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The map label for this gene is flhA [H]

Identifier: 161523873

GI number: 161523873

Start: 762349

End: 763455

Strand: Direct

Name: flhA [H]

Synonym: Bmul_0693

Alternate gene names: 161523873

Gene position: 762349-763455 (Clockwise)

Preceding gene: 161523872

Following gene: 161523874

Centisome position: 22.11

GC content: 64.95

Gene sequence:

>1107_bases
ATGAAGACGAAAGCAGCGATTGCATGGAAGGCGGGCGCGCCGCTGACGATCGAGGAAGTCGATCTCGAAGGCCCGCGTGC
GGGCGAAGTGCTGATCGAGGTGAAGGCGACGGGCATCTGCCACACCGACTACTACACGCTGTCGGGCGCCGATCCGGAAG
GCATCTTCCCGGCGATCCTCGGCCATGAAGGCGCGGGCGTCGTCGTCGACGTCGGTCCGGGCGTCGGCACGGTGCGCAAG
GGCGATCACGTGATTCCGCTCTACACGCCCGAATGCCGCGAGTGCAAGTTCTGCCTGTCGCGCAAGACCAACCTCTGCCA
GAAGATCCGCGCGACGCAGGGCAAGGGCCTGATGCCCGACGCGACCTCGCGGTTCTCGCTCGGCGGCAAGCCGCTGTTCC
ACTACATGGGCACCTCGACGTTCTCGAACTACATCGTCGTGCCCGAGATCGCGGTCGCGAAGGTGCGCGAGGACGCGCCG
TTCGACAAGATCTGCTACATCGGCTGCGGCGTGACGACGGGCGTCGGCGCGGTCGTGTACTCGGCGAAGGTCGAGGCCGG
CGCGAACGTCGTCGTGTTCGGTCTCGGCGGGATCGGGCTGAACGTGATCCAGGGTGCGAAGATGGTCGGCGCGGACAAGA
TCATCGGCGTCGACATCAATCCGAAGCGCGTCGAACTCGCGAAGAAGTTCGGCATGACGCACTTCATCAACCCGAACGAA
GTCGAGAACGTCGTCGACCACATCGTGCAGCTGACCGACGGCGGCGCCGACTACTCGTTCGAATGCGTCGGCAACGTGAA
GCTGATGCGCCAGGCGCTCGAGTGCACGCACAAGGGCTGGGGCCAGTCGTTCATCATCGGCGTGGCCGCGGCCGGCGAGG
AAATCAGCACGCGTCCGTTCCAGCTCGTCACGGGCCGCGAATGGAAGGGCTCCGCGTTCGGCGGCGCGCGCGGCCGCACC
GACGTGCCGAAGATCGTCGACTGGTACATGGAAGGCAGGATCAACATCGACGATCTGATCACGCACACGCTGCCGCTCGA
GCGCATCAACGAAGGCTTCGACCTGATGAAGAAGGGCGAGTCGATCCGCTCGGTCGTGCTGTACTGA

Upstream 100 bases:

>100_bases
TTTCCGCAGGCCGGCGCGCGATGCCGCGCGGCGCGGAATGGAACCGGAGTCGACGCGGCGGCGCGCCGCGCCGGACCGAC
ATTCAACAAGGAGTCACACG

Downstream 100 bases:

>100_bases
CCGACGGAGCGCGATCATGCTCGAACTCGTTTCATCGCATGCGTGCCACGGCGGCGAGCAGCGCTTCTATCGTCACGATT
CGACGACGATCGGCCTGCCG

Product: S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase

Products: NA

Alternate protein names: Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKTKAAIAWKAGAPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGIFPAILGHEGAGVVVDVGPGVGTVRK
GDHVIPLYTPECRECKFCLSRKTNLCQKIRATQGKGLMPDATSRFSLGGKPLFHYMGTSTFSNYIVVPEIAVAKVREDAP
FDKICYIGCGVTTGVGAVVYSAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDINPKRVELAKKFGMTHFINPNE
VENVVDHIVQLTDGGADYSFECVGNVKLMRQALECTHKGWGQSFIIGVAAAGEEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMEGRINIDDLITHTLPLERINEGFDLMKKGESIRSVVLY

Sequences:

>Translated_368_residues
MKTKAAIAWKAGAPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGIFPAILGHEGAGVVVDVGPGVGTVRK
GDHVIPLYTPECRECKFCLSRKTNLCQKIRATQGKGLMPDATSRFSLGGKPLFHYMGTSTFSNYIVVPEIAVAKVREDAP
FDKICYIGCGVTTGVGAVVYSAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDINPKRVELAKKFGMTHFINPNE
VENVVDHIVQLTDGGADYSFECVGNVKLMRQALECTHKGWGQSFIIGVAAAGEEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMEGRINIDDLITHTLPLERINEGFDLMKKGESIRSVVLY
>Mature_368_residues
MKTKAAIAWKAGAPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGIFPAILGHEGAGVVVDVGPGVGTVRK
GDHVIPLYTPECRECKFCLSRKTNLCQKIRATQGKGLMPDATSRFSLGGKPLFHYMGTSTFSNYIVVPEIAVAKVREDAP
FDKICYIGCGVTTGVGAVVYSAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDINPKRVELAKKFGMTHFINPNE
VENVVDHIVQLTDGGADYSFECVGNVKLMRQALECTHKGWGQSFIIGVAAAGEEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMEGRINIDDLITHTLPLERINEGFDLMKKGESIRSVVLY

Specific function: Essential for the oxidation of formaldehyde produced during methylotrophic growth [H]

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=367, Percent_Identity=60.4904632152589, Blast_Score=454, Evalue=1e-128,
Organism=Homo sapiens, GI71565152, Length=374, Percent_Identity=47.0588235294118, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI34577061, Length=369, Percent_Identity=45.7994579945799, Blast_Score=350, Evalue=1e-96,
Organism=Homo sapiens, GI4501933, Length=369, Percent_Identity=45.5284552845528, Blast_Score=346, Evalue=2e-95,
Organism=Homo sapiens, GI4501929, Length=369, Percent_Identity=45.2574525745258, Blast_Score=341, Evalue=7e-94,
Organism=Homo sapiens, GI262073058, Length=369, Percent_Identity=43.6314363143631, Blast_Score=335, Evalue=4e-92,
Organism=Homo sapiens, GI71743840, Length=369, Percent_Identity=43.6314363143631, Blast_Score=335, Evalue=4e-92,
Organism=Homo sapiens, GI156523966, Length=368, Percent_Identity=43.4782608695652, Blast_Score=333, Evalue=2e-91,
Organism=Homo sapiens, GI4501939, Length=361, Percent_Identity=43.4903047091413, Blast_Score=325, Evalue=3e-89,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=58.8075880758808, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI87082125, Length=379, Percent_Identity=26.9129287598945, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1788073, Length=359, Percent_Identity=27.0194986072423, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786825, Length=380, Percent_Identity=25.2631578947368, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790045, Length=376, Percent_Identity=27.1276595744681, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787863, Length=347, Percent_Identity=26.2247838616715, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI226510992, Length=372, Percent_Identity=23.9247311827957, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI87081918, Length=342, Percent_Identity=28.0701754385965, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788075, Length=382, Percent_Identity=26.9633507853403, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788407, Length=229, Percent_Identity=27.9475982532751, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790718, Length=326, Percent_Identity=24.5398773006135, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786518, Length=367, Percent_Identity=24.7956403269755, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25146526, Length=373, Percent_Identity=54.6916890080429, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71997431, Length=375, Percent_Identity=54.4, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17565904, Length=337, Percent_Identity=55.4896142433234, Blast_Score=398, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17562584, Length=367, Percent_Identity=28.3378746594005, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17562876, Length=363, Percent_Identity=26.4462809917355, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI71988145, Length=371, Percent_Identity=25.8760107816712, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17562582, Length=330, Percent_Identity=25.7575757575758, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17562878, Length=360, Percent_Identity=24.1666666666667, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320033, Length=371, Percent_Identity=53.9083557951482, Blast_Score=430, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6323729, Length=364, Percent_Identity=28.021978021978, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324486, Length=372, Percent_Identity=26.8817204301075, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319621, Length=372, Percent_Identity=27.1505376344086, Blast_Score=96, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6323099, Length=373, Percent_Identity=27.6139410187668, Blast_Score=96, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6322619, Length=363, Percent_Identity=28.3746556473829, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323961, Length=363, Percent_Identity=25.8953168044077, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6319955, Length=363, Percent_Identity=28.3746556473829, Blast_Score=93, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6319257, Length=367, Percent_Identity=26.158038147139, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319258, Length=256, Percent_Identity=28.515625, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI17737895, Length=368, Percent_Identity=60.3260869565217, Blast_Score=477, Evalue=1e-135,
Organism=Drosophila melanogaster, GI17137530, Length=341, Percent_Identity=26.9794721407625, Blast_Score=92, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284 [H]

Molecular weight: Translated: 39562; Mature: 39562

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKAAIAWKAGAPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGIFPAIL
CCCCEEEEEECCCCEEEEEECCCCCCCCEEEEEEEECEEEECCEEEECCCCCCCCCHHHC
GHEGAGVVVDVGPGVGTVRKGDHVIPLYTPECRECKFCLSRKTNLCQKIRATQGKGLMPD
CCCCCEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ATSRFSLGGKPLFHYMGTSTFSNYIVVPEIAVAKVREDAPFDKICYIGCGVTTGVGAVVY
CCCCCCCCCCHHEEHHCCCCCCCEEEECCHHHHHHHCCCCCCCEEEEECCCHHCCCCEEE
SAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDINPKRVELAKKFGMTHFINPNE
EEEECCCCCEEEEECCCCCHHHHCCCEEECCCEEEEECCCCHHHHHHHHHCCEECCCCHH
VENVVDHIVQLTDGGADYSFECVGNVKLMRQALECTHKGWGQSFIIGVAAAGEEISTRPF
HHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCE
QLVTGREWKGSAFGGARGRTDVPKIVDWYMEGRINIDDLITHTLPLERINEGFDLMKKGE
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCHHHHHCCHHHHHCCC
SIRSVVLY
CHHHEECC
>Mature Secondary Structure
MKTKAAIAWKAGAPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGIFPAIL
CCCCEEEEEECCCCEEEEEECCCCCCCCEEEEEEEECEEEECCEEEECCCCCCCCCHHHC
GHEGAGVVVDVGPGVGTVRKGDHVIPLYTPECRECKFCLSRKTNLCQKIRATQGKGLMPD
CCCCCEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ATSRFSLGGKPLFHYMGTSTFSNYIVVPEIAVAKVREDAPFDKICYIGCGVTTGVGAVVY
CCCCCCCCCCHHEEHHCCCCCCCEEEECCHHHHHHHCCCCCCCEEEEECCCHHCCCCEEE
SAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDINPKRVELAKKFGMTHFINPNE
EEEECCCCCEEEEECCCCCHHHHCCCEEECCCEEEEECCCCHHHHHHHHHCCEECCCCHH
VENVVDHIVQLTDGGADYSFECVGNVKLMRQALECTHKGWGQSFIIGVAAAGEEISTRPF
HHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCE
QLVTGREWKGSAFGGARGRTDVPKIVDWYMEGRINIDDLITHTLPLERINEGFDLMKKGE
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCHHHHHCCHHHHHCCC
SIRSVVLY
CHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7798140 [H]