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The map label for this gene is pnp [H]

Identifier: 161524200

GI number: 161524200

Start: 1119665

End: 1121812

Strand: Direct

Name: pnp [H]

Synonym: Bmul_1024

Alternate gene names: 161524200

Gene position: 1119665-1121812 (Clockwise)

Preceding gene: 161524199

Following gene: 161524201

Centisome position: 32.47

GC content: 65.83

Gene sequence:

>2148_bases
ATGTCCATGTTCAACAAGGTCGTGAAGGAATTCCAGTGGGGCCAACACAAGGTGCGCCTCGAAACCGGTGAAATCGCGCG
CCAGGCGAGCGGCGCGGTGATCGTCGACGTCGAGGACACCGTCGTGCTCGCGACCGTCGTCGGCGCGAAGTCGGCGAAGC
CGGGCCAGGACTTCTTCCCGCTGACCGTCGACTACATCGAAAAGACCTATTCGGCCGGCAAGATCCCGGGCGGCTTCTTC
CGTCGCGAAGGCCGTCCGTCGGAACACGAAACGCTGACGTCGCGCCTGATCGACCGTCCGCTGCGCCCGCTGTTCCCGGA
AGGCTTCTACAACGAAGTGCAGGTCGTGATCCACGTGCTGTCGGTGAACCCGGAAATCCCGGCCGACATCCCCGCGCTGA
TCGGCGCATCGGCCGCGCTCGCCGTGTCCGGCCTGCCGTTCAACGGCCCGGTCGGCGCTGCGCGCGTCGCGTACGTGAAC
GACCAGTACGTGCTGAACCCGACCCGCGAGCAGCTCAAGTCCTCGCGTCTCGACCTCGTCGTCGCCGGCACCGAGCGTGC
GGTGCTGATGGTCGAATCGGAAGCCGATCAACTGCCGGAAGAGGTGATGCTCGGCGCGGTCGTGTTCGGCCACGAGCAGA
TGCAAACCGCGATCGACGCGATCCACGAGCTCGTGCGCGACGGCGGCAAGCCCGAGTGGGACTGGCAGCCGGCGCCGAAG
GACGAGGCGCTGATCGCCCGCGTGACGGAAATCGCGCAGCCGGAACTGCTCGCCGCGTACCAGATCCGCGACAAGCAGGC
GCGTTCGACGAAGCTGAAGGAAGTGTACGCAGCGACGTCCGCGAAGCTGGAAGAGGATGCGCTGGCGGCCGGCACGGTCG
CGGCCGACAAGGCCACGGTCGGCAACATCCTGTTCGATCTCGAAGCGAAGATCGTCCGCGGCCAGATCCTGAACGGCGAG
CCGCGTATCGACGGCCGCGACACGCGCACCGTGCGTCCGATCGAGATCCGCACGGGCGTGCTGCCGCGCACGCACGGCTC
CGCGCTGTTCACGCGCGGCGAGACGCAGGCGCTCGTCGTCGCGACGCTCGGCACGAAGGGTGACGAGCAGATCATCGACG
CGCTCGAAGGCGAATACCGCGAGCGCTTCATGCTCCACTACAACATGCCGCCGTTCGCGACCGGCGAAACGGGCCGCGTC
GGCTCGCCGAAGCGCCGCGAGATCGGTCACGGCCGCCTCGCGAAGCGCGCGCTCGTCGCGTGCCTGCCGAGCGCCGAAGA
GTTCGGCTACTCGATTCGCGTCGTGTCGGAAATCACCGAGTCGAACGGTTCGTCGTCGATGGCATCGGTGTGCGGCGGCT
GCCTCGCGCTGATGGACGCCGGCGTGCCGATGAAGGCGCACGTCGCGGGCATCGCGATGGGCCTCATCCTCGAAGGCAAC
AAGTTCGCGGTGCTGACCGACATCCTCGGCGACGAAGATCACCTCGGCGACATGGACTTCAAGGTCGCGGGCACCGCGCA
GGGCGTGACCGCGCTGCAGATGGACATCAAGATCCAGGGCATCACGAAGGAAATCATGCAGGTCGCGCTCGCGCAGGCGA
AGGAAGGCCGCATGCACATCCTCGGCAAGATGACGGAAGCGGTGGCCGGCGCGAACACGCAGCTGTCGGAATTCGCACCG
CGCATGATCACGATCAAGATCAACCCGGAAAAGATCCGCGACGTGATCGGCAAGGGCGGCTCGGTGATCCGCGCGCTGAC
CGAAGAAACGGGCACGACGATCGACATCTCCGACGACGGCGTCGTGACGATCGCGAGCACGAGCAGCGAAGGCATGGCCG
AAGCGAAGAAGCGCATCGAGAACATCACGGCCGAGATCGAAGTCGGTCAGGTGTACGAAGGCACGGTGCTGAAGCTGCTC
GATTTCGGCGCGATCGTGAACCTGCTGCCGGGCAAGGACGGCCTGCTGCACATCTCGGAAATCGTCAACGAGCGCGTGAA
GGACATCAACGACTACCTGAAGGAAGGTCAGCAGGTGAAGGTCAAGGTGATCCAGACGGACGAGAAGGGTCGCGTGCGTC
TGTCGGCGAAGGCGCTGCTGAACGAAGCAGCGGCTGCGGCGCAGTCCGATACGCCGCCGCAGCAGTAA

Upstream 100 bases:

>100_bases
GGCATAAAAAACACACCTTGCTCCGGGTGATTCGGTCGGGACGCCGCCGCGCGGGATGACCGGCGCGGCGAACGAAATGC
ATGAATACAAAGGAGCAACC

Downstream 100 bases:

>100_bases
GCGGAACGGCGAACGAACGGCCGGCTGCGCGAAAGCGGGCCGGCCGTTTTTTCTGTAGCATCGTTGTGCGGCGCAGCAGC
GCCGGCCATGCGTCCGCGGG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 715; Mature: 714

Protein sequence:

>715_residues
MSMFNKVVKEFQWGQHKVRLETGEIARQASGAVIVDVEDTVVLATVVGAKSAKPGQDFFPLTVDYIEKTYSAGKIPGGFF
RREGRPSEHETLTSRLIDRPLRPLFPEGFYNEVQVVIHVLSVNPEIPADIPALIGASAALAVSGLPFNGPVGAARVAYVN
DQYVLNPTREQLKSSRLDLVVAGTERAVLMVESEADQLPEEVMLGAVVFGHEQMQTAIDAIHELVRDGGKPEWDWQPAPK
DEALIARVTEIAQPELLAAYQIRDKQARSTKLKEVYAATSAKLEEDALAAGTVAADKATVGNILFDLEAKIVRGQILNGE
PRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVVATLGTKGDEQIIDALEGEYRERFMLHYNMPPFATGETGRV
GSPKRREIGHGRLAKRALVACLPSAEEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGN
KFAVLTDILGDEDHLGDMDFKVAGTAQGVTALQMDIKIQGITKEIMQVALAQAKEGRMHILGKMTEAVAGANTQLSEFAP
RMITIKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDGVVTIASTSSEGMAEAKKRIENITAEIEVGQVYEGTVLKLL
DFGAIVNLLPGKDGLLHISEIVNERVKDINDYLKEGQQVKVKVIQTDEKGRVRLSAKALLNEAAAAAQSDTPPQQ

Sequences:

>Translated_715_residues
MSMFNKVVKEFQWGQHKVRLETGEIARQASGAVIVDVEDTVVLATVVGAKSAKPGQDFFPLTVDYIEKTYSAGKIPGGFF
RREGRPSEHETLTSRLIDRPLRPLFPEGFYNEVQVVIHVLSVNPEIPADIPALIGASAALAVSGLPFNGPVGAARVAYVN
DQYVLNPTREQLKSSRLDLVVAGTERAVLMVESEADQLPEEVMLGAVVFGHEQMQTAIDAIHELVRDGGKPEWDWQPAPK
DEALIARVTEIAQPELLAAYQIRDKQARSTKLKEVYAATSAKLEEDALAAGTVAADKATVGNILFDLEAKIVRGQILNGE
PRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVVATLGTKGDEQIIDALEGEYRERFMLHYNMPPFATGETGRV
GSPKRREIGHGRLAKRALVACLPSAEEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGN
KFAVLTDILGDEDHLGDMDFKVAGTAQGVTALQMDIKIQGITKEIMQVALAQAKEGRMHILGKMTEAVAGANTQLSEFAP
RMITIKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDGVVTIASTSSEGMAEAKKRIENITAEIEVGQVYEGTVLKLL
DFGAIVNLLPGKDGLLHISEIVNERVKDINDYLKEGQQVKVKVIQTDEKGRVRLSAKALLNEAAAAAQSDTPPQQ
>Mature_714_residues
SMFNKVVKEFQWGQHKVRLETGEIARQASGAVIVDVEDTVVLATVVGAKSAKPGQDFFPLTVDYIEKTYSAGKIPGGFFR
REGRPSEHETLTSRLIDRPLRPLFPEGFYNEVQVVIHVLSVNPEIPADIPALIGASAALAVSGLPFNGPVGAARVAYVND
QYVLNPTREQLKSSRLDLVVAGTERAVLMVESEADQLPEEVMLGAVVFGHEQMQTAIDAIHELVRDGGKPEWDWQPAPKD
EALIARVTEIAQPELLAAYQIRDKQARSTKLKEVYAATSAKLEEDALAAGTVAADKATVGNILFDLEAKIVRGQILNGEP
RIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVVATLGTKGDEQIIDALEGEYRERFMLHYNMPPFATGETGRVG
SPKRREIGHGRLAKRALVACLPSAEEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGNK
FAVLTDILGDEDHLGDMDFKVAGTAQGVTALQMDIKIQGITKEIMQVALAQAKEGRMHILGKMTEAVAGANTQLSEFAPR
MITIKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDGVVTIASTSSEGMAEAKKRIENITAEIEVGQVYEGTVLKLLD
FGAIVNLLPGKDGLLHISEIVNERVKDINDYLKEGQQVKVKVIQTDEKGRVRLSAKALLNEAAAAAQSDTPPQQ

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=724, Percent_Identity=37.292817679558, Blast_Score=443, Evalue=1e-124,
Organism=Escherichia coli, GI145693187, Length=697, Percent_Identity=64.7058823529412, Blast_Score=925, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=710, Percent_Identity=32.5352112676056, Blast_Score=325, Evalue=6e-89,
Organism=Drosophila melanogaster, GI281362905, Length=715, Percent_Identity=35.9440559440559, Blast_Score=413, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24651641, Length=715, Percent_Identity=35.9440559440559, Blast_Score=413, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24651643, Length=715, Percent_Identity=35.9440559440559, Blast_Score=413, Evalue=1e-115,
Organism=Drosophila melanogaster, GI161079377, Length=666, Percent_Identity=35.8858858858859, Blast_Score=379, Evalue=1e-105,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 77132; Mature: 77001

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMFNKVVKEFQWGQHKVRLETGEIARQASGAVIVDVEDTVVLATVVGAKSAKPGQDFFP
CCHHHHHHHHHHCCCEEEEEEHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCCCCCCC
LTVDYIEKTYSAGKIPGGFFRREGRPSEHETLTSRLIDRPLRPLFPEGFYNEVQVVIHVL
CCHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEE
SVNPEIPADIPALIGASAALAVSGLPFNGPVGAARVAYVNDQYVLNPTREQLKSSRLDLV
ECCCCCCCCCHHHHCCCHHHEEECCCCCCCCCCEEEEEECCCEEECCHHHHHHCCCCEEE
VAGTERAVLMVESEADQLPEEVMLGAVVFGHEQMQTAIDAIHELVRDGGKPEWDWQPAPK
EECCCEEEEEEECCHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
DEALIARVTEIAQPELLAAYQIRDKQARSTKLKEVYAATSAKLEEDALAAGTVAADKATV
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHH
GNILFDLEAKIVRGQILNGEPRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVV
HHEEEEEHHEEEECEEECCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEECCCCCEEEE
ATLGTKGDEQIIDALEGEYRERFMLHYNMPPFATGETGRVGSPKRREIGHGRLAKRALVA
EEECCCCHHHHHHHHCCHHHEEEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHH
CLPSAEEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGN
HCCCHHHCCCEEEEEEHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHEEEEEEEECC
KFAVLTDILGDEDHLGDMDFKVAGTAQGVTALQMDIKIQGITKEIMQVALAQAKEGRMHI
CEEEEEECCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHCCCCCHH
LGKMTEAVAGANTQLSEFAPRMITIKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDG
HHHHHHHHCCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCC
VVTIASTSSEGMAEAKKRIENITAEIEVGQVYEGTVLKLLDFGAIVNLLPGKDGLLHISE
EEEEECCCCCHHHHHHHHHHHCEEEEEECCEECCHHHHHHHHHHHHHHCCCCCCCCHHHH
IVNERVKDINDYLKEGQQVKVKVIQTDEKGRVRLSAKALLNEAAAAAQSDTPPQQ
HHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SMFNKVVKEFQWGQHKVRLETGEIARQASGAVIVDVEDTVVLATVVGAKSAKPGQDFFP
CHHHHHHHHHHCCCEEEEEEHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCCCCCCC
LTVDYIEKTYSAGKIPGGFFRREGRPSEHETLTSRLIDRPLRPLFPEGFYNEVQVVIHVL
CCHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEE
SVNPEIPADIPALIGASAALAVSGLPFNGPVGAARVAYVNDQYVLNPTREQLKSSRLDLV
ECCCCCCCCCHHHHCCCHHHEEECCCCCCCCCCEEEEEECCCEEECCHHHHHHCCCCEEE
VAGTERAVLMVESEADQLPEEVMLGAVVFGHEQMQTAIDAIHELVRDGGKPEWDWQPAPK
EECCCEEEEEEECCHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
DEALIARVTEIAQPELLAAYQIRDKQARSTKLKEVYAATSAKLEEDALAAGTVAADKATV
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHH
GNILFDLEAKIVRGQILNGEPRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVV
HHEEEEEHHEEEECEEECCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEECCCCCEEEE
ATLGTKGDEQIIDALEGEYRERFMLHYNMPPFATGETGRVGSPKRREIGHGRLAKRALVA
EEECCCCHHHHHHHHCCHHHEEEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHH
CLPSAEEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGN
HCCCHHHCCCEEEEEEHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHEEEEEEEECC
KFAVLTDILGDEDHLGDMDFKVAGTAQGVTALQMDIKIQGITKEIMQVALAQAKEGRMHI
CEEEEEECCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHCCCCCHH
LGKMTEAVAGANTQLSEFAPRMITIKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDG
HHHHHHHHCCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCC
VVTIASTSSEGMAEAKKRIENITAEIEVGQVYEGTVLKLLDFGAIVNLLPGKDGLLHISE
EEEEECCCCCHHHHHHHHHHHCEEEEEECCEECCHHHHHHHHHHHHHHCCCCCCCCHHHH
IVNERVKDINDYLKEGQQVKVKVIQTDEKGRVRLSAKALLNEAAAAAQSDTPPQQ
HHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA