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The map label for this gene is rhtB [H]

Identifier: 161523391

GI number: 161523391

Start: 233884

End: 234522

Strand: Direct

Name: rhtB [H]

Synonym: Bmul_0211

Alternate gene names: 161523391

Gene position: 233884-234522 (Clockwise)

Preceding gene: 161523390

Following gene: 161523393

Centisome position: 6.78

GC content: 68.23

Gene sequence:

>639_bases
ATGAACCTGCATACCTGGTGGCTTTTCGTGGCGACGGTGTTCGTCGTGTCGGCGATTCCCGGACCGAACATGCTGCTGGT
GATGACGCACGGCGCGCGCCACGGGCTGCGGCGCTCGGCGGCGACGATGGCCGGTTGTCTGAGCGCGCTCGTGCTGATGC
TGTCGGTGTCGGCCGCGGGCCTCGGCGTGTTTCTCGAGGCGTGGCCCGCGATGTTCAACGCGCTGCGTTTCGCGGGCGCC
GCCTATCTGATTTACCTCGGCGTGAAGGCGTGGCGCGCAAGCGCCGACGACGTGCCGGCCGCCGATGCCGAGCCCGCGCT
GCGCCATGCGGCCAATGCGTCGCGCTGGGCGCTGTTCCGCAACGGCTTCCTGGTGGCGGGCAGCAACCCGAAGGCGATCC
TGTTCGCGGCCGCGCTGCTGCCGCAGTTCATCAACGCGGCCGAGCCGACGCTGCCGCAGTTCGGCATGCTCGTCGTCACG
TTCGCCGTGATCGAGGTCAGCTGGTATCTCGTCTATGCGTCGTTCGGCACGCGCATCGGTGCGACGCTGAAAAGCCGCAG
CGTCGCGAAGATCTTCAACCGGCTGACGGGCGGACTGTTCGTCGGCTTCGGCGCGATGATGGCGCTGGTCCGCCACTGA

Upstream 100 bases:

>100_bases
GCACTGCGCCGCGCGGCTCGTTGACGGCGCGCGCGACAGCAGCGTCGCGCGCACGGCATAATCGACGTTTCGTTTTGACG
ACTTCACGCAGAGGTGCGCA

Downstream 100 bases:

>100_bases
TTCGCACCGCCGCCGCGCGCCGAACGACCCACACCGAACGCCCCGCGCCTCGTCGGAGGGCGGGGCGTTCGTCTTTCAGG
TCTGCGTGTCGGGCAGTGCC

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MNLHTWWLFVATVFVVSAIPGPNMLLVMTHGARHGLRRSAATMAGCLSALVLMLSVSAAGLGVFLEAWPAMFNALRFAGA
AYLIYLGVKAWRASADDVPAADAEPALRHAANASRWALFRNGFLVAGSNPKAILFAAALLPQFINAAEPTLPQFGMLVVT
FAVIEVSWYLVYASFGTRIGATLKSRSVAKIFNRLTGGLFVGFGAMMALVRH

Sequences:

>Translated_212_residues
MNLHTWWLFVATVFVVSAIPGPNMLLVMTHGARHGLRRSAATMAGCLSALVLMLSVSAAGLGVFLEAWPAMFNALRFAGA
AYLIYLGVKAWRASADDVPAADAEPALRHAANASRWALFRNGFLVAGSNPKAILFAAALLPQFINAAEPTLPQFGMLVVT
FAVIEVSWYLVYASFGTRIGATLKSRSVAKIFNRLTGGLFVGFGAMMALVRH
>Mature_212_residues
MNLHTWWLFVATVFVVSAIPGPNMLLVMTHGARHGLRRSAATMAGCLSALVLMLSVSAAGLGVFLEAWPAMFNALRFAGA
AYLIYLGVKAWRASADDVPAADAEPALRHAANASRWALFRNGFLVAGSNPKAILFAAALLPQFINAAEPTLPQFGMLVVT
FAVIEVSWYLVYASFGTRIGATLKSRSVAKIFNRLTGGLFVGFGAMMALVRH

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=213, Percent_Identity=34.7417840375587, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1788099, Length=215, Percent_Identity=29.3023255813954, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22685; Mature: 22685

Theoretical pI: Translated: 11.16; Mature: 11.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLHTWWLFVATVFVVSAIPGPNMLLVMTHGARHGLRRSAATMAGCLSALVLMLSVSAAG
CCCHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGVFLEAWPAMFNALRFAGAAYLIYLGVKAWRASADDVPAADAEPALRHAANASRWALFR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCHHHHHEE
NGFLVAGSNPKAILFAAALLPQFINAAEPTLPQFGMLVVTFAVIEVSWYLVYASFGTRIG
CCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATLKSRSVAKIFNRLTGGLFVGFGAMMALVRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLHTWWLFVATVFVVSAIPGPNMLLVMTHGARHGLRRSAATMAGCLSALVLMLSVSAAG
CCCHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGVFLEAWPAMFNALRFAGAAYLIYLGVKAWRASADDVPAADAEPALRHAANASRWALFR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCHHHHHEE
NGFLVAGSNPKAILFAAALLPQFINAAEPTLPQFGMLVVTFAVIEVSWYLVYASFGTRIG
CCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATLKSRSVAKIFNRLTGGLFVGFGAMMALVRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]