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The map label for this gene is tuf1 [H]

Identifier: 161523427

GI number: 161523427

Start: 287120

End: 288310

Strand: Direct

Name: tuf1 [H]

Synonym: Bmul_0247

Alternate gene names: 161523427

Gene position: 287120-288310 (Clockwise)

Preceding gene: 161523426

Following gene: 161523428

Centisome position: 8.33

GC content: 61.8

Gene sequence:

>1191_bases
ATGGCAAAAGAGAAGTTTGAGCGGACCAAGCCGCACGTGAACGTTGGTACGATTGGTCACGTTGACCACGGCAAGACGAC
GCTGACGGCGGCGATCGCGACGGTTCTGTCGTCGAAGTTCGGCGGCGAAGCGAAGAAGTACGACGAAATCGACGCGGCGC
CGGAAGAAAAGGCACGCGGCATCACGATCAACACCGCGCACATCGAGTACGAAACGGCGAACCGCCACTACGCGCACGTC
GACTGCCCGGGCCACGCCGACTACGTGAAGAACATGATCACGGGTGCAGCGCAGATGGACGGCGCGATCCTGGTGTGCTC
GGCCGCAGACGGCCCGATGCCGCAAACGCGTGAGCACATCCTGCTGGCGCGTCAGGTTGGCGTTCCGTACATCATCGTGT
TCCTGAACAAGTGCGACATGGTGGACGACGCTGAACTGCTCGAGCTGGTCGAGATGGAAGTTCGCGAACTGCTGTCGAAG
TACGACTTCCCGGGCGACGACACGCCGATCATCAAGGGTTCGGCAAAGCTGGCGCTGGAAGGCGACAAGGGCGAGCTGGG
CGAAACGGCGATCATGAACCTGGCCGACGCGCTGGACACGTACATCCCGACGCCGGAGCGCGCGGTCGACGGTACGTTCC
TGATGCCGGTGGAAGACGTGTTCTCGATCTCGGGCCGCGGTACGGTGGTGACGGGTCGTGTCGAGCGCGGTGTGATCAAG
GTCGGTGAGGAAATCGAAATCGTCGGTATCAAGCCGACGGTGAAGACGACCTGCACGGGCGTTGAAATGTTCCGCAAGCT
GCTGGACCAGGGTCAGGCAGGCGACAACGTCGGTATCCTGCTGCGCGGCACGAAGCGTGAAGACGTGGAGCGTGGCCAGG
TTCTGGCGAAGCCGGGTTCGATCACGCCGCACACGCACTTCACGGCCGAAGTGTACGTGCTGAGCAAGGACGAAGGCGGC
CGTCACACGCCGTTCTTCAACAACTACCGTCCGCAGTTCTACTTCCGTACGACGGACGTGACGGGCTCGATCGAGCTGCC
GAAGGACAAGGAAATGGTGATGCCGGGCGACAACGTGTCGATCACGGTGAAGCTGATTGCGCCGATCGCGATGGAAGAAG
GTCTGCGCTTCGCTATCCGTGAAGGCGGCCGTACCGTCGGCGCTGGCGTCGTCGCAAAGATCATCGAGTAA

Upstream 100 bases:

>100_bases
CTACGCTGAAGCTCCGCGCAACGTTGCCGACGCGATCATCAGCGCGAAGTCGAAGTAAATCTGCATCTACCAACTGATTA
TTTTTTGAAAGAAGAGAATC

Downstream 100 bases:

>100_bases
ATTCGGCGATCCGCGGCTCCCCACGGGGGCCGCGATTCCACGGTAGCAGTCGTACCGTCCGAAACCGGGCGTCCAGTTCC
GCTGGCGCCCGGTTTCGTTT

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKEKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSSKFGGEAKKYDEIDAAPEEKARGITINTAHIEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSK
YDFPGDDTPIIKGSAKLALEGDKGELGETAIMNLADALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRVERGVIK
VGEEIEIVGIKPTVKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLAKPGSITPHTHFTAEVYVLSKDEGG
RHTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE

Sequences:

>Translated_396_residues
MAKEKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSSKFGGEAKKYDEIDAAPEEKARGITINTAHIEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSK
YDFPGDDTPIIKGSAKLALEGDKGELGETAIMNLADALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRVERGVIK
VGEEIEIVGIKPTVKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLAKPGSITPHTHFTAEVYVLSKDEGG
RHTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE
>Mature_395_residues
AKEKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSSKFGGEAKKYDEIDAAPEEKARGITINTAHIEYETANRHYAHVD
CPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSKY
DFPGDDTPIIKGSAKLALEGDKGELGETAIMNLADALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRVERGVIKV
GEEIEIVGIKPTVKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLAKPGSITPHTHFTAEVYVLSKDEGGR
HTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=57.1065989847716, Blast_Score=486, Evalue=1e-137,
Organism=Homo sapiens, GI223555963, Length=437, Percent_Identity=30.2059496567506, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI5729864, Length=437, Percent_Identity=30.2059496567506, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=28.5714285714286, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=29.4642857142857, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI194018520, Length=434, Percent_Identity=28.3410138248848, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI194018522, Length=434, Percent_Identity=28.3410138248848, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI194097354, Length=434, Percent_Identity=28.3410138248848, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI46094014, Length=439, Percent_Identity=27.5626423690205, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI54607086, Length=330, Percent_Identity=29.0909090909091, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI4503507, Length=268, Percent_Identity=28.3582089552239, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=34.0740740740741, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI53729339, Length=231, Percent_Identity=32.034632034632, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI53729337, Length=231, Percent_Identity=32.034632034632, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI157426893, Length=300, Percent_Identity=26.3333333333333, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=80, Blast_Score=631, Evalue=0.0,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=80, Blast_Score=631, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=341, Percent_Identity=29.6187683284457, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI48994988, Length=274, Percent_Identity=30.2919708029197, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789108, Length=328, Percent_Identity=28.9634146341463, Blast_Score=86, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17556456, Length=403, Percent_Identity=52.8535980148883, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=45.7364341085271, Blast_Score=340, Evalue=6e-94,
Organism=Caenorhabditis elegans, GI17552884, Length=460, Percent_Identity=31.5217391304348, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17569207, Length=460, Percent_Identity=31.5217391304348, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI32566303, Length=441, Percent_Identity=29.7052154195011, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17506081, Length=275, Percent_Identity=32.3636363636364, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI115532067, Length=439, Percent_Identity=26.4236902050114, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI115532065, Length=436, Percent_Identity=26.3761467889908, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI32566629, Length=353, Percent_Identity=28.8951841359773, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=154, Percent_Identity=38.961038961039, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=292, Percent_Identity=26.027397260274, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=237, Percent_Identity=27.0042194092827, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=65.9090909090909, Blast_Score=536, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6325337, Length=445, Percent_Identity=31.4606741573034, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=445, Percent_Identity=31.4606741573034, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6320377, Length=336, Percent_Identity=30.0595238095238, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6322937, Length=358, Percent_Identity=26.8156424581006, Blast_Score=90, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=265, Percent_Identity=27.1698113207547, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=58.2697201017812, Blast_Score=488, Evalue=1e-138,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=58.2697201017812, Blast_Score=488, Evalue=1e-138,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=51.8134715025907, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24652838, Length=455, Percent_Identity=30.3296703296703, Blast_Score=157, Evalue=8e-39,
Organism=Drosophila melanogaster, GI17137572, Length=455, Percent_Identity=30.3296703296703, Blast_Score=157, Evalue=8e-39,
Organism=Drosophila melanogaster, GI45553807, Length=455, Percent_Identity=30.1098901098901, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI45553816, Length=455, Percent_Identity=30.1098901098901, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24651721, Length=455, Percent_Identity=30.1098901098901, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI17864154, Length=455, Percent_Identity=30.1098901098901, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI45550900, Length=448, Percent_Identity=28.125, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI17137380, Length=346, Percent_Identity=31.2138728323699, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19922690, Length=307, Percent_Identity=29.9674267100977, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24647046, Length=269, Percent_Identity=27.8810408921933, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24647048, Length=269, Percent_Identity=27.8810408921933, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28572034, Length=232, Percent_Identity=34.4827586206897, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: NA

Molecular weight: Translated: 43094; Mature: 42963

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSSKFGGEAKKYDEIDAAPEEKARG
CCCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCC
ITINTAHIEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSKYDFPGDDTPIIKGSAKLALE
EEEHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEE
GDKGELGETAIMNLADALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRVERGVIK
CCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHCCCCCCEEEECCCCCCEEE
VGEEIEIVGIKPTVKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLAKPGS
CCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCC
ITPHTHFTAEVYVLSKDEGGRHTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVS
CCCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCCEEECCCCCE
ITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE
EEEEEEECHHHHCCCEEEECCCCCEECCHHHHHHCC
>Mature Secondary Structure 
AKEKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSSKFGGEAKKYDEIDAAPEEKARG
CCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCC
ITINTAHIEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSKYDFPGDDTPIIKGSAKLALE
EEEHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEE
GDKGELGETAIMNLADALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRVERGVIK
CCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHCCCCCCEEEECCCCCCEEE
VGEEIEIVGIKPTVKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLAKPGS
CCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCC
ITPHTHFTAEVYVLSKDEGGRHTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVS
CCCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCCEEECCCCCE
ITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE
EEEEEEECHHHHCCCEEEECCCCCEECCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA