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The map label for this gene is parE [H]

Identifier: 161524095

GI number: 161524095

Start: 1004096

End: 1006078

Strand: Direct

Name: parE [H]

Synonym: Bmul_0919

Alternate gene names: 161524095

Gene position: 1004096-1006078 (Clockwise)

Preceding gene: 161524094

Following gene: 161524096

Centisome position: 29.12

GC content: 66.26

Gene sequence:

>1983_bases
ATGTCAACGAAGAAGCCCAGCGCGGCCTATAGCGAAGCATCGATCAAGGTGCTCAAGGGTCTCGAGCCGGTCAAGCAGCG
GCCCGGCATGTATACCCGCACCGAGAATCCGCTGCACATCATCCAGGAAGTCATCGACAACGCGTCCGACGAGGCGCTCG
GCGGCTACGGCAAGCAGATCACGGTCACGCTGCACGCGGATCAGTCGGTGTCCGTCGAGGACGACGGCCGCGGCATTCCG
TTCGGCCTGCATCCCGACGAAGGCGTGCCGGTCGTCGAAATCGTGTTCACGCGCCTGCATGCAGGCGGCAAGTTCGACAA
GGCGGCGGGCGGCGCATACACGTTCTCGGGCGGCTTGCATGGCGTCGGCGTGTCGGTGACGAACGCGCTCGCGACGCGGC
TCGACGTGACCGTCTGGCGCGACGGCAAGATCGCGGAGCTCGGTTTTGCCGACGGCGACGTCGTCAAGCCGCTGTCCACG
CAGCCGGCCGGCCGCGGCGAGAAGAAGTCGGGCACGCGCGTGCGCGTATGGCCGAATCCGAAATACTTCGACTCGGCGAA
CCTGCCGCTCGGCGAACTGCAGCGCCTGCTGCGCTCGAAGGCCGTGCTGCTGCCGGGCGTCGAGGTCGTGCTCGTCAACG
AGAAGAGCGGCGAGCGCCAGAGCTGGAAATACGAGGACGGCCTGCGCGGCTATCTGCTCGACGAGATGAACGGCAGCGAG
CTGCTGATTCCGCTGTTCGAGGGCGAACGCTTCGCCGATTCGCGTTCGGGCGACGACACGTTCGCCGAAGGCGAGGGCGC
GTCGTGGGTCGTCGCCTGGAGCGAGGAAGGCTCGCTCGTGCGCGAGTCGTACGTGAACCTGATCCCGACGCCGGCCGGCG
GCACGCATGAATCGGGCCTGCGCGACGGTCTCTATCAGGCGGTGAAGAGCTTCGTCGAACTGCACAACCTGCAGCCGAAG
GGCGTGAAGCTGCTCGCCGAAGACGTGTTCGCGCGCGTGTCGTTCGTGCTGTCCGCGAAGGTGCTCGATCCGCAGTTCCA
AGGGCAGATCAAGGAACGGCTGAACAGCCGCGACGCGGTGAAGCTCGTGTCGTCGTTCTCGCGTCCGGCGCTCGAGCTGT
GGCTGAACCAGCACGTCGAGCACGGCAAGAAGCTCGCGGAGCTCGTGATCAAGCAGGCGCAGGCGCGCACGCGCGCGGGC
CAGAAGGTCGAAAAGCGCAAGAGCTCGGGCGTCGCGGTGCTGCCGGGCAAGCTGACCGACTGCGAGGCCGAGGACATCGC
GCGCAACGAGCTGTTCCTCGTCGAAGGCGACTCGGCGGGCGGCTCCGCGAAGATGGGCCGCGACAAGGAATACCAGGCGA
TCCTGCCGCTGCGCGGCAAGGTACTGAACACGTGGGAAACCGAGCGCGACCGTCTGTTCGCGAACAACGAGGTGCACGAC
ATTTCGGTCGCGATTGGCGTCGATCCGCACGGCCCGGACGACAGCGTCGATCTGTCGAACCTGCGCTACGGCAAGATCTG
CATCCTGTCGGATGCGGACGTCGACGGCTCGCACATCCAGGTGCTGCTGCTCACGCTGTTCTTCAAGCATTTCCCGCAGC
TGATCGAGCGCGGCCACGTGTACGTCGCGCGGCCGCCGCTGTTCCGCGTCGACGCGCCGGCGCGCGGCAAGAAGCCCGCG
CAGAAGCTCTACGCGCTCGACGAAGGCGAACTGGAAGCGATCCTCGACAAGCTGCGCAAGGACGGCGTACGCGAAACGCA
ATGGAGCATCAGCCGCTTCAAGGGTCTCGGCGAAATGAGCGCGGAGCAGCTGTGGGACACGACGATGAACCCCGACACGC
GCCGCCTGATGCCGGTGAAGCTCGGCGAGCTCGACTACGAGACGACCGTCGCGCGGATGACGATGCTGATGGGCAAGGGC
GAGGCGGCCGCACGGCGCGGCTGGCTCGAGGAAAAGGGCAACGACGTCGAAGCGGATATCTAA

Upstream 100 bases:

