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The map label for this gene is lpdA [H]

Identifier: 161524310

GI number: 161524310

Start: 1252260

End: 1254029

Strand: Direct

Name: lpdA [H]

Synonym: Bmul_1135

Alternate gene names: 161524310

Gene position: 1252260-1254029 (Clockwise)

Preceding gene: 161524309

Following gene: 161524312

Centisome position: 36.31

GC content: 65.59

Gene sequence:

>1770_bases
ATGAGTCTCATCGAAGTCAAGGTTCCGGATATCGGCGATTTCAGCGGCGTCGATGTCATCGAAGTCAACGTGAAGCCGGG
CGACGTGATCGAAAAAGAGCAGACGCTCATCACGCTCGAATCGGACAAGGCCTCCATGGAAGTGCCGAGCGACGTCGCCG
GCACCGTCAAGGAAATCAAGATCAAGGCCGGCGACAAGGTCTCGCAAGGCACGGTCATCGCGCTCGTCGAAGCAGCGGAA
GGCGCAGCCGCACCGGCGAAAGCGCCGGAGCCGGCCAAGCCCGCCGCAGCCGCGCCGGCGGCCGCCCCCGCAGCCGCATC
GGCACCCGCGCCGCAAGCGGGCAGCTATGCGGGCGCAGCCGACATCGAGTGCGACATGCTCGTGCTCGGCGCCGGCCCCG
GCGGCTACTCGGCCGCGTTCCGCGCGGCCGACCTCGGCATGAAGACGGTGCTCGTCGAACGCTACCCGACGCTCGGCGGC
GTGTGCCTGAACGTCGGCTGCATCCCGTCGAAAGCGCTGCTGCATACGGCGCTCGTCGTCGAGGAAGCCGAGGCGCTCGC
CGCGCACGGCATCTCGTTCGGCAAGCCGCAGGTCGACCTCGACAAGCTGCGCGACTTCAAGGGCGGCGTCGTCAAGAAAC
TGACGACGGGCCTCGCCGGCATGGCAAAGGCGCGCAAGGTCGAAGTCGTGACCGGCGTCGGCACGTTCGTCGATCCGCAC
CACATGGAAGTGCAGGGCGAAAACGGCAAGAAGGTCGTCAAGTTCAAGCAGGCGATCATCGCCGCGGGCTCGCAGGCCTT
GAAGCTGCCGTTCATGCCGGAAGATCCGCGCGTCGTCGATTCGACCGGCGCGCTCGAACTGCGCCAGCTGCCGAAGCGCA
TGCTCGTGATCGGCGGCGGCATCATCGGCCTCGAAATGGCGACGGTCTATTCGACGCTCGGTGCCGAAATCGACGTCGTC
GAAATGATGGACGGCCTGATGATGGGCGCAGACCGCGATCTCGTGAAGGTCTGGGAAAAGTACAACGCGAAGCGCTTCGG
CAACGTGATGCTGAAGACCAAGACAGTCGGCGCGGAAGCGAAGGAAGACGGCATCTACGTGAAGTTCGAAGGCGAGAAGG
CGCCGGCGGAACCGCAGCGCTACGACCTCGTGCTCGTCGCGGTCGGCCGCAGCCCGAACGGTAAGAAGATCGGTGCCGAC
AAGGCCGGCGTCGCCGTCACGGACCGCGGCTTCATCGAAGTCGACAAGCAGATGCGCACGAACGTCCCGCACATCTTCGC
GATCGGCGACATCGTCGGCCAGCCGATGCTCGCGCACAAGGCCGTCCATGAAGGCCACGTCGCCGCCGAAGCCGCACACG
GCGAAAAGGCGTACTTCGACGCGCTGCAGATTCCGTCGGTCGCCTATACCGATCCGGAAGTCGCGTGGGCCGGCAAGACG
GAAGACCAGTGCAAGGCCGAAGGCATCAAGTACGGCAAGGCCGTGTTCCCGTGGGCCGCATCGGGCCGCGCGATCGCGAA
CGGCCGCGACGAAGGCTTCACGAAGCTGATCTTCGACGAGGAGACGCATCGCGTGATCGGCGGCGGTATCGTCGGCCTGA
ACGCGGGCGATCTGATCAGCGAAGTATGCCTCGCGGTCGAAATGGGCGCGGACGCCGAAGACATCGGCAAGACGATTCAC
CCGCACCCGACGCTCGGCGAATCGGTCGGCATGGCTGCCGAACTGTACGAAGGCGTCTGCACGGACCTGCCGCCGCAGCG
CAAGAAGTAA

Upstream 100 bases:

>100_bases
GGTGGTGCTGCCCAGGTTCGCGTTCTTTGGTCAGGCCGTAAGTGCGGTTCTCCGCGGGGTTCTCCCTCTCTGTCTGCAGC
TAATCAAGAAGGGGACAGTA

Downstream 100 bases:

>100_bases
CGCGCACGACCGGCCCCACGCCGGTCGAGACGAACGCCGCCTGTCCGGGCTCTCCGGCAGGCGGCGTTTTTCGTTGGATC
GGGCGCCGGCCGCGCCGGTC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 589; Mature: 588

Protein sequence:

>589_residues
MSLIEVKVPDIGDFSGVDVIEVNVKPGDVIEKEQTLITLESDKASMEVPSDVAGTVKEIKIKAGDKVSQGTVIALVEAAE
GAAAPAKAPEPAKPAAAAPAAAPAAASAPAPQAGSYAGAADIECDMLVLGAGPGGYSAAFRAADLGMKTVLVERYPTLGG
VCLNVGCIPSKALLHTALVVEEAEALAAHGISFGKPQVDLDKLRDFKGGVVKKLTTGLAGMAKARKVEVVTGVGTFVDPH
HMEVQGENGKKVVKFKQAIIAAGSQALKLPFMPEDPRVVDSTGALELRQLPKRMLVIGGGIIGLEMATVYSTLGAEIDVV
EMMDGLMMGADRDLVKVWEKYNAKRFGNVMLKTKTVGAEAKEDGIYVKFEGEKAPAEPQRYDLVLVAVGRSPNGKKIGAD
KAGVAVTDRGFIEVDKQMRTNVPHIFAIGDIVGQPMLAHKAVHEGHVAAEAAHGEKAYFDALQIPSVAYTDPEVAWAGKT
EDQCKAEGIKYGKAVFPWAASGRAIANGRDEGFTKLIFDEETHRVIGGGIVGLNAGDLISEVCLAVEMGADAEDIGKTIH
PHPTLGESVGMAAELYEGVCTDLPPQRKK

Sequences:

>Translated_589_residues
MSLIEVKVPDIGDFSGVDVIEVNVKPGDVIEKEQTLITLESDKASMEVPSDVAGTVKEIKIKAGDKVSQGTVIALVEAAE
GAAAPAKAPEPAKPAAAAPAAAPAAASAPAPQAGSYAGAADIECDMLVLGAGPGGYSAAFRAADLGMKTVLVERYPTLGG
VCLNVGCIPSKALLHTALVVEEAEALAAHGISFGKPQVDLDKLRDFKGGVVKKLTTGLAGMAKARKVEVVTGVGTFVDPH
HMEVQGENGKKVVKFKQAIIAAGSQALKLPFMPEDPRVVDSTGALELRQLPKRMLVIGGGIIGLEMATVYSTLGAEIDVV
EMMDGLMMGADRDLVKVWEKYNAKRFGNVMLKTKTVGAEAKEDGIYVKFEGEKAPAEPQRYDLVLVAVGRSPNGKKIGAD
KAGVAVTDRGFIEVDKQMRTNVPHIFAIGDIVGQPMLAHKAVHEGHVAAEAAHGEKAYFDALQIPSVAYTDPEVAWAGKT
EDQCKAEGIKYGKAVFPWAASGRAIANGRDEGFTKLIFDEETHRVIGGGIVGLNAGDLISEVCLAVEMGADAEDIGKTIH
PHPTLGESVGMAAELYEGVCTDLPPQRKK
>Mature_588_residues
SLIEVKVPDIGDFSGVDVIEVNVKPGDVIEKEQTLITLESDKASMEVPSDVAGTVKEIKIKAGDKVSQGTVIALVEAAEG
AAAPAKAPEPAKPAAAAPAAAPAAASAPAPQAGSYAGAADIECDMLVLGAGPGGYSAAFRAADLGMKTVLVERYPTLGGV
CLNVGCIPSKALLHTALVVEEAEALAAHGISFGKPQVDLDKLRDFKGGVVKKLTTGLAGMAKARKVEVVTGVGTFVDPHH
MEVQGENGKKVVKFKQAIIAAGSQALKLPFMPEDPRVVDSTGALELRQLPKRMLVIGGGIIGLEMATVYSTLGAEIDVVE
MMDGLMMGADRDLVKVWEKYNAKRFGNVMLKTKTVGAEAKEDGIYVKFEGEKAPAEPQRYDLVLVAVGRSPNGKKIGADK
AGVAVTDRGFIEVDKQMRTNVPHIFAIGDIVGQPMLAHKAVHEGHVAAEAAHGEKAYFDALQIPSVAYTDPEVAWAGKTE
DQCKAEGIKYGKAVFPWAASGRAIANGRDEGFTKLIFDEETHRVIGGGIVGLNAGDLISEVCLAVEMGADAEDIGKTIHP
HPTLGESVGMAAELYEGVCTDLPPQRKK

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=454, Percent_Identity=41.4096916299559, Blast_Score=333, Evalue=3e-91,
Organism=Homo sapiens, GI50301238, Length=469, Percent_Identity=27.9317697228145, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI291045266, Length=432, Percent_Identity=26.8518518518519, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI148277065, Length=426, Percent_Identity=25.3521126760563, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI148277071, Length=426, Percent_Identity=25.3521126760563, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI33519430, Length=426, Percent_Identity=25.3521126760563, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI33519428, Length=426, Percent_Identity=25.3521126760563, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI33519426, Length=426, Percent_Identity=25.3521126760563, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI22035672, Length=454, Percent_Identity=27.0925110132159, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI291045268, Length=425, Percent_Identity=25.4117647058824, Blast_Score=113, Evalue=6e-25,
Organism=Escherichia coli, GI1786307, Length=473, Percent_Identity=66.8076109936575, Blast_Score=639, Evalue=0.0,
Organism=Escherichia coli, GI87082354, Length=463, Percent_Identity=28.7257019438445, Blast_Score=178, Evalue=9e-46,
Organism=Escherichia coli, GI87081717, Length=450, Percent_Identity=25.3333333333333, Blast_Score=156, Evalue=4e-39,
Organism=Escherichia coli, GI1789915, Length=444, Percent_Identity=28.3783783783784, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1786305, Length=73, Percent_Identity=60.2739726027397, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI32565766, Length=461, Percent_Identity=42.5162689804772, Blast_Score=347, Evalue=7e-96,
Organism=Caenorhabditis elegans, GI17557007, Length=479, Percent_Identity=27.7661795407098, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71983429, Length=459, Percent_Identity=26.3616557734205, Blast_Score=113, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71983419, Length=459, Percent_Identity=26.3616557734205, Blast_Score=113, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71982272, Length=439, Percent_Identity=25.5125284738041, Blast_Score=108, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6321091, Length=457, Percent_Identity=40.0437636761488, Blast_Score=303, Evalue=7e-83,
Organism=Saccharomyces cerevisiae, GI6325240, Length=470, Percent_Identity=28.2978723404255, Blast_Score=190, Evalue=6e-49,
Organism=Saccharomyces cerevisiae, GI6325166, Length=465, Percent_Identity=26.8817204301075, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI21358499, Length=479, Percent_Identity=42.7974947807933, Blast_Score=358, Evalue=6e-99,
Organism=Drosophila melanogaster, GI24640551, Length=512, Percent_Identity=28.7109375, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24640549, Length=480, Percent_Identity=28.75, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24640553, Length=482, Percent_Identity=28.4232365145228, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI17737741, Length=470, Percent_Identity=25.7446808510638, Blast_Score=114, Evalue=2e-25,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 61637; Mature: 61505

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLIEVKVPDIGDFSGVDVIEVNVKPGDVIEKEQTLITLESDKASMEVPSDVAGTVKEIK
CCEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCHHHCCEEEEE
IKAGDKVSQGTVIALVEAAEGAAAPAKAPEPAKPAAAAPAAAPAAASAPAPQAGSYAGAA
EECCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DIECDMLVLGAGPGGYSAAFRAADLGMKTVLVERYPTLGGVCLNVGCIPSKALLHTALVV
CCEEEEEEEECCCCCHHHHHHHHHCCHHEEHHHHCCCCCCEEEEECCCCCHHHHHHHHHH
EEAEALAAHGISFGKPQVDLDKLRDFKGGVVKKLTTGLAGMAKARKVEVVTGVGTFVDPH
HHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCC
HMEVQGENGKKVVKFKQAIIAAGSQALKLPFMPEDPRVVDSTGALELRQLPKRMLVIGGG
EEEEECCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCEECCCCCHHHHHCCCEEEEECCC
IIGLEMATVYSTLGAEIDVVEMMDGLMMGADRDLVKVWEKYNAKRFGNVMLKTKTVGAEA
CHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCHHHHHHHHHHCHHHHCCEEEEEEECCCCC
KEDGIYVKFEGEKAPAEPQRYDLVLVAVGRSPNGKKIGADKAGVAVTDRGFIEVDKQMRT
CCCCEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEECCCCEEECHHHHC
NVPHIFAIGDIVGQPMLAHKAVHEGHVAAEAAHGEKAYFDALQIPSVAYTDPEVAWAGKT
CCCEEEEEHHHHCCCHHHHHHHHCCCCEEECCCCCHHHHHHHCCCCEEECCCCEEECCCC
EDQCKAEGIKYGKAVFPWAASGRAIANGRDEGFTKLIFDEETHRVIGGGIVGLNAGDLIS
HHHHHHCCCCCCCEECCCCCCCCEEECCCCCCCEEEEECCCCCEEECCCEEECCHHHHHH
EVCLAVEMGADAEDIGKTIHPHPTLGESVGMAAELYEGVCTDLPPQRKK
HHHHHHHCCCCHHHHCCCCCCCCCCCHHHCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SLIEVKVPDIGDFSGVDVIEVNVKPGDVIEKEQTLITLESDKASMEVPSDVAGTVKEIK
CEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCHHHCCEEEEE
IKAGDKVSQGTVIALVEAAEGAAAPAKAPEPAKPAAAAPAAAPAAASAPAPQAGSYAGAA
EECCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DIECDMLVLGAGPGGYSAAFRAADLGMKTVLVERYPTLGGVCLNVGCIPSKALLHTALVV
CCEEEEEEEECCCCCHHHHHHHHHCCHHEEHHHHCCCCCCEEEEECCCCCHHHHHHHHHH
EEAEALAAHGISFGKPQVDLDKLRDFKGGVVKKLTTGLAGMAKARKVEVVTGVGTFVDPH
HHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCC
HMEVQGENGKKVVKFKQAIIAAGSQALKLPFMPEDPRVVDSTGALELRQLPKRMLVIGGG
EEEEECCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCEECCCCCHHHHHCCCEEEEECCC
IIGLEMATVYSTLGAEIDVVEMMDGLMMGADRDLVKVWEKYNAKRFGNVMLKTKTVGAEA
CHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCHHHHHHHHHHCHHHHCCEEEEEEECCCCC
KEDGIYVKFEGEKAPAEPQRYDLVLVAVGRSPNGKKIGADKAGVAVTDRGFIEVDKQMRT
CCCCEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEECCCCEEECHHHHC
NVPHIFAIGDIVGQPMLAHKAVHEGHVAAEAAHGEKAYFDALQIPSVAYTDPEVAWAGKT
CCCEEEEEHHHHCCCHHHHHHHHCCCCEEECCCCCHHHHHHHCCCCEEECCCCEEECCCC
EDQCKAEGIKYGKAVFPWAASGRAIANGRDEGFTKLIFDEETHRVIGGGIVGLNAGDLIS
HHHHHHCCCCCCCEECCCCCCCCEEECCCCCCCEEEEECCCCCEEECCCEEECCHHHHHH
EVCLAVEMGADAEDIGKTIHPHPTLGESVGMAAELYEGVCTDLPPQRKK
HHHHHHHCCCCHHHHCCCCCCCCCCCHHHCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]