Click here to switch to the map view.

The map label for this gene is cysU [H]

Identifier: 161524808

GI number: 161524808

Start: 1811960

End: 1812859

Strand: Reverse

Name: cysU [H]

Synonym: Bmul_1635

Alternate gene names: 161524808

Gene position: 1812859-1811960 (Counterclockwise)

Preceding gene: 161524809

Following gene: 161524807

Centisome position: 52.57

GC content: 68.44

Gene sequence:

>900_bases
ATGACCACGTACACCTTTCGCAAGCCGAGCGCGCTGCCCGGTTTCGGCGTGACGCTCGGCATCACGGTGGCCTATTTGAG
CCTCGTGGTGCTGATTCCGCTCGCCGCCACGTTCCTGAAGACCGCGACGCTGTCGTGGGACCAGTTCGTCGCCGCCGTCA
CGTCGCCGCGCGTGCTCGCGTCGTACCGGCTGACGTTCTCGGCCGCATTCGGCGGTGCGCTGATCAACGCCGTGTTCGGC
TTTCTCGTCGCGTGGGTGCTCGTGCGCTACACGTTTCCGCTGAAGCGGCTCGTCGACGCGATCGTCGATCTGCCGTTCGC
GCTGCCGACCTCGGTGGCCGGCATTTCGCTCGCGGCCGTCTACGCGGCGAACGGCTGGGTCGGCCAGTATCTCGCGCCGT
TCGGCATCAAGGTCGCGTTCACGCCGCTCGGCGTGCTGGTCGCGCTGACCTTCATCGGCCTGCCGTTCGTCGTGCGCACC
GTGCAGCCGGTGCTCGAGGATTTCGAGCGCGAGCAGGAGGAGGCGGCCGCGTGCCTCGGCGCGTCGCGCTGGCTCACGTT
CCGGCGCGTCGTGCTGCCGGCCGTGCTGCCCGCGCTGCTCACCGGTTTCGCGCTCGCGTTCGCGCGTGCGCTCGGCGAAT
ACGGGTCGGTGATCTTCATCGCCGGCAACGTGCCGATGAAGTCGGAGATCACGTCGCTGCTGATCATCACGAAGCTCGAA
CAGTACGACTACGCGGGCGCGACCGCGCTCGCGGTCGTGATGCTCGTCGTGTCGTTCCTGATGCTGCTGCTGATCAATAC
GCTGCAGTGGTATCTGCAGCGCCGTACGAGCCGCGGCGCGAGCGCGCCCGCGTCCGCGTCCGGTGATACCGCCGCGGTCG
CGGCAGGAGGCCGGCCATGA

Upstream 100 bases:

>100_bases
GCGCGCCACGTAGTGCGCCGCGTCCCGGACGCCGCCGGCGCGACCTGTCGCGCCGGCGTTCGCGTCGGGCCCATGAGCAT
CGACCCAGCAAGAGCATCCG

Downstream 100 bases:

>100_bases
CGCAGGAGGTGACCGCCGTGCTCAAGAACCCGTCATCCGCCGAGCGTGCGCGCGCGGCGAAGCGGCTCGACCCCGTCAGC
GAGCCGCGCGCCGTGCGCTG

Product: sulfate ABC transporter inner membrane subunit CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MTTYTFRKPSALPGFGVTLGITVAYLSLVVLIPLAATFLKTATLSWDQFVAAVTSPRVLASYRLTFSAAFGGALINAVFG
FLVAWVLVRYTFPLKRLVDAIVDLPFALPTSVAGISLAAVYAANGWVGQYLAPFGIKVAFTPLGVLVALTFIGLPFVVRT
VQPVLEDFEREQEEAAACLGASRWLTFRRVVLPAVLPALLTGFALAFARALGEYGSVIFIAGNVPMKSEITSLLIITKLE
QYDYAGATALAVVMLVVSFLMLLLINTLQWYLQRRTSRGASAPASASGDTAAVAAGGRP

Sequences:

>Translated_299_residues
MTTYTFRKPSALPGFGVTLGITVAYLSLVVLIPLAATFLKTATLSWDQFVAAVTSPRVLASYRLTFSAAFGGALINAVFG
FLVAWVLVRYTFPLKRLVDAIVDLPFALPTSVAGISLAAVYAANGWVGQYLAPFGIKVAFTPLGVLVALTFIGLPFVVRT
VQPVLEDFEREQEEAAACLGASRWLTFRRVVLPAVLPALLTGFALAFARALGEYGSVIFIAGNVPMKSEITSLLIITKLE
QYDYAGATALAVVMLVVSFLMLLLINTLQWYLQRRTSRGASAPASASGDTAAVAAGGRP
>Mature_298_residues
TTYTFRKPSALPGFGVTLGITVAYLSLVVLIPLAATFLKTATLSWDQFVAAVTSPRVLASYRLTFSAAFGGALINAVFGF
LVAWVLVRYTFPLKRLVDAIVDLPFALPTSVAGISLAAVYAANGWVGQYLAPFGIKVAFTPLGVLVALTFIGLPFVVRTV
QPVLEDFEREQEEAAACLGASRWLTFRRVVLPAVLPALLTGFALAFARALGEYGSVIFIAGNVPMKSEITSLLIITKLEQ
YDYAGATALAVVMLVVSFLMLLLINTLQWYLQRRTSRGASAPASASGDTAAVAAGGRP

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=264, Percent_Identity=51.5151515151515, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI87082099, Length=271, Percent_Identity=32.4723247232472, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1786980, Length=202, Percent_Identity=34.6534653465347, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786254, Length=227, Percent_Identity=28.6343612334802, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787369, Length=172, Percent_Identity=26.7441860465116, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787368, Length=224, Percent_Identity=29.9107142857143, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31834; Mature: 31703

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTYTFRKPSALPGFGVTLGITVAYLSLVVLIPLAATFLKTATLSWDQFVAAVTSPRVLA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHH
SYRLTFSAAFGGALINAVFGFLVAWVLVRYTFPLKRLVDAIVDLPFALPTSVAGISLAAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
YAANGWVGQYLAPFGIKVAFTPLGVLVALTFIGLPFVVRTVQPVLEDFEREQEEAAACLG
HHCCCCHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASRWLTFRRVVLPAVLPALLTGFALAFARALGEYGSVIFIAGNVPMKSEITSLLIITKLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHH
QYDYAGATALAVVMLVVSFLMLLLINTLQWYLQRRTSRGASAPASASGDTAAVAAGGRP
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCC
>Mature Secondary Structure 
TTYTFRKPSALPGFGVTLGITVAYLSLVVLIPLAATFLKTATLSWDQFVAAVTSPRVLA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHH
SYRLTFSAAFGGALINAVFGFLVAWVLVRYTFPLKRLVDAIVDLPFALPTSVAGISLAAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
YAANGWVGQYLAPFGIKVAFTPLGVLVALTFIGLPFVVRTVQPVLEDFEREQEEAAACLG
HHCCCCHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASRWLTFRRVVLPAVLPALLTGFALAFARALGEYGSVIFIAGNVPMKSEITSLLIITKLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHH
QYDYAGATALAVVMLVVSFLMLLLINTLQWYLQRRTSRGASAPASASGDTAAVAAGGRP
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]