>100_bases
TTGTTTCGATTCGCTTTTTTACGGAACTCAACGTTTTGTCCACGACGTTATCCACACGAGGTGTGGACAACGCCCGGACG
CATGACGATATTCAGCGCCT

Downstream 100 bases:

>100_bases
GCGCCCCGAGCGCGGCTGCGCATGACGCGCGGCGCGCCCTCACGCCACTTACGAATACGGAATTCAGATGGACGACAACA
CCTCCGATCTCTTTGCCGGG

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 660; Mature: 659

Protein sequence:

>660_residues
MSTKKPSAAYSEASIKVLKGLEPVKQRPGMYTRTENPLHIIQEVIDNASDEALGGYGKQITVTLHADQSVSVEDDGRGIP
FGLHPDEGVPVVEIVFTRLHAGGKFDKAAGGAYTFSGGLHGVGVSVTNALATRLDVTVWRDGKIAELGFADGDVVKPLST
QPAGRGEKKSGTRVRVWPNPKYFDSANLPLGELQRLLRSKAVLLPGVEVVLVNEKSGERQSWKYEDGLRGYLLDEMNGSE
LLIPLFEGERFADSRSGDDTFAEGEGASWVVAWSEEGSLVRESYVNLIPTPAGGTHESGLRDGLYQAVKSFVELHNLQPK
GVKLLAEDVFARVSFVLSAKVLDPQFQGQIKERLNSRDAVKLVSSFSRPALELWLNQHVEHGKKLAELVIKQAQARTRAG
QKVEKRKSSGVAVLPGKLTDCEAEDIARNELFLVEGDSAGGSAKMGRDKEYQAILPLRGKVLNTWETERDRLFANNEVHD
ISVAIGVDPHGPDDSVDLSNLRYGKICILSDADVDGSHIQVLLLTLFFKHFPQLIERGHVYVARPPLFRVDAPARGKKPA
QKLYALDEGELEAILDKLRKDGVRETQWSISRFKGLGEMSAEQLWDTTMNPDTRRLMPVKLGELDYETTVARMTMLMGKG
EAAARRGWLEEKGNDVEADI

Sequences:

>Translated_660_residues
MSTKKPSAAYSEASIKVLKGLEPVKQRPGMYTRTENPLHIIQEVIDNASDEALGGYGKQITVTLHADQSVSVEDDGRGIP
FGLHPDEGVPVVEIVFTRLHAGGKFDKAAGGAYTFSGGLHGVGVSVTNALATRLDVTVWRDGKIAELGFADGDVVKPLST
QPAGRGEKKSGTRVRVWPNPKYFDSANLPLGELQRLLRSKAVLLPGVEVVLVNEKSGERQSWKYEDGLRGYLLDEMNGSE
LLIPLFEGERFADSRSGDDTFAEGEGASWVVAWSEEGSLVRESYVNLIPTPAGGTHESGLRDGLYQAVKSFVELHNLQPK
GVKLLAEDVFARVSFVLSAKVLDPQFQGQIKERLNSRDAVKLVSSFSRPALELWLNQHVEHGKKLAELVIKQAQARTRAG
QKVEKRKSSGVAVLPGKLTDCEAEDIARNELFLVEGDSAGGSAKMGRDKEYQAILPLRGKVLNTWETERDRLFANNEVHD
ISVAIGVDPHGPDDSVDLSNLRYGKICILSDADVDGSHIQVLLLTLFFKHFPQLIERGHVYVARPPLFRVDAPARGKKPA
QKLYALDEGELEAILDKLRKDGVRETQWSISRFKGLGEMSAEQLWDTTMNPDTRRLMPVKLGELDYETTVARMTMLMGKG
EAAARRGWLEEKGNDVEADI
>Mature_659_residues
STKKPSAAYSEASIKVLKGLEPVKQRPGMYTRTENPLHIIQEVIDNASDEALGGYGKQITVTLHADQSVSVEDDGRGIPF
GLHPDEGVPVVEIVFTRLHAGGKFDKAAGGAYTFSGGLHGVGVSVTNALATRLDVTVWRDGKIAELGFADGDVVKPLSTQ
PAGRGEKKSGTRVRVWPNPKYFDSANLPLGELQRLLRSKAVLLPGVEVVLVNEKSGERQSWKYEDGLRGYLLDEMNGSEL
LIPLFEGERFADSRSGDDTFAEGEGASWVVAWSEEGSLVRESYVNLIPTPAGGTHESGLRDGLYQAVKSFVELHNLQPKG
VKLLAEDVFARVSFVLSAKVLDPQFQGQIKERLNSRDAVKLVSSFSRPALELWLNQHVEHGKKLAELVIKQAQARTRAGQ
KVEKRKSSGVAVLPGKLTDCEAEDIARNELFLVEGDSAGGSAKMGRDKEYQAILPLRGKVLNTWETERDRLFANNEVHDI
SVAIGVDPHGPDDSVDLSNLRYGKICILSDADVDGSHIQVLLLTLFFKHFPQLIERGHVYVARPPLFRVDAPARGKKPAQ
KLYALDEGELEAILDKLRKDGVRETQWSISRFKGLGEMSAEQLWDTTMNPDTRRLMPVKLGELDYETTVARMTMLMGKGE
AAARRGWLEEKGNDVEADI

