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The map label for this gene is nuoB [H]

Identifier: 161524205

GI number: 161524205

Start: 1125084

End: 1125563

Strand: Direct

Name: nuoB [H]

Synonym: Bmul_1029

Alternate gene names: 161524205

Gene position: 1125084-1125563 (Clockwise)

Preceding gene: 161524204

Following gene: 161524206

Centisome position: 32.63

GC content: 65.42

Gene sequence:

>480_bases
ATGAGTATCGAAGGGGTCTTGAAGGAAGGGTTTGTCACCACCACGGCTGACAAGCTGATCAACTGGACGCGTACCGGCTC
GCTGTGGCCGATGACGTTCGGGCTCGCGTGTTGCGCCGTCGAGATGATGCATGCGGGCGCGGCCCGTTACGATCTGGACC
GGTTCGGCGTCGTGTTTCGTCCGAGCCCGCGTCAGTCCGACGTGATGATCGTCGCCGGCACGCTGTGCAACAAGATGGCA
CCCGCGCTGCGCCGCGTGTACGACCAGATGGCCGAGCCGCGCTGGGTGATCTCGATGGGGTCGTGCGCGAACGGCGGCGG
CTACTACCACTACTCGTACTCGGTGGTTCGCGGCTGCGACCGGATCGTGCCGGTCGACGTCTACGTGCCGGGCTGCCCGC
CGACGGCCGAGGCGCTGGTCTACGGCGTGATCCAGCTTCAGGCGAAGATCCGCCGCACCAACACCATCGCCCGTCAATAA

Upstream 100 bases:

>100_bases
CCTATATCTGGAAGAAAGGCGGCCTCGACTGGGAGTGATGGGTTAATCGCCGGTTTGCATGGGTGGCCGCGCCCGGTCGC
CCGTCTGGAGTGGAAAGCAA

Downstream 100 bases:

>100_bases
GCCCCGAGCGTCCCCCAATATGGCAAGCAAAATCGAGACCCTCAAGGCCAACCTCGAAGCCGCGCTCGGCGCGCGCGTGG
TGAGCCTTACCGAAGCGATC

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MSIEGVLKEGFVTTTADKLINWTRTGSLWPMTFGLACCAVEMMHAGAARYDLDRFGVVFRPSPRQSDVMIVAGTLCNKMA
PALRRVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALVYGVIQLQAKIRRTNTIARQ

Sequences:

>Translated_159_residues
MSIEGVLKEGFVTTTADKLINWTRTGSLWPMTFGLACCAVEMMHAGAARYDLDRFGVVFRPSPRQSDVMIVAGTLCNKMA
PALRRVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALVYGVIQLQAKIRRTNTIARQ
>Mature_158_residues
SIEGVLKEGFVTTTADKLINWTRTGSLWPMTFGLACCAVEMMHAGAARYDLDRFGVVFRPSPRQSDVMIVAGTLCNKMAP
ALRRVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALVYGVIQLQAKIRRTNTIARQ

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=146, Percent_Identity=78.7671232876712, Blast_Score=256, Evalue=5e-69,
Organism=Escherichia coli, GI1788624, Length=149, Percent_Identity=51.006711409396, Blast_Score=164, Evalue=2e-42,
Organism=Escherichia coli, GI1789074, Length=138, Percent_Identity=35.5072463768116, Blast_Score=97, Evalue=4e-22,
Organism=Escherichia coli, GI1788834, Length=109, Percent_Identity=37.6146788990826, Blast_Score=89, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17509685, Length=145, Percent_Identity=71.0344827586207, Blast_Score=238, Evalue=1e-63,
Organism=Drosophila melanogaster, GI18859983, Length=140, Percent_Identity=80.7142857142857, Blast_Score=258, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24642371, Length=140, Percent_Identity=80.7142857142857, Blast_Score=258, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24651058, Length=140, Percent_Identity=80, Blast_Score=252, Evalue=9e-68,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 17561; Mature: 17429

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
8.8 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
8.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEGVLKEGFVTTTADKLINWTRTGSLWPMTFGLACCAVEMMHAGAARYDLDRFGVVFR
CCCCHHHHCCCEEECHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEC
PSPRQSDVMIVAGTLCNKMAPALRRVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCD
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEHHHHHCCC
RIVPVDVYVPGCPPTAEALVYGVIQLQAKIRRTNTIARQ
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIEGVLKEGFVTTTADKLINWTRTGSLWPMTFGLACCAVEMMHAGAARYDLDRFGVVFR
CCCHHHHCCCEEECHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEC
PSPRQSDVMIVAGTLCNKMAPALRRVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCD
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEHHHHHCCC
RIVPVDVYVPGCPPTAEALVYGVIQLQAKIRRTNTIARQ
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA