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The map label for this gene is sdhA [C]

Identifier: 161524471

GI number: 161524471

Start: 1426305

End: 1428044

Strand: Reverse

Name: sdhA [C]

Synonym: Bmul_1298

Alternate gene names: 161524471

Gene position: 1428044-1426305 (Counterclockwise)

Preceding gene: 161524472

Following gene: 161524470

Centisome position: 41.41

GC content: 66.32

Gene sequence:

>1740_bases
ATGAACACCCATGTACTCGAATACGACATCGTCGTGGTCGGCGGCGGCACGGCCGGCCCGATGGCCGCGATCAAGGCGAA
GGAACGCGACCCGTCGCTGCGCGTGCTGCTGCTCGAGAAGGCGAACGTGAAGCGCAGCGGCGCGATCTCGATGGGGATGG
ACGGACTGAACAACGCCGTGATTCCGGGCCACGCGACGCCCGAACAGTACACGCGCGAGATCACGATCGCGAACGACGGC
ATCGTCGATCAGGAAGCGGTCCATGCGTATGCGACGCACAGCTTCGCGACGATCGAGCAGCTCGACCGCTGGGGCGTGAA
GTTCGAGAAGGACGGCACCGGCGACTATGCGGTGAAGAAGGTTCACCACATGGGGTCGTACGTGCTGCCGATGCCGGAAG
GTCACGACATCAAGAAGGTGCTGTATCGGCAGCTGAAGCGCGCGCGCATCGCGATCACGAACCGCATCGTCGCGACGCGC
GTGCTGACCGACGCGCGCGGCAACGCGAGTGGCGTGCTCGGCTTCGACTGCCGCACGGCCGAGTTCCACGTGATCCGCGC
AAAGGCCGTGATTCTGTGCTGCGGCGCGGCCGGCCGGCTCGGGCTGCCTGCGTCCGGCTATCTGATGGGTACCTACGAGA
ACCCGACGAACGCCGGCGACGGCTATGCGATGGCGTATCACGCGGGCGCCGCGCTCGCGAATCTCGAATGCTTCCAGATC
AATCCGCTGATCAAGGACTACAACGGTCCGGCCTGCGCGTACGTGACGGGCCCGCTCGGCGGCTTCACCGCGAACGGCAA
GGGCGAACGCTTCATCGAGTGCGATTACTGGAGCGGCCAGATGATGTGGGAGTTCTATGAGGAACTGCAAAGCGGCAACG
GCCCCGTGTTCCTGAAGCTCGACCATCTCGCCGAAGAGACGATCCGGACGATCGAGCAGATCCTGCATACGAACGAACGG
CCGAGCCGCGGACGCTTCCACGCGGGGCGCGGCACCGACTATCGGCAGCAGATGGTCGAGATGCACATCTCGGAGATCGG
CTTCTGCAGCGGCCACAGCGCCTCCGGCGTGTACGTGAACGCGCGCGCCGAGACGACCGTGCCGGGCCTCTACGCGGCCG
GCGACATGGCGGCGGTCCCGCACAACTACATGCTCGGCGCATTCACGTACGGCTGGTTCGCCGGACAGAACGCGGCCGAC
TACGTCGCGGGCCGCGAATTCGCGCCCGTCGATGCCGCGCAGGTCGACGCCGAACGCGCGCGTGTGTTCGCGCCGCTCGC
GCGCGAGCACGGCCTCGCGCCCGCTCAGGTCGAATACAAGCTGCGCCGGATGGTGAACGACTATCTGCAGCCGCCGAAGG
TGACGCGCAAGATGGAAATCGGGCTGCAGCGTTTTGCCGACATCGCTGAGGATATCGAAGCGATCAAGGCCACGCATCCG
CACGAGCTGATGCGCGCCGCCGAAGTGCGTGCGATCCGCGACTGCGCGGAAATGGCCGCGCGCGCGTCGCTGTTTCGTAC
CGAGAGCCGCTGGGGGCTGTATCACCATCGCGTCGACTATCCGCATCGCAACGATGCCGAATGGTTCTGCCACACGTGGC
TGCGCAAGCACGCGGACGGCACGATGCGCAGCGAGAAGCGGCCCGTCGAACCGTACATCGTGCCGCTCGCCGACGACGAG
CGCACCGCCTACGCGCAATTGCGCATCCGCGAACCGGCCGCCGTCGCGGCCGCTCTCTGA

Upstream 100 bases:

>100_bases
TCGATTACGAACACCTCTACTTCCGAGGCGACGCCTTCCAGTACCGGCTGCGCATCGACCGGCAGCGGCACAAGGCGCGC
AAACCTGCAAGGAATCCGCG

Downstream 100 bases:

>100_bases
GGAGCCCGCCCGTGTCCCATACCCCGCACGAGATCTTCCTGCGCAGCAGCGCGCCGGTCACGATCGACGAGGAACGCTGC
ATCGCCGACAAGGGCTGCAC

Product: putative oxidoreductase

Products: FADH2; Fumarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MNTHVLEYDIVVVGGGTAGPMAAIKAKERDPSLRVLLLEKANVKRSGAISMGMDGLNNAVIPGHATPEQYTREITIANDG
IVDQEAVHAYATHSFATIEQLDRWGVKFEKDGTGDYAVKKVHHMGSYVLPMPEGHDIKKVLYRQLKRARIAITNRIVATR
VLTDARGNASGVLGFDCRTAEFHVIRAKAVILCCGAAGRLGLPASGYLMGTYENPTNAGDGYAMAYHAGAALANLECFQI
NPLIKDYNGPACAYVTGPLGGFTANGKGERFIECDYWSGQMMWEFYEELQSGNGPVFLKLDHLAEETIRTIEQILHTNER
PSRGRFHAGRGTDYRQQMVEMHISEIGFCSGHSASGVYVNARAETTVPGLYAAGDMAAVPHNYMLGAFTYGWFAGQNAAD
YVAGREFAPVDAAQVDAERARVFAPLAREHGLAPAQVEYKLRRMVNDYLQPPKVTRKMEIGLQRFADIAEDIEAIKATHP
HELMRAAEVRAIRDCAEMAARASLFRTESRWGLYHHRVDYPHRNDAEWFCHTWLRKHADGTMRSEKRPVEPYIVPLADDE
RTAYAQLRIREPAAVAAAL

Sequences:

>Translated_579_residues
MNTHVLEYDIVVVGGGTAGPMAAIKAKERDPSLRVLLLEKANVKRSGAISMGMDGLNNAVIPGHATPEQYTREITIANDG
IVDQEAVHAYATHSFATIEQLDRWGVKFEKDGTGDYAVKKVHHMGSYVLPMPEGHDIKKVLYRQLKRARIAITNRIVATR
VLTDARGNASGVLGFDCRTAEFHVIRAKAVILCCGAAGRLGLPASGYLMGTYENPTNAGDGYAMAYHAGAALANLECFQI
NPLIKDYNGPACAYVTGPLGGFTANGKGERFIECDYWSGQMMWEFYEELQSGNGPVFLKLDHLAEETIRTIEQILHTNER
PSRGRFHAGRGTDYRQQMVEMHISEIGFCSGHSASGVYVNARAETTVPGLYAAGDMAAVPHNYMLGAFTYGWFAGQNAAD
YVAGREFAPVDAAQVDAERARVFAPLAREHGLAPAQVEYKLRRMVNDYLQPPKVTRKMEIGLQRFADIAEDIEAIKATHP
HELMRAAEVRAIRDCAEMAARASLFRTESRWGLYHHRVDYPHRNDAEWFCHTWLRKHADGTMRSEKRPVEPYIVPLADDE
RTAYAQLRIREPAAVAAAL
>Mature_579_residues
MNTHVLEYDIVVVGGGTAGPMAAIKAKERDPSLRVLLLEKANVKRSGAISMGMDGLNNAVIPGHATPEQYTREITIANDG
IVDQEAVHAYATHSFATIEQLDRWGVKFEKDGTGDYAVKKVHHMGSYVLPMPEGHDIKKVLYRQLKRARIAITNRIVATR
VLTDARGNASGVLGFDCRTAEFHVIRAKAVILCCGAAGRLGLPASGYLMGTYENPTNAGDGYAMAYHAGAALANLECFQI
NPLIKDYNGPACAYVTGPLGGFTANGKGERFIECDYWSGQMMWEFYEELQSGNGPVFLKLDHLAEETIRTIEQILHTNER
PSRGRFHAGRGTDYRQQMVEMHISEIGFCSGHSASGVYVNARAETTVPGLYAAGDMAAVPHNYMLGAFTYGWFAGQNAAD
YVAGREFAPVDAAQVDAERARVFAPLAREHGLAPAQVEYKLRRMVNDYLQPPKVTRKMEIGLQRFADIAEDIEAIKATHP
HELMRAAEVRAIRDCAEMAARASLFRTESRWGLYHHRVDYPHRNDAEWFCHTWLRKHADGTMRSEKRPVEPYIVPLADDE
RTAYAQLRIREPAAVAAAL

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786942, Length=603, Percent_Identity=24.7097844112769, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790597, Length=605, Percent_Identity=22.1487603305785, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: 1900 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1360 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1100 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.3.99.1 [C]

Molecular weight: Translated: 64055; Mature: 64055

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTHVLEYDIVVVGGGTAGPMAAIKAKERDPSLRVLLLEKANVKRSGAISMGMDGLNNAV
CCCEEEEEEEEEECCCCCCCHHHEECCCCCCCEEEEEEECCCCCCCCCEEECCCCCCCCC
IPGHATPEQYTREITIANDGIVDQEAVHAYATHSFATIEQLDRWGVKFEKDGTGDYAVKK
CCCCCCHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHH
VHHMGSYVLPMPEGHDIKKVLYRQLKRARIAITNRIVATRVLTDARGNASGVLGFDCRTA
HHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCC
EFHVIRAKAVILCCGAAGRLGLPASGYLMGTYENPTNAGDGYAMAYHAGAALANLECFQI
EEEEEEEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCHHHCEEEEEE
NPLIKDYNGPACAYVTGPLGGFTANGKGERFIECDYWSGQMMWEFYEELQSGNGPVFLKL
CCHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEE
DHLAEETIRTIEQILHTNERPSRGRFHAGRGTDYRQQMVEMHISEIGFCSGHSASGVYVN
HHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEE
ARAETTVPGLYAAGDMAAVPHNYMLGAFTYGWFAGQNAADYVAGREFAPVDAAQVDAERA
CCCCCCCCCEEECCCCCCCCCCCEEEEEEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHH
RVFAPLAREHGLAPAQVEYKLRRMVNDYLQPPKVTRKMEIGLQRFADIAEDIEAIKATHP
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
HELMRAAEVRAIRDCAEMAARASLFRTESRWGLYHHRVDYPHRNDAEWFCHTWLRKHADG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCC
TMRSEKRPVEPYIVPLADDERTAYAQLRIREPAAVAAAL
CCCCCCCCCCCEEEEECCCCCCEEEEEEECCCHHHHHCC
>Mature Secondary Structure
MNTHVLEYDIVVVGGGTAGPMAAIKAKERDPSLRVLLLEKANVKRSGAISMGMDGLNNAV
CCCEEEEEEEEEECCCCCCCHHHEECCCCCCCEEEEEEECCCCCCCCCEEECCCCCCCCC
IPGHATPEQYTREITIANDGIVDQEAVHAYATHSFATIEQLDRWGVKFEKDGTGDYAVKK
CCCCCCHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHH
VHHMGSYVLPMPEGHDIKKVLYRQLKRARIAITNRIVATRVLTDARGNASGVLGFDCRTA
HHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCC
EFHVIRAKAVILCCGAAGRLGLPASGYLMGTYENPTNAGDGYAMAYHAGAALANLECFQI
EEEEEEEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCHHHCEEEEEE
NPLIKDYNGPACAYVTGPLGGFTANGKGERFIECDYWSGQMMWEFYEELQSGNGPVFLKL
CCHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEE
DHLAEETIRTIEQILHTNERPSRGRFHAGRGTDYRQQMVEMHISEIGFCSGHSASGVYVN
HHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEE
ARAETTVPGLYAAGDMAAVPHNYMLGAFTYGWFAGQNAADYVAGREFAPVDAAQVDAERA
CCCCCCCCCEEECCCCCCCCCCCEEEEEEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHH
RVFAPLAREHGLAPAQVEYKLRRMVNDYLQPPKVTRKMEIGLQRFADIAEDIEAIKATHP
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
HELMRAAEVRAIRDCAEMAARASLFRTESRWGLYHHRVDYPHRNDAEWFCHTWLRKHADG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCC
TMRSEKRPVEPYIVPLADDERTAYAQLRIREPAAVAAAL
CCCCCCCCCCCEEEEECCCCCCEEEEEEECCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]

Metal ions: Anions; Diphosphate; Fe [C]

Kcat value (1/min): 10000-13000 [C]

Specific activity: 509

Km value (mM): 0.02 {succinate}} 0.4 {fumarate}} 0.3 {ferricyanide}} [C]

Substrates: FAD; Succinate [C]

Specific reaction: FAD + Succinate --> FADH2 + Fumarate [C]

General reaction: Redox reaction [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]