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=647, Percent_Identity=23.9567233384853, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI19913406, Length=652, Percent_Identity=23.7730061349693, Blast_Score=116, Evalue=6e-26,
Organism=Escherichia coli, GI1789408, Length=656, Percent_Identity=50.609756097561, Blast_Score=644, Evalue=0.0,
Organism=Escherichia coli, GI48994957, Length=659, Percent_Identity=36.7223065250379, Blast_Score=365, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI212645845, Length=623, Percent_Identity=23.916532905297, Blast_Score=105, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17535065, Length=643, Percent_Identity=23.7947122861586, Blast_Score=105, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI212645657, Length=213, Percent_Identity=29.1079812206573, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=656, Percent_Identity=25, Blast_Score=130, Evalue=5e-31,
Organism=Drosophila melanogaster, GI17136538, Length=661, Percent_Identity=24.9621785173979, Blast_Score=121, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.-

Molecular weight: Translated: 72550; Mature: 72419

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKKPSAAYSEASIKVLKGLEPVKQRPGMYTRTENPLHIIQEVIDNASDEALGGYGKQI
CCCCCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEE
TVTLHADQSVSVEDDGRGIPFGLHPDEGVPVVEIVFTRLHAGGKFDKAAGGAYTFSGGLH
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEECCCCC
GVGVSVTNALATRLDVTVWRDGKIAELGFADGDVVKPLSTQPAGRGEKKSGTRVRVWPNP
CCCHHHHHHHHHHEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCC
KYFDSANLPLGELQRLLRSKAVLLPGVEVVLVNEKSGERQSWKYEDGLRGYLLDEMNGSE
CCCCCCCCCHHHHHHHHHHCCEECCCEEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCE
LLIPLFEGERFADSRSGDDTFAEGEGASWVVAWSEEGSLVRESYVNLIPTPAGGTHESGL
EEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHEECCCCCCCCCHHH
RDGLYQAVKSFVELHNLQPKGVKLLAEDVFARVSFVLSAKVLDPQFQGQIKERLNSRDAV
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHH
KLVSSFSRPALELWLNQHVEHGKKLAELVIKQAQARTRAGQKVEKRKSSGVAVLPGKLTD
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
CEAEDIARNELFLVEGDSAGGSAKMGRDKEYQAILPLRGKVLNTWETERDRLFANNEVHD
CCHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCEEECCCCCCHHHEECCCCEEE
ISVAIGVDPHGPDDSVDLSNLRYGKICILSDADVDGSHIQVLLLTLFFKHFPQLIERGHV
EEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCE
YVARPPLFRVDAPARGKKPAQKLYALDEGELEAILDKLRKDGVRETQWSISRFKGLGEMS
EEECCCEEEECCCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHH
AEQLWDTTMNPDTRRLMPVKLGELDYETTVARMTMLMGKGEAAARRGWLEEKGNDVEADI
HHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHCCCHHHCCCCCCCCC
>Mature Secondary Structure 
STKKPSAAYSEASIKVLKGLEPVKQRPGMYTRTENPLHIIQEVIDNASDEALGGYGKQI
CCCCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEE
TVTLHADQSVSVEDDGRGIPFGLHPDEGVPVVEIVFTRLHAGGKFDKAAGGAYTFSGGLH
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEECCCCC
GVGVSVTNALATRLDVTVWRDGKIAELGFADGDVVKPLSTQPAGRGEKKSGTRVRVWPNP
CCCHHHHHHHHHHEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCC
KYFDSANLPLGELQRLLRSKAVLLPGVEVVLVNEKSGERQSWKYEDGLRGYLLDEMNGSE
CCCCCCCCCHHHHHHHHHHCCEECCCEEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCE
LLIPLFEGERFADSRSGDDTFAEGEGASWVVAWSEEGSLVRESYVNLIPTPAGGTHESGL
EEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHEECCCCCCCCCHHH
RDGLYQAVKSFVELHNLQPKGVKLLAEDVFARVSFVLSAKVLDPQFQGQIKERLNSRDAV
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHH
KLVSSFSRPALELWLNQHVEHGKKLAELVIKQAQARTRAGQKVEKRKSSGVAVLPGKLTD
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
CEAEDIARNELFLVEGDSAGGSAKMGRDKEYQAILPLRGKVLNTWETERDRLFANNEVHD
CCHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCEEECCCCCCHHHEECCCCEEE
ISVAIGVDPHGPDDSVDLSNLRYGKICILSDADVDGSHIQVLLLTLFFKHFPQLIERGHV
EEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCE
YVARPPLFRVDAPARGKKPAQKLYALDEGELEAILDKLRKDGVRETQWSISRFKGLGEMS
EEECCCEEEECCCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHH
AEQLWDTTMNPDTRRLMPVKLGELDYETTVARMTMLMGKGEAAARRGWLEEKGNDVEADI
HHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2170028; 8388096; 9278503; 8227000; 8980775 [H]