Click here to switch to the map view.

The map label for this gene is yfjR [H]

Identifier: 161523390

GI number: 161523390

Start: 232938

End: 233807

Strand: Direct

Name: yfjR [H]

Synonym: Bmul_0210

Alternate gene names: 161523390

Gene position: 232938-233807 (Clockwise)

Preceding gene: 161523388

Following gene: 161523391

Centisome position: 6.75

GC content: 72.53

Gene sequence:

>870_bases
ATGGATCTCGGATTTATCGGGCTGGGCGAAATGGGGCAGGCAATCGCGACGAACCTGCTGAAGGCCGGACATACGGTGCG
CGTGTGGAACCGTTCGCGCGATCGCGCGGCACCGCTGGCGGCGCTCGGCGCCCAGATCGTCGATGCGCCGGCCGACGCGT
TTCGCGGCGACGCGGTGTTTTCGATGCTGGCCGACGACGCCGCCGCGCGCGCCGTGTTCGACGACGCGCTGCTCGCGCAG
GCGCCGCGCGGCCTGATCCACGTGAACATGGCGACGATATCGGTCGCGCTCGCGGAATCGCTCGCGCACGCGCATGCGTC
GCGCGGCCTGCAGTACGTCGCCGCGCCGGTGATGGGCCGGCCCGACGTCGCGGCCGCCGGGCGCCTGACGATCATGGCCG
GCGGCCCGGCCGAGGCGATCGACCGCGTGCAGCCGCTGTTCGATGCGATCGGCCAGAAGACCTGGCGCTTCGGGGCGCTG
CCGCAGCACGCGAACGTCGCGAAGATCGCGGCGAATTTCACGCTCGCGTCGGCGATCGAGACGCTCGGCGAGGCGTCGGC
GCTGCTCGGCGCGCACGGCGTCGCGATGCGCGACTTCCTCGACGTGATCACGCACAGCGTGTTTCCGGGGCCCGTCTACG
AAGGCTACGGCGCGATGATCGCCGAACGCCGCTACGAGCCGGCGCGCTTCAAGGCGCGGCTCGGGCTGAAGGATGTGCGG
CTCGCGCTCGAAGCCGGCGACGCGGCGTCGGTGCCGCTGCCGGTCGCGAGCGTCGTGCGCGACAGCCTGCTCGACGTGCT
CGCGCACGGCGGCGGCGATCTCGACTTCGCCGCGCTCGGCGACGCGGCACTGCGCCGCGCGGCTCGTTGA

Upstream 100 bases:

>100_bases
ATCGCGCGCCGTGCGGCGTGCGGCGTCAGGCCTGAGGCGCGCGCTGCGCGTTGCACGCATCTGTGTTTAACTCGCGGCTT
GACGATCGAAGGAGCGGGCA

Downstream 100 bases:

>100_bases
CGGCGCGCGCGACAGCAGCGTCGCGCGCACGGCATAATCGACGTTTCGTTTTGACGACTTCACGCAGAGGTGCGCAATGA
ACCTGCATACCTGGTGGCTT

Product: 6-phosphogluconate dehydrogenase

Products: (R)-Glycerate; Nicotinamide adenine dinucleotide [C]

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MDLGFIGLGEMGQAIATNLLKAGHTVRVWNRSRDRAAPLAALGAQIVDAPADAFRGDAVFSMLADDAAARAVFDDALLAQ
APRGLIHVNMATISVALAESLAHAHASRGLQYVAAPVMGRPDVAAAGRLTIMAGGPAEAIDRVQPLFDAIGQKTWRFGAL
PQHANVAKIAANFTLASAIETLGEASALLGAHGVAMRDFLDVITHSVFPGPVYEGYGAMIAERRYEPARFKARLGLKDVR
LALEAGDAASVPLPVASVVRDSLLDVLAHGGGDLDFAALGDAALRRAAR

Sequences:

>Translated_289_residues
MDLGFIGLGEMGQAIATNLLKAGHTVRVWNRSRDRAAPLAALGAQIVDAPADAFRGDAVFSMLADDAAARAVFDDALLAQ
APRGLIHVNMATISVALAESLAHAHASRGLQYVAAPVMGRPDVAAAGRLTIMAGGPAEAIDRVQPLFDAIGQKTWRFGAL
PQHANVAKIAANFTLASAIETLGEASALLGAHGVAMRDFLDVITHSVFPGPVYEGYGAMIAERRYEPARFKARLGLKDVR
LALEAGDAASVPLPVASVVRDSLLDVLAHGGGDLDFAALGDAALRRAAR
>Mature_289_residues
MDLGFIGLGEMGQAIATNLLKAGHTVRVWNRSRDRAAPLAALGAQIVDAPADAFRGDAVFSMLADDAAARAVFDDALLAQ
APRGLIHVNMATISVALAESLAHAHASRGLQYVAAPVMGRPDVAAAGRLTIMAGGPAEAIDRVQPLFDAIGQKTWRFGAL
PQHANVAKIAANFTLASAIETLGEASALLGAHGVAMRDFLDVITHSVFPGPVYEGYGAMIAERRYEPARFKARLGLKDVR
LALEAGDAASVPLPVASVVRDSLLDVLAHGGGDLDFAALGDAALRRAAR

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=282, Percent_Identity=29.4326241134752, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI23308751, Length=289, Percent_Identity=26.2975778546713, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1786719, Length=285, Percent_Identity=30.8771929824561, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI145693186, Length=282, Percent_Identity=28.0141843971631, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789092, Length=282, Percent_Identity=28.3687943262411, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1790315, Length=282, Percent_Identity=25.886524822695, Blast_Score=74, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24655230, Length=287, Percent_Identity=28.5714285714286, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19922568, Length=287, Percent_Identity=28.5714285714286, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI28574115, Length=267, Percent_Identity=27.3408239700375, Blast_Score=105, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.60 [C]

Molecular weight: Translated: 30069; Mature: 30069

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLGFIGLGEMGQAIATNLLKAGHTVRVWNRSRDRAAPLAALGAQIVDAPADAFRGDAVF
CCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
SMLADDAAARAVFDDALLAQAPRGLIHVNMATISVALAESLAHAHASRGLQYVAAPVMGR
HHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCHHEEHHCCCCC
PDVAAAGRLTIMAGGPAEAIDRVQPLFDAIGQKTWRFGALPQHANVAKIAANFTLASAIE
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCHHEECCCCCCCCCHHHHHHHHHHHHHHH
TLGEASALLGAHGVAMRDFLDVITHSVFPGPVYEGYGAMIAERRYEPARFKARLGLKDVR
HHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHCHHHHHHHHCCCHHHHHHHCCHHHHH
LALEAGDAASVPLPVASVVRDSLLDVLAHGGGDLDFAALGDAALRRAAR
HHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MDLGFIGLGEMGQAIATNLLKAGHTVRVWNRSRDRAAPLAALGAQIVDAPADAFRGDAVF
CCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
SMLADDAAARAVFDDALLAQAPRGLIHVNMATISVALAESLAHAHASRGLQYVAAPVMGR
HHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCHHEEHHCCCCC
PDVAAAGRLTIMAGGPAEAIDRVQPLFDAIGQKTWRFGALPQHANVAKIAANFTLASAIE
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCHHEECCCCCCCCCHHHHHHHHHHHHHHH
TLGEASALLGAHGVAMRDFLDVITHSVFPGPVYEGYGAMIAERRYEPARFKARLGLKDVR
HHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHCHHHHHHHHCCCHHHHHHHCCHHHHH
LALEAGDAASVPLPVASVVRDSLLDVLAHGGGDLDFAALGDAALRRAAR
HHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Hydroxy-3-oxopropanoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Specific reaction: 2-Hydroxy-3-oxopropanoate + H+ + Nicotinamide adenine dinucleotide - reduced --> (R)-Glycerate + Nicotinamide adenine dinucleotide [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]