Click here to switch to the map view.

The map label for this gene is yfcG [H]

Identifier: 161524225

GI number: 161524225

Start: 1144687

End: 1145379

Strand: Reverse

Name: yfcG [H]

Synonym: Bmul_1049

Alternate gene names: 161524225

Gene position: 1145379-1144687 (Counterclockwise)

Preceding gene: 161524226

Following gene: 161524197

Centisome position: 33.21

GC content: 64.07

Gene sequence:

>693_bases
ATGATCGACGTCTACAGCTGGGCGACCCCGAACGGTCACAAGGTGCACATCATGCTCGAGGAAACCGGCCTCGCGTATCG
CGCGCATCCGATCGATATCGGCGCGGGCGACCAGTTCAACGCCGAGTTCCTGAAGATCAGTCCGAACAACAAGATCCCCG
CGATCGTCGACGACGAAGGCCCGGGCGGGCAGCCGATCTCGCTGTTCGAATCGGGCGCGATCCTGATCTATCTCGCGGAG
AAAACCGGCCAATTCCTGCCGACCGATCCGGCTGCGCGCTATGCGACGCTCGAATGGCTGATGTTCCAGATGGGCGGCGT
CGGCCCGATGCTCGGCCAGACGCATCACTTCCGCATCTATGCGCCGGAAAAGATCGAGTACGCGATCAACCGCTACACGA
ACGAGGCGAAGCGACTGTACAACGTGATGGACAAGCGCCTCGGCGAATCCGAGTATCTGGCCGGCGACGCGTACACGATC
GCGGACATCGCGACGTTCCCGTGGACGCGTTCGTGGCAGAACCAGGGCATCGAGCTCGACGCATTCCCGAACGTCAAGCG
CTGGCACGAAGCGATCGCCGCACGCCCTGCCGTGCAGCGCGGCGTCGAGGTGCTCGCGTCGCTGCGCAAGGCGCTGCAGG
ACGACAAGGCGCGCGAGGTGCTGTTCGGCGCGACGCAGTACGCGAAGCGCTGA

Upstream 100 bases:

>100_bases
AGGCAAGCCCTGCAGCGCGCATCGATCCGTGCTGTAATGACCGCCTGCCGCGGCGCGCGCGCCGCTTCCGCTTCCGTTGA
TGAACCGAAGGAGTTTGACG

Downstream 100 bases:

>100_bases
GCGGCACGCCGCCGCACGGCTGGGCAGCGGCCGTGCGGCGTCAGCACGTCAGAAGTAGTGCAGCGTGATGAATTCCGCCG
CACAGACCGGGAGCGGTCCG

Product: glutathione S-transferase domain-containing protein

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MIDVYSWATPNGHKVHIMLEETGLAYRAHPIDIGAGDQFNAEFLKISPNNKIPAIVDDEGPGGQPISLFESGAILIYLAE
KTGQFLPTDPAARYATLEWLMFQMGGVGPMLGQTHHFRIYAPEKIEYAINRYTNEAKRLYNVMDKRLGESEYLAGDAYTI
ADIATFPWTRSWQNQGIELDAFPNVKRWHEAIAARPAVQRGVEVLASLRKALQDDKAREVLFGATQYAKR

Sequences:

>Translated_230_residues
MIDVYSWATPNGHKVHIMLEETGLAYRAHPIDIGAGDQFNAEFLKISPNNKIPAIVDDEGPGGQPISLFESGAILIYLAE
KTGQFLPTDPAARYATLEWLMFQMGGVGPMLGQTHHFRIYAPEKIEYAINRYTNEAKRLYNVMDKRLGESEYLAGDAYTI
ADIATFPWTRSWQNQGIELDAFPNVKRWHEAIAARPAVQRGVEVLASLRKALQDDKAREVLFGATQYAKR
>Mature_230_residues
MIDVYSWATPNGHKVHIMLEETGLAYRAHPIDIGAGDQFNAEFLKISPNNKIPAIVDDEGPGGQPISLFESGAILIYLAE
KTGQFLPTDPAARYATLEWLMFQMGGVGPMLGQTHHFRIYAPEKIEYAINRYTNEAKRLYNVMDKRLGESEYLAGDAYTI
ADIATFPWTRSWQNQGIELDAFPNVKRWHEAIAARPAVQRGVEVLASLRKALQDDKAREVLFGATQYAKR

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=218, Percent_Identity=55.045871559633, Blast_Score=239, Evalue=1e-64,
Organism=Escherichia coli, GI87082195, Length=214, Percent_Identity=50, Blast_Score=183, Evalue=6e-48,
Organism=Escherichia coli, GI87081789, Length=187, Percent_Identity=29.9465240641711, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324100, Length=234, Percent_Identity=33.3333333333333, Blast_Score=123, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17933730, Length=222, Percent_Identity=27.4774774774775, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI17864598, Length=204, Percent_Identity=28.921568627451, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17864592, Length=223, Percent_Identity=29.5964125560538, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28571670, Length=173, Percent_Identity=27.7456647398844, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI85725204, Length=202, Percent_Identity=28.7128712871287, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17737923, Length=202, Percent_Identity=28.7128712871287, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17864594, Length=192, Percent_Identity=28.125, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45549270, Length=200, Percent_Identity=27, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17864596, Length=219, Percent_Identity=23.7442922374429, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI19922160, Length=189, Percent_Identity=26.984126984127, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24646251, Length=173, Percent_Identity=27.1676300578035, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 25828; Mature: 25828

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDVYSWATPNGHKVHIMLEETGLAYRAHPIDIGAGDQFNAEFLKISPNNKIPAIVDDEG
CCCEEECCCCCCCEEEEEEECCCCEEEECEEECCCCCCCCCEEEEECCCCCCCEEECCCC
PGGQPISLFESGAILIYLAEKTGQFLPTDPAARYATLEWLMFQMGGVGPMLGQTHHFRIY
CCCCCEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
APEKIEYAINRYTNEAKRLYNVMDKRLGESEYLAGDAYTIADIATFPWTRSWQNQGIELD
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEEHHCCCCCCCCHHHCCEEEE
AFPNVKRWHEAIAARPAVQRGVEVLASLRKALQDDKAREVLFGATQYAKR
CCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIDVYSWATPNGHKVHIMLEETGLAYRAHPIDIGAGDQFNAEFLKISPNNKIPAIVDDEG
CCCEEECCCCCCCEEEEEEECCCCEEEECEEECCCCCCCCCEEEEECCCCCCCEEECCCC
PGGQPISLFESGAILIYLAEKTGQFLPTDPAARYATLEWLMFQMGGVGPMLGQTHHFRIY
CCCCCEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
APEKIEYAINRYTNEAKRLYNVMDKRLGESEYLAGDAYTIADIATFPWTRSWQNQGIELD
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEEHHCCCCCCCCHHHCCEEEE
AFPNVKRWHEAIAARPAVQRGVEVLASLRKALQDDKAREVLFGATQYAKR
CCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]