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The map label for this gene is purR [H]

Identifier: 161524801

GI number: 161524801

Start: 1803844

End: 1804875

Strand: Reverse

Name: purR [H]

Synonym: Bmul_1628

Alternate gene names: 161524801

Gene position: 1804875-1803844 (Counterclockwise)

Preceding gene: 161524802

Following gene: 161524800

Centisome position: 52.34

GC content: 69.38

Gene sequence:

>1032_bases
ATGGCGACGATCAAGGATGTGGCGGCCATGGCGGGCGTGTCGTTTACGACGGTATCGCACGTGGTGAACAACTCGCGGCC
GGTGTCCGCGGACGTACGGGCGAAGGTCGAGGGCGCGATCCGCGAGCTGAACTACGTGCCGTCGGCGGTCGCGCGCTCGC
TGAAGGCCCGCGCGACGGCGACGATCGGCCTCGTCGTGCCGAACAGCACGAATCCGTACTTCGCGGAGCTCGCGCGCGGC
ATCGAGGATCAGTGCGCGGCCAACGGCTACTGCGTGTTTTTCTGCAACTCGGACGACGATCCGGTCAAGCAGCGCAACTA
CCTGCGCGTGCTGCAGGAAAAGCGCATCGACGGGCTGATCGTCGCGTCGGCCGGCGAAGATGCCGTGCTCGCGCAGACGC
TCGCCGACGCGCATGCGCCGCTCGTCGTCGTCGACCGCAACATCGAAGGGCTCGCGGCCGATCTCGTGCAGATCGATCAC
GAGCGCGGCGCCTATCTCGCGACGCGCCATCTGCTCGAACTCGGCCACGCGAAGATCGGCTGCATCACCGGGCCGACCGA
CACGGCCGTCAGCGCGATGCGCGTGCACGGCTTCATCCGCGCGATGGCCGAGCGCGGCATCGACATCGTGCCGGGCGCGA
TCGCGGAAAGCGACTTTTCCTGCCTCGGCGGCTATCACGCGGCGGCGCGGCTGTTCGAATCGGTGCGGCCGAGCGCGATC
TTCGCGGGCAACGACCTGATGGGCGTCGGCGCGCTGCGCGCGGCCGCCGAGCGCGGCATGCGCGTGCCCGACGACTGTTC
GATCATCGGTTTCGACGACATCGAATTCTCGCGTTACACGTATCCGGCGCTGTCGACCGTCGGCCAGTCGGTGCGCGCGC
TCGGCGAAATGGCCGCGCAGACGCTGATCGAGCGGATCGGCGGTGGTTCGTCGGCGGCGCCGAGCCGGCGGCGCGTCGTG
TCGCCGCGGCTCGTGCTGCGCGAATCGACGGCGATCTATCGCGAGCCGGCCGCGTCCGGCTCCCGCGCATGA

Upstream 100 bases:

>100_bases
GGGGCGGTGATCGTGGTCGCGGTCGTGCTCGACACGTATCGCAGCCGGCGCACGCGCACGCGGTAAGACCAACAACAGAA
AGAGCAGAGAGAAACGGGAA

Downstream 100 bases:

>100_bases
CGGCCGGCGCGGCAGCGCGTGCCGGCCGCGTCGCGGTGGTCGGCAGCCTCAACATGGATCTCGTCGTGCGCGCGCCGCGG
CTGCCGCTGCCGGGCGAGAC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MATIKDVAAMAGVSFTTVSHVVNNSRPVSADVRAKVEGAIRELNYVPSAVARSLKARATATIGLVVPNSTNPYFAELARG
IEDQCAANGYCVFFCNSDDDPVKQRNYLRVLQEKRIDGLIVASAGEDAVLAQTLADAHAPLVVVDRNIEGLAADLVQIDH
ERGAYLATRHLLELGHAKIGCITGPTDTAVSAMRVHGFIRAMAERGIDIVPGAIAESDFSCLGGYHAAARLFESVRPSAI
FAGNDLMGVGALRAAAERGMRVPDDCSIIGFDDIEFSRYTYPALSTVGQSVRALGEMAAQTLIERIGGGSSAAPSRRRVV
SPRLVLRESTAIYREPAASGSRA

Sequences:

>Translated_343_residues
MATIKDVAAMAGVSFTTVSHVVNNSRPVSADVRAKVEGAIRELNYVPSAVARSLKARATATIGLVVPNSTNPYFAELARG
IEDQCAANGYCVFFCNSDDDPVKQRNYLRVLQEKRIDGLIVASAGEDAVLAQTLADAHAPLVVVDRNIEGLAADLVQIDH
ERGAYLATRHLLELGHAKIGCITGPTDTAVSAMRVHGFIRAMAERGIDIVPGAIAESDFSCLGGYHAAARLFESVRPSAI
FAGNDLMGVGALRAAAERGMRVPDDCSIIGFDDIEFSRYTYPALSTVGQSVRALGEMAAQTLIERIGGGSSAAPSRRRVV
SPRLVLRESTAIYREPAASGSRA
>Mature_342_residues
ATIKDVAAMAGVSFTTVSHVVNNSRPVSADVRAKVEGAIRELNYVPSAVARSLKARATATIGLVVPNSTNPYFAELARGI
EDQCAANGYCVFFCNSDDDPVKQRNYLRVLQEKRIDGLIVASAGEDAVLAQTLADAHAPLVVVDRNIEGLAADLVQIDHE
RGAYLATRHLLELGHAKIGCITGPTDTAVSAMRVHGFIRAMAERGIDIVPGAIAESDFSCLGGYHAAARLFESVRPSAIF
AGNDLMGVGALRAAAERGMRVPDDCSIIGFDDIEFSRYTYPALSTVGQSVRALGEMAAQTLIERIGGGSSAAPSRRRVVS
PRLVLRESTAIYREPAASGSRA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=331, Percent_Identity=40.4833836858006, Blast_Score=247, Evalue=8e-67,
Organism=Escherichia coli, GI1787948, Length=334, Percent_Identity=42.5149700598802, Blast_Score=245, Evalue=3e-66,
Organism=Escherichia coli, GI1790369, Length=332, Percent_Identity=33.1325301204819, Blast_Score=191, Evalue=4e-50,
Organism=Escherichia coli, GI1788474, Length=342, Percent_Identity=36.5497076023392, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1789068, Length=333, Percent_Identity=31.8318318318318, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1789202, Length=334, Percent_Identity=34.1317365269461, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1786540, Length=305, Percent_Identity=31.4754098360656, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1787580, Length=317, Percent_Identity=29.9684542586751, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI48994940, Length=312, Percent_Identity=27.5641025641026, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1790715, Length=316, Percent_Identity=25.6329113924051, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1787906, Length=342, Percent_Identity=26.6081871345029, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786268, Length=328, Percent_Identity=26.5243902439024, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789456, Length=322, Percent_Identity=27.639751552795, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790689, Length=316, Percent_Identity=24.3670886075949, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790192, Length=248, Percent_Identity=28.2258064516129, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36370; Mature: 36239

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVAAMAGVSFTTVSHVVNNSRPVSADVRAKVEGAIRELNYVPSAVARSLKARATA
CCCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCEE
TIGLVVPNSTNPYFAELARGIEDQCAANGYCVFFCNSDDDPVKQRNYLRVLQEKRIDGLI
EEEEEECCCCCHHHHHHHCCHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEE
VASAGEDAVLAQTLADAHAPLVVVDRNIEGLAADLVQIDHERGAYLATRHLLELGHAKIG
EECCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEE
CITGPTDTAVSAMRVHGFIRAMAERGIDIVPGAIAESDFSCLGGYHAAARLFESVRPSAI
EECCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
FAGNDLMGVGALRAAAERGMRVPDDCSIIGFDDIEFSRYTYPALSTVGQSVRALGEMAAQ
EECCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TLIERIGGGSSAAPSRRRVVSPRLVLRESTAIYREPAASGSRA
HHHHHHCCCCCCCCCHHHHCCCHHHEECCHHHHCCCCCCCCCC
>Mature Secondary Structure 
ATIKDVAAMAGVSFTTVSHVVNNSRPVSADVRAKVEGAIRELNYVPSAVARSLKARATA
CCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCEE
TIGLVVPNSTNPYFAELARGIEDQCAANGYCVFFCNSDDDPVKQRNYLRVLQEKRIDGLI
EEEEEECCCCCHHHHHHHCCHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEE
VASAGEDAVLAQTLADAHAPLVVVDRNIEGLAADLVQIDHERGAYLATRHLLELGHAKIG
EECCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEE
CITGPTDTAVSAMRVHGFIRAMAERGIDIVPGAIAESDFSCLGGYHAAARLFESVRPSAI
EECCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
FAGNDLMGVGALRAAAERGMRVPDDCSIIGFDDIEFSRYTYPALSTVGQSVRALGEMAAQ
EECCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TLIERIGGGSSAAPSRRRVVSPRLVLRESTAIYREPAASGSRA
HHHHHHCCCCCCCCCHHHHCCCHHHEECCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA