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The map label for this gene is yaiM [C]

Identifier: 161523874

GI number: 161523874

Start: 763472

End: 764320

Strand: Direct

Name: yaiM [C]

Synonym: Bmul_0694

Alternate gene names: 161523874

Gene position: 763472-764320 (Clockwise)

Preceding gene: 161523873

Following gene: 161523879

Centisome position: 22.14

GC content: 68.67

Gene sequence:

>849_bases
ATGCTCGAACTCGTTTCATCGCATGCGTGCCACGGCGGCGAGCAGCGCTTCTATCGTCACGATTCGACGACGATCGGCCT
GCCGATGAAGTTCTCGGTCTATCTGCCGCCGCAGGCTGCGCACGGCCCTGTGCCGGCGCTGTTCTATCTCGCCGGGCTCA
CCTGCACGGAAGAGACGTTCGCGATCAAGGCCGGCGCGCAGCAGTATGCGGCGCAGCACGGGCTCGCGCTCGTCGCGCCC
GACACGAGCCCGCGCGGTGCGGGCGTGCCGGGCGAAACCGATGCGTGGGATTTCGGCGTCGGCGCGGGCTTCTACTTGGA
TGCGACCGAGGCGCCGTGGTCCACGCATTACCGGATGGAGACCTACGTGACGCGCGAGCTGCGCGAGATCGTCACGGCCG
AGCTGCCGATCGACGGCACGCGGCTCGGCATCTTCGGCCACTCGATGGGCGGGCACGGCGCGCTGACGCTCGCGCTGCGT
CATCCGGACCTGTACCGGTCGGTGTCGGCGTTCGCGCCGATCGCGGCGCCGACGCGCTGCCCGTGGGGCGAGAAGGCGTT
CTCCGGCTACCTCGGCGCGGATCGCGAAGCGTGGAAGCAGCACGACGCGAGCGAGCTCGTCGCGCGTGCGGATGCGCCGA
AGTTCGCGGACGGCATCCTCGTCGACCAGGGCCTCGCCGATCAGTTCCTCGCGAACCAGCTGAACCCGGACGTATTCGAA
GCCGCGTGCGCGAAGGCCGGCCAGCCGCTGACGCTGCGCCGCCATCCGGGCTACGACCACGGGTATTACTTCATCTCGAC
GTTCATCGCCGATCACATCGCGCATCATGCGCGTGTGCTGATGCGCTGA

Upstream 100 bases:

>100_bases
CACACGCTGCCGCTCGAGCGCATCAACGAAGGCTTCGACCTGATGAAGAAGGGCGAGTCGATCCGCTCGGTCGTGCTGTA
CTGACCGACGGAGCGCGATC

Downstream 100 bases:

>100_bases
GCCGCCGCGTGTCACGTACGCGGCACGTCGCATCGTGCGACGCGGCTCCGATAAAAAACGCCGGCACTGCCTTCGGGCAT
GCCGGCGTTTTTCGTTCGAT

Product: S-formylglutathione hydrolase

Products: NA

Alternate protein names: FGH [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MLELVSSHACHGGEQRFYRHDSTTIGLPMKFSVYLPPQAAHGPVPALFYLAGLTCTEETFAIKAGAQQYAAQHGLALVAP
DTSPRGAGVPGETDAWDFGVGAGFYLDATEAPWSTHYRMETYVTRELREIVTAELPIDGTRLGIFGHSMGGHGALTLALR
HPDLYRSVSAFAPIAAPTRCPWGEKAFSGYLGADREAWKQHDASELVARADAPKFADGILVDQGLADQFLANQLNPDVFE
AACAKAGQPLTLRRHPGYDHGYYFISTFIADHIAHHARVLMR

Sequences:

>Translated_282_residues
MLELVSSHACHGGEQRFYRHDSTTIGLPMKFSVYLPPQAAHGPVPALFYLAGLTCTEETFAIKAGAQQYAAQHGLALVAP
DTSPRGAGVPGETDAWDFGVGAGFYLDATEAPWSTHYRMETYVTRELREIVTAELPIDGTRLGIFGHSMGGHGALTLALR
HPDLYRSVSAFAPIAAPTRCPWGEKAFSGYLGADREAWKQHDASELVARADAPKFADGILVDQGLADQFLANQLNPDVFE
AACAKAGQPLTLRRHPGYDHGYYFISTFIADHIAHHARVLMR
>Mature_282_residues
MLELVSSHACHGGEQRFYRHDSTTIGLPMKFSVYLPPQAAHGPVPALFYLAGLTCTEETFAIKAGAQQYAAQHGLALVAP
DTSPRGAGVPGETDAWDFGVGAGFYLDATEAPWSTHYRMETYVTRELREIVTAELPIDGTRLGIFGHSMGGHGALTLALR
HPDLYRSVSAFAPIAAPTRCPWGEKAFSGYLGADREAWKQHDASELVARADAPKFADGILVDQGLADQFLANQLNPDVFE
AACAKAGQPLTLRRHPGYDHGYYFISTFIADHIAHHARVLMR

Specific function: Serine hydrolase involved in the detoxification of formaldehyde. Hydrolyzes S-formylglutathione to glutathione and formate [H]

COG id: COG0627

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the esterase D family [H]

Homologues:

Organism=Homo sapiens, GI33413400, Length=280, Percent_Identity=56.0714285714286, Blast_Score=342, Evalue=3e-94,
Organism=Escherichia coli, GI1786551, Length=279, Percent_Identity=48.0286738351254, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1788477, Length=281, Percent_Identity=51.2455516014235, Blast_Score=281, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17510185, Length=281, Percent_Identity=49.4661921708185, Blast_Score=285, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6322393, Length=297, Percent_Identity=37.7104377104377, Blast_Score=196, Evalue=2e-51,
Organism=Drosophila melanogaster, GI45551932, Length=282, Percent_Identity=53.9007092198582, Blast_Score=306, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24648347, Length=282, Percent_Identity=53.9007092198582, Blast_Score=305, Evalue=3e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000801
- InterPro:   IPR014186 [H]

Pfam domain/function: PF00756 Esterase [H]

EC number: =3.1.2.12 [H]

Molecular weight: Translated: 30663; Mature: 30663

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLELVSSHACHGGEQRFYRHDSTTIGLPMKFSVYLPPQAAHGPVPALFYLAGLTCTEETF
CCCCCCCCCCCCCCHHEEECCCCEECCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCE
AIKAGAQQYAAQHGLALVAPDTSPRGAGVPGETDAWDFGVGAGFYLDATEAPWSTHYRME
EEECCHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHH
TYVTRELREIVTAELPIDGTRLGIFGHSMGGHGALTLALRHPDLYRSVSAFAPIAAPTRC
HHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEEECCHHHHHHHHHCCCCCCCCC
PWGEKAFSGYLGADREAWKQHDASELVARADAPKFADGILVDQGLADQFLANQLNPDVFE
CCCHHHHHCCCCCCHHHHHHCCHHHHHHHCCCCCHHCCEEECCCHHHHHHHHCCCHHHHH
AACAKAGQPLTLRRHPGYDHGYYFISTFIADHIAHHARVLMR
HHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLELVSSHACHGGEQRFYRHDSTTIGLPMKFSVYLPPQAAHGPVPALFYLAGLTCTEETF
CCCCCCCCCCCCCCHHEEECCCCEECCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCE
AIKAGAQQYAAQHGLALVAPDTSPRGAGVPGETDAWDFGVGAGFYLDATEAPWSTHYRME
EEECCHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHH
TYVTRELREIVTAELPIDGTRLGIFGHSMGGHGALTLALRHPDLYRSVSAFAPIAAPTRC
HHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEEECCHHHHHHHHHCCCCCCCCC
PWGEKAFSGYLGADREAWKQHDASELVARADAPKFADGILVDQGLADQFLANQLNPDVFE
CCCHHHHHCCCCCCHHHHHHCCHHHHHHHCCCCCHHCCEEECCCHHHHHHHHCCCHHHHH
AACAKAGQPLTLRRHPGYDHGYYFISTFIADHIAHHARVLMR
HHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]