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The map label for this gene is cysW [H]

Identifier: 161524807

GI number: 161524807

Start: 1811016

End: 1811963

Strand: Reverse

Name: cysW [H]

Synonym: Bmul_1634

Alternate gene names: 161524807

Gene position: 1811963-1811016 (Counterclockwise)

Preceding gene: 161524808

Following gene: 161524806

Centisome position: 52.54

GC content: 68.14

Gene sequence:

>948_bases
ATGACGCAGGAGGTGACCGCCGTGCTCAAGAACCCGTCATCCGCCGAGCGTGCGCGCGCGGCGAAGCGGCTCGACCCCGT
CAGCGAGCCGCGCGCCGTGCGCTGGCTGCTCACCGGCATCGCGCTCGCGTTTCTGGCCTTCTTTCTCGTCGTGCCGCTCG
CGGCCGTGTTCGTCGAAGCGCTGCGCAAGGGCGTCGGCTTCTATCTCGAGTCGCTCGCGGATCCGGACGCGTGGGCCGCG
ATCAAGCTGACGCTGACCGTCGCGGCGATCGCCGTGCCGCTGAACCTCGTGTTCGGCGTGTGCGCGTCGTGGGCGATCGC
GAAATTCGAGTTTCGCGGCAAGGCGCTGCTGACGACGCTGATCGATCTGCCGTTCTCGGTCTCGCCGGTGATCTCGGGCC
TCGTCTACGTGCTGCTGTTCGGCGCGCAGGGCTGGCTCGGCCCGTGGCTGCAGGATCACGACGTGCAGATCATCTTCGCC
GTGCCGGGCATCGTGCTCGCGACGATCTTCGTCACGTTCCCGTTCGTCGCGCGCGAGCTGATTCCGCTGATGCAGGCACA
GGGCAGCGACGAGGAAGAGGCCGCGCGCGTGCTCGGCGCGTCGGGCTGGCAGATCTTCCGCCGCGTGACGCTGCCGAACG
TGAAGTGGGGGCTGCTGTACGGCGTGATCCTCTGCAATGCGCGTGCGATGGGCGAATTCGGCGCGGTGTCGGTCGTGTCC
GGCCATATCCGCGGCCAGACCGACACGATGCCGCTGCACGTCGAGATTCTCTACAACGAGTACAACTTCGCCGCGGCGTT
CGCGGTGGCATCGGTGCTCGCGCTGCTCGCGCTCGTGACGCTCGCGCTGAAGCTGCTCGCCGAACGCCATCTCGCCGCGG
AGCTGTCCGGCACGCGCGACGCCGTTCCCGCACATGCCGGCCCTGCCGCCATTTCGTCGAAATCGTAA

Upstream 100 bases:

>100_bases
ATACGCTGCAGTGGTATCTGCAGCGCCGTACGAGCCGCGGCGCGAGCGCGCCCGCGTCCGCGTCCGGTGATACCGCCGCG
GTCGCGGCAGGAGGCCGGCC

Downstream 100 bases:

>100_bases
AGAGGCAACCAGAATGGGTATCACCGTCCGTCATCTTCAGAAGCGCTTCGGCGATTTCACCGCGCTCGACAACGTGTCGC
TCGATTTCCCGCCCGGCGAG

Product: sulfate ABC transporter inner membrane subunit CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MTQEVTAVLKNPSSAERARAAKRLDPVSEPRAVRWLLTGIALAFLAFFLVVPLAAVFVEALRKGVGFYLESLADPDAWAA
IKLTLTVAAIAVPLNLVFGVCASWAIAKFEFRGKALLTTLIDLPFSVSPVISGLVYVLLFGAQGWLGPWLQDHDVQIIFA
VPGIVLATIFVTFPFVARELIPLMQAQGSDEEEAARVLGASGWQIFRRVTLPNVKWGLLYGVILCNARAMGEFGAVSVVS
GHIRGQTDTMPLHVEILYNEYNFAAAFAVASVLALLALVTLALKLLAERHLAAELSGTRDAVPAHAGPAAISSKS

Sequences:

>Translated_315_residues
MTQEVTAVLKNPSSAERARAAKRLDPVSEPRAVRWLLTGIALAFLAFFLVVPLAAVFVEALRKGVGFYLESLADPDAWAA
IKLTLTVAAIAVPLNLVFGVCASWAIAKFEFRGKALLTTLIDLPFSVSPVISGLVYVLLFGAQGWLGPWLQDHDVQIIFA
VPGIVLATIFVTFPFVARELIPLMQAQGSDEEEAARVLGASGWQIFRRVTLPNVKWGLLYGVILCNARAMGEFGAVSVVS
GHIRGQTDTMPLHVEILYNEYNFAAAFAVASVLALLALVTLALKLLAERHLAAELSGTRDAVPAHAGPAAISSKS
>Mature_314_residues
TQEVTAVLKNPSSAERARAAKRLDPVSEPRAVRWLLTGIALAFLAFFLVVPLAAVFVEALRKGVGFYLESLADPDAWAAI
KLTLTVAAIAVPLNLVFGVCASWAIAKFEFRGKALLTTLIDLPFSVSPVISGLVYVLLFGAQGWLGPWLQDHDVQIIFAV
PGIVLATIFVTFPFVARELIPLMQAQGSDEEEAARVLGASGWQIFRRVTLPNVKWGLLYGVILCNARAMGEFGAVSVVSG
HIRGQTDTMPLHVEILYNEYNFAAAFAVASVLALLALVTLALKLLAERHLAAELSGTRDAVPAHAGPAAISSKS

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=261, Percent_Identity=55.5555555555556, Blast_Score=304, Evalue=4e-84,
Organism=Escherichia coli, GI1788764, Length=206, Percent_Identity=37.8640776699029, Blast_Score=150, Evalue=8e-38,
Organism=Escherichia coli, GI1786980, Length=181, Percent_Identity=35.9116022099448, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787369, Length=175, Percent_Identity=27.4285714285714, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33744; Mature: 33613

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQEVTAVLKNPSSAERARAAKRLDPVSEPRAVRWLLTGIALAFLAFFLVVPLAAVFVEA
CCCHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRKGVGFYLESLADPDAWAAIKLTLTVAAIAVPLNLVFGVCASWAIAKFEFRGKALLTTL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
IDLPFSVSPVISGLVYVLLFGAQGWLGPWLQDHDVQIIFAVPGIVLATIFVTFPFVAREL
HHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHH
IPLMQAQGSDEEEAARVLGASGWQIFRRVTLPNVKWGLLYGVILCNARAMGEFGAVSVVS
HHHHHCCCCCHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHCCHHCCCCCCHHHHC
GHIRGQTDTMPLHVEILYNEYNFAAAFAVASVLALLALVTLALKLLAERHLAAELSGTRD
CCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AVPAHAGPAAISSKS
CCCCCCCCCCCCCCC
>Mature Secondary Structure 
TQEVTAVLKNPSSAERARAAKRLDPVSEPRAVRWLLTGIALAFLAFFLVVPLAAVFVEA
CCHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRKGVGFYLESLADPDAWAAIKLTLTVAAIAVPLNLVFGVCASWAIAKFEFRGKALLTTL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
IDLPFSVSPVISGLVYVLLFGAQGWLGPWLQDHDVQIIFAVPGIVLATIFVTFPFVAREL
HHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHH
IPLMQAQGSDEEEAARVLGASGWQIFRRVTLPNVKWGLLYGVILCNARAMGEFGAVSVVS
HHHHHCCCCCHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHCCHHCCCCCCHHHHC
GHIRGQTDTMPLHVEILYNEYNFAAAFAVASVLALLALVTLALKLLAERHLAAELSGTRD
CCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AVPAHAGPAAISSKS
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]