGTCACGTGCAGCAAGGGCACGTATGTGCGCAC
GCTCGCGGAAGACATCGGCGAAGCGCTCGGCTGTGGCGCGCATCTGACGATGCTGCGCCGCACGGGCGTCGGCGCGCTGA
CGCTCGAACATGCGGTGACGCTCGATGCGTTGTCCGATGCGGACGAAGCGGCGCGCGACGCCTGGCTTCAGCCCGTCGAT
GCGCTGCTGTCGACGTTCCCGCTCGTGCGTCTCGACGACACCTGCGCGAAGCGCTTCCTGCACGGGCAGCGTTTGCCGCT
GTCGGCGCTGGAGGCGATCGACGCCGCCGAAGGCGAACGCGTGCGCGTGTACGACGCGACGCGGCTGCTCGGCGTCGCGC
GCAAGACGAACGGCGTGCTCGCGCCGGAACGGCTCGTCGTCACCGCGCAATGA

Upstream 100 bases:

>100_bases
GTCGCGATGTCGCGTCTGATCGACGAGGCGAACGCGACGCGGGCGAAGGACGACGACGAGGCCGACGCGCCGGCGAAGGA
CGACTGAGCGTATCCCGCCT

Downstream 100 bases:

>100_bases
CGCGCGCACGCACGTGCGCCGTCGGAACGAAAAAAGCCCGGTCGAGACCGGGCTTTTCGTTTGCTGCGCGGATGCGCGCG
ACGTCTAATGCGCGGCCGAT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MTTAASPRPRVPRRALDGVLLLDKPVGLSSNDALIRAKRLYLAKKAGHTGTLDPLASGLLPLCFGEATKFSQDLLEADKT
YEATMRLGVRTATGDAEGEVLDTRPVACDRAAVEAALVRFTGDIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRSVTI
HALDLLACELPDVTFRVTCSKGTYVRTLAEDIGEALGCGAHLTMLRRTGVGALTLEHAVTLDALSDADEAARDAWLQPVD
ALLSTFPLVRLDDTCAKRFLHGQRLPLSALEAIDAAEGERVRVYDATRLLGVARKTNGVLAPERLVVTAQ

Sequences:

>Translated_310_residues
MTTAASPRPRVPRRALDGVLLLDKPVGLSSNDALIRAKRLYLAKKAGHTGTLDPLASGLLPLCFGEATKFSQDLLEADKT
YEATMRLGVRTATGDAEGEVLDTRPVACDRAAVEAALVRFTGDIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRSVTI
HALDLLACELPDVTFRVTCSKGTYVRTLAEDIGEALGCGAHLTMLRRTGVGALTLEHAVTLDALSDADEAARDAWLQPVD
ALLSTFPLVRLDDTCAKRFLHGQRLPLSALEAIDAAEGERVRVYDATRLLGVARKTNGVLAPERLVVTAQ
>Mature_309_residues
TTAASPRPRVPRRALDGVLLLDKPVGLSSNDALIRAKRLYLAKKAGHTGTLDPLASGLLPLCFGEATKFSQDLLEADKTY
EATMRLGVRTATGDAEGEVLDTRPVACDRAAVEAALVRFTGDIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRSVTIH
ALDLLACELPDVTFRVTCSKGTYVRTLAEDIGEALGCGAHLTMLRRTGVGALTLEHAVTLDALSDADEAARDAWLQPVDA
LLSTFPLVRLDDTCAKRFLHGQRLPLSALEAIDAAEGERVRVYDATRLLGVARKTNGVLAPERLVVTAQ

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=252, Percent_Identity=36.1111111111111, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI4503337, Length=261, Percent_Identity=27.5862068965517, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI215599015, Length=261, Percent_Identity=27.5862068965517, Blast_Score=78, Evalue=9e-15,
Organism=Escherichia coli, GI2367200, Length=316, Percent_Identity=46.2025316455696, Blast_Score=259, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17553978, Length=257, Percent_Identity=29.5719844357977, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324037, Length=199, Percent_Identity=34.1708542713568, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323204, Length=284, Percent_Identity=28.5211267605634, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI281364189, Length=262, Percent_Identity=27.4809160305344, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281364187, Length=262, Percent_Identity=27.4809160305344, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281364185, Length=262, Percent_Identity=27.4809160305344, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281364183, Length=262, Percent_Identity=27.4809160305344, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI62471759, Length=262, Percent_Identity=27.4809160305344, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17975520, Length=262, Percent_Identity=27.4809160305344, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 33359; Mature: 33227

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAASPRPRVPRRALDGVLLLDKPVGLSSNDALIRAKRLYLAKKAGHTGTLDPLASGLL
CCCCCCCCCCCCHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCH
PLCFGEATKFSQDLLEADKTYEATMRLGVRTATGDAEGEVLDTRPVACDRAAVEAALVRF
HHHHCCHHHHHHHHHHHCHHHHHHHHHCEEEECCCCCCCEEECCCCCHHHHHHHHHHHHH
TGDIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRSVTIHALDLLACELPDVTFRVTCS
CCCEEECCCHHHHHHHCCCHHHHHHHCCCCHHHCCCEEEEEEEEEHHHCCCCCEEEEEEC
KGTYVRTLAEDIGEALGCGAHLTMLRRTGVGALTLEHAVTLDALSDADEAARDAWLQPVD
CCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEHHHHHHHHHHCCHHHHHHHHHHHHHH
ALLSTFPLVRLDDTCAKRFLHGQRLPLSALEAIDAAEGERVRVYDATRLLGVARKTNGVL
HHHHHCCCEEECHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEEHHHHHHHHHHCCCCC
APERLVVTAQ
CCCEEEEEEC
>Mature Secondary Structure 
TTAASPRPRVPRRALDGVLLLDKPVGLSSNDALIRAKRLYLAKKAGHTGTLDPLASGLL
CCCCCCCCCCCHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCH
PLCFGEATKFSQDLLEADKTYEATMRLGVRTATGDAEGEVLDTRPVACDRAAVEAALVRF
HHHHCCHHHHHHHHHHHCHHHHHHHHHCEEEECCCCCCCEEECCCCCHHHHHHHHHHHHH
TGDIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRSVTIHALDLLACELPDVTFRVTCS
CCCEEECCCHHHHHHHCCCHHHHHHHCCCCHHHCCCEEEEEEEEEHHHCCCCCEEEEEEC
KGTYVRTLAEDIGEALGCGAHLTMLRRTGVGALTLEHAVTLDALSDADEAARDAWLQPVD
CCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEHHHHHHHHHHCCHHHHHHHHHHHHHH
ALLSTFPLVRLDDTCAKRFLHGQRLPLSALEAIDAAEGERVRVYDATRLLGVARKTNGVL
HHHHHCCCEEECHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEEHHHHHHHHHHCCCCC
APERLVVTAQ
CCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA