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The map label for this gene is pdhB [H]

Identifier: 161524309

GI number: 161524309

Start: 1250311

End: 1251978

Strand: Direct

Name: pdhB [H]

Synonym: Bmul_1134

Alternate gene names: 161524309

Gene position: 1250311-1251978 (Clockwise)

Preceding gene: 161524308

Following gene: 161524310

Centisome position: 36.26

GC content: 68.59

Gene sequence:

>1668_bases
ATGAGTCAAGCGATCGAAGTGAAGGTGCCGGATATCGGCGATTACAAGGACGTGCCCGTCATCGAGGTGCTCGTGAAAGC
GGGCGACACGGTCGAGCCCGAGCAGTCGCTCGTCACGCTCGAGTCCGACAAGGCGACGATGGACGTGCCGAGCCCCGCCG
CGGGCACGGTCAAGGAAGTGAAGGTCAAGGTGGGCGACGCCGTGTCGGAAGGCACGCTGATCATCCTGCTCGAAGGCGGC
GCCGCCGCGCAGCCGAACGGCGCCGCTGCGCCGGCGGCAGCACCGGCCCAGGCGTCCGCGCCGGCACCGGCTGCCGCGGC
CCCCGCGGCTGCCGCACCGGCCGCCGCGCCCGCCGCATCGGGCGGCACGCTCGAAGTGAAGGTGCCCGACATCGGCGACT
ACAAGGACGTGCCGGTGATCGAGATCGGCGTGAAGGTCGGCGACACGGTCGAGAAGGAGCAGTCGCTCGTCACGCTCGAA
TCGGACAAGGCGACGATGGACGTGCCGAGCCCGGCCGCCGGCGTCGTGAAGGACATCAAGGTGAAGGTCGGCGACACCGT
GTCGGAAGGCACGCTGATCGTGCTGCTCGAGGCCGCCGGCGCAGCGCCGGCCGCTGCGGCGCCGCAGGCGAGCGCACCGG
CGCCGGCACCGGCTGCCGCCGCTCCGGCTGCCGCCCCCGCACCGGCACCGGCGAAGGCCGCGGCACCGGCTGCCGCATCG
GCCGCTGCCGCGCCGTCGGGCGAACACCGTGCGAGCCACGCGTCGCCGTCGGTGCGCAAGTTCGCGCGCGAACTGGGCGT
TGACGTGTCGCGCGTGCAGGGCAGCGGTCCGAAGGGCCGCATCACGAAGGAAGACGTCACGGCGTTCGTGAAGGGCGTCA
TGACGGGCCAGCAGGCGGCGCCGGCCGCAGCGGCCGCGCTGGCCGGCGGCGGCGAGCTGAACCTGCTGCCGTGGCCGAAG
GTCGACTTCTCGAAGTTCGGCCCGTTCGAGTCGAAGCCGCTGTCGCGCATCAAGAAGATCTCGGGCGCGAACCTGCATCG
CAACTGGGTGATGATCCCGCACGTCACGAACAACGACGAAGCCGACATCACCGATCTCGAAGCGCTGCGCGTGCAGCTGA
ACAAGGAGCACGAGAAGGCGGGCGTGAAGTTCACGATGCTCGCATTCGTGATCAAGGCGGTCGTCGCCGCGCTGAAGAAG
TTCCCGACCTTCAACGCGAGCCTCGACGGCGACAACCTGGTGTTCAAGCAGTACTACCACATCGGTTTCGCGGCCGACAC
GCCGAACGGTCTCGTCGTGCCGGTGATCCGCGATGCGGACAAGAAGGGCCTCGTCGAAATCGCGAAGGAAATGGCGGAGC
TGTCGAAGGCCGCGCGCGAAGGCAAGCTGAAGCCGGACCAGATGCAGGGCGGCTGCTTCTCGATCTCGTCGCTCGGCGGG
ATCGGCGGCACGCATTTCACGCCGATCATCAACGCGCCGGAAGTCGCGATCCTCGGCTTGTCGCGCGGCCAGATGAAGCC
GGTGTGGGACGGCAAGCAGTTCGTGCCGCGTCTGACGCTGCCGCTGTCGCTGTCGTATGACCATCGTGTGATCGATGGCG
CGGAGGCTGCGCGGTTCAATGCGTATCTCGGCGCGTTGCTCGCGGATTTCCGTCGCATCATTCTTTGA

Upstream 100 bases:

>100_bases
GATCCGTCGAAGCCGAACCCGATGACCGTTTAACGACACGCGCCGCGTGATGCCGCGCGCCGCCTCGCGGGGAGGCGCGC
GGTCCTGGAGACACAGACAG

Downstream 100 bases:

>100_bases
TGAGCGGGGAGAGTGCTCGCGGGGGCGTGGGTTTTGTTCGGTCGGTTATCCGCGCTCTGCCTTTGAACCTGTATTGCGAA
CGGTCTGCTAGCGTCGCCCC

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MSQAIEVKVPDIGDYKDVPVIEVLVKAGDTVEPEQSLVTLESDKATMDVPSPAAGTVKEVKVKVGDAVSEGTLIILLEGG
AAAQPNGAAAPAAAPAQASAPAPAAAAPAAAAPAAAPAASGGTLEVKVPDIGDYKDVPVIEIGVKVGDTVEKEQSLVTLE
SDKATMDVPSPAAGVVKDIKVKVGDTVSEGTLIVLLEAAGAAPAAAAPQASAPAPAPAAAAPAAAPAPAPAKAAAPAAAS
AAAAPSGEHRASHASPSVRKFARELGVDVSRVQGSGPKGRITKEDVTAFVKGVMTGQQAAPAAAAALAGGGELNLLPWPK
VDFSKFGPFESKPLSRIKKISGANLHRNWVMIPHVTNNDEADITDLEALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKK
FPTFNASLDGDNLVFKQYYHIGFAADTPNGLVVPVIRDADKKGLVEIAKEMAELSKAAREGKLKPDQMQGGCFSISSLGG
IGGTHFTPIINAPEVAILGLSRGQMKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLGALLADFRRIIL

Sequences:

>Translated_555_residues
MSQAIEVKVPDIGDYKDVPVIEVLVKAGDTVEPEQSLVTLESDKATMDVPSPAAGTVKEVKVKVGDAVSEGTLIILLEGG
AAAQPNGAAAPAAAPAQASAPAPAAAAPAAAAPAAAPAASGGTLEVKVPDIGDYKDVPVIEIGVKVGDTVEKEQSLVTLE
SDKATMDVPSPAAGVVKDIKVKVGDTVSEGTLIVLLEAAGAAPAAAAPQASAPAPAPAAAAPAAAPAPAPAKAAAPAAAS
AAAAPSGEHRASHASPSVRKFARELGVDVSRVQGSGPKGRITKEDVTAFVKGVMTGQQAAPAAAAALAGGGELNLLPWPK
VDFSKFGPFESKPLSRIKKISGANLHRNWVMIPHVTNNDEADITDLEALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKK
FPTFNASLDGDNLVFKQYYHIGFAADTPNGLVVPVIRDADKKGLVEIAKEMAELSKAAREGKLKPDQMQGGCFSISSLGG
IGGTHFTPIINAPEVAILGLSRGQMKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLGALLADFRRIIL
>Mature_554_residues
SQAIEVKVPDIGDYKDVPVIEVLVKAGDTVEPEQSLVTLESDKATMDVPSPAAGTVKEVKVKVGDAVSEGTLIILLEGGA
AAQPNGAAAPAAAPAQASAPAPAAAAPAAAAPAAAPAASGGTLEVKVPDIGDYKDVPVIEIGVKVGDTVEKEQSLVTLES
DKATMDVPSPAAGVVKDIKVKVGDTVSEGTLIVLLEAAGAAPAAAAPQASAPAPAPAAAAPAAAPAPAPAKAAAPAAASA
AAAPSGEHRASHASPSVRKFARELGVDVSRVQGSGPKGRITKEDVTAFVKGVMTGQQAAPAAAAALAGGGELNLLPWPKV
DFSKFGPFESKPLSRIKKISGANLHRNWVMIPHVTNNDEADITDLEALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKF
PTFNASLDGDNLVFKQYYHIGFAADTPNGLVVPVIRDADKKGLVEIAKEMAELSKAAREGKLKPDQMQGGCFSISSLGGI
GGTHFTPIINAPEVAILGLSRGQMKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLGALLADFRRIIL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=446, Percent_Identity=30.0448430493274, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI31711992, Length=419, Percent_Identity=33.4128878281623, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI203098816, Length=512, Percent_Identity=26.953125, Blast_Score=136, Evalue=6e-32,
Organism=Homo sapiens, GI203098753, Length=434, Percent_Identity=27.4193548387097, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI19923748, Length=227, Percent_Identity=33.920704845815, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI260898739, Length=153, Percent_Identity=37.9084967320261, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1786305, Length=554, Percent_Identity=55.0541516245487, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI1786946, Length=435, Percent_Identity=30.1149425287356, Blast_Score=187, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17537937, Length=421, Percent_Identity=28.0285035629454, Blast_Score=177, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17560088, Length=445, Percent_Identity=30.7865168539326, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI25146366, Length=205, Percent_Identity=38.0487804878049, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17538894, Length=317, Percent_Identity=31.5457413249211, Blast_Score=119, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6320352, Length=417, Percent_Identity=30.2158273381295, Blast_Score=180, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6324258, Length=432, Percent_Identity=29.3981481481481, Blast_Score=140, Evalue=4e-34,
Organism=Drosophila melanogaster, GI18859875, Length=444, Percent_Identity=31.3063063063063, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24582497, Length=232, Percent_Identity=31.8965517241379, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI20129315, Length=232, Percent_Identity=31.8965517241379, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24645909, Length=207, Percent_Identity=34.7826086956522, Blast_Score=118, Evalue=1e-26,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 56839; Mature: 56707

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQAIEVKVPDIGDYKDVPVIEVLVKAGDTVEPEQSLVTLESDKATMDVPSPAAGTVKEV
CCCEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCEECCCCCCCCCCEEE
KVKVGDAVSEGTLIILLEGGAAAQPNGAAAPAAAPAQASAPAPAAAAPAAAAPAAAPAAS
EEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GGTLEVKVPDIGDYKDVPVIEIGVKVGDTVEKEQSLVTLESDKATMDVPSPAAGVVKDIK
CCEEEEECCCCCCCCCCCEEEECCCCCCCHHCCCCEEEEECCCCEECCCCCHHHHHHHEE
VKVGDTVSEGTLIVLLEAAGAAPAAAAPQASAPAPAPAAAAPAAAPAPAPAKAAAPAAAS
EEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHH
AAAAPSGEHRASHASPSVRKFARELGVDVSRVQGSGPKGRITKEDVTAFVKGVMTGQQAA
HCCCCCCCCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCC
PAAAAALAGGGELNLLPWPKVDFSKFGPFESKPLSRIKKISGANLHRNWVMIPHVTNNDE
CHHHHHHCCCCCEEECCCCCCCHHHCCCCCCCHHHHHHHHCCCCEECCEEEEEEECCCCC
ADITDLEALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNASLDGDNLVFKQYYH
CCHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEE
IGFAADTPNGLVVPVIRDADKKGLVEIAKEMAELSKAAREGKLKPDQMQGGCFSISSLGG
EEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEECCCCC
IGGTHFTPIINAPEVAILGLSRGQMKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFN
CCCCCCCEECCCCCEEEEECCCCCCCCCCCCHHHCCEEEEEEEECCCCEEECCCHHHHHH
AYLGALLADFRRIIL
HHHHHHHHHHHHHHC
>Mature Secondary Structure 
SQAIEVKVPDIGDYKDVPVIEVLVKAGDTVEPEQSLVTLESDKATMDVPSPAAGTVKEV
CCEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCEECCCCCCCCCCEEE
KVKVGDAVSEGTLIILLEGGAAAQPNGAAAPAAAPAQASAPAPAAAAPAAAAPAAAPAAS
EEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GGTLEVKVPDIGDYKDVPVIEIGVKVGDTVEKEQSLVTLESDKATMDVPSPAAGVVKDIK
CCEEEEECCCCCCCCCCCEEEECCCCCCCHHCCCCEEEEECCCCEECCCCCHHHHHHHEE
VKVGDTVSEGTLIVLLEAAGAAPAAAAPQASAPAPAPAAAAPAAAPAPAPAKAAAPAAAS
EEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHH
AAAAPSGEHRASHASPSVRKFARELGVDVSRVQGSGPKGRITKEDVTAFVKGVMTGQQAA
HCCCCCCCCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCC
PAAAAALAGGGELNLLPWPKVDFSKFGPFESKPLSRIKKISGANLHRNWVMIPHVTNNDE
CHHHHHHCCCCCEEECCCCCCCHHHCCCCCCCHHHHHHHHCCCCEECCEEEEEEECCCCC
ADITDLEALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNASLDGDNLVFKQYYH
CCHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEE
IGFAADTPNGLVVPVIRDADKKGLVEIAKEMAELSKAAREGKLKPDQMQGGCFSISSLGG
EEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEECCCCC
IGGTHFTPIINAPEVAILGLSRGQMKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFN
CCCCCCCEECCCCCEEEEECCCCCCCCCCCCHHHCCEEEEEEEECCCCEEECCCHHHHHH
AYLGALLADFRRIIL
HHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8021225 [H]